BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0048.p (165 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1483 [P] COG2076 Membrane transporters of cations and cationi... 159 2e-39 MTH238 [C] COG2218 Formylmethanofuran dehydrogenase subunit C 92 3e-19 MTH234 [S] COG0599 Uncharacterized homolog of gamma-carboxymucon... 92 3e-19 MTH237 [H] COG1429 Cobalamin biosynthesis protein CobN and relat... 92 3e-19 YGL123w [J] COG0098 Ribosomal protein S5 84 1e-16 >STM1483 [P] COG2076 Membrane transporters of cations and cationic drugs Length = 109 Score = 159 bits (401), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 0/145 (0%), Positives = 0/145 (0%) Query: 19 RVAKVVKGGRRFSFSALVVVGDGNGYVGAGIGKAGEVPDAIRKGIEDAKKNLIHIPIVGT 78 + K G RR + L + + G Sbjct: 24 VLLKFSDGFRRKCYGILSLAAVLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWVL 83 Query: 79 SIPHLVTGHFGAGRVLLKPASEGTGVIAGGPVRAVLELAGVGDILTKSLGSSNSMNMVNA 138 Sbjct: 84 143 Query: 139 TLEGLSRLKRIEEVAKLRGKSVEEL 163 Sbjct: 144 168 >MTH238 [C] COG2218 Formylmethanofuran dehydrogenase subunit C Length = 337 Score = 92.4 bits (228), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 1/163 (0%), Positives = 16/163 (9%), Gaps = 11/163 (6%) Query: 3 IDPNSLELTERVVHINRVAKVVKGGRRFSFSALVVVGDGNGYVGAGIGKAGEVPDAIRKG 62 + N + ++ + + + + + + Sbjct: 44 YKAGMTHVMMVDNQKNSPTEGMEVSTPVTILEVPPLTVMAVRTYEKTSRGLKTLGEVLAT 103 Query: 63 IEDAKKNLIHI--------PIVGT--SIPHLVTGHFGAGRVLLKPASEGTGVIAGGPVRA 112 + Sbjct: 104 ETKDDLRRKLTPPADDYDQEAAIEKIRSNMEYVADVRVIVHTNPRLASVPKKKPEVFECG 163 Query: 113 VLELAGVGDILTKSLGSSN-SMNMVNATLEGLSRLKRIEEVAK 154 + + + + Sbjct: 164 LGGKTPEEKFEYALEILGKDVRASEIFSEGAFVDAIAVTKGKG 206 >MTH234 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 337 Score = 92.4 bits (228), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 1/163 (0%), Positives = 16/163 (9%), Gaps = 11/163 (6%) Query: 3 IDPNSLELTERVVHINRVAKVVKGGRRFSFSALVVVGDGNGYVGAGIGKAGEVPDAIRKG 62 + N + ++ + + + + + + Sbjct: 44 YKAGMTHVMMVDNQKNSPTEGMEVSTPVTILEVPPLTVMAVRTYEKTSRGLKTLGEVLAT 103 Query: 63 IEDAKKNLIHI--------PIVGT--SIPHLVTGHFGAGRVLLKPASEGTGVIAGGPVRA 112 + Sbjct: 104 ETKDDLRRKLTPPADDYDQEAAIEKIRSNMEYVADVRVIVHTNPRLASVPKKKPEVFECG 163 Query: 113 VLELAGVGDILTKSLGSSN-SMNMVNATLEGLSRLKRIEEVAK 154 + + + + Sbjct: 164 LGGKTPEEKFEYALEILGKDVRASEIFSEGAFVDAIAVTKGKG 206 >MTH237 [H] COG1429 Cobalamin biosynthesis protein CobN and related Mg-chelatases Length = 346 Score = 92.4 bits (228), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 15/163 (9%), Positives = 29/163 (17%), Gaps = 11/163 (6%) Query: 3 IDPNSLELTERVVHINRVAKVVKGGRRFSFSALVVVGDGNGYVGAGIGKAGEVPDAIRKG 62 I+ + V + + + Sbjct: 44 INLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQ 103 Query: 63 IEDAKKNLIHI--------PIVGT--SIPHLVTGHFGAGRVLLKPASEGTGVIAGGPVRA 112 D K + + G++ V + A Sbjct: 104 ALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDA 163 Query: 113 VLELAGVGDILTKSLGSSN-SMNMVNATLEGLSRLKRIEEVAK 154 + E G I + + M L L RI + Sbjct: 164 LAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQN 206 >YGL123w [J] COG0098 Ribosomal protein S5 Length = 254 Score = 83.6 bits (205), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 56/132 (42%), Positives = 80/132 (60%), Gaps = 8/132 (6%) Query: 10 LTERVVHINRVAKVVKGGRRFSFSALVVVGDGNGYVGAGIGKAGEVPDAIRKGIEDAKKN 69 L + V++I V K + G+R F A+VVVGD NG+VG GI A EV AIR GI AK + Sbjct: 76 LQDEVMNIKPVQKQTRAGQRTRFKAVVVVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLS 135 Query: 70 LIHIP--IVGTSI--PHLV----TGHFGAGRVLLKPASEGTGVIAGGPVRAVLELAGVGD 121 +I I GT++ PH + TG G+ V L PA G+G++A V+ +L+LAGV D Sbjct: 136 VIPIRRGYWGTNLGQPHSLATKTTGKCGSVTVRLIPAPRGSGIVASPAVKKLLQLAGVED 195 Query: 122 ILTKSLGSSNSM 133 + T+S G + ++ Sbjct: 196 VYTQSNGKTRTL 207 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.138 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,712,491 Number of extensions: 577718 Number of successful extensions: 1556 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 1549 Number of HSP's successfully gapped: 8 Length of query: 165 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 67 Effective length of database: 27,075,699 Effective search space: 1814071833 Effective search space used: 1814071833 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)