BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0002.p (335 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfA [P] COG1055 Na+/H+ antiporter NhaD and related arsenite ... 456 e-128 BH2999 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 415 e-116 SAP017 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 400 e-111 SA1592 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite p... 389 e-108 arsB [P] COG1055 Na+/H+ antiporter NhaD and related arsenite per... 330 2e-90 >BS_ydfA [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 51 Score = 456 bits (1174), Expect = e-128, Method: Compositional matrix adjust. Identities = 0/289 (0%), Positives = 0/289 (0%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 Sbjct: 119 178 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 Sbjct: 179 238 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 Sbjct: 239 298 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 Sbjct: 299 358 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 Sbjct: 359 407 >BH2999 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 436 Score = 415 bits (1067), Expect = e-116, Method: Compositional matrix adjust. Identities = 207/293 (70%), Positives = 246/293 (83%), Gaps = 4/293 (1%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVRAL F E V PF++A GFIADTTSLPL+VSNLVNIVSADFF + F+EY Sbjct: 119 LILTPIVLAMVRALKFKEAMVLPFVMASGFIADTTSLPLIVSNLVNIVSADFFNIGFVEY 178 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A RM++P LF+L ASI+VLYL+FR+ +P+ +D SQLK P EAIKDK MFKLSW +LAVLL Sbjct: 179 ATRMVVPNLFALAASIVVLYLYFRKDMPKTYDLSQLKKPHEAIKDKAMFKLSWVILAVLL 238 Query: 143 AGYFVSEFLNIPVSAVAGVVA-IFFLIMARKSPNVHAME---IVKGAPWAIVFFSIGMYV 198 GYFVSEF++IPVS VA +A +F+LI R S A+ I+KGAPWAIV FSIGMYV Sbjct: 239 IGYFVSEFVHIPVSFVAMPIAFVFYLIFRRASGKNKALNTTGIIKGAPWAIVVFSIGMYV 298 Query: 199 VVYGLRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDAT 258 VVYGLRN GLT++LAD+I+ A QGLFA ++ MGFIAA++SS+MNN+PTVMI+ALAI T Sbjct: 299 VVYGLRNVGLTNILADIIELAAAQGLFAGTMAMGFIAAILSSLMNNMPTVMINALAIADT 358 Query: 259 HTSGLIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 +T G+++EALIYANVIGSDLGPK TPIGSLATLLWLHVLS KG+KI+WG YFK Sbjct: 359 NTMGIMREALIYANVIGSDLGPKITPIGSLATLLWLHVLSKKGMKITWGYYFK 411 >SAP017 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 429 Score = 400 bits (1028), Expect = e-111, Method: Compositional matrix adjust. Identities = 194/289 (67%), Positives = 245/289 (84%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMVR L F++K +FPFIIA GFIADTTSLPL+VSNLVNIVSAD+F + F+EY Sbjct: 117 LILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSNLVNIVSADYFDIGFIEY 176 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 RM+IP +FSL ASILVL+L+FR+SIP+ FD+ L +P+ AIKD ++FKLSW VLAVLL Sbjct: 177 FSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKSAIKDSKLFKLSWIVLAVLL 236 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 GY VSEF+ IPVS +AG++A F+I+ARKS VH +++KGAPW IV FSIGMY+VV+G Sbjct: 237 VGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQVIKGAPWNIVVFSIGMYLVVFG 296 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 L+N G+T VL DV+ +++ GLF++ +GMGFIAA +SS+MNN+PTV+IDA+AI + +G Sbjct: 297 LKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLSSIMNNMPTVLIDAIAIGQSSATG 356 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 ++KE ++YANVIGSDLGPK TPIGSLATLLWLHVL+ KG+KISWGTYFK Sbjct: 357 ILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVLTQKGVKISWGTYFK 405 >SA1592 [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 430 Score = 389 bits (1000), Expect = e-108, Method: Compositional matrix adjust. Identities = 182/289 (62%), Positives = 240/289 (83%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIVLAMV+ + F ++ +FPFIIA GFIADTTSLPL+VSNLVNI+SAD+F V F+ Y Sbjct: 118 LILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSNLVNIISADYFHVGFVRY 177 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 RM+IP LFSL ASI+VL+L+FR++IP+ FD + +K+P++AI D ++FK+SW VL +LL Sbjct: 178 FSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKDAINDLKLFKISWIVLVILL 237 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 GY +SEF IPVS G++A FL++ARKS V+ +++KGAPW IV FSIGMY+VV+G Sbjct: 238 FGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQVIKGAPWNIVLFSIGMYIVVFG 297 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 LRNAG+T +LA +++ +++ GLF+ +GMGFI+A +SS+MNN+PTV+IDA+AI ++ G Sbjct: 298 LRNAGITLILAKILEYISNYGLFSTILGMGFISAFLSSIMNNMPTVLIDAIAIGQSNVHG 357 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 ++KE LIYANVIGSDLGPK TPIGSLATLLWLHVL+ K +KISWGTYFK Sbjct: 358 MLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVLTQKDVKISWGTYFK 406 >arsB [P] COG1055 Na+/H+ antiporter NhaD and related arsenite permeases Length = 436 Score = 330 bits (845), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 167/289 (57%), Positives = 225/289 (77%) Query: 23 LILTPIVLAMVRALDFDEKKVFPFIIAGGFIADTTSLPLVVSNLVNIVSADFFGVTFMEY 82 LILTPIV+AM+ AL F + F++A GFIADT SLPL+VSNLVNIVSADFFG+ F EY Sbjct: 123 LILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREY 182 Query: 83 AGRMLIPTLFSLGASILVLYLFFRRSIPRNFDSSQLKNPEEAIKDKQMFKLSWFVLAVLL 142 A M+ + ++ A++++L+L+FR+ IP+N+D + LK+P EAIKD FK W VL +LL Sbjct: 183 ASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLL 242 Query: 143 AGYFVSEFLNIPVSAVAGVVAIFFLIMARKSPNVHAMEIVKGAPWAIVFFSIGMYVVVYG 202 G+FV E L IPVSA+A V A+ ++A++ ++ ++++GAPW IV FS+GMY+VVYG Sbjct: 243 VGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYG 302 Query: 203 LRNAGLTDVLADVIQAVADQGLFAASVGMGFIAAVISSVMNNLPTVMIDALAIDATHTSG 262 LRNAGLT+ L+ V+ +AD GL+AA++G GF+ A +SS+MNN+PTV++ AL+ID + SG Sbjct: 303 LRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASG 362 Query: 263 LIKEALIYANVIGSDLGPKFTPIGSLATLLWLHVLSTKGLKISWGTYFK 311 +IKEA++YANVIG DLGPK TPIGSLATLLWLHVLS K + ISWG YF+ Sbjct: 363 VIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFR 411 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,802,724 Number of extensions: 732483 Number of successful extensions: 2569 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 2552 Number of HSP's successfully gapped: 20 Length of query: 335 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 228 Effective length of database: 26,037,693 Effective search space: 5936594004 Effective search space used: 5936594004 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)