BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0021.p (1036 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yojI [V] COG0534 Na+-driven multidrug efflux pump 236 1e-61 SA2230 [V] COG1680 Beta-lactamase class C and other penicillin b... 217 1e-55 BH0715 [V] COG1680 Beta-lactamase class C and other penicillin b... 151 5e-36 alr0153 [V] COG1680 Beta-lactamase class C and other penicillin ... 111 6e-24 MA1345 [V] COG1680 Beta-lactamase class C and other penicillin b... 107 1e-22 >BS_yojI [V] COG0534 Na+-driven multidrug efflux pump Length = 452 Score = 236 bits (602), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 0/468 (0%), Positives = 0/468 (0%), Gaps = 29/468 (6%) Query: 567 LITLTNEMVFQKRTDLIDKILRSSYQNIETIENERIYSVLNNDTETISGVTNILIFGVTS 626 + + +K+ + +L++ Y + D +I + + Sbjct: 72 TPIVAQLLGAEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRLNLDIHVHQIAK 131 Query: 627 LVTLLCCFVYLGTINLLGLLIS-IAVILFAAGLYFLAGRHANQVWGETRDIQNTFFKFIN 685 L ++ + F Sbjct: 132 HFLGFLSLGIFPLFVYTVLRSFIDSLGKTRVTMMITLSSLPINF-VLNYVFIFGKFGMPA 190 Query: 686 HMVSGFKELSLHKGKKEE-FQQELKQSCDTYRIKRIQGD------LSFANVFVMGELLFT 738 G S + + I + N+ +G Sbjct: 191 LGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKNLLKIG----- 245 Query: 739 LVIGVVAFIFPLLFKDISNSSLRAYIFVFLYMTGPVHGVLDAIPNFIRVRISWNRLNELS 798 + A F ++ + ++ + + +S + Sbjct: 246 -LPIGFAVFFETSIFAAVTLL-MSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTIVVG 303 Query: 799 RQLDIAEERQEGPPDNEWSEAAPVHLVAKDITYHYQSQEGEQFAVGPLNLSVRSGQVTFV 858 +S + + + + + + Sbjct: 304 ----FEAGAARFKDARSYSLIGIMMA--IGFSLFTAACI---LLFREQIAGMYTSDPDVL 354 Query: 859 TGGNGSGKSTLGKLITGLYKPDQGEILLNGRQAA---PEELSQSFSAIFSDFHLFERLYG 915 L ++ L + + ++ Sbjct: 355 RLTQHFLIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWVIGLPVGYMVGTFTS 414 Query: 916 METGDKS-QEIQEYLQKLDIEHKVQIQQGAFSTVNLSTGQRKRLALLISCLEDRPIYLFD 974 + Sbjct: 415 LGAFGYWI 474 Query: 975 EWAADQDPEFRDYFYHVLIPELKQKGKCIIAITHDDRYFHMADQLLKM 1022 Sbjct: 475 522 >SA2230 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 217 bits (552), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 0/463 (0%), Positives = 0/463 (0%), Gaps = 20/463 (4%) Query: 32 LSNEETASIEAWINKNMREGKIPGASVVIVKGEQTVYSKGFGDSDVAAKRPVTPETLFEL 91 +++ + R + D + + L Sbjct: 35 VADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQAL 94 Query: 92 GSTSKAFTALAVLSLEKQGLLHMKDPVQKYLSWFQVSYAGEDGSGTSRVAGITLEQLLHH 151 + + + + + + Q+ + + L + + Sbjct: 95 DLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPT-------HVLMREVAW 147 Query: 152 TSGLSFDTISDIPVGGDEQALERTVKAVVGAKLDFYPGERFQYASINYDILGLVIEKVTG 211 + + V Y + + +I + + Sbjct: 148 EQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQ 207 Query: 212 ESFEVYLKNNVLNPLGLKNTYLFRTEAEQHEMARGYKLGFLKAREYQAPVYRGNTPAGYV 271 Sbjct: 208 267 Query: 272 ISNGNDMAAWLKIQMGGQAEAAVAADWIGRSHQPDRSVFPGLDGSSYAAGWFVYQKGSGE 331 Sbjct: 268 ---- 323 Query: 332 LSHGGSNPNYSSSVVFRPEEKIGVAVLANINSSYTQAMGQGIMEILHNQKLPENVS---D 388 Sbjct: 324 383 Query: 389 QYRSVDKISTVILCIAVPLILLTGWFFIMTIKEIITKERRLRRKTAKNIYXXX-XXXXXX 447 Sbjct: 384 ----- 438 Query: 448 XXXSYCFYNIPSVLFSGLSWELVEVWAPSSFMIAIPGLLIGVF 490 Sbjct: 439 481 >BH0715 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 151 bits (381), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 0/491 (0%), Positives = 0/491 (0%), Gaps = 69/491 (14%) Query: 38 ASIEAWINKNMREGKIPGASVVIVKGEQTVYSKGFGDSDVAAKRPVTPETLFELGSTSKA 97 A + + S ++ A + + + Sbjct: 32 VEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAE-ARSALPDCQFVEADIRNWQP 90 Query: 98 FTALAVLSLEKQGLLHMKDPVQKYLSWFQVSYAGEDGSGTSRVAGITLEQLLHHTSGLSF 157 AL ++ + + + L T L Sbjct: 91 VQALDLIFANASLQWLPDHYELFPHLVSLL----NPQGVLAV---QMPDNWLEPTHVLMR 143 Query: 158 DTISDIPVGG--DEQALERTVKAVVGAKLDFYPGERFQYASINYDILGLVIEKVTGESFE 215 + + E + ++ +I+ VT Sbjct: 144 EVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLR 203 Query: 216 VYLKNNVLNPLGLKNTYLFRTEAEQHEMARGYKLGFLKAREYQAPVYRGNTPAGYVISNG 275 Sbjct: 204 263 Query: 276 NDMAAWLKIQMGGQAEAAVAADWIGRSHQPDRSVFPGLDGSSYAAGWFVYQKGSGE--LS 333 Sbjct: 264 --- 320 Query: 334 HGGSNPNYSSSVVFRPEEKIGVAVLANINSSYTQA----MGQGIMEILHNQKLPENVSDQ 389 Sbjct: 321 - 379 Query: 390 YRSVDKISTVILCIAVPLILLTGWFFIMTIKEIITKERRLRRKTAKNIYXXXXXXXXXXX 449 Sbjct: 380 ------------------------- 414 Query: 450 XSYCFYNIPSVLFSGLSWELVEVWAPSSFMIAIPGLLIGVFFFSVY--YFTTSLF-PKAR 506 Sbjct: 415 --------------------- 453 Query: 507 DRTLFPIILLS 517 Sbjct: 454 464 >alr0153 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 111 bits (277), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 0/405 (0%), Positives = 0/405 (0%), Gaps = 67/405 (16%) Query: 54 PGASVVIVKGEQTVYSKGFGDSDVAAKRPVTPETLFELGSTSKAFTALAVLSLEKQGLLH 113 + V ++ + V G G + A G S S Sbjct: 22 ELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFV 81 Query: 114 MKDPVQKYLSWF-QVSYAGEDGSGTSRVAGITLEQLLHHTSGLSFDTISDIPVGGDEQAL 172 D + +A Q L L + + A+ Sbjct: 82 EADIRNWQPVQALDLIFA------------NASLQWLPDHYELF-PHLVSLLNPQGVLAV 128 Query: 173 ERTVKAVVG-----AKLDFYPGERFQYASINYDILGLVIEKVTGESFEVYLKNNVLNPLG 227 + + ++ + + + + + + Sbjct: 129 QMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMP 188 Query: 228 LKNTYLFRTEAEQ--HEMARGYKLGFLKAREYQAPVYRGNTPAGYVISNG------NDMA 279 + + ++ +A Sbjct: 189 SHQAI-IDWVTATGLRPWLQDLTES-------EQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 280 AWLK----IQMGGQAEAAVAADWIGRSHQPDRSVFPGLDGS----SYAAGWFV-YQKGSG 330 Sbjct: 241 FPRLFIVA----------- 289 Query: 331 ELSHGGSNPNYSSSVVFRPEEKIGVAVLANINSSYTQAMGQGIMEILHNQKLPENVSDQY 390 Sbjct: 290 ----- 344 Query: 391 RSVDKISTVILCIAVPLILL---TGWFFIM---TIKEIITKERRL 429 Sbjct: 345 - 388 >MA1345 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 107 bits (267), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 0/369 (0%), Positives = 0/369 (0%), Gaps = 38/369 (10%) Query: 22 AWAEPAAPNSLSNE----ETASIEAW----INKNMREGKIPGASVVIVKGEQTVYSKGFG 73 A + P + EA + + V + S + Sbjct: 47 ALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWL 106 Query: 74 DSDVAAKRPVT-PETLFELGSTSKAFTALAVLSLEKQGLLHMKDPVQKYLSWFQVSYAGE 132 + + + L + + + ++ + Sbjct: 107 PDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGV---- 162 Query: 133 DGSGTSRVAGITLEQLLHHTSGLSFDTISD---IPVGGDEQALERTVKAVVGAKLDFYPG 189 + ++ ++ + R + Sbjct: 163 ----HAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQ-QLF 217 Query: 190 ERFQYASINYDILGLVIEKVTGESFEVYLKNNVLNPLGLKNTYLFRTEAEQ--HEMARG- 246 Sbjct: 218 277 Query: 247 -YKLGFLKAREYQAPVYRGNTPAGYVISNGNDMAAWL--KIQMGGQAEAAVAADWIGRSH 303 Sbjct: 278 ----- 332 Query: 304 QPDRSVF---PGLDGSSYAAGWF-VYQKGSGELSHGGSNPNYSSSVVFRPEEKIGVAVLA 359 Sbjct: 333 -- 390 Query: 360 NINSSYTQA 368 Sbjct: 391 399 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 72,774,708 Number of extensions: 3084308 Number of successful extensions: 23018 Number of sequences better than 1.0e-05: 1092 Number of HSP's gapped: 22326 Number of HSP's successfully gapped: 1102 Length of query: 1036 Length of database: 38,378,431 Length adjustment: 117 Effective length of query: 919 Effective length of database: 24,884,353 Effective search space: 22868720407 Effective search space used: 22868720407 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 121 (51.2 bits)