BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0014.p (444 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMB1185 [M] COG2843 Putative enzyme of poly-gamma-glutamate bios... 52 2e-06 NMB1147 [M] COG2843 Putative enzyme of poly-gamma-glutamate bios... 52 2e-06 >NMB1185 [M] COG2843 Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation) Length = 252 Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 7/93 (7%), Positives = 19/93 (20%), Gaps = 10/93 (10%) Query: 231 QDQQEIFRTIRDARERSDFVFVSLHT-HEG--------ENENWYSDYPAEFIETFARGAV 281 + R + + + + + +D A + Sbjct: 39 GCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPV-QALDLI 97 Query: 282 DAGASCVFGHGAHFTRGVELYQGQPIFYNLGSL 314 A AS + + + P + Sbjct: 98 FANASLQWLPDHYELFPHLVSLLNPQGVLAVQM 130 >NMB1147 [M] COG2843 Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation) Length = 252 Score = 52.0 bits (123), Expect = 2e-06, Method: Compositional matrix adjust. Identities = 7/93 (7%), Positives = 19/93 (20%), Gaps = 10/93 (10%) Query: 231 QDQQEIFRTIRDARERSDFVFVSLHT-HEG--------ENENWYSDYPAEFIETFARGAV 281 + R + + + + + +D A + Sbjct: 39 GCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPV-QALDLI 97 Query: 282 DAGASCVFGHGAHFTRGVELYQGQPIFYNLGSL 314 A AS + + + P + Sbjct: 98 FANASLQWLPDHYELFPHLVSLLNPQGVLAVQM 130 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,750,358 Number of extensions: 1503374 Number of successful extensions: 12577 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 12577 Number of HSP's successfully gapped: 2 Length of query: 444 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 334 Effective length of database: 25,691,691 Effective search space: 8581024794 Effective search space used: 8581024794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)