BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0017.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl249 [R] COG3608 Predicted deacylase 253 3e-67 AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of... 253 3e-67 AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 253 3e-67 AGl2475 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases 253 3e-67 AGl2433 [I] COG0204 1-acyl-sn-glycerol-3-phosphate acyltransferase 253 3e-67 >AGl249 [R] COG3608 Predicted deacylase Length = 320 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 128/293 (43%), Positives = 183/293 (62%) Query: 6 NLIQKQKELAELIDRFSPGEGVHATDIPSLYCIRSSKITEPIYRVHEPALCIVAQGKKEV 65 ++++ + + I R +P +G T +P L IR S+ T P+ V+EP LC+VAQG+K+ Sbjct: 14 SIMKSEHTIEATIARHAPHDGTFETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQA 73 Query: 66 MLTEERYQYGPSDYLVVSVDLPVSGQIIEALSDVPYLCFRLDFKPAQIVEILEESELRAV 125 +L + + Y PS YLV SVDLPV G +IEA D PYL +LD ++ ++ Sbjct: 74 VLGQTSFVYDPSSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQH 133 Query: 126 QPADSRRGLFISQTSTSLLDAAIRLVRLLETPEDIKALAPMYIREILYRILKGQQGDVLK 185 P + GL +++ ++DAA RL+ LL+TP DI AL+P+ REILYR++ G G L+ Sbjct: 134 APINGPIGLLLNKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALR 193 Query: 186 QIAVEGSSAYRIKEVIEQIKQNYAQPLRIEELAELANMSPSSLHRHFKEVTAMSPLQYQK 245 QI G+ +I I I++++ + +E AE+A MS S+ H HFK VT MSPL+++ Sbjct: 194 QIVQAGAHVNQIARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRT 253 Query: 246 RIRLQEARRLLLSETADAAEAAFQVGYDSPSHFSREYARMFGLPPIADMKRLR 298 ++RLQEARRL+LSE DAA A FQVGY SPS FSREY R+FG+ P LR Sbjct: 254 QLRLQEARRLMLSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAKHANALR 306 >AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 320 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 128/293 (43%), Positives = 183/293 (62%) Query: 6 NLIQKQKELAELIDRFSPGEGVHATDIPSLYCIRSSKITEPIYRVHEPALCIVAQGKKEV 65 ++++ + + I R +P +G T +P L IR S+ T P+ V+EP LC+VAQG+K+ Sbjct: 14 SIMKSEHTIEATIARHAPHDGTFETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQA 73 Query: 66 MLTEERYQYGPSDYLVVSVDLPVSGQIIEALSDVPYLCFRLDFKPAQIVEILEESELRAV 125 +L + + Y PS YLV SVDLPV G +IEA D PYL +LD ++ ++ Sbjct: 74 VLGQTSFVYDPSSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQH 133 Query: 126 QPADSRRGLFISQTSTSLLDAAIRLVRLLETPEDIKALAPMYIREILYRILKGQQGDVLK 185 P + GL +++ ++DAA RL+ LL+TP DI AL+P+ REILYR++ G G L+ Sbjct: 134 APINGPIGLLLNKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALR 193 Query: 186 QIAVEGSSAYRIKEVIEQIKQNYAQPLRIEELAELANMSPSSLHRHFKEVTAMSPLQYQK 245 QI G+ +I I I++++ + +E AE+A MS S+ H HFK VT MSPL+++ Sbjct: 194 QIVQAGAHVNQIARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRT 253 Query: 246 RIRLQEARRLLLSETADAAEAAFQVGYDSPSHFSREYARMFGLPPIADMKRLR 298 ++RLQEARRL+LSE DAA A FQVGY SPS FSREY R+FG+ P LR Sbjct: 254 QLRLQEARRLMLSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAKHANALR 306 >AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 320 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 128/293 (43%), Positives = 183/293 (62%) Query: 6 NLIQKQKELAELIDRFSPGEGVHATDIPSLYCIRSSKITEPIYRVHEPALCIVAQGKKEV 65 ++++ + + I R +P +G T +P L IR S+ T P+ V+EP LC+VAQG+K+ Sbjct: 14 SIMKSEHTIEATIARHAPHDGTFETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQA 73 Query: 66 MLTEERYQYGPSDYLVVSVDLPVSGQIIEALSDVPYLCFRLDFKPAQIVEILEESELRAV 125 +L + + Y PS YLV SVDLPV G +IEA D PYL +LD ++ ++ Sbjct: 74 VLGQTSFVYDPSSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQH 133 Query: 126 QPADSRRGLFISQTSTSLLDAAIRLVRLLETPEDIKALAPMYIREILYRILKGQQGDVLK 185 P + GL +++ ++DAA RL+ LL+TP DI AL+P+ REILYR++ G G L+ Sbjct: 134 APINGPIGLLLNKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALR 193 Query: 186 QIAVEGSSAYRIKEVIEQIKQNYAQPLRIEELAELANMSPSSLHRHFKEVTAMSPLQYQK 245 QI G+ +I I I++++ + +E AE+A MS S+ H HFK VT MSPL+++ Sbjct: 194 QIVQAGAHVNQIARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRT 253 Query: 246 RIRLQEARRLLLSETADAAEAAFQVGYDSPSHFSREYARMFGLPPIADMKRLR 298 ++RLQEARRL+LSE DAA A FQVGY SPS FSREY R+FG+ P LR Sbjct: 254 QLRLQEARRLMLSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAKHANALR 306 >AGl2475 [J] COG0008 Glutamyl- and glutaminyl-tRNA synthetases Length = 252 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 0/293 (0%), Positives = 0/293 (0%) Query: 6 NLIQKQKELAELIDRFSPGEGVHATDIPSLYCIRSSKITEPIYRVHEPALCIVAQGKKEV 65 + E V + P R+ + Sbjct: 14 AERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARS 73 Query: 66 MLTEERYQYGPSDYLVVSVDLPVSGQIIEALSDVPYLCFRLDFKPAQIVEILEESELRAV 125 L + ++ L + + + + ++ Sbjct: 74 ALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDN 133 Query: 126 QPADSRRGLFISQTSTSLLDAAIRLVRLLETPEDIKALAPMYIREILYRILKGQQGDVLK 185 + + + D + + DI + A + Sbjct: 134 WLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAI 193 Query: 186 QIAVEGSSAYRIKEVIEQIKQNYAQPLRIEELAELANMSPSSLHRHFKEVTAMSPLQYQK 245 Sbjct: 194 253 Query: 246 RIRLQEARRLLLSETADAAEAAFQVGYDSPSHFSREYARMFGLPPIADMKRLR 298 Sbjct: 254 306 >AGl2433 [I] COG0204 1-acyl-sn-glycerol-3-phosphate acyltransferase Length = 320 Score = 253 bits (646), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 128/293 (43%), Positives = 183/293 (62%) Query: 6 NLIQKQKELAELIDRFSPGEGVHATDIPSLYCIRSSKITEPIYRVHEPALCIVAQGKKEV 65 ++++ + + I R +P +G T +P L IR S+ T P+ V+EP LC+VAQG+K+ Sbjct: 14 SIMKSEHTIEATIARHAPHDGTFETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQA 73 Query: 66 MLTEERYQYGPSDYLVVSVDLPVSGQIIEALSDVPYLCFRLDFKPAQIVEILEESELRAV 125 +L + + Y PS YLV SVDLPV G +IEA D PYL +LD ++ ++ Sbjct: 74 VLGQTSFVYDPSSYLVASVDLPVMGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQH 133 Query: 126 QPADSRRGLFISQTSTSLLDAAIRLVRLLETPEDIKALAPMYIREILYRILKGQQGDVLK 185 P + GL +++ ++DAA RL+ LL+TP DI AL+P+ REILYR++ G G L+ Sbjct: 134 APINGPIGLLLNKVDEGIIDAASRLLALLDTPRDIDALSPLAKREILYRLITGVGGAALR 193 Query: 186 QIAVEGSSAYRIKEVIEQIKQNYAQPLRIEELAELANMSPSSLHRHFKEVTAMSPLQYQK 245 QI G+ +I I I++++ + +E AE+A MS S+ H HFK VT MSPL+++ Sbjct: 194 QIVQAGAHVNQIARSIVWIRKHFRETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRT 253 Query: 246 RIRLQEARRLLLSETADAAEAAFQVGYDSPSHFSREYARMFGLPPIADMKRLR 298 ++RLQEARRL+LSE DAA A FQVGY SPS FSREY R+FG+ P LR Sbjct: 254 QLRLQEARRLMLSEGLDAATAGFQVGYASPSQFSREYGRVFGMSPAKHANALR 306 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,032,370 Number of extensions: 813391 Number of successful extensions: 13652 Number of sequences better than 1.0e-05: 112 Number of HSP's gapped: 13623 Number of HSP's successfully gapped: 112 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)