BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0196.0002.p (249 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3201 [M] COG3967 Short-chain dehydrogenase involved in D-alan... 74 2e-13 SA2365 [IQR] COG1028 Dehydrogenases with different specificities... 62 7e-10 VC2021 [IQR] COG1028 Dehydrogenases with different specificities... 61 1e-09 RSc0730 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 59 6e-09 SPAC23D3.11 [IQR] COG1028 Dehydrogenases with different specific... 59 8e-09 >RSc3201 [M] COG3967 Short-chain dehydrogenase involved in D-alanine esterification of lipoteichoic acid and wall teichoic acid (D-alanine transfer protein) Length = 179 Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 47/154 (30%), Positives = 76/154 (49%), Gaps = 9/154 (5%) Query: 72 LIEEHPDLNVLINNAGIIQPDNAGGVIDEDVLVSTVKTNLLGPIRMTSALIEHLKSKEEA 131 L EHP LN++INNAGI + + L V N PI + + L+ L+ +++A Sbjct: 5 LKAEHPALNMVINNAGIQAQHDFTSNPSMESLDQEVAINFTAPIHLIAELLPTLRRQDKA 64 Query: 132 VVINTTSILGFMPLXXXXXXXXXXXXLHTYTLSQRYMLKDTSVKVLEIVPPWVQSN-NNE 190 +IN +S L F P+ +H++TLS R+ LK T ++V+E+ PP V ++ Sbjct: 65 YIINVSSGLAFSPMADVPVYCATKAAIHSFTLSLRHQLKATGIRVIEVAPPIVDTSLGGN 124 Query: 191 PRAMPLAS--------FIEATIKVLGTDTDEVVV 216 R+ A+ F + L D DE++V Sbjct: 125 TRSAGTANRMIITADEFATEALAQLEADKDEILV 158 >SA2365 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 234 Score = 62.0 bits (149), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 42/122 (34%), Positives = 66/122 (54%), Gaps = 1/122 (0%) Query: 20 ALAEALHKLGNKVIISGRSKERLEETIQANPGISAVELNVQDPASIQAAAKQLIEEHPDL 79 A AEAL LG KV I R+ R ++ Q G+ V+L+V S++ A + E+ L Sbjct: 17 ASAEALKALGYKVYIGSRNDVRGQQASQ-KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRL 75 Query: 80 NVLINNAGIIQPDNAGGVIDEDVLVSTVKTNLLGPIRMTSALIEHLKSKEEAVVINTTSI 139 ++LINNAGI +A + + +TN+ G +RM + + L+ E+ VV+N +S Sbjct: 76 DILINNAGISGQFSAPSKLTPRDVEEVYQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSG 135 Query: 140 LG 141 LG Sbjct: 136 LG 137 >VC2021 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 248 Score = 61.2 bits (147), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 49/187 (26%), Positives = 91/187 (48%), Gaps = 11/187 (5%) Query: 19 RALAEALHKLGNKVIISGRSK---ERLEETIQANPGISAVELNVQDPASIQAAAKQLIEE 75 +A+AE L + G KVI + S+ + + + + N + LNV +P SI+A K + +E Sbjct: 23 KAIAELLAERGAKVIGTATSESGAQAISDYLGDNG--KGMALNVTNPESIEAVLKAITDE 80 Query: 76 HPDLNVLINNAGIIQPDNAGGVIDEDVLVSTVKTNLLGPIRMTSALIEHLKSKEEAVVIN 135 +++L+NNAGI + DN + E+ ++TNL R++ A++ + K + +IN Sbjct: 81 FGGVDILVNNAGITR-DNLLMRMKEEEWSDIMETNLTSIFRLSKAVLRGMMKKRQGRIIN 139 Query: 136 TTSILGFMPLXXXXXXXXXXXXLHTYTLSQRYMLKDTSVKVLEIVPPWVQSN-----NNE 190 S++G M + +T S + V V + P +++++ N+E Sbjct: 140 VGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGFIETDMTKALNDE 199 Query: 191 PRAMPLA 197 R LA Sbjct: 200 QRTATLA 206 >RSc0730 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 262 Score = 58.9 bits (141), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 48/167 (28%), Positives = 88/167 (52%), Gaps = 9/167 (5%) Query: 20 ALAEALHKLGNKVIISGRSKERLEETIQANPGISAVE--LNVQDPASIQAAAKQLIEEHP 77 A+A + G++VI +GR ++RL+ ++A G + + L+VQD A++ A L E Sbjct: 27 AVARRFVREGHRVIAAGRRQDRLD-ALKAELGDALLPFLLDVQDAAAVAALPGALPEGWR 85 Query: 78 DLNVLINNAGI---IQPDNAGGVIDEDVLVSTVKTNLLGPIRMTSALIEHLKSKEEAVVI 134 +++VL+NNAG+ ++P + + D D+++ T N+ G + +T AL+ + ++ VI Sbjct: 86 EVDVLVNNAGLALGLEPAHRASLSDWDLMIGT---NVTGLVHVTRALLPGMVARNRGHVI 142 Query: 135 NTTSILGFMPLXXXXXXXXXXXXLHTYTLSQRYMLKDTSVKVLEIVP 181 N SI G P + ++L+ R L T V+V + P Sbjct: 143 NLGSIAGTYPYPGGNVYGGTKAFVRQFSLNLRADLTGTRVRVSNVEP 189 >SPAC23D3.11 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 296 Score = 58.5 bits (140), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 49/187 (26%), Positives = 92/187 (49%), Gaps = 10/187 (5%) Query: 20 ALAEALHKLGNKVIISGRSKERLEETIQANPGISAVELNVQDPASIQAAAKQLIE-EHPD 78 ALA H+ G +V+ + R ER++ +A G+ ++L+V D S++ +++ + + Sbjct: 20 ALALKFHQEGFQVLATARQVERMDNLTKA--GLQTLKLDVTDEDSVREVEQEVRKFTNGS 77 Query: 79 LNVLINNAGIIQPDNAGGV-IDEDVLVSTVKTNLLGPIRMTSALIEHLKSKEEAVVINTT 137 L+ LINNAG P +A + +D + + + N G IRM A +H + + ++N Sbjct: 78 LHYLINNAG--APCSAPAIDLDIEDVSKVMDVNFYGVIRMNKAF-QHQLIRAKGTIVNVN 134 Query: 138 SILGFMPLXXXXXXXXXXXXLHTYTLSQRYMLKDTSVKVLEIVPPWVQSNNNEPRAMPLA 197 S++ ++P L Y+ + R L V+V I+ VQ+ + ++ PL Sbjct: 135 SLVSYVPFAFNAAYNASKAALLAYSNTLRIELAPFGVQVTSIMTGGVQT---KIQSKPLG 191 Query: 198 SFIEATI 204 + EA I Sbjct: 192 TMTEAAI 198 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.131 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,303,113 Number of extensions: 582969 Number of successful extensions: 1321 Number of sequences better than 1.0e-05: 48 Number of HSP's gapped: 1376 Number of HSP's successfully gapped: 48 Length of query: 249 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 145 Effective length of database: 26,383,695 Effective search space: 3825635775 Effective search space used: 3825635775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 114 (48.5 bits)