BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0020.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1640 [S] COG1434 Uncharacterized conserved protein 222 7e-58 TM1640 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 222 7e-58 PAB1738 [ER] COG0493 NADPH-dependent glutamate synthase beta cha... 176 5e-44 PAB1214 [ER] COG0493 NADPH-dependent glutamate synthase beta cha... 154 1e-37 PH1873 [ER] COG0493 NADPH-dependent glutamate synthase beta chai... 152 4e-37 >STM1640 [S] COG1434 Uncharacterized conserved protein Length = 266 Score = 222 bits (565), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 18/303 (5%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 V + + R + + VE + G + + + G Sbjct: 166 VTNDDTDAPRWLSFPGFVPVLRHLNDGTRFANVEEGIWTVERYLSLIAGELPRLRDDETG 225 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKTGIPALELM---------GRRVA 111 Y + P IE D Sbjct: 226 YGPRGKDFIIHVDIPRDIENAWQVLQADTTLRS-- 283 Query: 112 VIGAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRI 171 Sbjct: 284 343 Query: 172 VGDELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQ-KRRVDLIE 230 Sbjct: 344 - 402 Query: 231 ALGLE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQ 289 Sbjct: 403 ---- 458 Query: 290 MSG 292 Sbjct: 459 461 >TM1640 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 222 bits (565), Expect = 7e-58, Method: Compositional matrix adjust. Identities = 0/303 (0%), Positives = 0/303 (0%), Gaps = 18/303 (5%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 I + + V + Sbjct: 166 YDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQML 225 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKTGIPALELM---------GRRVA 111 Sbjct: 226 -- 283 Query: 112 VIGAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRI 171 Sbjct: 284 343 Query: 172 VGDELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQ-KRRVDLIE 230 Sbjct: 344 - 402 Query: 231 ALGLE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQ 289 Sbjct: 403 ---- 458 Query: 290 MSG 292 Sbjct: 459 461 >PAB1738 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 176 bits (445), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 0/301 (0%), Positives = 0/301 (0%), Gaps = 19/301 (6%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 Y + + + + L + + + ++ + Sbjct: 180 RTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILL 239 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKT----GIPALEL---MGRRVAVI 113 Sbjct: 240 299 Query: 114 GAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVG 173 Sbjct: 300 - 358 Query: 174 DELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQ---KRRVDLIE 230 Sbjct: 359 -- 416 Query: 231 ALGLEHERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQM 290 Sbjct: 417 ------ 470 Query: 291 S 291 Sbjct: 471 471 >PAB1214 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 211 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 0/300 (0%), Positives = 0/300 (0%), Gaps = 16/300 (5%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 Sbjct: 180 239 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKT----GIPALE---LMGRRVAVI 113 Sbjct: 240 299 Query: 114 GAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVG 173 Sbjct: 300 - 358 Query: 174 DELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQK-RRVDLIEAL 232 Sbjct: 359 - 417 Query: 233 GLE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQMS 291 Sbjct: 418 ----- 472 >PH1873 [ER] COG0493 NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 252 Score = 152 bits (385), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 0/300 (0%), Positives = 0/300 (0%), Gaps = 16/300 (5%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 Y + + + + L + + + ++ + Sbjct: 180 RTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILL 239 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKT----GIPALE---LMGRRVAVI 113 Sbjct: 240 299 Query: 114 GAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVG 173 Sbjct: 300 - 358 Query: 174 DELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQK-RRVDLIEAL 232 Sbjct: 359 - 417 Query: 233 GLE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQMS 291 Sbjct: 418 ----- 472 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,273,364 Number of extensions: 1005051 Number of successful extensions: 4553 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 4446 Number of HSP's successfully gapped: 70 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)