BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0181.0004.p (316 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1065 [G] COG4209 ABC-type polysaccharide transport system, per... 346 2e-95 BH1911 [G] COG4209 ABC-type polysaccharide transport system, per... 224 2e-58 BH0484 [G] COG4209 ABC-type polysaccharide transport system, per... 208 1e-53 BH2112 [G] COG4209 ABC-type polysaccharide transport system, per... 186 3e-47 BS_lplB [G] COG4209 ABC-type polysaccharide transport system, pe... 185 7e-47 >BH1065 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 317 Score = 346 bits (888), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 172/319 (53%), Positives = 229/319 (71%), Gaps = 5/319 (1%) Query: 1 MEANTIQQVNPGKPLRQSRKKK--VKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDY 58 ME+ + N R+ +K+K ++ W LH++++PA V+ IF Y PM GI+MAFQDY Sbjct: 1 MESQAVTS-NAKTVERKLKKQKWNFRRTWQLHVLLIPALVLALIFQYGPMFGIVMAFQDY 59 Query: 59 KPKPGLFGSPWVGLKHFEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRK 118 KP G GS WVGL+HF M++ DY Q+ WNTL + K+I LI+P ALLLNE+ + Sbjct: 60 KPWLGFTGSEWVGLQHFITMFEYDYAKQVIWNTLVISVLKMIFGLIVPIIVALLLNEMYQ 119 Query: 119 MALKRTIQTLVYLPHFLSWVTLSGILIDMLA-QTGLINQFISSVFGIKPIFFLGDGSWFR 177 M +KRT+QT++YLP+FLSWV L GILID+L+ G++NQ + +FGI PIFFLG WFR Sbjct: 120 MVVKRTVQTIIYLPYFLSWVILGGILIDVLSPDGGIVNQAL-GLFGIDPIFFLGSNDWFR 178 Query: 178 FTIIFSDVWKEFGFNMIIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXX 237 T++ SD+WKE GFN I+FLAA++ ++P+LYEAA VDGA RWKQT++ Sbjct: 179 TTVVISDLWKETGFNTIVFLAAITAVNPNLYEAAVVDGANRWKQTLHITLPAMLPIIIVV 238 Query: 238 XXXXLGNVLNANFDQVFNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISL 297 LGN+LNA FDQ+FNLY+PL+YQ GDIIDT+VYR L++G FS+ TAV LFKS ISL Sbjct: 239 ATLSLGNILNAGFDQIFNLYNPLVYQTGDIIDTYVYRVSLVNGDFSYGTAVGLFKSLISL 298 Query: 298 ILIVISYRLAYRFAGYRIF 316 +LI+I YRLAY++A +RIF Sbjct: 299 VLIIIGYRLAYKYANWRIF 317 >BH1911 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 319 Score = 224 bits (571), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 119/317 (37%), Positives = 187/317 (58%), Gaps = 5/317 (1%) Query: 4 NTIQQVNPGKPLRQSRKK---KVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKP 60 T+++ K Q RKK +KQ L+LM LP V +F+Y P+ G MAFQ+++P Sbjct: 2 ETVKEKAHSKIRVQRRKKILTTLKQQKYLYLMSLPFVAWVLVFNYFPLWGWTMAFQNFRP 61 Query: 61 KPGLFGSPWVGLKHFEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMA 120 F WVGL+HF ++Q++ F + NTL + +I+ +P FA+LLNEVR M Sbjct: 62 GLSFFEQEWVGLQHFIELFQDNRFYLVLRNTLAMSIMGLIVGFTMPILFAVLLNEVRHMV 121 Query: 121 LKRTIQTLVYLPHFLSWVTLSGILIDMLA-QTGLINQFISSV-FGIKPIFFLGDGSWFRF 178 KRT+QT+ YLPHF+SWV ++GI+ ML+ G++NQ + + F +P+ F+ G WF Sbjct: 122 FKRTVQTVSYLPHFVSWVVVAGIVTKMLSIDGGIVNQLLMAFGFVDQPVQFMAKGEWFWV 181 Query: 179 TIIFSDVWKEFGFNMIIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXX 238 + SD+WKE G+N IIFLAA++GI P LYEAA+VDGA RW+Q + Sbjct: 182 IVTLSDLWKEMGWNSIIFLAAMAGIDPQLYEAAKVDGASRWRQIWHITLPGIRPTILVIL 241 Query: 239 XXXLGNVLNANFDQVFNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLI 298 +G+++ F++ F L +PL+ +++D + GL G++S+ TA+ +F S + +I Sbjct: 242 ILSIGHLMAIGFEKQFLLGNPLVVDYSEVLDLYALNYGLGLGRYSYGTAIGIFNSVVCII 301 Query: 299 LIVISYRLAYRFAGYRI 315 L+ ++ + RF + Sbjct: 302 LLFLANGIFKRFTNQSV 318 >BH0484 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 325 Score = 208 bits (529), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 121/314 (38%), Positives = 185/314 (58%), Gaps = 5/314 (1%) Query: 2 EANTIQQVNPGKPLRQSRKKKVKQPWM---LHLMVLPAAVMVFIFSYIPMGGILMAFQDY 58 +A I+ P P + R++K+K+ + L+LM+LP V IF Y+PM G+++AFQDY Sbjct: 8 QALPIESAAPSIPKKVRRQQKLKRIFRNKYLYLMILPGLVYFLIFKYVPMWGLIIAFQDY 67 Query: 59 KPKPGLFGSPWVGLKHFEYMWQNDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRK 118 +P G+ GS WVG KHFE ++ F Q+ NTL +I +P A+LLNE+R Sbjct: 68 QPFLGVTGSEWVGFKHFERLFTEPTFFQLLRNTLVLFLYNLIFFFPVPIILAVLLNEIRL 127 Query: 119 MALKRTIQTLVYLPHFLSWV-TLSGILIDMLAQTGLINQFISSVFGIKPIFFLGDGSWFR 177 M KR +QT++Y+PHF+SWV +S + G+IN+ I S+ G K + FL WFR Sbjct: 128 MVFKRFVQTMIYIPHFMSWVIVVSLTYVLFTTDGGVINEIIYSITG-KNVNFLMSEDWFR 186 Query: 178 FTIIFSDVWKEFGFNMIIFLAALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXX 237 I +W+E G+ II+ AA++ I PSLYEAA++DGA R++Q + Sbjct: 187 PLYILQVIWREAGWATIIYFAAIAAIDPSLYEAAKMDGANRFRQIWHITLPAIRSVIVVL 246 Query: 238 XXXXLGNVLNANFDQVFNLYSPLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISL 297 +G++L F+ VF + + + + +I DT+VY GL GQFS++TAV FKS + L Sbjct: 247 LILKIGDILELGFEHVFLMLNAMNREVAEIFDTYVYTAGLKEGQFSYSTAVGFFKSIVGL 306 Query: 298 ILIVISYRLAYRFA 311 I+++++ LA +F Sbjct: 307 IMVMLANWLAKKFG 320 >BH2112 [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 337 Score = 186 bits (473), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 106/298 (35%), Positives = 166/298 (55%), Gaps = 2/298 (0%) Query: 21 KKVKQPWMLHLMVLPAAVMVFIFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKHFEYMWQ 80 KK + L LM LP + + IF Y+P+ G +AFQD++P +F W G KHF +++ Sbjct: 40 KKCYEQKALLLMSLPFLIWLIIFRYVPLYGWSIAFQDFRPARPIFDQDWAGFKHFTFLFT 99 Query: 81 NDYFLQITWNTLFFAGTKIIMNLIIPFFFALLLNEVRKMALKRTIQTLVYLPHFLSWVTL 140 + F ++ NTL + +++ + A+LLNE+R + KR +QT+ Y+PHFLSWV Sbjct: 100 DPRFFEVLRNTLAMSLINLVLGFVTAIGLAILLNELRNLTFKRVVQTISYMPHFLSWVVA 159 Query: 141 SGILIDMLA-QTGLINQFISSVFGI-KPIFFLGDGSWFRFTIIFSDVWKEFGFNMIIFLA 198 +G++ L+ + G+IN + + I +PI +LG G +F I SDVWK G+N II+LA Sbjct: 160 AGLVSAALSIENGIINIVLMKLGMISEPIMWLGVGEYFWGIIGASDVWKNVGWNTIIYLA 219 Query: 199 ALSGISPSLYEAAEVDGAGRWKQTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQVFNLYS 258 A++ I YEAAE+DGA R ++ Y LG +L + F+ + L + Sbjct: 220 AITMIDTEQYEAAEIDGATRLQRIWYITLPGMRPVIVILLIMNLGYILESGFEAQYLLMN 279 Query: 259 PLIYQQGDIIDTFVYREGLLSGQFSFATAVNLFKSAISLILIVISYRLAYRFAGYRIF 316 P+ + +D FV R G+ G FS ATA +FK+ +S I + + +A R R+F Sbjct: 280 PMNMDFAENLDIFVLRYGINMGNFSLATAAGIFKTVVSFIFLFSANHIAKRLGEARLF 337 >BS_lplB [G] COG4209 ABC-type polysaccharide transport system, permease component Length = 318 Score = 185 bits (470), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 108/270 (40%), Positives = 157/270 (58%), Gaps = 2/270 (0%) Query: 42 IFSYIPMGGILMAFQDYKPKPGLFGSPWVGLKHFEYMWQNDYFLQITWNTLFFAGTKIIM 101 IF Y+PM G+L+AF+DY P G + S WVG +F+ + N F ++ NTL A ++ Sbjct: 44 IFKYLPMWGVLIAFKDYSPYLGFWKSEWVGFDYFKDFFMNPDFFRLLRNTLMLASLDLLF 103 Query: 102 NLIIPFFFALLLNEVRKMALKRTIQTLVYLPHFLSW-VTLSGILIDMLAQTGLINQFISS 160 P ALLLNEVRK KR IQT +Y+PHF+SW + +S + TG+IN+ I S Sbjct: 104 AFPAPLILALLLNEVRKAVYKRCIQTFIYVPHFVSWTIVVSITFVFFTVDTGVINKLIMS 163 Query: 161 VFGIKPIFFLGDGSWFRFTIIFSDVWKEFGFNMIIFLAALSGISPSLYEAAEVDGAGRWK 220 + G + I FL D WFR I+ +WKE G+ I+FLAAL+ + YEAA +DGAGR++ Sbjct: 164 LTG-EQISFLSDADWFRPMIVMQSIWKETGWGTILFLAALATVDQEQYEAAIMDGAGRFR 222 Query: 221 QTIYXXXXXXXXXXXXXXXXXLGNVLNANFDQVFNLYSPLIYQQGDIIDTFVYREGLLSG 280 + + +G+ LN F+QV+ + + L DI DT+VY G+ G Sbjct: 223 RMWHITLPAIRSTIIVLLILRIGSFLNLGFEQVYLMTNSLNRSVADIFDTYVYMMGITQG 282 Query: 281 QFSFATAVNLFKSAISLILIVISYRLAYRF 310 +S++TAV LFKS + +ILI + +A +F Sbjct: 283 AYSYSTAVGLFKSVVGIILIFGANYIAKKF 312 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.144 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,735,323 Number of extensions: 846577 Number of successful extensions: 2312 Number of sequences better than 1.0e-05: 134 Number of HSP's gapped: 2210 Number of HSP's successfully gapped: 134 Length of query: 316 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 210 Effective length of database: 26,153,027 Effective search space: 5492135670 Effective search space used: 5492135670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 116 (49.3 bits)