BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0027.p (210 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0264 [K] COG5662 Predicted transmembrane transcriptional regul... 120 2e-27 >BH0264 [K] COG5662 Predicted transmembrane transcriptional regulator (anti-sigma factor) Length = 213 Score = 120 bits (301), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 62/195 (31%), Positives = 104/195 (53%), Gaps = 3/195 (1%) Query: 14 SMMHDYLDDDLPELQQRELKEHLLSCTDCRAKFKELEQTDMLMFSLMHQNPVASEDLVGR 73 +++ Y+D + +++EL EHL +C C E+ + + S H A E Sbjct: 9 TLIDKYIDGEATAEERQELNEHLETCDACFEYMLEVRKVVAFVQSASHVQ--APEGFTEN 66 Query: 74 IMNSLPHVKKERAFITWIKRHPALTAASVFILVMLMSSVTFWNQD-RQLVVRGSDLEQVV 132 +M +LP K+ F W++R+P AA+VF+L+M S + W+ D + V G+ + Sbjct: 67 VMKNLPKRKQTNRFKVWMRRYPLAVAAAVFVLLMSTSLFSMWSSDGEHVTVTGTGNVSID 126 Query: 133 IKGDTVIVPSGKTISGDLTVENGKTQVYGEVNGNVTVIDGSLYQASTAHISGQVKSIDQA 192 + VIVP G+ I GDL V NG+ + GEV GNV +++ S Y AS +SG++ ++Q Sbjct: 127 QESGRVIVPEGEVIQGDLVVRNGELIIEGEVQGNVLLVNSSQYLASPGSVSGEIDEVNQI 186 Query: 193 VSWLWYKVTNMFSEV 207 + W+WY ++V Sbjct: 187 LDWIWYHTKKFLTKV 201 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,791,340 Number of extensions: 558681 Number of successful extensions: 1703 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 1701 Number of HSP's successfully gapped: 1 Length of query: 210 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 108 Effective length of database: 26,614,363 Effective search space: 2874351204 Effective search space used: 2874351204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)