BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0069.p (305 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value HI0632 [J] COG0050 GTPases - translation elongation factors 402 e-112 HI0578 [J] COG0050 GTPases - translation elongation factors 402 e-112 NMB0124 [J] COG0050 GTPases - translation elongation factors 401 e-112 NMA0149 [J] COG0050 GTPases - translation elongation factors 401 e-112 NMA0134 [J] COG0050 GTPases - translation elongation factors 401 e-112 >HI0632 [J] COG0050 GTPases - translation elongation factors Length = 394 Score = 402 bits (1034), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/305 (64%), Positives = 234/305 (76%), Gaps = 2/305 (0%) Query: 1 MITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCXXXXXXXXXXXXX 60 MITGAAQMDGAILVV+A DGPMPQTREHILL RQVGVPYI+VFLNKC Sbjct: 92 MITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE 151 Query: 61 XXXXXXXNEYEFPGDDTPIIRGSAREALQNPDGDYAQKIVEMFETIDTYIPLPERDTDKP 120 ++Y+FPGDDTPI+RGSA +AL N ++ +KI+E+ +DTYIP PER D+P Sbjct: 152 MEVRELLSQYDFPGDDTPIVRGSALQAL-NGVAEWEEKILELANHLDTYIPEPERAIDQP 210 Query: 121 FLMPVEDVFSITGRGTVATGRVERGTVKXXXXXXXXXXHEDKKKSVVTGVEMFRKLLDSA 180 FL+P+EDVFSI+GRGTV TGRVERG ++ +D K+ VTGVEMFRKLLD Sbjct: 211 FLLPIEDVFSISGRGTVVTGRVERGIIRTGDEVEIVGI-KDTAKTTVTGVEMFRKLLDEG 269 Query: 181 QAGDNIGALLRGVDRNMIERGQVLAKPNSVNPHTEFTAQIYVLTKEEGGRHKPFFTGYRP 240 +AG+NIGALLRG R IERGQVLAKP S+ PHT+F +++YVL+K+EGGRH PFF GYRP Sbjct: 270 RAGENIGALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKDEGGRHTPFFKGYRP 329 Query: 241 QFYFRTTDVTGIINLPEGTEMVMPGDNITVTVQLISPIAIEEGTKFSIREGGRTVGAGTV 300 QFYFRTTDVTG I LPEG EMVMPGDNI +TV LI PIA+++G +F+IREGGRTVGAG V Sbjct: 330 QFYFRTTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDQGLRFAIREGGRTVGAGVV 389 Query: 301 ASIQK 305 A I K Sbjct: 390 AKIIK 394 >HI0578 [J] COG0050 GTPases - translation elongation factors Length = 394 Score = 402 bits (1034), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/305 (64%), Positives = 234/305 (76%), Gaps = 2/305 (0%) Query: 1 MITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCXXXXXXXXXXXXX 60 MITGAAQMDGAILVV+A DGPMPQTREHILL RQVGVPYI+VFLNKC Sbjct: 92 MITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE 151 Query: 61 XXXXXXXNEYEFPGDDTPIIRGSAREALQNPDGDYAQKIVEMFETIDTYIPLPERDTDKP 120 ++Y+FPGDDTPI+RGSA +AL N ++ +KI+E+ +DTYIP PER D+P Sbjct: 152 MEVRELLSQYDFPGDDTPIVRGSALQAL-NGVAEWEEKILELANHLDTYIPEPERAIDQP 210 Query: 121 FLMPVEDVFSITGRGTVATGRVERGTVKXXXXXXXXXXHEDKKKSVVTGVEMFRKLLDSA 180 FL+P+EDVFSI+GRGTV TGRVERG ++ +D K+ VTGVEMFRKLLD Sbjct: 211 FLLPIEDVFSISGRGTVVTGRVERGIIRTGDEVEIVGI-KDTAKTTVTGVEMFRKLLDEG 269 Query: 181 QAGDNIGALLRGVDRNMIERGQVLAKPNSVNPHTEFTAQIYVLTKEEGGRHKPFFTGYRP 240 +AG+NIGALLRG R IERGQVLAKP S+ PHT+F +++YVL+K+EGGRH PFF GYRP Sbjct: 270 RAGENIGALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKDEGGRHTPFFKGYRP 329 Query: 241 QFYFRTTDVTGIINLPEGTEMVMPGDNITVTVQLISPIAIEEGTKFSIREGGRTVGAGTV 300 QFYFRTTDVTG I LPEG EMVMPGDNI +TV LI PIA+++G +F+IREGGRTVGAG V Sbjct: 330 QFYFRTTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDQGLRFAIREGGRTVGAGVV 389 Query: 301 ASIQK 305 A I K Sbjct: 390 AKIIK 394 >NMB0124 [J] COG0050 GTPases - translation elongation factors Length = 394 Score = 401 bits (1030), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/303 (63%), Positives = 230/303 (75%), Gaps = 2/303 (0%) Query: 1 MITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCXXXXXXXXXXXXX 60 MITGAAQMDGAILV SAADGPMPQTREHILL+RQVGVPYI+VF+NKC Sbjct: 92 MITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVE 151 Query: 61 XXXXXXXNEYEFPGDDTPIIRGSAREALQNPDGDYAQKIVEMFETIDTYIPLPERDTDKP 120 + Y+FPGDD PI++GSA +AL+ D Y +KI E+ +D+YIP PER DKP Sbjct: 152 MEIRDLLSSYDFPGDDCPIVQGSALKALEG-DAAYEEKIFELAAALDSYIPTPERAVDKP 210 Query: 121 FLMPVEDVFSITGRGTVATGRVERGTVKXXXXXXXXXXHEDKKKSVVTGVEMFRKLLDSA 180 FL+P+EDVFSI+GRGTV TGRVERG + E +K+ TGVEMFRKLLD Sbjct: 211 FLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKE-TQKTTCTGVEMFRKLLDEG 269 Query: 181 QAGDNIGALLRGVDRNMIERGQVLAKPNSVNPHTEFTAQIYVLTKEEGGRHKPFFTGYRP 240 QAGDN+G LLRG R +ERGQVLAKP ++ PHT+F A++YVL+KEEGGRH PFF YRP Sbjct: 270 QAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRP 329 Query: 241 QFYFRTTDVTGIINLPEGTEMVMPGDNITVTVQLISPIAIEEGTKFSIREGGRTVGAGTV 300 QFYFRTTDVTG + L EG EMVMPG+N+T+TV+LI+PIA+EEG +F+IREGGRTVGAG V Sbjct: 330 QFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVV 389 Query: 301 ASI 303 +S+ Sbjct: 390 SSV 392 >NMA0149 [J] COG0050 GTPases - translation elongation factors Length = 434 Score = 401 bits (1030), Expect = e-112, Method: Compositional matrix adjust. Identities = 15/303 (4%), Positives = 48/303 (15%), Gaps = 2/303 (0%) Query: 1 MITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCXXXXXXXXXXXXX 60 + ++ + + + I K Sbjct: 92 KTLLFDTFNDFFQLLRYHNVSYDGISDLMDFIYPRSRSTIFRAFYKEMEQETIPYSENIH 151 Query: 61 XXXXXXXNEYEFPGDDTPIIRGSAREALQNPDGDYAQKIVEMFETIDTYIPLPERDTDKP 120 + E + A+ + + L + Sbjct: 152 MVHYDEQHPKEGRCQKYRLTLLDAKAQRTI-ADELFKDKSPETIKKFLKKNLDASEPVFI 210 Query: 121 FLMPVEDVFSITGRGTVATGRVERGTVKXXXXXXXXXXHEDKKKSVVTGVEMFRKLLDSA 180 + I + + + + Sbjct: 211 VTDFDKRYPDILKEIFRDKLVHQYCLMHLNKLIVNDFPKN-TTIEQELLKYRLLSIFYNR 269 Query: 181 QAGDNIGALLRGVDRNMIERGQVLAKPNSVNPHTEFTAQIYVLTKEEGGRHKPFFTGYRP 240 + L+ + N+I + + + + + + Sbjct: 270 ENEIKFLQKLQSEELNVINNKEKHQEWIKKAKKEFCQYRHKLKLEIRRKKENLPRNSLEK 329 Query: 241 QFYFRTTDVTGIINLPEGTEMVMPGDNITVTVQLISPIAIEEGTKFSIREGGRTVGAGTV 300 Y + I E + + N + + E + T Sbjct: 330 AKYNFDKLMENIRTYDEKVQKRLWMINKHWLNLTLFHYLPGAPATNNPIESYYSKSLKTD 389 Query: 301 ASI 303 Sbjct: 390 NKK 392 >NMA0134 [J] COG0050 GTPases - translation elongation factors Length = 132 Score = 401 bits (1030), Expect = e-112, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 2/303 (0%) Query: 1 MITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCXXXXXXXXXXXXX 60 Sbjct: 92 151 Query: 61 XXXXXXXNEYEFPGDDTPIIRGSAREALQNPDGDYAQKIVEMFETIDTYIPLPERDTDKP 120 Sbjct: 152 - 210 Query: 121 FLMPVEDVFSITGRGTVATGRVERGTVKXXXXXXXXXXHEDKKKSVVTGVEMFRKLLDSA 180 Sbjct: 211 - 269 Query: 181 QAGDNIGALLRGVDRNMIERGQVLAKPNSVNPHTEFTAQIYVLTKEEGGRHKPFFTGYRP 240 Sbjct: 270 329 Query: 241 QFYFRTTDVTGIINLPEGTEMVMPGDNITVTVQLISPIAIEEGTKFSIREGGRTVGAGTV 300 Sbjct: 330 389 Query: 301 ASI 303 Sbjct: 390 392 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,737,274 Number of extensions: 801947 Number of successful extensions: 2806 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 2669 Number of HSP's successfully gapped: 50 Length of query: 305 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 199 Effective length of database: 26,153,027 Effective search space: 5204452373 Effective search space used: 5204452373 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)