BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0015.p (280 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0317 [ET] COG0834 ABC-type amino acid transport/signal transd... 196 4e-50 SP0148 [ET] COG0834 ABC-type amino acid transport/signal transdu... 173 3e-43 SP0620 [ET] COG0834 ABC-type amino acid transport/signal transdu... 169 4e-42 L162009 [ET] COG0834 ABC-type amino acid transport/signal transd... 167 1e-41 L121253 [ET] COG0834 ABC-type amino acid transport/signal transd... 129 5e-30 >SPy0317 [ET] COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain Length = 280 Score = 196 bits (497), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 110/280 (39%), Positives = 161/280 (57%), Gaps = 9/280 (3%) Query: 1 MKKRFLPGVFILLLG-VIIAGCGNKSEAGSGAQNDAGAGSKTIVAATSGVSNPFSYDKDG 59 +KK++L I L +++A CG+ A SG Q SK ++ AT G + PFSY+K G Sbjct: 2 IKKQYLGRATIALASTLVLAACGSSKTAESGNQ----GSSKEVLFATVGTTAPFSYEKGG 57 Query: 60 KLTGYDVEVMKAIFEGLPEYKLEVQAIEFEGILTGLDNGRFQLGANNFGSNAERRSKYNF 119 +LTGYD+EV KA+F+G +YK+ + E+ I TGLD+G++Q+G NN ER +KY F Sbjct: 58 QLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGGNNISFTKERSAKYLF 117 Query: 120 SLPIIENANVFVVRKDDNTLKSVEDLKGYKAVTEVGNSGATLLENYNADHPDAKAEIMYT 179 S PI +V VV KD + +KS +D++G+ G + LE++N H D + +T Sbjct: 118 SYPIGSTPSVLVVPKDSD-IKSFDDIQGHTTQVVQGTTSVAQLEDFNKKHSDNPVTLKFT 176 Query: 180 DENFVKQFEGIEAGRYDVRIISRVSAEKAIKEHGFTNLKVVPFSTENSDPGSYILFSKSA 239 +EN + + G+ D +I + IK G NLK + T P Y +FS+ Sbjct: 177 NENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIEL-TSTEQPFIYFIFSQDQ 235 Query: 240 DSTLLDAVNKRIKEIYNDGTLLKISEEQLGGDYLPK-KEL 278 + L VNKRIKE+ DGTL K+++E LGGDY+P KEL Sbjct: 236 EK-LQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKEL 274 >SP0148 [ET] COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain Length = 276 Score = 173 bits (438), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 90/248 (36%), Positives = 142/248 (57%), Gaps = 2/248 (0%) Query: 27 AGSGAQNDAGAGSKTIVAATSGVSNPFSYDKDGKLTGYDVEVMKAIFEGLPEYKLEVQAI 86 +G + A K I+ AT+G PF Y+++G+LTGY++EV++AIF+ +Y ++ + Sbjct: 25 SGGAKKEGEAASKKEIIVATNGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKT 84 Query: 87 EFEGILTGLDNGRFQLGANNFGSNAERRSKYNFSLPIIENANVFVVRKDDNTLKSVEDLK 146 E+ G+ GLD R+ + NN ER KY ++ PI +N NV VV+KDD+++KS++D+ Sbjct: 85 EWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIG 144 Query: 147 GYKAVTEVGNSGATLLENYNADHPDAKAEIMYTDENFVKQFEGIEAGRYDVRIISRVSAE 206 G + A LE YNA+H D + YT + + + G++D +I ++ E Sbjct: 145 GKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKADLQQIMVRLSDGQFDYKIFDKIGVE 204 Query: 207 KAIKEHGFTNLKVVPFSTENSDPGSYILFSKSADSTLLDAVNKRIKEIYNDGTLLKISEE 266 IK G NLKV+ ++ P Y L ++ D L V+KRIKE+Y DGTL K+S++ Sbjct: 205 TVIKNQGLDNLKVIELPSDQQ-PYVYPLLAQGQDE-LKSFVDKRIKELYKDGTLEKLSKQ 262 Query: 267 QLGGDYLP 274 G YLP Sbjct: 263 FFGDTYLP 270 >SP0620 [ET] COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain Length = 266 Score = 169 bits (429), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 101/273 (36%), Positives = 149/273 (54%), Gaps = 12/273 (4%) Query: 10 FILLLGVI--IAGCGNKSEAGSGAQNDAGAGSKTIVAATSGVSNPFSYDKDGKLTGYDVE 67 F LLL ++ + C N+ A + KTIV AT+G PF Y+ G LTG+D+E Sbjct: 4 FSLLLAILPFLVACENQ------ATPKETSAQKTIVLATAGDVPPFDYEDKGNLTGFDIE 57 Query: 68 VMKAIFEGLPEYKLEVQAIEFEGILTGLDNGRFQLGANNFGSNAERRSKYNFSLPIIENA 127 V+KA+ E L +Y+++ Q +E I GLD+G +Q ANN ER KY +SLPI N Sbjct: 58 VLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKERAEKYLYSLPISNNP 117 Query: 128 NVFVVRKDDNTLKSVEDLKGYKAVTEVGNSGATLLENYNADHPDAKAEIMYTDENFVKQF 187 V V K N L S++ + G + G S A + N+N H D A I ++ E+ K+ Sbjct: 118 LVLVSNK-KNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTDNPATINFSGEDIGKRI 176 Query: 188 EGIEAGRYDVRIISRVSAEKAIKEHGFTNLKVVPFSTENSDPGSYILFSKSADSTLLDAV 247 + G +D + +VS +K IK+ G +L VV + +S P +YI+FS S + Sbjct: 177 LDLANGEFDFLVFDKVSVQKIIKDRGL-DLSVVDLPSADS-PSNYIIFS-SDQKEFKEQF 233 Query: 248 NKRIKEIYNDGTLLKISEEQLGGDYLPKKELME 280 +K +KE+Y DGTL K+S LGG YLP + ++ Sbjct: 234 DKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ 266 >L162009 [ET] COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain Length = 281 Score = 167 bits (424), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 91/248 (36%), Positives = 148/248 (59%), Gaps = 10/248 (4%) Query: 41 TIVAATSGVSNPFSY-DKDGKLTGYDVEVMKAIFEGLPEYKLEVQAIEFEGILTGLDNGR 99 ++V AT G + PF+Y D G LTGYD+EV +A+F+ LP+YK++ Q +F GI G+D+GR Sbjct: 36 SVVIATDGATKPFTYSDSQGNLTGYDIEVARAVFKKLPQYKVKFQVTDFSGISPGIDSGR 95 Query: 100 FQLGANNFGSNAERRSKYNFSLPIIENANVFVVRKDDNTLKSVEDLKGYKAVTEVGNSGA 159 +Q+GAN+FG R KY FS P+ ++ N +V+ T K++ DL G + ++ Sbjct: 96 YQMGANDFGWEKSRAEKYYFSSPLSKSNNAVLVK--SGTYKTLADLAGKSTIGNPASNYT 153 Query: 160 TLLENYNADHPDAKAEIMYTDE--NFVKQFEGIEAGRYDVRIISRVSAEKAIKEHGFTNL 217 ++++N +PD + +I Y+ + + +F +E+G+ D + ++S E A+KE GF NL Sbjct: 154 KSIQDWNKANPDKEIKISYSADSTSINTRFTQVESGKIDFMLYDKISLESAVKEQGFDNL 213 Query: 218 KVVPFSTENSDPGS----YILFSK-SADSTLLDAVNKRIKEIYNDGTLLKISEEQLGGDY 272 K+ S + D Y LF K S L VN + ++ DG+L KIS++ LGGD+ Sbjct: 214 KIEDISMDTGDAEHDGYEYFLFGKDSQGKKLQKDVNGVLAKMQADGSLAKISKKYLGGDF 273 Query: 273 LPKKELME 280 +PK E+ + Sbjct: 274 VPKAEMFK 281 >L121253 [ET] COG0834 ABC-type amino acid transport/signal transduction systems, periplasmic component/domain Length = 280 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 74/238 (31%), Positives = 118/238 (49%), Gaps = 6/238 (2%) Query: 41 TIVAATSGVSNPFSYDKDGKLTGYDVEVMKAIFEGLPEYKLEVQAIEFEGILTGLDNGRF 100 T+ ATS S P+ Y ++GK+TG++ +++KA + + +YK + + E IL LD GR Sbjct: 39 TVTVATSAASVPYEYSENGKMTGFEYDILKAADKDMKDYKFDFKVYEDSAILAALDGGRA 98 Query: 101 QLGANNFGSNAERRSKYNFSLPIIENANVFVVRKDDNTLKSVEDLKGYKAVTEVGNSGAT 160 Q+ ANNFG R KY FS P+ + + +N K + L G G + Sbjct: 99 QIAANNFGKTKARSEKYLFSYPVRQGIDAIFSTSKENITK-ISQLAGKTTEIPAGTNYGD 157 Query: 161 LLENYNADHPDAKAEIMYTDENFVKQFEGIEAGRYDVRIISRVSAEKAIKEHGFTNL-KV 219 + E++N +HPD K + Y+ + I +G+ D S+ AE IKEH T L Sbjct: 158 MFESWNKNHPDKKINVKYSQRPLADRLSAISSGQIDFLFASKAPAENLIKEHAITGLVDT 217 Query: 220 VPFSTENS---DPGSYILFSKSADSTLLDAVNKRIKEIYNDGTLLKISEEQLGGDYLP 274 VP + Y + S S L +N +K++Y++GTL K+SE+ ++P Sbjct: 218 VPTDLDKQPEFKTYDYFILDNS-QSKLQKDLNVELKKLYDNGTLKKLSEQYFHDSHIP 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.134 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,277,659 Number of extensions: 887467 Number of successful extensions: 2265 Number of sequences better than 1.0e-05: 88 Number of HSP's gapped: 2188 Number of HSP's successfully gapped: 88 Length of query: 280 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 175 Effective length of database: 26,268,361 Effective search space: 4596963175 Effective search space used: 4596963175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)