BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0013.p (392 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0353 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 137 3e-32 BS_yfkN_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodieste... 134 2e-31 CAC1963 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiestera... 134 2e-31 PM2014 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 127 4e-29 DR1736 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesteras... 126 5e-29 >CAC0353 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 1193 Score = 137 bits (346), Expect = 3e-32, Method: Composition-based stats. Identities = 103/312 (33%), Positives = 155/312 (49%), Gaps = 33/312 (10%) Query: 3 IQAGTSKLLNIVMIATSDLHGNLWGYRYEDGHETTN-DGMARVAAYVREVRDSGAEVILI 61 + + T I ++ATSD+HGN+ Y Y G TN G+A+V++YV VR S V+LI Sbjct: 532 VNSATQIPSTISILATSDIHGNVLNYDYATGKAPTNTQGLAKVSSYVNSVRASKPNVMLI 591 Query: 62 DNGDVFQGNMLTDDIYNKRPD--SIHPVSAALNAMGYAAMTLGNHEFNFGLG----LIEK 115 D+GD QG L+ Y + D S +P+ + AM Y TLGNHEFN+GL +I Sbjct: 592 DDGDTIQGTPLS--YYYDKIDTTSEYPLMKIMGAMKYDTWTLGNHEFNYGLPTLNRVISD 649 Query: 116 FRQELEFPVLAANAAYHSGERFAEPYTL--MEIQG--VRIAVIGLTNPNIPRW-DAGKVD 170 R+E VL+AN F PY + I G V + ++GLT IP W D Sbjct: 650 ARKE-NINVLSANTYNSDNTNFVNPYYIKTFNINGKTVNVGILGLTTKCIPDWEDPSHYA 708 Query: 171 ALHFGPMAETAQQITASLRAEGKADIIVISAHAGMVAEFDEEGGSDS-----AERITKLV 225 LHF + + A+ ++A G A++++++AH+G EEG +D+ + I V Sbjct: 709 GLHFNDLVDEAKLWVPRMKAAG-ANVVIVAAHSG------EEGAADTIPENQIKAIATNV 761 Query: 226 PEADVLLVGHMHITV-----KERIGDTV-IGGPRDRGREVVRFDLTVQLDGARPRVVNKE 279 D ++ GH H+TV K G TV + P G V + D+T+ G + + Sbjct: 762 SGIDAIVAGHAHVTVNDTSLKNPNGKTVPVVEPNKWGTFVSQIDITLDNSGNASTINTQN 821 Query: 280 VSIVDMADWEPD 291 V + D +P+ Sbjct: 822 VKMTDSIPEDPN 833 Score = 80.1 bits (196), Expect = 6e-15, Method: Composition-based stats. Identities = 70/281 (24%), Positives = 124/281 (44%), Gaps = 38/281 (13%) Query: 2 TIQAGTSKLLNIVMIATSDLHGNLWGYRYEDGHETTNDGMARVAAYVREVRDSGAEVILI 61 + A K ++V I +D HG L ED N + + V+ V S + I+ Sbjct: 32 SFAASNDKTFDLVEI--NDFHGAL-----EDTSNPANPVASVLGNRVKAVSASNPDTIVF 84 Query: 62 DNGDVFQGNMLTDDIYNKRPDSIHPVSAALNAMGYAAMTLGNHEFNFGLG-LIEKFRQEL 120 GD++QG+ L++ + PV ++++G TLGNHEF++GL L Q Sbjct: 85 GGGDLYQGSALSNILKGV------PVQKVMDSLGMQFTTLGNHEFDWGLDTLTNTTMQGA 138 Query: 121 EFPVLAANAAYHS-----GERFAEPYTLMEIQGVRIAVI-GLTN--PNI--PRWDAGKVD 170 + ++ +N + G R +PY ++ GVRIA I G+TN PNI P + + K+ Sbjct: 139 NYNIICSNLYNKNPDGSKGSRVFDPYKIITKDGVRIAFIGGITNETPNIVTPSYVSDKI- 197 Query: 171 ALHFGPMAETAQQITASLRAEGKADIIVISAHAGMVAEFDEEGGSDSAERITKLVPEADV 230 F +A + + ++ KAD+I+ H G D+ + I + + Sbjct: 198 ---FTDLATEINSVASDIKNNNKADVIIAVVHE----------GEDNLDNIVSKLSNVNA 244 Query: 231 LLVGHMHITVKERIGDTVIGGPRDRGREVVRFDLTVQLDGA 271 + GH + ++ + + + G+ +TV DG+ Sbjct: 245 VFGGHTNTIEEKLVNNIPVINANCNGKGFADLKMTVAADGS 285 >BS_yfkN_1 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 651 Score = 134 bits (338), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 75/243 (30%), Positives = 125/243 (51%), Gaps = 17/243 (6%) Query: 11 LNIVMIATSDLHGNLWGYRYEDGHETTNDGMARVAAYVREVRDSGAEVILIDNGDVFQGN 70 +++ ++AT+D+H N+ Y Y ET + G+AR A +++ R+ +L+DNGD+ QGN Sbjct: 43 VHLSILATTDIHANMMDYDYYSDKETADFGLARTAQLIQKHREQNPNTLLVDNGDLIQGN 102 Query: 71 MLTDDIYNKRPDSI------HPVSAALNAMGYAAMTLGNHEFNFGLGLIEKFRQELEFPV 124 L + + D I HP+ + +NA+ Y A TLGNHEFN+GL ++ + +FP+ Sbjct: 103 PLGEYAVKYQKDDIISGTKTHPIISVMNALKYDAGTLGNHEFNYGLDFLDGTIKGADFPI 162 Query: 125 LAANAAYHSGERFAEPYTLMEI---------QGVRIAVIGLTNPNIPRWDAGKVDA-LHF 174 + AN SGE PY + E Q V++ IG P I WD ++ + Sbjct: 163 VNANVKTTSGENRYTPYVINEKTLIDENGNEQKVKVGYIGFVPPQIMTWDKKNLEGQVQV 222 Query: 175 GPMAETAQQITASLRAEGKADIIVISAHAGMVAEFDEEGGSDSAERITKLVPEADVLLVG 234 + E+A + ++AEG AD+I+ AH G+ + G ++ + D ++ G Sbjct: 223 QDIVESANETIPKMKAEG-ADVIIALAHTGIEKQAQSSGAENAVFDLATKTKGIDAIISG 281 Query: 235 HMH 237 H H Sbjct: 282 HQH 284 >CAC1963 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 525 Score = 134 bits (338), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 90/307 (29%), Positives = 154/307 (50%), Gaps = 13/307 (4%) Query: 8 SKLLNIVMIATSDLHGNLWGYRYEDGHETTNDGMARVAAYVREVRDSGAEVILIDNGDVF 67 ++L +IV++ TSD+HGN++ Y D E G+ +++ V+ R +++ILIDNGD F Sbjct: 3 NELEHIVILETSDVHGNIFPVNYADNSEEQF-GLVKLSTIVKNERKRNSKLILIDNGDTF 61 Query: 68 QGNMLTDDIYNKRPDSIHPVSAALNAMGYAAMTLGNHEFNFGLGLIEKFRQELEFPVLAA 127 QG +T +PV LN M Y A +GNHEFN+G+ ++ ++ +FP L+ Sbjct: 62 QGTPITYYYAKIARGFSNPVIDILNYMKYDAAVIGNHEFNYGMEILNDSIKQSKFPWLSC 121 Query: 128 NAAYHSGER--FAEPYTLMEIQG-VRIAVIGLTNPNIPRWDAGK-VDALHFGPMAETAQQ 183 N + F PY + E +G +RIA++G+T IP W+ + L F + E ++ Sbjct: 122 NIIDTKTKETAFGVPYIIKEFRGDLRIAILGVTTKYIPNWENQDIIKDLKFLDVIEETKK 181 Query: 184 ITASLRAEGKADIIVISAHAGMVAEFD------EEGGSDSAERITKLVPEADVLLVGHMH 237 L+ + K DI+V+S H G D + G + A + V DVLL GH H Sbjct: 182 WVRILKNDKKVDIVVVSYHGGFEKNIDTGEETESQTGENQAYELCNEVDGIDVLLTGHQH 241 Query: 238 ITVK-ERIGDTVIGGPRDRGREVVRFDLTVQLDGARPRVVNKEVSIVDMADWEPDPEFRR 296 + +I I P + + + D+ + R+V + S++ +++ D + + Sbjct: 242 REIYGMKINGVTIIQPGSSAKNIGKVDIIAEKTLLGWRIVESKSSLISVSNISSDYKAGK 301 Query: 297 LVAEAHE 303 +AE +E Sbjct: 302 -IAEKYE 307 >PM2014 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 656 Score = 127 bits (318), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 103/328 (31%), Positives = 149/328 (45%), Gaps = 36/328 (10%) Query: 15 MIATSDLHGNLWGYRYEDGHETTNDGMARVAAYVREVRDSGAEVILIDNGDVFQGNMLTD 74 +IAT+DLH L + Y T G R A+ +R+ R +L+DNGD+ QGN + D Sbjct: 35 IIATTDLHSFLTDFDYYKDAPTEKFGFTRAASLIRQARKEVKNSVLVDNGDLIQGNPIAD 94 Query: 75 DIYNK--RPDSIHPVSAALNAMGYAAMTLGNHEFNFGLGLIEKFRQELEFPVLAANAAY- 131 K + ++P LNAM Y TLGNHEFN+GL ++ E FP++ AN Sbjct: 95 YQAAKGYKEGKMNPAIEVLNAMKYDVGTLGNHEFNYGLKYLDDAIAEANFPIINANVVKA 154 Query: 132 HSGERFAEPYTLME---------IQGVRIAVIGLTNPNIPRWDAGKVDALHFG-PMAETA 181 SGE +PY + E +Q ++I IG P + WD ++ + +TA Sbjct: 155 DSGEPRYQPYVIQEKGVRDANGHLQMIKIGYIGFVPPQVMVWDKAHLEGHVLAHDIKKTA 214 Query: 182 QQITASLRAEGKADIIVISAHAGMVAEFDEEGGSDSAERITKLVPEADVLLVGHMH--IT 239 ++ L+A+G ADII+ AH G E +EG +SA + V D ++ GH H Sbjct: 215 EKYVPELKAKG-ADIIIALAHTGPSDEPYKEGAENSAFYLAD-VKGIDAVIFGHSHRLFP 272 Query: 240 VKERIGD--------TVIGGPRDR----GREVVRFDLTVQLDGARPRVVNKEVSI----- 282 KE TV G P G + DL + G + VVN + + Sbjct: 273 NKEFAKSPNVDIEKGTVKGVPESMAGYWGNNISVVDLELAQHGDKWVVVNGKAELRPIYD 332 Query: 283 --VDMADWEPDPEFRRLVAEAHEETMTF 308 MA +PD E L+A H+ T F Sbjct: 333 NEKKMAIAQPDDEMNALLAPVHDATRQF 360 >DR1736 [F] COG0737 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases Length = 678 Score = 126 bits (317), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 97/341 (28%), Positives = 154/341 (45%), Gaps = 44/341 (12%) Query: 9 KLLNIVMIATSDLHGNLWGYRYEDGHETTNDGMARVAAYVREVRDSGAEVILIDNGDVFQ 68 + +++ ++ T+DLH N GY Y T G+ A +++ R+ +L DNGD+ Q Sbjct: 62 QTVDLRILETTDLHTNALGYDYYQDKPTGEFGLEYTATLLKQAREEKRNTLLYDNGDLIQ 121 Query: 69 GNMLTDDIYNKRP---DSIHPVSAALNAMGYAAMTLGNHEFNFGLGLIEKFRQELEFPVL 125 G L D + +P +HP+ AA+ + Y A LGNHEFN+GL +++ P++ Sbjct: 122 GTPLGDYVARVQPLRAGQMHPLHAAMRLLKYDAANLGNHEFNYGLPFLQQVVAAAPMPIV 181 Query: 126 AANAAY-----HSGERFAEPY-----TLMEIQG----VRIAVIGLTNPNIPRWDAGKVD- 170 +AN G PY T+ + +G + + VIGL P I +WD +D Sbjct: 182 SANTYVDDGNGKPGANAFTPYLIQRRTVYDTEGRPYILNVGVIGLLTPQIVQWDKSNLDG 241 Query: 171 ALHFGPMAETAQQITASLRAEGKADIIVISAHAGMVAEFDEEGGSDSAERITKLVPEADV 230 + + +TA++ ++A G ADIIV AH+G+ A++ + G + A +TK VP DV Sbjct: 242 KIVTADIVDTARKFVPEMKAAG-ADIIVAVAHSGINADY-QPGQENVATELTK-VPGIDV 298 Query: 231 LLVGHMHITVKERIGDTVIGGPRDRGR----------------EVVRFDLTVQLDGARPR 274 +L GH H + T+ G G VV L Sbjct: 299 VLSGHSHQEFPGPVYKTIPGADITNGTINGKPVVMAGYWGNDLGVVDLQLNYDRKSQTWT 358 Query: 275 VVNKEVSIVDMADW-------EPDPEFRRLVAEAHEETMTF 308 + N + S+ + D PDP R V AHE T+ + Sbjct: 359 IQNAKTSLRPIWDKTAKKSLVTPDPAIARAVKAAHEGTLRY 399 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,635,269 Number of extensions: 1252211 Number of successful extensions: 2936 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 2813 Number of HSP's successfully gapped: 50 Length of query: 392 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 284 Effective length of database: 25,922,359 Effective search space: 7361949956 Effective search space used: 7361949956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)