BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0197.0003.p (129 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0441 [IQR] COG1028 Dehydrogenases with different specificities... 77 5e-15 L142816 [IQR] COG1028 Dehydrogenases with different specificitie... 75 3e-14 VNG0364G [IQR] COG1028 Dehydrogenases with different specificiti... 74 5e-14 PA1379 [IQR] COG1028 Dehydrogenases with different specificities... 72 3e-13 SMa0326 [IQR] COG1028 Dehydrogenases with different specificitie... 71 3e-13 >TM0441 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 77.4 bits (189), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 45/121 (37%), Positives = 63/121 (52%), Gaps = 5/121 (4%) Query: 9 LKEII-MMSKVWLITGSSRGLGLEIAKAVLNNGDKVVATARRPEQLN----SLVQKYGDQ 63 +KE+ + +V L+TG SRGLG IA+ + G VV +R E+ + L +KYG + Sbjct: 1 MKEVFDLRGRVALVTGGSRGLGFGIAQGLAEAGCSVVVASRNLEEASEAAQKLTEKYGVE 60 Query: 64 VRTTELDVTNXXXXXXXXXXXLDEFGSLDVIVNNAGYANSSPIEETPDEDFRAQIEANLF 123 DV+N ++FG LD +VN AG P EE P ++FR IE NLF Sbjct: 61 TMAFRCDVSNYEEVKKLLEAVKEKFGKLDTVVNAAGINRRHPAEEFPLDEFRQVIEVNLF 120 Query: 124 G 124 G Sbjct: 121 G 121 >L142816 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 270 Score = 74.7 bits (182), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 41/107 (38%), Positives = 60/107 (56%), Gaps = 3/107 (2%) Query: 18 VWLITGSSRGLGLEIAKAVLNNGDKVVATARRPEQLNSLVQKYGDQVRTTELDVTNXXXX 77 + LITG+S G+G + A+ + NG KV ARR ++L L + G V LD+TN Sbjct: 3 IILITGASSGIGYQTAELLAQNGHKVYGAARRMDKLEKL-KSVG--VEPIYLDITNEESC 59 Query: 78 XXXXXXXLDEFGSLDVIVNNAGYANSSPIEETPDEDFRAQIEANLFG 124 +D G +D+++NNAGY + IE+ P E+ + Q E NLFG Sbjct: 60 RKLVDTIIDNEGQIDILINNAGYGSYGAIEDVPLEEAKRQFEVNLFG 106 >VNG0364G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 273 Score = 73.9 bits (180), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 40/110 (36%), Positives = 59/110 (53%), Gaps = 2/110 (1%) Query: 15 MSKVWLITGSSRGLGLEIAKAVLNNGDKVVATARRPEQLNSLVQKYGDQVRTTELDVTNX 74 M KV L+TG S G+G +++ + +VVATAR L +L ++ G T ELDVT Sbjct: 1 MEKVALVTGCSSGVGAATVRSLTDEEWRVVATARDTADLVALAEETG--CETAELDVTKP 58 Query: 75 XXXXXXXXXXLDEFGSLDVIVNNAGYANSSPIEETPDEDFRAQIEANLFG 124 +D+ G LD +VNNAG+A P+E+ P Q + N++G Sbjct: 59 AQCRAVVDDAVDDHGRLDCLVNNAGFAQLGPLEDVPPRKLHRQFDVNVYG 108 >PA1379 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 277 Score = 71.6 bits (174), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 41/108 (37%), Positives = 56/108 (51%) Query: 17 KVWLITGSSRGLGLEIAKAVLNNGDKVVATARRPEQLNSLVQKYGDQVRTTELDVTNXXX 76 K LITG+S G G +A+ L+ G +VV T R E ++L Q LDVT+ Sbjct: 5 KTLLITGASSGFGQALAREALDAGHRVVGTVRSEEARSALEAVAPGQAFGRLLDVTDLAA 64 Query: 77 XXXXXXXXLDEFGSLDVIVNNAGYANSSPIEETPDEDFRAQIEANLFG 124 + G LDV+VN+AGY + +EE+P + R Q E NLFG Sbjct: 65 IEPTVAAIERDIGPLDVLVNSAGYGHEGILEESPLAEMRRQFEVNLFG 112 >SMa0326 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 279 Score = 71.2 bits (173), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 43/108 (39%), Positives = 59/108 (54%), Gaps = 2/108 (1%) Query: 17 KVWLITGSSRGLGLEIAKAVLNNGDKVVATARRPEQLNSLVQKYGDQVRTTELDVTNXXX 76 KVWL+TG S G G IA+ +L G+KVV TAR+ +++ L QK GD + LDV + Sbjct: 7 KVWLVTGCSTGFGRYIAEHLLEVGEKVVVTARKADKIADLEQK-GDAL-ILPLDVIDRDQ 64 Query: 77 XXXXXXXXLDEFGSLDVIVNNAGYANSSPIEETPDEDFRAQIEANLFG 124 FG +DV++NNAG IEET + + R + N FG Sbjct: 65 CQKVVDAAEAHFGRIDVLINNAGIGFFGAIEETDESNARRLFDVNFFG 112 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,455,635 Number of extensions: 263212 Number of successful extensions: 1082 Number of sequences better than 1.0e-05: 150 Number of HSP's gapped: 1068 Number of HSP's successfully gapped: 150 Length of query: 129 Length of database: 38,378,431 Length adjustment: 94 Effective length of query: 35 Effective length of database: 27,537,035 Effective search space: 963796225 Effective search space used: 963796225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 109 (46.6 bits)