BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0012.p (230 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_cysH [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfo... 216 2e-56 BS_yitB [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfo... 216 2e-56 Rv2392 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfot... 206 2e-53 MT2462 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfot... 206 2e-53 Cgl2755 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfo... 190 2e-48 >BS_cysH [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes Length = 346 Score = 216 bits (550), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 15/208 (7%), Positives = 34/208 (16%), Gaps = 2/208 (0%) Query: 25 IIRWAVETFPN-ITFACSFGAEDVVLVDMLQKVSPSTDVFYLDTDFHFKETYETRDIMAD 83 A A DV + + + + + ++ Sbjct: 26 QNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIA 85 Query: 84 KY-GMEFVRVSPQITPAEQAVQYGEALWSTDPNQCCAIRKVEPLTRILSQYDAWITGIRR 142 I P + + D + ++ Q G Sbjct: 86 SKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG 145 Query: 143 DQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYIRENDVVYNPLHDRNFPSIGCEQ 202 A A + + + P I Q Sbjct: 146 YSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQ 205 Query: 203 CTRAVMPGEDPRAGRWSGSDKTECGLHK 230 +++ + L Sbjct: 206 NPYSLLNRSFEVGLAEVSQYEGVELLAY 233 >BS_yitB [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes Length = 346 Score = 216 bits (550), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 11/197 (5%), Positives = 30/197 (15%), Gaps = 7/197 (3%) Query: 38 FACSFGAEDVVLVDMLQKVSPSTDVFYLDTDFHFKETYETRDIMADKY-GMEFVRVSPQI 96 + + + + + + I M V P+ Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 97 TPAEQAVQYGEALWSTDPNQCCAIRKVEPLTRILSQYDAWITGIRRDQAPTRANAKKVEY 156 + + KV +R + + R + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 157 DNKFGLVKFNPIANWTSEDVWSYIRENDVVYNPLHDRNFPSIGCEQCTRAVMPGEDP--- 213 + + + + + E Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQR---AGKIRYIGV 178 Query: 214 RAGRWSGSDKTECGLHK 230 G + K Sbjct: 179 SNETAFGVMRYLHLADK 195 >Rv2392 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes Length = 346 Score = 206 bits (525), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 11/232 (4%), Positives = 30/232 (12%), Gaps = 16/232 (6%) Query: 14 AEELEHATPEEIIRWAVETF----------------PNITFACSFGAEDVVLVDMLQKVS 57 E + + P + Sbjct: 21 MTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSRE 80 Query: 58 PSTDVFYLDTDFHFKETYETRDIMADKYGMEFVRVSPQITPAEQAVQYGEALWSTDPNQC 117 + + D D+ + + + W P C Sbjct: 81 KLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNC 140 Query: 118 CAIRKVEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVW 177 + + + V + Sbjct: 141 FGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPR 200 Query: 178 SYIRENDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLH 229 +N + + ++ G +G Sbjct: 201 IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 >MT2462 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes Length = 346 Score = 206 bits (525), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 11/232 (4%), Positives = 30/232 (12%), Gaps = 16/232 (6%) Query: 14 AEELEHATPEEIIRWAVETF----------------PNITFACSFGAEDVVLVDMLQKVS 57 E + + P + Sbjct: 21 MTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSRE 80 Query: 58 PSTDVFYLDTDFHFKETYETRDIMADKYGMEFVRVSPQITPAEQAVQYGEALWSTDPNQC 117 + + D D+ + + + W P C Sbjct: 81 KLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNC 140 Query: 118 CAIRKVEPLTRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVW 177 + + + V + Sbjct: 141 FGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPR 200 Query: 178 SYIRENDVVYNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLH 229 +N + + ++ G +G Sbjct: 201 IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 >Cgl2755 [EH] COG0175 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes Length = 346 Score = 190 bits (482), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 15/223 (6%), Positives = 36/223 (16%), Gaps = 2/223 (0%) Query: 7 EDLIRVKAEELEHATPEEIIRWAVETFPNITFACSFGAEDVVLVDMLQKVSPSTDVFYLD 66 + E T + + KVS + Sbjct: 40 VAQGINLIDVAEMYPVPPRPETQGLTETY-VGNWLAKHGSREKLIIASKVSGPSRNNDKG 98 Query: 67 TDFHFKETYETRDIMADKYGMEFVRVSPQITPAEQAVQYGEALWSTDPNQCCAIRKVEPL 126 + + + + + V L Sbjct: 99 IRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLL 158 Query: 127 TRILSQYDAWITGIRRDQAPTRANAKKVEYDNKFGLVKFNPIANWTSEDVWSYIRENDVV 186 + + + G R + A V K + T ++ +S + + V Sbjct: 159 DTLDALAEYQRAGKIRYIGVSNETAFGVMRY-LHLADKHDLPRIVTIQNPYSLLNRSFEV 217 Query: 187 YNPLHDRNFPSIGCEQCTRAVMPGEDPRAGRWSGSDKTECGLH 229 + + Sbjct: 218 GLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFS 260 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,158,490 Number of extensions: 711784 Number of successful extensions: 9072 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 9006 Number of HSP's successfully gapped: 46 Length of query: 230 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 127 Effective length of database: 26,499,029 Effective search space: 3365376683 Effective search space used: 3365376683 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)