BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0009.p (418 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_aprX [O] COG1404 Subtilisin-like serine proteases 286 4e-77 BH1930 [O] COG1404 Subtilisin-like serine proteases 283 3e-76 CAC3549 [O] COG1404 Subtilisin-like serine proteases 146 5e-35 BS_aprE [O] COG1404 Subtilisin-like serine proteases 135 1e-31 BH0855 [O] COG1404 Subtilisin-like serine proteases 126 8e-29 >BS_aprX [O] COG1404 Subtilisin-like serine proteases Length = 442 Score = 286 bits (732), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 174/428 (40%), Positives = 247/428 (57%), Gaps = 27/428 (6%) Query: 7 VKQYASKMNRPLRNEIRKTFHSGKSSAA---------------LPVIVQFKHKITPSRML 51 V+ A K++ PLR + + + K + + VI++F+ + Sbjct: 10 VRANAHKLDWPLRETVLQLYKPFKWTPCFLHKFFETKLQNRKKMSVIIEFEEGCHETGFQ 69 Query: 52 ALHKHLGAHSFA-IKHRIPMLNALSSRVSVNCLKRLCC-WEGVHKVHLDHIKSISLDIAT 109 + L + +K R +N S+ V+ + L L + KV+L+ LD AT Sbjct: 70 MAGEVLQKEKRSKLKSRFNKINCCSAEVTPSALHSLLSECSNIRKVYLNREVKALLDTAT 129 Query: 110 PSIGA-AAVQSKRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNG 168 + A V++ + LTGKG+ +AV+DTG++PHPDL RI+ F D VN + PYDDNG Sbjct: 130 EASHAKEVVRNGQTLTGKGVTVAVVDTGIYPHPDLE---GRIIGFADMVNQKTEPYDDNG 186 Query: 169 HGTHITGDAAGNGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCI---AEK 225 HGTH GD A +G SS G+YRGPAPEA ++GVKVL+ G G + II+G+EWCI + Sbjct: 187 HGTHCAGDVASSGASSSGQYRGPAPEANLIGVKVLNKQGSGTLADIIEGVEWCIQYNEDN 246 Query: 226 KRLKLRILSMSFGGPI--STSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGI 283 + I+SMS GG ++D L +AVE+A AG+ V +AAGNSGP +TI SPG+ Sbjct: 247 PDEPIDIMSMSLGGDALRYDHEQEDPLVRAVEEAWSAGIVVCVAAGNSGPDSQTIASPGV 306 Query: 284 SPAAVTVGAVDDRRTLPQKDDRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLAR 343 S +TVGA+DD T DD + +SSRGPT GK KPD++APG ++ISLR+P S + + Sbjct: 307 SEKVITVGALDDNNTASSDDDTVASFSSRGPTVYGKEKPDILAPGVNIISLRSPNSYIDK 366 Query: 344 NFPYLRIGKQYFVLSGTSVSTPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRL-GLKPN 402 R+G QYF +SGTS++TPI +G AA +LQ P LTP +VK LK + PN Sbjct: 367 LQKSSRVGSQYFTMSGTSMATPICAGIAALILQQNPDLTPDEVKELLKNGTDKWKDEDPN 426 Query: 403 TAGSGEIN 410 G+G +N Sbjct: 427 IYGAGAVN 434 >BH1930 [O] COG1404 Subtilisin-like serine proteases Length = 444 Score = 283 bits (725), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 174/428 (40%), Positives = 250/428 (58%), Gaps = 28/428 (6%) Query: 7 VKQYASKMNRPLRNEIRKT----------FHSGKSSAAL-----PVIVQFKHKITPSRML 51 V+ A K+++PLR ++ FH+ L VIV+ + Sbjct: 10 VRSNAHKLDKPLREQMLSMYKPFKWTPCFFHNAFEKMLLKRKTYSVIVELNQGCFDQGSM 69 Query: 52 ALHKHLGAHS-FAIKHRIPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIATP 110 + L H ++H + +S+ V+ L+ + + + K++L+ LD A Sbjct: 70 TVEHVLKKHQKCQLRHSFSKIRCVSANVTPESLQEMLVCKDIRKIYLNREVHALLDTAVE 129 Query: 111 SIGA-AAVQSKRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGH 169 S A +++ LTGK + IAV+DTG++PH DL RI AF DFVN R+ PYDDNGH Sbjct: 130 SAQAFEVIRNGETLTGKDVTIAVIDTGIYPHEDLE---GRIKAFVDFVNQREEPYDDNGH 186 Query: 170 GTHITGDAAGNGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCI---AEKK 226 GTH GDAAGNG SS G+YRGPAPEA ++GVKVL+ G G +I++G+EWCI E Sbjct: 187 GTHCAGDAAGNGASSDGQYRGPAPEANVIGVKVLNKQGMGSLESIMQGVEWCIQYNEEHP 246 Query: 227 RLKLRILSMSFGG---PISTSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGI 283 + I+SMS GG P ++D + + VE+A AG+TV +AAGNSGP +TI SPG+ Sbjct: 247 DDPIHIISMSLGGQALPYENE-QEDPMVRIVEEAWNAGITVCVAAGNSGPDAQTIASPGV 305 Query: 284 SPAAVTVGAVDDRRTLPQKDDRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLAR 343 S +TVGA+DDR T ++DD + +SSRGPT GK KPD++APG +++SLR+P S + Sbjct: 306 SEKVITVGALDDRDTTDREDDDVAPFSSRGPTIYGKPKPDILAPGVNIVSLRSPNSFYDK 365 Query: 344 NFPYLRIGKQYFVLSGTSVSTPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRLGLK-PN 402 R+G Y ++SGTS++TP+ +G A +LQ P+LTP +VK RL R + PN Sbjct: 366 IQKGSRVGSHYTMMSGTSMATPVCAGVVALMLQHEPNLTPDEVKTRLMEGTDRWADRDPN 425 Query: 403 TAGSGEIN 410 G+G I+ Sbjct: 426 VYGAGYIS 433 >CAC3549 [O] COG1404 Subtilisin-like serine proteases Length = 397 Score = 146 bits (369), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 104/365 (28%), Positives = 178/365 (48%), Gaps = 13/365 (3%) Query: 57 LGAHSFAIKHRIPMLNALSSRVSVNCLKRLCCWEGVHKVHLDHIKSISLDIATPSIGAAA 116 L ++ + + I N +S+ +S N ++RL + V + LD I + S ++ Sbjct: 41 LKSYKSTVFYSITHANCISADISSNAIRRLVEYPEVDYITLDDFCFICA-SPSNSSYPSS 99 Query: 117 VQSKRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGHGTHITGD 176 +++ +GKG+ I ++DTGV+PH DL P+ RI F D +N+ + PYDDNGHGT I+G Sbjct: 100 LRNNTSFSGKGVCIGIVDTGVYPHYDLKYPMPRIDNFVDLINNLEYPYDDNGHGTFISGL 159 Query: 177 AAGNGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMS 236 AG+G SS+G+ +G A + + +K + NG Y S+I+K +E I + ++I+ + Sbjct: 160 IAGSGVSSKGEIKGIAYNSKLYMIKAFEKNGRSYASSILKALEILINDSSEHNIKIICLP 219 Query: 237 FGGPISTSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVTVGAVDDR 296 F L + + A + +V+ +G++ + +I P ++T ++ Sbjct: 220 FETFFENEFLLSLFSKMFKLAADKNIVIVLPSGSNRNTKNSIRGIAALPYSITTSGINTH 279 Query: 297 RTLPQKDDRITFYSSRGPTPCGKI-KPDLVAPGESVISLRAPGSQLA-RNFPYL---RIG 351 + YSS GP GKI KPDL P + SL ++ RN L + Sbjct: 280 EGF-----KAYTYSSSGPL--GKIEKPDLCGPCVDLSSLNCDIKYISQRNNMKLYPRELK 332 Query: 352 KQYFVLSGTSVSTPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRLGLKPNTAGSGEINM 411 Y +GTS + +S A LL+ P L+ + + +K + L + GSG I++ Sbjct: 333 TPYTTYTGTSCAAAYISSILALLLEKNPDLSYKDTISIVKTSCNLLKIPKWQQGSGIIDV 392 Query: 412 RFLLG 416 LL Sbjct: 393 NSLLN 397 >BS_aprE [O] COG1404 Subtilisin-like serine proteases Length = 381 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 122/392 (31%), Positives = 179/392 (45%), Gaps = 51/392 (13%) Query: 28 SGKSSAALPVIVQFKHKITPSRMLALHKHLGAHSFAIKHRIPMLNALSSRVSVNCLKRLC 87 +GKSS IV FK ++ + ++ + +NA ++ + +K L Sbjct: 30 AGKSSTEKKYIVGFKQTMSAMSSAKKKDVISEKGGKVQKQFKYVNAAAATLDEKAVKELK 89 Query: 88 CWEGVHKVHLDHIK---SISLDIATPSIGAAAVQSKRGLTGKGINIAVLDTGV-FPHPDL 143 V V DHI + S+ I A A+ S +G TG + +AV+D+G+ HPDL Sbjct: 90 KDPSVAYVEEDHIAHEYAQSVPYGISQIKAPALHS-QGYTGSNVKVAVIDSGIDSSHPDL 148 Query: 144 VRPVNRIVAFKDFVNHRKRPYDD-NGHGTHITGDAAGNGWSSRGKYRGPAPEAGIVGVKV 202 + FV PY D + HGTH+ G A ++ G +P A + VKV Sbjct: 149 -----NVRGGASFVPSETNPYQDGSSHGTHVAGTIAA--LNNSIGVLGVSPSASLYAVKV 201 Query: 203 LDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMSFGGPISTSCRDDLLCQAVEKAVQAGL 262 LD G G S II GIEW I+ + +++MS GGP ++ L V+KAV +G+ Sbjct: 202 LDSTGSGQYSWIINGIEWAISNN----MDVINMSLGGPTGSTA----LKTVVDKAVSSGI 253 Query: 263 TVVIAAGNSGPG--RKTIESPGISPAAVTVGAVDDRRTLPQKDDRITFYSSRGPTPCGKI 320 V AAGN G T+ P P+ + VGAV+ + R +F SS G Sbjct: 254 VVAAAAGNEGSSGSTSTVGYPAKYPSTIAVGAVNSS------NQRASF-SSAG------- 299 Query: 321 KPDLVAPGESVISLRAPGSQLARNFPYLRIGKQYFVLSGTSVSTPIVSGAAAQLLQSCPS 380 S + + APG + P G Y +GTS++TP V+GAAA +L P+ Sbjct: 300 ---------SELDVMAPGVSIQSTLP----GGTYGAYNGTSMATPHVAGAAALILSKHPT 346 Query: 381 LTPRQVKARLKRNAFRLGLKPNTAGSGEINMR 412 T QV+ RL+ A LG G G IN++ Sbjct: 347 WTNAQVRDRLESTATYLG-NSFYYGKGLINVQ 377 >BH0855 [O] COG1404 Subtilisin-like serine proteases Length = 361 Score = 126 bits (316), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 99/288 (34%), Positives = 142/288 (49%), Gaps = 45/288 (15%) Query: 120 KRGLTGKGINIAVLDTGVFPHPDLVRPVNRIVAFKDFVNHRKRPYDDNGHGTHITGDAAG 179 RG+ G G +AVLDTG+ HPDL RI F++ +D+NGHGTH+ G A Sbjct: 110 NRGIFGNGARVAVLDTGIASHPDL-----RIAGGASFISSEPSYHDNNGHGTHVAGTIAA 164 Query: 180 NGWSSRGKYRGPAPEAGIVGVKVLDINGDGYDSTIIKGIEWCIAEKKRLKLRILSMSFGG 239 ++ G AP A + VKVLD NG G +++ +GIEW I + I++MS G Sbjct: 165 --LNNSIGVLGVAPSADLYAVKVLDRNGSGSLASVAQGIEWAINNN----MHIINMSLGS 218 Query: 240 PISTSCRDDLLCQAVEKAVQAGLTVVIAAGNSGPGRKTIESPGISPAAVTVGAVDDRRTL 299 +S + AV +A AG+ +V AAGN+G R+ + P + V AVD Sbjct: 219 TSGSSTLE----LAVNRANNAGILLVGAAGNTG--RQGVNYPARYSGVMAVAAVD----- 267 Query: 300 PQKDDRITFYSSRGPTPCGKIKPDLVAPGESVISLRAPGSQLARNFPYLRIGKQYFVLSG 359 Q R +F S+ GP I + APG + + G +Y LSG Sbjct: 268 -QNGQRASF-STYGPE----------------IEISAPGVNVNSTY----TGNRYVSLSG 305 Query: 360 TSVSTPIVSGAAAQLLQSCPSLTPRQVKARLKRNAFRLGLKPNTAGSG 407 TS++TP V+G AA + PS T Q++ R+ + A LG P+ G+G Sbjct: 306 TSMATPHVAGVAALVKSRYPSYTNNQIRQRINQTATYLG-SPSLYGNG 352 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,385,983 Number of extensions: 1388672 Number of successful extensions: 2471 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 2378 Number of HSP's successfully gapped: 50 Length of query: 418 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 309 Effective length of database: 25,807,025 Effective search space: 7974370725 Effective search space used: 7974370725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)