BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0199.0001.p (620 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3808 [J] COG0018 Arginyl-tRNA synthetase 162 2e-39 BH0834 [J] COG0018 Arginyl-tRNA synthetase 118 3e-26 YDR341c [J] COG0018 Arginyl-tRNA synthetase 89 3e-17 YHR091c [J] COG0018 Arginyl-tRNA synthetase 72 3e-12 >BH3808 [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 162 bits (409), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 0/649 (0%), Positives = 0/649 (0%), Gaps = 129/649 (19%) Query: 4 QWIKNQLEQCAANVINSLGEASAEP-ATVLLEQPPNADFGDYSSNIAMQLAKKLRKTPMA 62 + + LE + A + M +T Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 63 VAGLITAEFQRIGAAGGIVQRIEIANPGFINLYIN---WQEWAGQTFKLPP-------GS 112 + + G+ + I + + + + A + Sbjct: 65 TETYVGNWLAKHGSR---EKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 113 GEKVIIEHTSVNPNKSLHIGHLRNSCIGDALVRILRKTGHNVEVHNYVDDLGNQLADTVV 172 + + P + + D+ + + + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 173 GLLRVPLAGEHVRFGDYCWDIYAAVNKKYAEQPEMIRQRTEILH---ELEEGHGNT---- 225 I E Sbjct: 182 TA--------FGVM-------RYLHLAD-KHDLPRIVTIQNPYSLLNRSFEVGLAEVSQY 225 Query: 226 ------AWLGQLAAERIVREHVAEMRSFGIEYDLLVWESSIVQAGFWSSASGLLKETDMF 279 A+ + + + + + A+ + Sbjct: 226 EGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRH- 284 Query: 280 VQETEGKLAGCWILKQPAAEGMENGSEDHQKDKVLVRSNGILTYTAKDIAYHLWKFGLLD 339 + +L + T + + Sbjct: 285 GLDPAQMA---LA------FVRRQPFV---ASTLLGATTMDQLKTNIESLHLELSE---- 328 Query: 340 KDFTYSEFNAGLWTTGLTGAPKSFGKADRVVNV--IDYRQEYPQAMVKQALEVLGFTAQA 397 A Sbjct: 329 ----------------DVLAEI-------EAVHQV---- 361 Query: 398 EKLHHVSYGVVSLSPASAAGLGIDTSDGKSSYAMSGRQGIGVKVADLVQLMESSIEAARS 457 Sbjct: 362 ----------------------- 398 Query: 458 DVTGLSSRLIATAAIRYYLLRFNLGTEIVFDFKQATEISGNTGV-YLMYTYARAGRVLSK 516 Sbjct: 399 -------- 450 Query: 517 AEDPA----ELTAVPRFPAQLDTAELALLRQLSTWEDTLYTAGRELTPNTICSYAHTLAS 572 Sbjct: 451 --- 507 Query: 573 LFNNFYSSCPILKGE-ESSIRFRLWLTLRFQSTLGEVLELLGLPKPERM 620 Sbjct: 508 556 >BH0834 [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 118 bits (295), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 0/660 (0%), Positives = 0/660 (0%), Gaps = 139/660 (21%) Query: 1 MLSQWIKNQLEQCAANVINSLGEASAEPATVLLEQPPNADFGDYSSNIAMQLAKKLRKTP 60 M I + + +++LG + + Q + Sbjct: 1 MQYHRIPHSSLE-----VSTLGLGTMTF---GEQNSEADAHAQLDYA-VAQGINLIDVAE 51 Query: 61 MAVAGLITAEFQRIGAAGGIVQRIEI---ANPGFINLYINWQEWAGQTFK---------L 108 M + Sbjct: 52 MYPVPPRPETQ---------GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD 102 Query: 109 PPGSGEKVIIEHTSVNPNKSLHIGHLRNSCIGDALVRILRKTGHNVEVHNYVDDLGNQLA 168 + + L K G++ L + L Sbjct: 103 QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLD 162 Query: 169 DTV---------VGLLRVPLAGEHVRFGDYCWDIYAAVNKKYAEQPEMIRQRTEILHELE 219 + A Y + K+ + Q L Sbjct: 163 ALAEYQRAGKIRYIGVSNETAF--------GVMRYLHLADKHDLPRIVTIQNPYSLLNRS 214 Query: 220 EGHGNTAWLGQLAAERIVREHVAEMRSFGIEYDLLVWES-----------SIVQAGFWSS 268 G E + + G + + + Sbjct: 215 FEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 Query: 269 ASGLLKETDMFVQETEGKLAGCWILKQPAAEGMENGSEDHQKDKVLVRSNGILTYTAKDI 328 A+ + + A + + + Sbjct: 275 AAYVD-----IARRHGLDPAQMAL----------------------AFVRRQPFVASTLL 307 Query: 329 AYHLWKFGLLDKDFTYSEFNAGLWTTGLTGAPKSFGKADRVVNVIDYRQEYPQAMVKQAL 388 + + + V Q Sbjct: 308 GATTMDQLK--------------------------TNIESLHLELSEDVLAEIEAVHQVY 341 Query: 389 EVLGFTAQAEKLHHVSYGVVSLSPASAAGLGIDTSDGKSSYAMSGRQGIGVKVA----DL 444 Sbjct: 342 ----------------- 384 Query: 445 VQLMESSIEAARSDVTGLS--SRLIATAAIRYYLLRFNLGTEIVFDFKQATEISGNTGVY 502 Sbjct: 385 444 Query: 503 LMYTYARAGRVLSKAEDPAELTAVPRFPAQLDTAE--LALLRQLSTWEDTLYTAGRELTP 560 Sbjct: 445 --- 501 Query: 561 NTICSYAHTLASLFNNFYSSCPILKGEESSIRFRLWLTLRFQSTLGEVLELLGLPKPERM 620 Sbjct: 502 561 >YDR341c [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 88.6 bits (218), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 0/571 (0%), Positives = 0/571 (0%), Gaps = 86/571 (15%) Query: 77 AGGIVQRIEIANPGFINLYINWQEWAGQ------TFKLPPGS-----GEKVIIEHTSVNP 125 GI + I ++ Q P G+ S Sbjct: 96 DKGIRPDQA-LDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPA 154 Query: 126 NKSLHIGHLRNSCIGDALVRILRKTGHNVEVHNYVDDLGNQLADTVVGLLRVPLAGEHVR 185 L +R + + L ++ + ++ + Sbjct: 155 VSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQ--NPYSLLN 212 Query: 186 FGD---YCWDIYAAVNKKYAEQPEMI--RQRT-----EILHELEEGHGNTAWLGQLAAER 235 + A + ++ Sbjct: 213 RSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQK 272 Query: 236 IVREHVAEMRSFGIEYDLLVWESSIVQAGFWSSASGLLKETDMFVQETEGKLAGCWILKQ 295 V +V R G++ + + + K + + Sbjct: 273 AVAAYVDIARRHGLDPAQMALAFVR-------RQPFVASTLLGATTMDQLKTNIESLHLE 325 Query: 296 PAAEGMENGSEDHQKDKVLVRSNGILTYTAKDIAYHLWKFGLLDKDFTYSEFNAGLWTTG 355 + + + Sbjct: 326 LSEDVLAEIE---------------------- 363 Query: 356 LTGAPKSFGKADRVVNVIDYRQEYPQAMVKQALEVLGFTAQAEKLHHVSYGVVSLSPASA 415 Sbjct: 364 ----------------- 406 Query: 416 AGLGIDTSDGKSSYAMSGRQGIGVKVADLVQLMESS---IEAAR--SDVTGLSSRLIATA 470 Sbjct: 407 --- 463 Query: 471 AIRYYLLRFNLGTEIVFDFKQATEISGNTGVYLMYTYARAGRVLSKAEDPAELTAV-PRF 529 Sbjct: 464 ------- 516 Query: 530 PAQLDTAELALLRQLSTWEDTLYTAGRELTPNTICSYAHTLASLFNNFYSSCPILKGEES 589 Sbjct: 517 576 Query: 590 SIRFRLWLTLRFQSTLGEVLELLGLPKPERM 620 Sbjct: 577 607 >YHR091c [J] COG0018 Arginyl-tRNA synthetase Length = 346 Score = 71.6 bits (174), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 0/532 (0%), Positives = 0/532 (0%), Gaps = 92/532 (17%) Query: 114 EKVIIEHTSVNPNKSLHIGHLRNSCIGDALVRILRKTGHNVEVHNYVDDLGNQLADTVVG 173 + + R I + + R + + + + Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLA-----Y 233 Query: 174 LLRVPLAGEHVRFGD----------YCWDIYAAVNKKYAEQPEMIRQRTEI-------LH 216 G + + + +I Sbjct: 234 SCLGFGT----LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPA 289 Query: 217 ELEEGHGNTAWLGQLAAERIVREHVAEMRSFGIEYDLLVWESSIVQAGFWSSASGLLKET 276 ++ + + +L + ++A Sbjct: 290 QMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH- 348 Query: 277 DMFVQETEGKLAGCWILKQPAAEGMENGSEDHQKDKVLV-RSNGILTYTAKDIAYHLWKF 335 Sbjct: 349 -------------------- 388 Query: 336 GLLDKDFTYSEFNAGLWTTGLTGAPKSFGKADRVVNVIDYRQEYPQAMVKQALEVLGFTA 395 Sbjct: 389 -------------------- 428 Query: 396 QAEKLHHVSYGVVSLSPASAAGLGIDTSDGKSSYAMSGRQGIGVKVADLVQLMESS-IEA 454 Sbjct: 429 ------------ 476 Query: 455 ARSDVTGLSSRLIATAAIRYYLLRFNLGTEIVFDFKQATEISGNTGVYLMYTYARAGRVL 514 Sbjct: 477 536 Query: 515 SKAEDPAELTAVPRFPAQLDTAELA-LLRQLSTWEDTLYTAGRELTPNTICSY----AHT 569 Sbjct: 537 596 Query: 570 LASLFNNFYSSCPILKGEESSIRF-RLWLTLRFQSTLGEVLELLGLPKPERM 620 Sbjct: 597 ----- 643 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 46,578,266 Number of extensions: 1959328 Number of successful extensions: 3956 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3952 Number of HSP's successfully gapped: 6 Length of query: 620 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 508 Effective length of database: 25,461,023 Effective search space: 12934199684 Effective search space used: 12934199684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)