BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0015.p (330 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0237 [R] COG0673 Predicted dehydrogenases and related proteins 83 8e-16 BH1248 [R] COG0673 Predicted dehydrogenases and related proteins 78 2e-14 BMEI0420 [R] COG0673 Predicted dehydrogenases and related proteins 72 1e-12 BH0710 [R] COG0673 Predicted dehydrogenases and related proteins 70 6e-12 STM4425 [R] COG0673 Predicted dehydrogenases and related proteins 69 9e-12 >PA0237 [R] COG0673 Predicted dehydrogenases and related proteins Length = 347 Score = 82.8 bits (203), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 70/249 (28%), Positives = 114/249 (45%), Gaps = 42/249 (16%) Query: 5 KLSFIGAGFHASTNIYPSAIQAGAEIVAVATRKLERSRAALQRFGS-SGLPY-SDYRKML 62 +++ IGAG + + AGA + AVA + Q F + +G+P+ D+R++L Sbjct: 6 RIALIGAGNMGRQHYHRLQRIAGARLCAVADPQG-------QAFAADTGVPWFGDHRRLL 58 Query: 63 QDTECDGVVVVAQPQDQTALVLDCIHAGKNVYVDKPLGWNGPXXXXXXXXXXXXGVVLMV 122 ++ +V A LDC+ AG + ++KP+G GV L+V Sbjct: 59 EEARPQAAIVANPNNLHVATALDCLAAGVPLLLEKPVGVQLDEVSELVAVARRSGVPLLV 118 Query: 123 GFMKRYAPVYLKLKELIAEGTLGAARSFQARFAVDSTPFCKDEEQFLKLA---------- 172 G +R+ P+ ++ ++LIAEG LG S A + + K + + ++A Sbjct: 119 GHHRRHNPLVVRARQLIAEGALGRLLSVTALWQL------KKPDSYFEVAWRREPGAGML 172 Query: 173 ---AIHIVDLLRFLFGEAVQ--------ISGFTNNDNERIAQSISLKFANGVVGSVYF-- 219 IH +DLLR L GE + I G N DN ++ L+FANG +GS+ Sbjct: 173 LTNLIHDLDLLRHLCGEVREVQALAGNAIRGLPNEDN----IALLLRFANGALGSLTGCD 228 Query: 220 AGMSAWSRE 228 A + WS E Sbjct: 229 AAAAPWSWE 237 >BH1248 [R] COG0673 Predicted dehydrogenases and related proteins Length = 340 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 85/346 (24%), Positives = 154/346 (44%), Gaps = 41/346 (11%) Query: 5 KLSFIGAGFHASTNIYPSAIQ-AGAEIVAVATRKLERSRAALQRFGSSGLPYSDYRKMLQ 63 +++ IG G A P Q IVAV ER++ + +G+ + Y+D+ ++L+ Sbjct: 5 RVAVIGCGSIAQHRHLPEYEQNPHTTIVAVCDPVEERAKKVAKLYGA--IAYTDHMQLLE 62 Query: 64 DTECDGVVVVAQPQDQTALVLDCIHAGKNVYVDKPLGWNGPXXXXXXXXXXXXGVVLMVG 123 E D V V + +D + AG +V +KP+ + G+ LM+G Sbjct: 63 KEEIDLVSVCTPNAYHAPISIDALKAGTHVLCEKPMATSAEECEAMIKAAEEAGMCLMIG 122 Query: 124 FMKRYAPVYLKLKELIAEGTLGAARSFQARF--------AVD--STPFCKDEEQFLKLA- 172 +R+ + K ++LIA G +G SF+ F +VD ++ F K ++ F+ Sbjct: 123 HNQRFVSSHEKARQLIATGDIGKIYSFRTTFGHGGPEGWSVDGENSWFFKKDQAFIGAMG 182 Query: 173 --AIHIVDLLRFLFG-EAVQISGFTNN-----DNERIAQSISLKFANGVVGSVYFAGMSA 224 +H DLLR+L G E V++ F D + A +I LK +G+VG++ ++ Sbjct: 183 DLGVHKADLLRYLLGEEVVEVGAFVTTSAKQGDVDDNAVAI-LKTESGIVGTL----TAS 237 Query: 225 WSRESESLTVTFDHGFVCADEVVKLS--VHHSLAGSGLPWQSLAEQDMVFTPSASAMSGG 282 WS ++ T +G + +++L H+SL + +++ S GG Sbjct: 238 WSYVAKEDNSTILYG---ENAILRLEDDPHYSLI---VHYKNGETVKYELGQIQSNDEGG 291 Query: 283 YRDLYLRGFAGEMAHFMKCCREGLTPVSSGRDNVKTMELCDAILSA 328 D + +AHF+ EG P SG++ ++ + A + A Sbjct: 292 QTDSRV------IAHFVNSVLEGKEPPISGKEGYASLNIILAAMEA 331 >BMEI0420 [R] COG0673 Predicted dehydrogenases and related proteins Length = 332 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 70/328 (21%), Positives = 138/328 (42%), Gaps = 32/328 (9%) Query: 5 KLSFIGAGFHASTNIYPSAIQAGAEIVAVATRKLERSRAALQRFGSS-GLPYSDYRKMLQ 63 +++ +G G+ +I +++ + AV+ E++ +RF S +P ++ Sbjct: 18 RIAVLGCGYWGGNHI--RTLKSLGALQAVSDSNAEKA----ERFASEFNVPSIPVEELFI 71 Query: 64 DTECDGVVVVAQPQDQTALVLDCIHAGKNVYVDKPLGWNGPXXXXXXXXXXXXGVVLMVG 123 + DG+V+ PQ ++ + GK+V V+KP+ + P G V MVG Sbjct: 72 HPDIDGIVLALPPQLHAQYAMEAVKNGKDVLVEKPIALDIPVALAEVEAARENGRVFMVG 131 Query: 124 FMKRYAPVYLKLKELIAEGTLGAARSFQARFAVDSTPFCKDEEQFLKLAAIHIVDLLRFL 183 + R+ P + KL +++ G LG R + V F + + LA H + ++ + Sbjct: 132 HVLRFHPAFEKLLDMVQSGELGDIRYVHSH-RVGLGKFHTEFDALWDLAP-HDLSMILAI 189 Query: 184 FGE---AVQISGFTNNDNERIAQSISLKFANGVVGSVYFAGMSAWSRESESLTVT----- 235 GE V+ G D+ + ++F +G+ G ++ + ++A+ S+T T Sbjct: 190 TGEEPNVVRGEGVAILDHLNDFAHLHMEFPSGIRGHLFASRLNAYRERRLSVTGTKGMAV 249 Query: 236 FDHGFVCADEVVKLSVH-HSLAGSGLPWQSLAEQDMVFTPSASAMSGGYRDLYLRGFAGE 294 FD G KL+++ H + W + D V+ + M E Sbjct: 250 FDDGEAWER---KLALYRHEVWRENDRW-AFKSADPVYIQTEEGMP----------LTRE 295 Query: 295 MAHFMKCCREGLTPVSSGRDNVKTMELC 322 + HFM C TP + G++ + + + Sbjct: 296 LQHFMHCIETRETPRTDGKEAISVLRIL 323 >BH0710 [R] COG0673 Predicted dehydrogenases and related proteins Length = 388 Score = 69.7 bits (169), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 70/313 (22%), Positives = 123/313 (39%), Gaps = 44/313 (14%) Query: 56 SDYRKMLQDTECDGVVVVAQPQDQTALVLDCIHAGKNVYVDKPLGWNGPXXXXXXXXXXX 115 ++++ +++ + D + + A + L AGK+++ +KPL Sbjct: 62 TNWKDLIRRDDIDLIDINAPSDVHKEITLAAAQAGKHIFCEKPLALTLQDSRHMLEAVEA 121 Query: 116 XGVVLMVGFMKRYAPVYLKLKELIAEGTLGAARSFQARFAVDSTPFCKDEEQFLKLA--- 172 GV MVGF R+ P L K+LIAEG LG F+A F D D +L Sbjct: 122 AGVKHMVGFNYRFTPAVLLAKKLIAEGRLGDIYHFRAWFLQDWI-VAPDFPLVWRLQKDI 180 Query: 173 ---------AIHIVDLLRFLFGEAVQI--------------------SGFTNNDNERIAQ 203 H++D+ FL G+ ++ SG +ND+ Sbjct: 181 AGSGAHGDLGAHLIDMAHFLIGDITEVIGMNETFIKERPLPTEMTGLSGRGSNDHTVPKG 240 Query: 204 SISL--------KFANGVVGSVYFAGMSAWSRESESLTVTFDHGFVCAD--EVVKLSVHH 253 +++ +FANG +GS + R + S + G V D + +L V+ Sbjct: 241 PVTVDDATLFMARFANGALGSFEATRFATGHRSTNSFEINGSKGSVIFDFERLNELQVYF 300 Query: 254 SLAGSGLP-WQSLAEQDMVFTPSASAMSGGYRDLYLRGFAGEMAHFMKCCREGLTPVSSG 312 + + ++ + D S G+ Y F E+A M+ +EG PV + Sbjct: 301 TDDQDDVQGFRRVLATDGAHAYSDRWWPAGHTIGYEHTFIHEIAELMESLQEGRQPVPNF 360 Query: 313 RDNVKTMELCDAI 325 +D VK ++ +A+ Sbjct: 361 KDGVKCQQVLEAV 373 >STM4425 [R] COG0673 Predicted dehydrogenases and related proteins Length = 336 Score = 69.3 bits (168), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 42/162 (25%), Positives = 75/162 (46%), Gaps = 1/162 (0%) Query: 26 AGAEIVAVATRKLERSRAALQRFGSSGLPYSDYRKMLQDTECDGVVVVAQPQDQTALVLD 85 +G E+VAV R++AAL ++ Y+DY ++ D + + V++ A + + + Sbjct: 26 SGVEVVAVCDIVAGRAQAALDKYAIEAKDYNDYHDLINDKDVEVVIITASNEAHADVAVA 85 Query: 86 CIHAGKNVYVDKPLGWNGPXXXXXXXXXXXXGV-VLMVGFMKRYAPVYLKLKELIAEGTL 144 ++A K V+ +KPL G ++ +GFM+RY Y++LK +I G + Sbjct: 86 ALNANKYVFCEKPLAVTAADCQRVIEAEQKNGKRMVQIGFMRRYDKGYVQLKNIIDSGEI 145 Query: 145 GAARSFQARFAVDSTPFCKDEEQFLKLAAIHIVDLLRFLFGE 186 G R ST Q + IH +D++ +L E Sbjct: 146 GQPLMVHGRHYNASTVPEYKTPQAIYETLIHEIDVMHWLLNE 187 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,189,750 Number of extensions: 928542 Number of successful extensions: 7763 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 7790 Number of HSP's successfully gapped: 62 Length of query: 330 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 223 Effective length of database: 26,037,693 Effective search space: 5806405539 Effective search space used: 5806405539 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)