BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0059.p (110 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ML0418 [C] COG0778 Nitroreductase 145 2e-35 STM1495 [R] COG3381 Uncharacterized component of anaerobic dehyd... 119 1e-27 YKL180w [J] COG0091 Ribosomal protein L22 54 8e-08 YJL177w [J] COG0091 Ribosomal protein L22 53 8e-08 MTH753 [O] COG1331 Highly conserved protein containing a thiored... 48 4e-06 >ML0418 [C] COG0778 Nitroreductase Length = 346 Score = 145 bits (365), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 4/108 (3%), Positives = 12/108 (11%) Query: 3 AKAHARSVRISARKAKLVVDLIRGKQVGEAIAILRHTPKSASPVVEKLLNSAIANAEHNY 62 + + + + + Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 63 SMDVNSLFVSEVFVNQGPTMKRFRPRAMGRASRINKRTSHITLVVSEK 110 + N L K P + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREAL 114 >STM1495 [R] COG3381 Uncharacterized component of anaerobic dehydrogenases Length = 289 Score = 119 bits (298), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 4/110 (3%), Positives = 21/110 (19%) Query: 1 MEAKAHARSVRISARKAKLVVDLIRGKQVGEAIAILRHTPKSASPVVEKLLNSAIANAEH 60 +A ++ + + + + + + + L + Sbjct: 29 AKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTF 88 Query: 61 NYSMDVNSLFVSEVFVNQGPTMKRFRPRAMGRASRINKRTSHITLVVSEK 110 + ++ + +TL + Sbjct: 89 TRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPL 138 >YKL180w [J] COG0091 Ribosomal protein L22 Length = 311 Score = 53.5 bits (127), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 2/86 (2%), Positives = 9/86 (10%), Gaps = 4/86 (4%) Query: 25 RGKQVGEAIAILRHTPKSASPVVEKLLNSAIANAEHNYSMDVNSLFVSEVFVNQGPTMKR 84 K + ++ + + + Sbjct: 72 ETKIQDPKDN---KKTINNPDHLKIYDSIKKVPNVKT-VEFSSKEAQLKELTKALGNNWN 127 Query: 85 FRPRAMGRASRINKRTSHITLVVSEK 110 + V Sbjct: 128 LFKGDANPLYDAYIVEADKPANVKSV 153 >YJL177w [J] COG0091 Ribosomal protein L22 Length = 311 Score = 53.1 bits (126), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 2/86 (2%), Positives = 9/86 (10%), Gaps = 4/86 (4%) Query: 25 RGKQVGEAIAILRHTPKSASPVVEKLLNSAIANAEHNYSMDVNSLFVSEVFVNQGPTMKR 84 K + ++ + + + Sbjct: 72 ETKIQDPKDN---KKTINNPDHLKIYDSIKKVPNVKT-VEFSSKEAQLKELTKALGNNWN 127 Query: 85 FRPRAMGRASRINKRTSHITLVVSEK 110 + V Sbjct: 128 LFKGDANPLYDAYIVEADKPANVKSV 153 >MTH753 [O] COG1331 Highly conserved protein containing a thioredoxin domain Length = 153 Score = 47.8 bits (112), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 24/70 (34%), Positives = 42/70 (60%), Gaps = 1/70 (1%) Query: 40 PKSASPVVEKLLNSAIANAEHNYSMDVNSLFVSEVFVNQGPTMKRFRPRAMGRASRINKR 99 P A+ + K+L +A ANAE+ +D L + + ++GP ++ + PRA GRA+ N Sbjct: 84 PVKAAGQILKVLENAEANAEYK-GLDTEKLRIIHISSHRGPVIRGWIPRAFGRATPFNTP 142 Query: 100 TSHITLVVSE 109 T+H+ +V+ E Sbjct: 143 TTHVQIVLGE 152 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.128 0.341 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 6,669,944 Number of extensions: 226505 Number of successful extensions: 443 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 443 Number of HSP's successfully gapped: 34 Length of query: 110 Length of database: 38,378,431 Length adjustment: 78 Effective length of query: 32 Effective length of database: 29,382,379 Effective search space: 940236128 Effective search space used: 940236128 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 109 (46.6 bits)