BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0050.p (180 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC12G12.08 [J] COG0097 Ribosomal protein L6P/L9E 119 3e-27 YHR147c [J] COG0097 Ribosomal protein L6P/L9E 104 6e-23 MTH1913 [S] COG1432 Uncharacterized conserved protein 75 5e-14 MTH195 [GEPR] COG0477 Permeases of the major facilitator superfa... 75 5e-14 MTH194_2 [E] COG0069 Glutamate synthase domain 2 75 5e-14 >SPAC12G12.08 [J] COG0097 Ribosomal protein L6P/L9E Length = 213 Score = 119 bits (297), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 60/172 (34%), Positives = 101/172 (58%), Gaps = 5/172 (2%) Query: 2 SRIGRKPIAVPSGVDVTLNNAVITVKGPKGTLTRELHKDMKVTVENNEITV-----VRPS 56 S +G+K I VP + L + + GP GTL + ++ N+ + V + Sbjct: 32 SYVGKKEIIVPKNIQFDLEGEQLQITGPHGTLNMRFPNYLNLSKTNDILKVGMDANIEKQ 91 Query: 57 DNKLHRSLHGTTRSVVNNMVSGVTEGFSKSLELVGVGYRASKSGDKIVLNVGYSHPVEIT 116 K R++ GTTR+++ N V GVT + ++LVG+GYR S + I L +GY++ + ++ Sbjct: 92 ATKKQRAMWGTTRAILANNVKGVTMYWQSIIKLVGIGYRTSLNDGNIHLKIGYANDILVS 151 Query: 117 PEAGIEFEVPANTKIIVKGIDKERVGAYAAKIRSVREPEPYKGKGIKYEGER 168 ++ E P T ++++GID+++V +AAKIRS ++PEPYKGKGI +GE+ Sbjct: 152 IPTDVQVENPTPTSLVLRGIDRQKVTQFAAKIRSFKKPEPYKGKGIYVDGEK 203 >YHR147c [J] COG0097 Ribosomal protein L6P/L9E Length = 214 Score = 104 bits (260), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 76/204 (37%), Positives = 104/204 (50%), Gaps = 38/204 (18%) Query: 2 SRIGRKPIAVPSGVDVTLN-----------------NAVITVKGPKGTLTRE----LHKD 40 S++G P+ + V V++N + ITVKGPKG L+ E LH D Sbjct: 17 SQVGSLPLYISPEVQVSINALSMPRIIRKGRTSMNISQNITVKGPKGELSVEVPDFLHLD 76 Query: 41 -------MKVTVENNEITVVRPSDNKLHRSLHGTTRSVVNNMVSGVTEGFSKSLELVGVG 93 + VTV+N S++K RS+ GT RS++NN + GVTEG L VG G Sbjct: 77 KDEKHGKINVTVQN--------SEDKHQRSMWGTVRSLINNHIIGVTEGHLAVLRFVGTG 128 Query: 94 YRASKSGDKIVLNV--GYSHPVEITPEAGIEFEVPANTKIIVKGIDKERVGAYAAKIRSV 151 YRA D +NV G S + GI + PA T +I++G +K++V +AAK+R Sbjct: 129 YRAQLENDGKFVNVKVGASIKQGLDVPEGIVVKTPAPTSLIIEGCNKQQVLLFAAKLRKF 188 Query: 152 REPEPYKGKGIKYEGERIIRKEGK 175 PEPYKGKGI E I K+ K Sbjct: 189 HPPEPYKGKGIYVNDETIKLKDKK 212 >MTH1913 [S] COG1432 Uncharacterized conserved protein Length = 268 Score = 75.1 bits (183), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 7/168 (4%), Positives = 23/168 (13%), Gaps = 12/168 (7%) Query: 1 MSRIGRKPIAVPSGVDVTLNNAVITVKGPKGTLTREL-HKDMKVTVENNEITVVRPSDNK 59 + + L K + + ++ + D Sbjct: 3 RVDLSIFIPDSLTAETGDLKIKT-YKVVLIARAASIFGVKRIVIYHDDADGEARFIRDIL 61 Query: 60 LHRSLHGTTRSVVNNMVSGVTEGFSKSLELVGVGYRAS--KSGDKIVLN--VGYSHPVEI 115 + R V ++ + + L + Sbjct: 62 TYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVD 121 Query: 116 TPEAGIEFEVPANTKIIVKGIDKERVGAYAAKIRSV-----REPEPYK 158 + + + I Sbjct: 122 IGADKLALCREKL-TVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVL 168 >MTH195 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 268 Score = 75.1 bits (183), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 7/168 (4%), Positives = 23/168 (13%), Gaps = 12/168 (7%) Query: 1 MSRIGRKPIAVPSGVDVTLNNAVITVKGPKGTLTREL-HKDMKVTVENNEITVVRPSDNK 59 + + L K + + ++ + D Sbjct: 3 RVDLSIFIPDSLTAETGDLKIKT-YKVVLIARAASIFGVKRIVIYHDDADGEARFIRDIL 61 Query: 60 LHRSLHGTTRSVVNNMVSGVTEGFSKSLELVGVGYRAS--KSGDKIVLN--VGYSHPVEI 115 + R V ++ + + L + Sbjct: 62 TYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVD 121 Query: 116 TPEAGIEFEVPANTKIIVKGIDKERVGAYAAKIRSV-----REPEPYK 158 + + + I Sbjct: 122 IGADKLALCREKL-TVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVL 168 >MTH194_2 [E] COG0069 Glutamate synthase domain 2 Length = 252 Score = 75.1 bits (183), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 6/168 (3%), Positives = 16/168 (9%), Gaps = 12/168 (7%) Query: 1 MSRIGRKPIAVPSGVDVTLNNAVITVKGPKGTLTREL-HKDMKVTVENNEITVVRPSDNK 59 + V +L T + Sbjct: 3 DWNPSLYLHFSAERSRPAVELLA-RVPLENVEYVADLGCGPGNSTALLQQRWPAARITGI 61 Query: 60 LHRSLHGTTRSVVNNMVSGVTEGFSKSLELVGVGYRAS--KSGDKIVLN--VGYSHPVEI 115 V + + + + + Sbjct: 62 DSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLN 121 Query: 116 TPEAGIEFEVPANTKIIVKGIDKERVGAYAAKIRSV-----REPEPYK 158 + +E R Sbjct: 122 PQGVLAVQMPDNW-LEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDI 168 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.133 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,881,239 Number of extensions: 587317 Number of successful extensions: 889 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 866 Number of HSP's successfully gapped: 14 Length of query: 180 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 80 Effective length of database: 26,845,031 Effective search space: 2147602480 Effective search space used: 2147602480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 112 (47.8 bits)