BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0091.p (230 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr1404 [E] COG1045 Serine acetyltransferase 247 1e-65 all4037 [E] COG1045 Serine acetyltransferase 243 1e-64 PA3816 [E] COG1045 Serine acetyltransferase 235 3e-62 MA2721_1 [E] COG1045 Serine acetyltransferase 223 2e-58 RSc1162 [E] COG1045 Serine acetyltransferase 209 2e-54 >alr1404 [E] COG1045 Serine acetyltransferase Length = 359 Score = 247 bits (630), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 14/219 (6%), Positives = 33/219 (15%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 + AR+ + A K ++ Sbjct: 28 KMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDL 87 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 ++ H +L + T + D +++ + R Sbjct: 88 PTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQC 147 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 N + + + S Sbjct: 148 KNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDW 207 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQLGTEDKQKKEQRRL 219 S + + Q R Sbjct: 208 VRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVRE 246 >all4037 [E] COG1045 Serine acetyltransferase Length = 253 Score = 243 bits (621), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 114/206 (55%), Positives = 154/206 (74%), Gaps = 1/206 (0%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 M +++D R +F+ DPAAR+ EV+F Y GL A+ HR+A+ Y+ + R++S ++ Sbjct: 1 MLSILRADFRIIFERDPAARNLLEVLFCYPGLQALLFHRVANWLYRLGLPFIPRLISHIA 60 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 RF+TG+EIHPGATIG +FIDHGMG+VIGET +GD +IYQGVTLGGTGKE GKRHPT+ Sbjct: 61 RFLTGIEIHPGATIGQGVFIDHGMGVVIGETAIVGDYALIYQGVTLGGTGKESGKRHPTL 120 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 G NVV+G+GAKVLG+ QIG+N IG+ +VVLR+VP+N TVVG PGR+V R+G RV D L+ Sbjct: 121 GENVVVGAGAKVLGNLQIGNNVRIGAGSVVLRDVPANCTVVGIPGRIVYRSGGRV-DPLE 179 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQL 206 H +PD ++R L IE + +Q+ Sbjct: 180 HNNLPDSEAQAIRALVDRIESLEQQI 205 >PA3816 [E] COG1045 Serine acetyltransferase Length = 258 Score = 235 bits (600), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 116/219 (52%), Positives = 151/219 (68%), Gaps = 13/219 (5%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 MF+ ++ DI++VF DPAAR+ EV+ Y GLHA+W HR+AH + W LAR+VS Sbjct: 1 MFERVREDIQSVFHRDPAARNALEVLTCYPGLHAVWLHRLAHGLWTSGWKWLARLVSNFG 60 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 R+MTG+EIHPGA IG R FIDHGMGIVIGET EIGDDV +YQGVTLGGT KGKRHPT+ Sbjct: 61 RWMTGIEIHPGARIGRRFFIDHGMGIVIGETAEIGDDVTLYQGVTLGGTTWNKGKRHPTL 120 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKR-NGERVSDRL 179 GNNVV+G+GAKVLG F +G+ +GSNAVV +EVP +TVVG PGR++ R + E+ + R Sbjct: 121 GNNVVVGAGAKVLGPFTVGEGAKVGSNAVVTKEVPPGATVVGIPGRIIMREDSEQQAKRQ 180 Query: 180 DHTK------------MPDPLVDSLRFLQKEIEEIREQL 206 + MPDP+ ++ L ++ + +L Sbjct: 181 AMAEKLGFDAYGVSQDMPDPVARAIGQLLDHLQAVDGRL 219 >MA2721_1 [E] COG1045 Serine acetyltransferase Length = 186 Score = 223 bits (567), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 105/184 (57%), Positives = 131/184 (71%), Gaps = 1/184 (0%) Query: 5 IKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVSRFMT 64 I+ DI+ VF+ DPAARS EV+ Y GLHAIW HR+AHS + + LAR+ S +SR +T Sbjct: 3 IREDIKTVFEKDPAARSTLEVLCCYPGLHAIWMHRVAHSLWNKDLLFLARLTSHISRALT 62 Query: 65 GVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTVGNNV 124 G+EIHPGA +G R+FIDHG G+VIGET E+GDDV+IY GV LGGT EK KRHPTV NN Sbjct: 63 GIEIHPGAKLGKRVFIDHGSGVVIGETAEVGDDVLIYMGVVLGGTALEKKKRHPTVENNA 122 Query: 125 VIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVV-KRNGERVSDRLDHTK 183 V+GSGA VLG IG +G+ +VV+R VP +TVVG P R+ S++LDH K Sbjct: 123 VLGSGAIVLGPITIGRGAKVGAGSVVVRSVPPEATVVGVPARIAGTPQPSAPSEQLDHNK 182 Query: 184 MPDP 187 +PDP Sbjct: 183 LPDP 186 >RSc1162 [E] COG1045 Serine acetyltransferase Length = 268 Score = 209 bits (533), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 102/172 (59%), Positives = 128/172 (74%), Gaps = 1/172 (0%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 MF I+ DIRA+ + DPAARS +EV+ Y GLHAI HR AH+ + + L R +S + Sbjct: 21 MFTRIREDIRAIMERDPAARSRWEVLTCYPGLHAIIVHRAAHACWGAGFRWLGRWLSHLG 80 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKG-KRHPT 119 RF+TG+EIHPGA +G R+FIDHGMG+VIGET EIGDD IYQGVTLGGT KG KRHPT Sbjct: 81 RFLTGIEIHPGAQVGRRVFIDHGMGVVIGETAEIGDDCTIYQGVTLGGTSLYKGAKRHPT 140 Query: 120 VGNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRN 171 +G VV+ +GAKVLG F IGD +GSNAVVL+ VP+ +T VG P R+++R+ Sbjct: 141 LGKGVVVSAGAKVLGGFVIGDGARVGSNAVVLKPVPAGATAVGIPARILERD 192 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,531,575 Number of extensions: 818092 Number of successful extensions: 4309 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 4243 Number of HSP's successfully gapped: 57 Length of query: 230 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 127 Effective length of database: 26,499,029 Effective search space: 3365376683 Effective search space used: 3365376683 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)