BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0014.p (150 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfkN_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodieste... 101 4e-22 mlr0366 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 95 4e-20 AGc2233 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 94 8e-20 SMc04449 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiester... 92 2e-19 VC2416 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 89 2e-18 >BS_yfkN_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 651 Score = 101 bits (252), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 56/159 (35%), Positives = 89/159 (55%), Gaps = 14/159 (8%) Query: 1 VYRIYPFDNVLYVVTVTGQELRAYMEVSATHFNQWQP--GDREITANPEVPSYLYDMFAG 58 V +Y +DN + +V +TG E++ ++E+SA FNQ P G + N SY +D+ G Sbjct: 452 VGDLYLYDNTVQIVKLTGSEVKDWLEMSAGQFNQIDPAKGGDQALLNENFRSYNFDVIDG 511 Query: 59 IDYQIDLSQPAGH------------RIINVLYQGQPLADTQQLQLAVNNYRYSSLLKASK 106 + YQ+D+++PA + RIIN+ Y+G+P++ +Q+ + NNYR S Sbjct: 512 VTYQVDVTKPAKYNENGKVINADSSRIINLSYEGKPISPSQEFLVVTNNYRASGGGGFPH 571 Query: 107 LVSRAKHWESSCSVRDMLVSYIKEQGTLSPEVDNNWSIV 145 L S S+ R +L+ YI EQ T++P+ DNNWSI Sbjct: 572 LTSDKIVHGSAVENRQVLMDYIIEQKTVNPKADNNWSIA 610 >mlr0366 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 301 Score = 95.1 bits (235), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 0/162 (0%), Positives = 0/162 (0%), Gaps = 23/162 (14%) Query: 1 VYRIYPFDNVLYVVTVTGQELRAYMEVSATHFNQWQPGDRE-ITANPEVPSYLYDMFAGI 59 Sbjct: 458 517 Query: 60 DYQIDLSQP------------AGHRIINVLYQGQPLADTQQLQLAVNNYRYSSL-----L 102 Sbjct: 518 577 Query: 103 KASKLVSRAKHWESSCSVRDMLVSYIKEQGTLSPEVDNNWSI 144 Sbjct: 578 ----- 614 >AGc2233 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 301 Score = 94.0 bits (232), Expect = 8e-20, Method: Composition-based stats. Identities = 0/161 (0%), Positives = 0/161 (0%), Gaps = 13/161 (8%) Query: 1 VYRIYPFDNVLYVVTVTGQELRAYMEVSATHFNQWQPGDREITA-NPEVPSYLYDMFAGI 59 Sbjct: 461 520 Query: 60 DYQIDLSQPA------------GHRIINVLYQGQPLADTQQLQLAVNNYRYSSLLKASKL 107 Sbjct: 521 580 Query: 108 VSRAKHWESSCSVRDMLVSYIKEQGTLSPEVDNNWSIVGIG 148 Sbjct: 581 621 >SMc04449 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 657 Score = 92.4 bits (228), Expect = 2e-19, Method: Composition-based stats. Identities = 52/156 (33%), Positives = 85/156 (54%), Gaps = 13/156 (8%) Query: 1 VYRIYPFDNVLYVVTVTGQELRAYMEVSATHFNQWQPGDREIT-ANPEVPSYLYDMFAGI 59 V +Y + N + V +TG+E+R ++E+SA FN+ PG + + N + PSY +D+ G+ Sbjct: 456 VADLYLYPNTVQAVVITGEEVRNWLEMSAGIFNRIAPGSVDASLINGDFPSYNFDVIDGV 515 Query: 60 DYQIDLSQPAGH------------RIINVLYQGQPLADTQQLQLAVNNYRYSSLLKASKL 107 YQI LSQPA + RI ++ + G+P+ Q+ +A NNYR + Sbjct: 516 TYQIVLSQPAKYDKDGNVANPEASRIRDLKFDGRPIDPKQRFVVATNNYRAGGGGNFPGI 575 Query: 108 VSRAKHWESSCSVRDMLVSYIKEQGTLSPEVDNNWS 143 + + + RD++V YI EQGT++P D NW+ Sbjct: 576 AGDKVVFVAPDTNRDVIVRYIVEQGTINPSADANWT 611 >VC2416 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 634 Score = 89.4 bits (220), Expect = 2e-18, Method: Composition-based stats. Identities = 51/151 (33%), Positives = 82/151 (54%), Gaps = 16/151 (10%) Query: 13 VVTVTGQELRAYMEVSATHFNQWQPGDREITANPEVPSYLY---DMFAGIDYQIDLSQPA 69 V +TG+EL+ YME SA +FN +PGD + NPE + Y D FAG+ Y IDL+QPA Sbjct: 402 VYRMTGKELKQYMEWSAGYFNSVKPGDVTYSFNPERRASKYSTNDFFAGVTYTIDLTQPA 461 Query: 70 GHRIINVLY-QGQPLADTQQLQLAVNNYRYSSLLKASKLV----------SRAKHWESSC 118 G RI ++ + G P+ D ++L +N+YR L K ++ + A++ E Sbjct: 462 GERITDLKFADGTPVKDNSXIRLGMNSYRMGHLTKKGGILEGQQFPVLFDTEAEYGEEQG 521 Query: 119 SVRDMLVSYIKE--QGTLSPEVDNNWSIVGI 147 ++R++ + Y+ E QG + W + G+ Sbjct: 522 TIRNLTIRYLTEQKQGKYEGKPMQRWKLSGL 552 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,499,928 Number of extensions: 434542 Number of successful extensions: 1950 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 1908 Number of HSP's successfully gapped: 20 Length of query: 150 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 53 Effective length of database: 27,191,033 Effective search space: 1441124749 Effective search space used: 1441124749 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)