BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0077.p (1120 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0137 [R] COG2409 Predicted drug exporters of the RND superfamily 1507 0.0 STM0458 [E] COG0031 Cysteine synthase 925 0.0 MTH1050 [K] COG0085 DNA-directed RNA polymerase, beta subunit/14... 240 1e-62 MJ1041 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140... 233 2e-60 AF1887 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140... 221 5e-57 >ML0137 [R] COG2409 Predicted drug exporters of the RND superfamily Length = 1196 Score = 1507 bits (3902), Expect = 0.0, Method: Compositional matrix adjust. Identities = 729/1105 (65%), Positives = 890/1105 (80%), Gaps = 16/1105 (1%) Query: 2 LEFIDYSLGEPKYTVDDAKERDVTYAAPLRVKVRLINKETGEVKEQEVFMGDFPLMTETG 61 LEF+ Y + PKYTV++A+ D Y+AP+ V RL+NKETGE+K QEVF GDFPLMTE G Sbjct: 64 LEFVGYEMKTPKYTVEEARAHDANYSAPIYVTFRLVNKETGELKTQEVFFGDFPLMTEMG 123 Query: 62 TFIINGAERVIVSQLVRSPSVYFSTKVDKNGKKTYTATVIPNRGAWLELETDAKDIMYVR 121 TFI NG+ER+IVSQLVRSP YF K DKNG +++ T IPNRGAW EL+TDAK I YVR Sbjct: 124 TFINNGSERLIVSQLVRSPGSYFHLKADKNGLESFGHTTIPNRGAWFELDTDAKGIGYVR 183 Query: 122 IDRTRKIPVTVLLRALGFGSDAEILELLGNDEYIRNTLDKD---NTDST--EKALIEIYE 176 IDRTRK+ T +LRALGFGSD EILELLG + + +T+ KD N T E+AL +IY+ Sbjct: 184 IDRTRKLTFTTMLRALGFGSDEEILELLGETQLLTDTIAKDVHKNPADTRVEEALKDIYD 243 Query: 177 RLRPGEPPTLDNAKSLLVARFFDPKRYDLANVGRYKINKKLHIKNRLFNQRLAQPLVDES 236 RLRPGEP T D+++ LLVARFFDPKRYD A VGRYK NKKL +KNRL LA+P+VD Sbjct: 244 RLRPGEPKTADSSRGLLVARFFDPKRYDFAPVGRYKFNKKLALKNRLLGLTLAEPIVDPE 303 Query: 237 TGEILAESGQMVDRRLLDELIPYFEKDVAAKTYRVTGGVLDSEDIPLQTIDVFSPIEEGR 296 TGEIL + +V R +LD + P + + + + E I LQ+I V+SP + R Sbjct: 304 TGEILVNTDTLVTRDVLDLIEPLLDNGLGNFVVEPSDDAVIPEPITLQSIKVYSPKDPER 363 Query: 297 VIKLIANGNIDKSVKHITQADIISSISYFINLLHGIGNTDDIDHLGNRRLRSVGELLQNQ 356 V+ L++NGN D + +T AD+IS+ISY++ L GIG DDIDHLGNRR+RSVGELLQNQ Sbjct: 364 VVTLLSNGNPDAECRVLTPADVISNISYWLGLAEGIGKVDDIDHLGNRRIRSVGELLQNQ 423 Query: 357 FRIGLSRMERVVRERMSIQDANAITPQALINIRPVIASIKEFFGSSQLSQFMDQTNPLAE 416 RIGLSRMERV+RERMS + ITPQ LINIRPV ASIKEFFGSSQLSQFMDQ NPL+E Sbjct: 424 VRIGLSRMERVIRERMSSSENENITPQGLINIRPVTASIKEFFGSSQLSQFMDQHNPLSE 483 Query: 417 LTHKRRLSALGPGGLTRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLSTFARINE 476 L+HKRR SALGPGG++R+RA +EVRDVH++HYGRMCPIETPEGPNIGLIN+LS++A++NE Sbjct: 484 LSHKRRFSALGPGGISRDRASYEVRDVHYTHYGRMCPIETPEGPNIGLINNLSSYAKVNE 543 Query: 477 YGFIEAPYRWVDPKTGKVTEHIDYLTADEEDNYVVAQANVQIDEDGTFKEDMVIVRYNKD 536 YGFI +PYR VD G VT+ ++YLTADEEDNY VAQAN + ED F + V+ R+ Sbjct: 544 YGFIMSPYRRVDRVNGVVTDEVEYLTADEEDNYTVAQANSPLTEDSRFANETVMARH--- 600 Query: 537 SDNITTMPSNRVDYMDVSPKQVVSVATALIPFLENDDSNRALMGSNMQRQAVPLLIPKAP 596 + N + ++ DYMDVSPKQV++VA A IPFLENDDSNRALMG+NMQRQAVPL+ P AP Sbjct: 601 TGNNIEVEASTADYMDVSPKQVIAVAAACIPFLENDDSNRALMGANMQRQAVPLIDPHAP 660 Query: 597 LVGTGMEHKSAKDSGVCIVSKYDGIIERSSANEIWLRRVEMVEGKEVKGDIVKYKLHKFM 656 +GTGMEH++A+DSG +++K+ G++E NEI +RR G++ Y + K+ Sbjct: 661 WIGTGMEHQTARDSGAALIAKHAGVVEYVDGNEIRVRRT--------SGELDIYNITKYR 712 Query: 657 RSNQGTCINQRPLAKRGDIVKKGDILADGPSTEMGELALGRNVVVAFMTWEGYNYEDAIL 716 RSN GT NQRPLA+ G+ V+KGDI+ADGPS E GE+ALG+N +VA+MTWEGYN+EDA++ Sbjct: 713 RSNSGTSYNQRPLARLGEKVEKGDIIADGPSMENGEMALGQNPLVAYMTWEGYNFEDAVI 772 Query: 717 LSEKLVKEDVYTSIHIEEYESEARDTKLGPEEITRDIPNVGEEALRNLDERGIIRIGAEI 776 +SE+L+K+DVYTSI IEEYESE RDTKLGPEEITR+IPNVG+EAL+NLDE GIIRIGAE+ Sbjct: 773 MSERLIKDDVYTSIAIEEYESETRDTKLGPEEITREIPNVGDEALKNLDESGIIRIGAEV 832 Query: 777 NAGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDTSLRVPHGSDGIIVDVKVFTRE 836 GD+LVGKVTPKG T+ T EERLL AIFGEKAREVRDTSLRVPHG GI+ DV+VFTRE Sbjct: 833 KDGDLLVGKVTPKGETDPTPEERLLRAIFGEKAREVRDTSLRVPHGGGGIVHDVRVFTRE 892 Query: 837 NGDELPPGVNQLVRVYIAQKRKISEGDKMAGRHGNKGVVARILPEEDMPFLPDGTPVQVV 896 NGDELP GVN+LVRV+IAQKRKI GDKMAGRHGNKGVV+ I+P EDMP+LPDGTP+ ++ Sbjct: 893 NGDELPSGVNKLVRVFIAQKRKIHVGDKMAGRHGNKGVVSNIVPMEDMPYLPDGTPIDIM 952 Query: 897 LNPLGVPSRMNIGQVLEVHLGMAALRLGIHVATPVFDGAREYDVFDTMEEAGMQRNGKTV 956 LNPLGVPSRMNIGQV+E+HLGMAA LGIH+ATPVFDGA + D++DT++EAGM + KTV Sbjct: 953 LNPLGVPSRMNIGQVMELHLGMAARTLGIHIATPVFDGASDEDIWDTVKEAGMADDAKTV 1012 Query: 957 LYDGRTGERFEREVTVGVMHMIKLAHMVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFG 1016 LYDGRTGE F+ ++VGVM+MIKL HMVDDK+HARS GPYSLVTQQPLGGKAQFGGQRFG Sbjct: 1013 LYDGRTGEPFDNRISVGVMYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFG 1072 Query: 1017 EMEVWALEAYGAAYTLQEILTVKSDDVVGRVKTYESIVKGENLPEPGVPEAFKVLIKELQ 1076 EMEVWALEAYGAA LQEILT KSDDV+GR + YE+IVKGE +P+PG+PE+F+VL+KELQ Sbjct: 1073 EMEVWALEAYGAANVLQEILTYKSDDVIGRTRAYEAIVKGERIPKPGLPESFRVLVKELQ 1132 Query: 1077 SLGMDVKILSGDEQEIEMKELDDED 1101 SLG+D+K+L D ++++ELD+++ Sbjct: 1133 SLGLDMKVLDADRNVLDLRELDEDE 1157 >STM0458 [E] COG0031 Cysteine synthase Length = 351 Score = 925 bits (2391), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/962 (0%), Positives = 0/962 (0%), Gaps = 53/962 (5%) Query: 170 ALIEIYERLRPGEPPTLDNAKSLLVARFFDPKRYDLANVGRYKINKKLH-------IKNR 222 Sbjct: 315 374 Query: 223 LFNQRLAQPLVDESTGEILAESGQMVDRRLLDELIPYFEK----DVAAKTYRVTGGVLDS 278 Sbjct: 375 --- 431 Query: 279 EDIPLQTIDVFSPIEEGRVIKLIANGNIDKSVKHITQADIISSISYFINLLHGIGNT--D 336 Sbjct: 432 ------ 485 Query: 337 DIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMS-IQDANAITPQALINIRPVIASI 395 Sbjct: 486 545 Query: 396 KEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLTRERAGFEVRDVHHSHYGRMCPIE 455 Sbjct: 546 605 Query: 456 TPEGPNIGLINSLSTFARINEYGFIEAPYRWVDPKTGKVTEHIDYLTADEEDNYVVAQAN 515 Sbjct: 606 -- 663 Query: 516 VQIDEDGTFKEDMVIVRYNKDSDNITTMPSNRVDYMDVSPKQVVSVATALIPFLENDDSN 575 Sbjct: 664 --- 720 Query: 576 RALMGSNMQRQAVPLLIPKAPLVGTGMEHKSAKDSGVCIVSKYDGIIERSSANEIWLRRV 635 Sbjct: 721 780 Query: 636 EMVEGKEV----KGDIV--KYKLHKFMRSNQGTCINQRPLAKRGDIVKKGDILADGPSTE 689 Sbjct: 781 -- 838 Query: 690 MGELALGRNVVVAFMTWEGYNYEDAILLSEKLVKEDVYTSIHIEEYESEARDTKLGPEEI 749 Sbjct: 839 898 Query: 750 TRDIPNVGEEALRNLDERGIIRIGAEI------NAGDILVGKVTPKGVTELTAEERLLHA 803 Sbjct: 899 958 Query: 804 IFGEKAREVRDTSLRVPHGSDGIIVDVKVFTRENGDELPPGVNQLVRVYIAQKRKISEGD 863 Sbjct: 959 1018 Query: 864 KMAGRHGNKGVVARILPEEDMPFLPDGTPVQVVLNPLGVPSRMNIGQVLEVHLGMAALRL 923 Sbjct: 1019 1078 Query: 924 GIHVATPVFDGAREYDVFDTMEEAGMQR-----------NGKTVLYDGRTGERFEREVTV 972 Sbjct: 1079 1138 Query: 973 GVMHMIKLAHMVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL 1032 Sbjct: 1139 1198 Query: 1033 QEILTVKSDDVVGRVKTYESIVKGENLPEPGVPEAFKVLIKELQSLGMDVKILSGDEQEI 1092 Sbjct: 1199 1258 Query: 1093 EM 1094 Sbjct: 1259 1260 Score = 121 bits (303), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 5/167 (2%), Positives = 28/167 (16%), Gaps = 12/167 (7%) Query: 2 LEFIDYSLGEPKYTVDDAKERDVTYAAPLRVKVRLINKETGEVKEQEVFMGDFPLMTETG 61 ++ + + ++ + V ++++ + + G + Sbjct: 70 IKEGTPIIEASSGSTAISEAWFARLLGLPFIAVMPACTAKRKIEQIQFYGGHCHFVESAC 129 Query: 62 TFIINGAERVIVSQLVRSPSVYFSTK-VDKNGKKTYTATVIPNRGAWLELETDAKD-IMY 119 ++ + D + I+ Sbjct: 130 EIYAASERLAHELNGHYMDQFTYAERATD---WRGNNNIADSIFRQMRNELHPVPRFIVM 186 Query: 120 VRIDRTRKIPVTVLLRALGFGSDAEILELLGNDEYIRNTLDKDNTDS 166 ++ N++ Sbjct: 187 SAGT-GGTSATIGRYIRCQGYDTQLMVVDPE------NSVFLPYWQD 226 >MTH1050 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140 kD subunit Length = 268 Score = 240 bits (612), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 0/417 (0%), Positives = 0/417 (0%), Gaps = 40/417 (9%) Query: 696 GRNVVVAFMTWEGYNYEDAILLSEKLVKEDVYTSIHIEEYESEARDTKLGPEEITRDIPN 755 + + L E V T + L ++ Sbjct: 203 VKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTETVRTEEAVLATLS-VFNMLT 261 Query: 756 VG------EEALRNLDERGIIRIGAEINAGDILVGKVTPKGVTELTAEERLLHAI--FGE 807 Sbjct: 262 QI--------- 312 Query: 808 KAREVRDTSLRVPHGSDGIIVDVKVFTRENGDELPPGVNQLVRVYIAQKRKISEGDKMAG 867 Sbjct: 313 ------- 365 Query: 868 RHGNKGVVARILPEEDMPFLPDGTPVQVVLNPLGVPSRMNIGQVLEVHLGMAALRLGIHV 927 Sbjct: 366 425 Query: 928 ATPVFDGAREYDVFDTMEEAGMQRNGKTVLYDGRTGERFEREVTVGVMHMIKLAHMVDDK 987 Sbjct: 426 485 Query: 988 IHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDV---- 1043 Sbjct: 486 545 Query: 1044 ---VGRVKTYESIVKGENLP--------EPGVPEAFKVLIKELQSLGMDVKILSGDE 1089 Sbjct: 546 602 >MJ1041 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140 kD subunit Length = 636 Score = 233 bits (593), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 149/427 (34%), Positives = 231/427 (54%), Gaps = 45/427 (10%) Query: 696 GRNVVVAFMTWEGYNYEDAILLSEKLVKEDVYTSIHIEEYESEARDTKLGPEEITR-DIP 754 G+N VVA M++EGYN EDAI+ ++ + + S Y++ R G ++ R +IP Sbjct: 208 GQNFVVAIMSYEGYNMEDAIVFNKSAIDRGLGRSTFFRTYDACERRYPGG--QMDRFEIP 265 Query: 755 NVG------EEALRNLDERGIIRIGAEINAGDILVGKVTPKGVTELTAEERLLHAIFGEK 808 + G EE R L+E GI+ + + + GD++VGK +P E H I + Sbjct: 266 DKGVRGYRSEECYRYLEEDGIVAVESHVKGGDVIVGKTSPPRFLEE-------HEITIQV 318 Query: 809 AREVRDTSLRVPHGSDGIIVDVKVFTRENGDELPPGVNQLVRVYIAQKRKISEGDKMAGR 868 + RD+S+ V HG +G I V + + G N+LV+V + R GDK A R Sbjct: 319 KPQRRDSSVVVRHGEEGYIDKVILTETKEG-------NRLVKVKVRDLRIPELGDKFASR 371 Query: 869 HGNKGVVARILPEEDMPFLPDGTPVQVVLNPLGVPSRMNIGQVLEVHLGMAALRLGIHVA 928 HG KGV+ +P+ED+PF G +++NP +PSRM +GQ+LE+ G G + Sbjct: 372 HGQKGVMGLTVPQEDLPFTESGIVPDIIINPHAIPSRMTVGQLLEMLGGKVGALEGRRID 431 Query: 929 TPVFDGAREYDVFDTMEEAGMQRNGKTVLYDGRTGERFEREVTVGVMHMIKLAHMVDDKI 988 +F G +E+D+ +E G + +GK V+YDG+TG++FE E+ +G+ + KL H+V KI Sbjct: 432 GTIFSGEKEWDLRKALEALGFKHHGKEVMYDGKTGKKFEVEIYIGIAYYQKLHHLVAGKI 491 Query: 989 HARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSD--DVVGR 1046 HARS GP ++T+QP G+A+ GG RFGEME L +GAA L+E L +SD D+ Sbjct: 492 HARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLMDESDPYDICIC 551 Query: 1047 VKT--------------------YESIVKGENLPEPGVPEAFKVLIKELQSLGMDVKILS 1086 K E++ + +P +P AFK+L+ EL+S+ + +I Sbjct: 552 SKCGDFAILDYKRGLKYCPICGEIENLYSSKKIPFVRIPYAFKLLLDELKSMCILPRIKV 611 Query: 1087 GDEQEIE 1093 D+ E+E Sbjct: 612 RDKVELE 618 >AF1887 [K] COG0085 DNA-directed RNA polymerase, beta subunit/140 kD subunit Length = 604 Score = 221 bits (563), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 151/420 (35%), Positives = 223/420 (53%), Gaps = 37/420 (8%) Query: 692 ELALGRNVVVAFMTWEGYNYEDAILLSEKLVKEDVYTSIHIEEYESEARDTKLGPEEITR 751 E G+N VVA +++EGYN EDA+++++ V+ + S YESE G E+ Sbjct: 199 ERPAGQNFVVAVLSYEGYNIEDALIMNKGSVERGLGRSFFYRTYESEEMRYPGGQED-KF 257 Query: 752 DIPNV------GEEALRNLDERGIIRIGAEINAGDILVGKVTPKGVTELTAEERLLHAIF 805 +IP G EA +LDE G+I E+ D+L+G+ +P E E Sbjct: 258 EIPGADISGFRGAEAYAHLDEDGLIFPETEVGPDDVLIGRTSPPRFLEEPTE-------L 310 Query: 806 GEKAREVRDTSLRVPHGSDGIIVDVKVFTRENGDELPPGVNQLVRVYIAQKRKISEGDKM 865 G ++ R+TS+ + G++ V + E G ++L +V + R GDK Sbjct: 311 GISPQKRRETSVTMRSNEKGVVDAVFLMQSEGG-------SKLAKVRVRDLRIPELGDKF 363 Query: 866 AGRHGNKGVVARILPEEDMPFLPDGTPVQVVLNPLGVPSRMNIGQVLEVHLGMAALRLGI 925 A RHG KGVV ++PEEDMPF G +++NP +PSRM +G VLE+ G G Sbjct: 364 ASRHGQKGVVGLLVPEEDMPFTESGIVPDLIINPHAIPSRMTVGHVLEMIGGKVGSLEGR 423 Query: 926 HVATPVFDGAREYDVFDTMEEAGMQRNGKTVLYDGRTGERFEREVTVGVMHMIKLAHMVD 985 V +F G E D+ T+++ G GK V+YDG TG R+ ++ VGV++ KL HMV Sbjct: 424 RVDGSLFHGESEEDLRATLKKYGFSHTGKEVMYDGITGRRYLVDIFVGVIYYQKLYHMVS 483 Query: 986 DKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDV-- 1043 KIHARS GP ++T+QP G+A+ GG RFGEME L +GAA L++ L +SD V Sbjct: 484 SKIHARSRGPVQVLTRQPTEGRARKGGLRFGEMERDVLIGHGAALLLKDRLLEESDKVEV 543 Query: 1044 -----VGRVKTYE--------SIVKGE-NLPEPGVPEAFKVLIKELQSLGMDVKILSGDE 1089 G V TY+ I E N+ + + AFK+L+ EL+S+ + +I+ GD+ Sbjct: 544 WVCGNCGHVATYDYRRNTAYCHICDDESNIHKVEMSYAFKLLLDELKSMMIAPRIILGDK 603 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 86,893,783 Number of extensions: 3916197 Number of successful extensions: 8880 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 8806 Number of HSP's successfully gapped: 35 Length of query: 1120 Length of database: 38,378,431 Length adjustment: 117 Effective length of query: 1003 Effective length of database: 24,884,353 Effective search space: 24959006059 Effective search space used: 24959006059 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 121 (51.2 bits)