BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0012.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurM [G] COG0395 ABC-type sugar transport system, permease co... 155 8e-38 BH1246 [G] COG0395 ABC-type sugar transport system, permease com... 138 1e-32 BH1119 [G] COG0395 ABC-type sugar transport system, permease com... 136 5e-32 all0261 [G] COG0395 ABC-type sugar transport system, permease co... 129 5e-30 all4824 [G] COG0395 ABC-type sugar transport system, permease co... 127 2e-29 >BS_yurM [G] COG0395 ABC-type sugar transport system, permease component Length = 300 Score = 155 bits (391), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 81/267 (30%), Positives = 135/267 (50%), Gaps = 2/267 (0%) Query: 7 NSSIYILMIIIAVLQLFPLYWLVISAFKDNSEIIGGTVWALPTEWQLSNFTEAWVSAKVN 66 +S+++ + + + +PL W+V+SAFK++ +I + W+LP+ W NF AW + ++ Sbjct: 34 ETSVWVFLFLYLIAIAYPLLWMVMSAFKNSDDIFEHS-WSLPSSWHPENFVSAW-NQGIS 91 Query: 67 QYFGNSVIXXXXXXXXXXXXASMMAYALTRMKFKYNSLILFILLMGVMVPIHATLIPLFM 126 YF NSVI ++ AY L+R +FK L + L G+M+ +L+PL+ Sbjct: 92 SYFMNSVIVTALTCVITVFISAWAAYGLSRFEFKGKGFFLVLCLGGLMLTPQVSLVPLYS 151 Query: 127 ILKNLGXXXXXXXXXXPYIAVNLPIGVYMLSAFLRTMPKELEEAACMDGCGVVKSFFXXX 186 I+++LG PY A +P + ++ ++ ++ KELEEAA +DGC FF Sbjct: 152 IIQSLGLYNTYWALILPYAAYRIPFTIILIRSYFLSISKELEEAAYLDGCTSFGVFFRIF 211 Query: 187 XXXXXXXXASVAIFVFLAVWNELLMAATFIQKQELRTLPLGLMNFSGQYSISWGPLAAAM 246 + I WNE + A FI + LRT+P GLM F WG L A + Sbjct: 212 LPMSVPILVTSGILTAYHTWNEFMFAIIFIDDENLRTIPAGLMQFRDALQTDWGVLLAGL 271 Query: 247 VISTLPILLAYVLFSDQMEKSFTAGAI 273 IS PI++ ++L + +G++ Sbjct: 272 TISAAPIIILFLLMQKYFVRGIASGSV 298 >BH1246 [G] COG0395 ABC-type sugar transport system, permease component Length = 273 Score = 138 bits (347), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 85/272 (31%), Positives = 137/272 (50%), Gaps = 1/272 (0%) Query: 2 LRKTKNSSIYILMIIIAVLQLFPLYWLVISAFKDNSEIIGGTVWALPTEWQLSNFTEAWV 61 ++K +S Y +I+ L +FP W+ +++ K EI W E ++ A Sbjct: 1 MKKKSGASFYFFLIVFVFLVMFPFIWVFLTSIKPPREIFASFAW-FSAEPTFQSYVNAVN 59 Query: 62 SAKVNQYFGNSVIXXXXXXXXXXXXASMMAYALTRMKFKYNSLILFILLMGVMVPIHATL 121 + + Y NS + AS+ AYALTR+ K LIL +L M P A + Sbjct: 60 NRPLFTYMMNSFVISSLTTLIAIACASLAAYALTRLPIKGKGLILGTVLAASMFPQIAII 119 Query: 122 IPLFMILKNLGXXXXXXXXXXPYIAVNLPIGVYMLSAFLRTMPKELEEAACMDGCGVVKS 181 P++ + +LG PYI ++LP+ +++L+ F + +P ELEE+A +DG ++ Sbjct: 120 TPIYSFITDLGLRNSYFGLVIPYITISLPLAIWILATFFKKIPFELEESAKLDGATPFQT 179 Query: 182 FFXXXXXXXXXXXASVAIFVFLAVWNELLMAATFIQKQELRTLPLGLMNFSGQYSISWGP 241 F + AI VF+A WNE L A T LRT+P+GL + QY+I WG Sbjct: 180 FRKIIFPLAAPGVFTTAILVFIAAWNEYLFALTINTADRLRTVPVGLSLYESQYTIPWGD 239 Query: 242 LAAAMVISTLPILLAYVLFSDQMEKSFTAGAI 273 + AA VI T+PI++ +LF ++ T+G++ Sbjct: 240 ITAATVIVTVPIVIIVLLFQRRIVAGLTSGSV 271 >BH1119 [G] COG0395 ABC-type sugar transport system, permease component Length = 281 Score = 136 bits (342), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 75/255 (29%), Positives = 126/255 (49%), Gaps = 4/255 (1%) Query: 11 YILMIIIAVLQLFPLYWLVISAFKDNSEIIGGTVWALPTEWQLSNFTEAWVS---AKVNQ 67 Y +I + ++P+ W+V S+ K SEI +P+E+ SN+ + W + Sbjct: 14 YGFVIAFGFIMVYPILWMVASSLKPASEIFSQASSLIPSEFVWSNYPDGWAGFGRTGFDI 73 Query: 68 YFGNSVIXXXXXXXXXXXXASMMAYALTRMKFKYNSLILFILLMGVMVPIHATLIPLFMI 127 YF NS I +S++AY R++FK+ ++ L+ VM+P+ TLIP +++ Sbjct: 74 YFKNSFIITTSVVIGAILSSSIVAYGFARLQFKFKKVLFACLIATVMLPVQITLIPQYIL 133 Query: 128 LKNLGXXXXXXXXXXPYIAVNLPIGVYMLSAFLRTMPKELEEAACMDGCGVVKSFFXXXX 187 NLG P P +++L F+R +P+EL+EAA +DGC F+ Sbjct: 134 FHNLGWVNTFYPLIVPAFLGGTPFFIFLLIQFIRGIPRELDEAAIIDGCSTFGVFWRVIL 193 Query: 188 XXXXXXXASVAIFVFLAVWNELLMAATFIQKQELRTLPLGLMNF-SGQYSISWGPLAAAM 246 +VAIF F+ W++ L ++ K +++T+ LGL NF + SWG L A Sbjct: 194 PLLKPALVTVAIFAFMWTWDDFLAPLIYLNKTDIQTVALGLRNFMDAESGTSWGALLAMS 253 Query: 247 VISTLPILLAYVLFS 261 +S LP + ++ F Sbjct: 254 TLSLLPQFIIFLFFQ 268 >all0261 [G] COG0395 ABC-type sugar transport system, permease component Length = 276 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 88/265 (33%), Positives = 131/265 (49%), Gaps = 5/265 (1%) Query: 10 IYILMIIIAVLQLFPLYWLVISAFKDNSEIIGGTVWALPTEWQLSNFTEAWVSAKVN-QY 68 +Y+L+ AV+ L P W + ++FK SEI+ GT LP + L N+ + ++ + ++ Sbjct: 12 LYVLLTTYAVITLVPFLWALSASFKPLSEIVSGTPNFLPQNFTLDNYRQIFLQEPLFLRW 71 Query: 69 FGNSVIXXXXXXXXXXXXASMMAYALTRMKFKYNSLILFILLMGVMVPIHATLIPLFMIL 128 NSV+ SM YAL R+ F + F++L + VP TLIP F+IL Sbjct: 72 LFNSVVIAVSVTLLNLLFNSMAGYALARLSFVGKNFWFFLILAVLAVPAQITLIPTFLIL 131 Query: 129 KNLGXXXXXXXXXXPYIAVNLPIGVYMLSAFLRTMPKELEEAACMDGCGVVKSFFXXXXX 188 K +G P + VN ++M+ F P+ELEEAA +DG + F Sbjct: 132 KAIGWLNSYQGMIVPSM-VNATF-IFMMRQFFINFPQELEEAAQLDGLNTIGIFRYIILP 189 Query: 189 XXXXXXASVAIFVFLAVWNELLMAATFIQKQELRTLPLGLMNFSGQYSISWGPLAAAMVI 248 A+ A+FVF+ WN L+ + + E+ TLPLGL F GQY W + AA ++ Sbjct: 190 LAKPALAAQAVFVFMGSWNNFLLPVVILFEPEMFTLPLGLNTFKGQYISYWNYIMAASMV 249 Query: 249 STLPILLAYVLFSDQMEKS--FTAG 271 TLP L Y F+ +S FT G Sbjct: 250 FTLPALSIYAFFNRYFIQSATFTGG 274 >all4824 [G] COG0395 ABC-type sugar transport system, permease component Length = 279 Score = 127 bits (318), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 81/262 (30%), Positives = 128/262 (48%), Gaps = 8/262 (3%) Query: 4 KTKNSS-----IYILMIIIAVLQLFPLYWLVISAFKDNSE-IIGGTVWALPTEWQLSNFT 57 KT+NS +Y L+ +IA + LFPL WL+ +A K +E I LP + + NF Sbjct: 4 KTQNSPWRTLVMYGLLGVIAFITLFPLLWLISTALKSPTENIFQSPPQLLPNQPTVDNFF 63 Query: 58 EAWVSAKVNQYFGNSVIXXXXXXXXXXXXASMMAYALTRMKFKYNSLILFILLMGVMVPI 117 + W S QY NS + S+ AY L R+ F I ++ +M+P Sbjct: 64 KVWNSLPFGQYLYNSTLVSVLTVGLNLLFCSLAAYPLARLSFPGRDWIFIAIVSTIMIPF 123 Query: 118 HATLIPLFMILKNLGXXXXXXXXXXPYIAVNLPIGVYMLSAFLRTMPKELEEAACMDGCG 177 +IPL+++ LG P +A G+++L +PKE+EEAA MDG Sbjct: 124 QIVMIPLYILTVQLGLRNTYLGMMFPSLASAF--GIFLLRQAFMGVPKEIEEAARMDGSS 181 Query: 178 VVKSFFXXXXXXXXXXXASVAIFVFLAVWNELLMAATFIQKQELRTLPLGLMNFSGQYSI 237 + ++ ++AIFVF+ W++ L IQ + L TLPLG+ +G +S+ Sbjct: 182 ELGLWWHIMLPAVRPALVTLAIFVFIGAWSDFLWPLIVIQDENLYTLPLGVAKLAGTFSL 241 Query: 238 SWGPLAAAMVISTLPILLAYVL 259 W +AA VI+ P+LL +++ Sbjct: 242 DWRLVAAGSVIAIAPVLLLFLI 263 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,535,813 Number of extensions: 463700 Number of successful extensions: 1961 Number of sequences better than 1.0e-05: 147 Number of HSP's gapped: 1706 Number of HSP's successfully gapped: 147 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)