BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0010.p (430 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2670 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 128 1e-29 lin1867 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 117 3e-26 slr0877 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 115 1e-25 all1053 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 112 1e-24 CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 108 1e-23 >CAC2670 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 485 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 114/459 (24%), Positives = 187/459 (40%), Gaps = 55/459 (11%) Query: 11 KDIPDRMQNKLHPSWDLFRERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKP 70 KD+ + + K+ + F R E ++ ++A A E AK L E+ Sbjct: 12 KDMISKKEVKVEEVTNSFLNRIEEVDEKVNALLYVAK--EEAVNTAKELDKKIESGESLS 69 Query: 71 AFYGVPVGIKDLLHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAY 130 GVPV IKD + V+ + S + +S + ++I L+ +I GK +EFA Sbjct: 70 GLSGVPVAIKDNISVKNMQNTCASKILEGYVSPYDATVIENLKKNNGVIIGKANMDEFAM 129 Query: 131 NGPI------ATRNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVV 184 ++NP L P T+T SV PASFCG+V Sbjct: 130 GSSTENSAFKVSKNPWSLERVPGGSSGGSAVAVASLEAPISLGTETGGSVRQPASFCGLV 189 Query: 185 GFKPSYGRVPLDGVILLAPSFDTIGFFTQNVSD----MQAAAALL----------IPDWN 230 G KP+YGR+ GV+ + D +G F ++V D Q A L + D++ Sbjct: 190 GLKPTYGRISRYGVVAFGSTLDQVGMFARDVEDCALLTQNIAGLDKMDFTTVDTPVQDYS 249 Query: 231 HGSRQAGRKPVLGIPRGIYMELMSGGVKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYGD 290 + + +GIP+ + E + GV+ A I+ ++ G VK+ ++P D Y Sbjct: 250 KSLNKDLKGRKIGIPKEFFEEGLDEGVREAVKEAIKVFEENGAEVKECSLPLSD---YAL 306 Query: 291 AMLRFLEGEMAREHAAWF------------ERFADIY--------GAPVREAILRGREIT 330 A + A + A F E D+Y G + I+ G + Sbjct: 307 AAYYIISSAEASSNLARFDGVRYGYRDAEAENALDLYVKSRSKGFGEEAKRRIMLGTYVL 366 Query: 331 DTELEMYRRRQARLCREL---ETARAAQGVDLWVSPAQGGTAPKIGEGTG---WPGMTAI 384 ++A R L + RA + D ++P A +IGE T M+ I Sbjct: 367 SKGYYDAYYKKALKVRSLIKNDFQRAFKEFDAIITPTTPTPAFRIGEKTKDVLSMYMSDI 426 Query: 385 WT----FAGCPAVSLPSASIDNLPLGFQCIGSYGQDEAL 419 +T AG P++S+P + LP+G Q +G+Y +++ L Sbjct: 427 YTVPVNIAGIPSISVPCGFVSGLPVGLQIMGNYFKEDTL 465 >lin1867 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 301 Score = 117 bits (293), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 0/478 (0%), Positives = 0/478 (0%), Gaps = 70/478 (14%) Query: 10 AKDIPDRMQNKLHPSWDLFRERYERLEPHIHAFAVEANAG-------ERLDAAAKHLLDT 62 I + + S L + +++ A A + Sbjct: 8 CGFIAIVGRPNVGKSTLLNKLLGQKIS------ITSRKAQTTRHRIVGIHTEGAYQAIYV 61 Query: 63 YRGTEAKPAFYGVPVGIKDLLHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGK 122 T + + + + L L A Sbjct: 62 --DTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPV 119 Query: 123 TVTEEFAYNGPI------ATRNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIA 176 + N + + + Sbjct: 120 ILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHH 179 Query: 177 PASFCGVVGFKPSYGRVPLDGVILLAPSFDTIGFFTQNVSDMQAAAALLIPDWNHGSRQA 236 + + ++ + T + + D N Sbjct: 180 FPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIER-FVSNERGGYDINGLILVE 238 Query: 237 GRKPV---------------LGIPRGIYMELMSGGVKAAFTAQIEGLQQKGYTVKQVNMP 281 + + + + + G ++ + Sbjct: 239 REGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYV 298 Query: 282 WEDEFIYGDAMLRFLEGEMAREHAAWFE--RF------------------ADIYGAPVRE 321 + Sbjct: 299 DDL--- 355 Query: 322 AILRGR-EITDTELEMYRRR--QARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGW 378 Sbjct: 356 415 Query: 379 P-------GMTAIWTFAGCPAVSLPSASIDNLPLGFQCIGSYGQDEALAAWAQQISED 429 Sbjct: 416 473 >slr0877 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 483 Score = 115 bits (288), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 113/396 (28%), Positives = 168/396 (42%), Gaps = 53/396 (13%) Query: 74 GVPVGIKDLLHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAY--- 130 G+P+GIKD L +G++T S + + E ++ +L+ GA++ GKT +EFA Sbjct: 69 GIPIGIKDNLCTKGIVTTCASQILRGFVPPYESTVTEKLQKAGAVMVGKTNLDEFAMGSS 128 Query: 131 ---NGPIATRNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFK 187 +G T NP DL+ P + T S+ PAS CGVVG K Sbjct: 129 TENSGYQVTANPWDLTRVPGGSSGGSAAAVAADECLIALGSDTGGSIRQPASLCGVVGMK 188 Query: 188 PSYGRVPLDGVILLAPSFDTIGFFTQNVSDMQAAAALLIPDWNHGSR------------- 234 P+YG V G++ A S D IG F + V D AA LL H SR Sbjct: 189 PTYGLVSRFGLVAYASSLDQIGPFARRVED---AAILLQAIAGHDSRDSTSLDVPIPDYT 245 Query: 235 QAGRKPVLGIPRGIYMELMSGG----VKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYG- 289 QA + + G+ G+ M+ G V A I L++ G T+++++ P I Sbjct: 246 QALKPDLKGVKVGVIMDAFGEGLDDTVNEAVQTAIAKLRELGATIEEIHCPRFRSGIAAY 305 Query: 290 ---------------DAMLRFLEGEMAREHAAWFERFADIYGAPVREAILRGR-EITDTE 333 DA+ L E + A +GA V+ I+ G ++ Sbjct: 306 YVIAPSEASANLARYDAVRYGLRAEADNLMEMYTHTRAKGFGAEVKRRIMLGTYALSAGY 365 Query: 334 LEMY--RRRQARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGWP-------GMTAI 384 + Y + ++ R + + +A VD+ V P TA K GE T P MT Sbjct: 366 YDAYYLKAQKVRTLIKQDFDQAFTKVDVLVCPTAPTTAFKAGEKTDDPLSMYLSDLMTIP 425 Query: 385 WTFAGCPAVSLPSASID-NLPLGFQCIGSYGQDEAL 419 AG PA+S+P D NLP+G Q +G+ +E L Sbjct: 426 VNLAGLPAMSVPCGFDDNNLPIGLQLVGNVLGEEML 461 >all1053 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 486 Score = 112 bits (280), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 122/451 (27%), Positives = 189/451 (41%), Gaps = 73/451 (16%) Query: 30 ERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGTEAKPA-------FYGVPVGIKDL 82 + + LEP +H+F L A+ L+ R +AK A G+P+G+KD Sbjct: 27 DHIQELEPKLHSF---------LHITAQQALEQARAVDAKIAAGEEIGLLAGIPIGVKDN 77 Query: 83 LHVEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGPI------AT 136 + +G+ T S + + E ++ ++L GA++ GKT +EFA T Sbjct: 78 MCTKGIPTTCASRILENFVPPYESTVTQKLLDAGAVVVGKTNLDEFAMGSSTENSAYQVT 137 Query: 137 RNPHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRVPLD 196 NP DLS P + T S+ PASFCGVVG KP+YG V Sbjct: 138 ANPWDLSRVPGGSSGGSAAAVAAEECVVALGSDTGGSIRQPASFCGVVGLKPTYGLVSRY 197 Query: 197 GVILLAPSFDTIGFFTQNVSD----MQAAAA----------LLIPDWNHGSR---QAGRK 239 G++ A S D IG F ++V D ++A A + IPD+ + +A K Sbjct: 198 GLVAYASSLDQIGPFGKSVEDTAILLKAIAGYDHQDSTSLKVEIPDYVASLKPDLKARSK 257 Query: 240 PVLGIPRGIYMELMSGGVKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYGDAMLRFLEGE 299 +GI + + E + V+ A T IE LQ+ G V ++ P F YG + Sbjct: 258 LRIGIIKETFGEGLDSVVEQAVTKAIEQLQRLGAEVHVISCP---NFRYGVPSYYIIAPS 314 Query: 300 MAREHAAWFERF--------------------ADIYGAPVREAILRGR-EITDTELEMY- 337 A + A ++ A +GA V+ I+ G ++ + Y Sbjct: 315 EASANLARYDGVKYGWRAPEGDNLLSMYKRTRATGFGAEVKRRIMIGTYALSAGYYDAYY 374 Query: 338 -RRRQARLCRELETARAAQGVDLWVSPAQGGTAPKIGEGTGWPG-------MTAIWTFAG 389 + ++ R + + A + VD+ VSP TA K GE T P MT AG Sbjct: 375 LKAQKVRTLIKQDFENAFKNVDVLVSPTAPTTAFKAGEKTADPISMYLNDLMTIPVNLAG 434 Query: 390 CPAVSLPSASIDN-LPLGFQCIGSYGQDEAL 419 P +S+P + LP+G Q IG +++ L Sbjct: 435 LPGLSVPCGFDEQGLPIGLQLIGKVLREDQL 465 >CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Length = 478 Score = 108 bits (271), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 107/439 (24%), Positives = 179/439 (40%), Gaps = 63/439 (14%) Query: 28 FRERYERLEPHIHAFAVEANAGERLDAAAKHLLDTYRGT---EAKPAFYGVPVGIKDLLH 84 + + +R EP I+A+ D A K D + + G+P+ IKD + Sbjct: 29 YFDNIKRNEPEINAYITLCE-----DYALKEAKDVDKKIANGDKVGRLAGIPIAIKDNIC 83 Query: 85 VEGLLTRAGSGLPAAVLSGQEGSLIRRLRSLGALIAGKTVTEEFAYNGPIA------TRN 138 +G+ T S + + + ++I++L++ A+I GK +EFA T+N Sbjct: 84 TDGIKTTCASKMLYDFVPPYDATVIKKLKAEDAIIIGKVNMDEFAMGSSTENSAFKITKN 143 Query: 139 PHDLSHTPXXXXXXXXXXXXXXXXXXXXXTQTLRSVIAPASFCGVVGFKPSYGRVPLDGV 198 P D++ P + T S+ PA+FCGVVG KP+YG V G+ Sbjct: 144 PRDITRVPGGSSGGSAAVVAAKMAPISLGSDTGGSIRQPAAFCGVVGLKPTYGLVSRFGL 203 Query: 199 ILLAPSFDTIGFFTQNVSDMQAAAALLI---------------PDWNHGSRQAGRKPVLG 243 I A S D IG + V D ++ D+ G + +G Sbjct: 204 IAFASSLDQIGPLGKTVKDCAELLEVISGEDELDNTSSKKHEKEDYLEGIDDGIKGMKIG 263 Query: 244 IPRGIYMELMSGGVKAAFTAQIEGLQQKGYTVKQVNMPWEDEFIYGDAMLRFLEGEMARE 303 +P+ + + ++ IE L+ G V ++++P +E G + + A Sbjct: 264 MPKEFLNDGLDPEIRKCIDDTIEKLKSLGAEVCEMSLPITEE---GLSAYYIISSAEASS 320 Query: 304 HAAWF---------ERFADIY-----------GAPVREAILRGR-EITDTELEMYRRRQA 342 + A F + F D+Y G V+ I+ G ++ + Y +R Sbjct: 321 NLARFDGIRYGYRPDDFEDVYDLMETSRSEAFGDEVKRRIMLGTYALSSGYYDAYYKRAL 380 Query: 343 RLCREL--ETARAAQGVDLWVSPAQGGTAPKIGEGTGWP---GMTAIWT----FAGCPAV 393 +L +++ E A + DL +SP KIGE P + I+T AG PA+ Sbjct: 381 KLKKKIKNEFKEAFENYDLILSPVSPVLPFKIGEKKADPLQMYLADIYTVNINLAGIPAI 440 Query: 394 SLP-SASIDNLPLGFQCIG 411 SLP S S + LP+G Q +G Sbjct: 441 SLPCSVSKEGLPIGLQLLG 459 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,862,509 Number of extensions: 1365908 Number of successful extensions: 3025 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 2921 Number of HSP's successfully gapped: 65 Length of query: 430 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 321 Effective length of database: 25,807,025 Effective search space: 8284055025 Effective search space used: 8284055025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)