BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0061.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1497 [C] COG0437 Fe-S-cluster-containing hydrogenase componen... 318 5e-87 YEL050c [J] COG0090 Ribosomal protein L2 208 6e-54 SPCC16C4.15 [J] COG0090 Ribosomal protein L2 194 2e-49 MTH514 [H] COG1429 Cobalamin biosynthesis protein CobN and relat... 139 3e-33 MTH550_2 [ER] COG1387 Histidinol phosphatase and related hydrola... 139 3e-33 >STM1497 [C] COG0437 Fe-S-cluster-containing hydrogenase components 1 Length = 205 Score = 318 bits (815), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 0/262 (0%), Positives = 0/262 (0%), Gaps = 3/262 (1%) Query: 1 MSVSTFEEITTNQPEKSLLAPLSKKAGRNNQGKITVRHHGGGHKRKYRIIDFKR-TKDGI 59 + + ++ + Y I Sbjct: 16 GCKTCELACKDYKDLTPDVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSISCNHCEDPAC 75 Query: 60 PGSVATIEYDPNRTSNIALIHYADGEKRYIIAPKGLKVGDKVESGPAADIKIGNSLPLEN 119 + + + RY + G + + Sbjct: 76 TKVCPSGAMHKRDDGFVVVNEEVCIGCRYCHMACPYGAPQYNAAKGHMTKCDGCYDRVAD 135 Query: 120 IPVGTVIHNIELKPGKGGQLVRAAGTEAQLLGKEEKYVSVRLNSGEVRRILSVCRATIGS 179 + + L+ G + +L + V + + R T + Sbjct: 136 GKKPICVESCPLRALDFGPIDELRKKHGELAAVAPLPRAHFTKPNIVIKPNANSRPTGDT 195 Query: 180 VGNEDHELIKIGKAGRSRWLGQRPEVRGVVMNPNDHPHGGGEGRAPIGRKSPMSPWGKPT 239 Sbjct: 196 -- 253 Query: 240 LGYKTRKKNKASDKYIVRRRTK 261 Sbjct: 254 275 >YEL050c [J] COG0090 Ribosomal protein L2 Length = 311 Score = 208 bits (530), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 0/265 (0%), Positives = 0/265 (0%), Gaps = 26/265 (9%) Query: 6 FEEITTNQPEKSLLAPLSKKAGRNNQGKITVRHHGGGHKRKYRIIDFKRTKDGIPGSVAT 65 E + K N + K + + Sbjct: 111 KEAQLKELTKALGNNWNLFKGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEK-ATYG 169 Query: 66 IEYDPNRTSNIALI-HYADGEKRYIIAPKGLKVGDKVES-------------GPAADIKI 111 I + +I + + + A Sbjct: 170 GANTAKLFQIAQGIRTWGTAAAILLIFVAVFLISNTIRITILSRQREIQIMRLVGAKNGY 229 Query: 112 --------GNSLPLENIPVGTVIHNIELKPGKGGQLVRAAGTEAQLLGK--EEKYVSVRL 161 G + L + +I + + + + + Sbjct: 230 IRWPFFLEGAWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKLAMFAPKEFIPAIVGLM 289 Query: 162 NSGEVRRILSVCRATIGSVGNEDHELIKIGKAGRSRWLGQRPEVRGVVMNPNDHPHGGGE 221 Sbjct: 290 349 Query: 222 GRAPIGRKSPMSPWGKPTLGYKTRK 246 Sbjct: 350 - 373 >SPCC16C4.15 [J] COG0090 Ribosomal protein L2 Length = 311 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 0/281 (0%), Positives = 0/281 (0%), Gaps = 35/281 (12%) Query: 13 QPEKSLLAPLSKKAGRNNQGKITVRHHGGGHKRKYRIIDFKRTKDGIPGSVATIEYDPNR 72 L L+ N + + KD ++ ++ Sbjct: 36 LVGLFLSVILNTSKISNGIENNVRIVAYMKLDVHDQETKIQDPKDNK-KTINNPDHLKIY 94 Query: 73 TSNIALIHYADGE---KRYIIAPKGLKVGDKVESG------------------------- 104 S + + E K + +G+ Sbjct: 95 DSIKKVPNVKTVEFSSKEAQLKELTKALGNNWNLFKGDANPLYDAYIVEADKPANVKSVT 154 Query: 105 ---PAADIKIGNSLPLENIPVGTVIHNIELKPGKGGQLVRAAGTEAQLLGKEEKYVSVRL 161 + N I G ++ + + R Sbjct: 155 SKISKLSGIEKATYGGANTAKLFQIAQGIRTWGTAAAILLIFVAVFLISNTIRITILSRQ 214 Query: 162 NSGEVRRILSVCRATIGSVGNEDHELIKIGKAGRSRWLGQRPEVRGVVMNPNDHPHGGGE 221 ++ R++ I + + + + + V Sbjct: 215 REIQIMRLVGAKNGYIRWPFFLEGAWVGLLGSIIPGLIVAWAYRLAFVSLTPSLQSQKLA 274 Query: 222 GRAPIGRKSPMSPWGKPTL-GYKTRKKNKASDKYIVRRRTK 261 A P + Sbjct: 275 MFA-PKEFIPAIVGLMAVIGILIGS- 313 >MTH514 [H] COG1429 Cobalamin biosynthesis protein CobN and related Mg-chelatases Length = 211 Score = 139 bits (351), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 0/222 (0%), Positives = 0/222 (0%), Gaps = 7/222 (3%) Query: 22 LSKKAGRNNQGKITVRHHGGGHKRKYRIIDFKRTKDGIPGSVATIEYDPNRTSNIALIHY 81 + R + + ++ Sbjct: 6 RLEILTRLRENNPHPTTELNF-SSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAM 64 Query: 82 ADGEKRYIIAPKGLKVGDKVESGPAADIKIGNSLPLENIPVGTVIHNIELKPGKGGQLVR 141 + + ++ L Sbjct: 65 LELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANV 124 Query: 142 AAGTEAQLLGKEEKYVSVRLNSGEVRRILSVCRATIGSV---GNEDHELIKIGKAGRS-R 197 T R+ + + + ++ R Sbjct: 125 VLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGR 184 Query: 198 WLGQRP-EVRGVVMNPNDHPHGGGEGRAPIGRKSPMSPWGKP 238 + Sbjct: 185 YTCIARK- 225 >MTH550_2 [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 139 bits (351), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 9/222 (4%), Positives = 40/222 (18%), Gaps = 7/222 (3%) Query: 22 LSKKAGRNNQGKITVRHHGGGHKRKYRIIDFKRTKDGIPGSVATIEYDPNRTSNIALIHY 81 + + + + G + ++ + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQ-NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 82 ADGEKRYIIAPKGLKVGDKVESGPAADIKIGNSLPLENIPVGTVIHNIELKPGKGGQLVR 141 + +A G + + S + + + + + L Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 142 AAGTEAQLLGKEEKYVSVRLNSGEVRRILSVCRATIGSV---GNEDHELIKIGKAGRS-R 197 + +L +V ++ IG + + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 198 WLGQRP-EVRGVVMNPNDHPHGGGEGRAPIGRKSPMSPWGKP 238 + + + + ++ + Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNR-SFEVGLAEVSQY 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,042,303 Number of extensions: 927971 Number of successful extensions: 1410 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 1353 Number of HSP's successfully gapped: 45 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 115 (48.9 bits)