BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0018.p (526 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1994_2 [TQ] COG2508 Regulator of polyketide synthase expression 82 2e-15 BS_yunI [TQ] COG2508 Regulator of polyketide synthase expression 74 5e-13 BH0757 [TQ] COG2508 Regulator of polyketide synthase expression 65 3e-10 CAC1426 [TQ] COG2508 Regulator of polyketide synthase expression 63 1e-09 >BH1994_2 [TQ] COG2508 Regulator of polyketide synthase expression Length = 313 Score = 82.4 bits (202), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 49/147 (33%), Positives = 82/147 (55%), Gaps = 2/147 (1%) Query: 370 QLVIGVSKSHRGLKHAYAAYQEAVQALSLYSCYQKSILF--YEELGVFQLLLSLNDGKTL 427 +L++G+ + ++ YQ+AVQ ++ S + I F ++ELG + LL L + Sbjct: 167 RLLLGIGGKTETIHDYFSCYQQAVQVHNVVSHRFRDIGFALFDELGSYTLLHHLKNTPAT 226 Query: 428 ENFIRSYLGPLIDHDSAKGSELLLTLRVYLDHDGSKQIAARNLFIVRQSLYYRLDKITEL 487 FI +L PL+ + + ++L TLRVYL+ +GS + + L+I R SL YRL+KI L Sbjct: 227 SLFIDKHLQPLLQYSQGRSTDLFYTLRVYLNQNGSIKETSDELYIHRSSLLYRLEKIETL 286 Query: 488 LGEDFMLPENRISIQVALRAYQLLYPE 514 L D E+R ++ +A + Y L Y + Sbjct: 287 LDVDLNDSEHRFNLMMAFKLYDLFYTK 313 >BS_yunI [TQ] COG2508 Regulator of polyketide synthase expression Length = 531 Score = 74.3 bits (181), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 120/528 (22%), Positives = 224/528 (42%), Gaps = 45/528 (8%) Query: 1 VHVLESAGFESLIHGEEMILTTGMGASAGLPSSLS-FMQNLIDKNAACLCIELGDYFSSI 59 V+++++ +H E+++TT P LS ++ + + A L I+ Y I Sbjct: 31 VNMMDAPDIVDFLHKNELLVTTAYHLKDH-PHQLSELIRQMAKRGCAGLGIKTKRYLEDI 89 Query: 60 PQEMIDLANRHDFPLIIFPRTVRFVDITLDLHSLIINRHHRMLQELESISREFHRLTLTS 119 P+E+I+LA+ + FP+I P +R DI S I++ LQ+ ++F ++ Sbjct: 90 PKEIIELADSYAFPIIELPEHIRLGDIVNATLSHILDMRSNELQQAIYAHKKFTNHIMSG 149 Query: 120 QGTIKVLQLLCKSTRTQIVYMQPQGKPLFFPALSPEDQAPLLSFFEAFGEEMEGVQPD-- 177 +G LQ L K L P L + A +LS E E ++ Sbjct: 150 KG----LQSLLKKV----------SDILQLPVLLLDQHAKMLSASHQISVETEKLKGTLN 195 Query: 178 --AAPYIRDYG----HKTIALKPVGALDQTWAYILMVCNHKPQEFDCLL-LDSASLSIAQ 230 + P+ + KT ++ P+ ++ Y+L+ + + +L ++ A+ I+ Sbjct: 196 TVSGPFFTCFSTISDQKTYSVLPIYNHEKNCGYLLIPDMVQAGDKGLILTIEQAANVISF 255 Query: 231 ELLRTRYMEERKLFSENLWVDELIGGRTQDDNRLKGLVGPDFNVVNELPYRVCLVEIENS 290 ELL+ +++ + N + + I D+ +K +F + + Y +C+ + Sbjct: 256 ELLKENALKQFSRRARNEFFNNFIERTFSSDDEIKNR-AKEFKLRWDQKY-MCIAGKLDR 313 Query: 291 RDVKWNSSENDWESITFHLSLILRSIFEKYSLRPLITLKNNRLTVI--ALDIRSKLPGKL 348 D + +EN S + + L + P +K N ++ A D S++ + Sbjct: 314 NDESISFTENQLASDS--VFEFLEGELSAFPFPPHFFMKGNVGIILIEATDSWSEMHASV 371 Query: 349 RLQQALDALQHIHSDAKLKDLQLV-IGVSKSHRGLKHAYAAYQEAVQALSLYSCYQKS-- 405 + L+ + + + V G+S + L A+ EA AL + + Sbjct: 372 -----ISFLEQFQTQVSAQFKRTVSFGISNICQKLIDVPDAFTEASDALQSGHLSRSTAF 426 Query: 406 ILFYEELGVFQLL--LSLNDGKTLENFIRSYLGPLIDHDSAKGSELLLTLRVYLDHDGSK 463 I Y V +LL L + D L+ F S L L + S LL TL VYL+ Sbjct: 427 IQVYHAKDVPELLRLLPVED---LKKFYNSTLQSLAEKQQEDQS-LLHTLSVYLETHCQI 482 Query: 464 QIAARNLFIVRQSLYYRLDKITELLGEDFMLPENRISIQVALRAYQLL 511 A+ L++ R ++ YRL+K ELLG+ PE + +++ALR +L+ Sbjct: 483 SETAKRLYVHRNTVIYRLEKCEELLGKSLKDPETTMRLRLALRMQRLI 530 >BH0757 [TQ] COG2508 Regulator of polyketide synthase expression Length = 557 Score = 64.7 bits (156), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 104/502 (20%), Positives = 217/502 (43%), Gaps = 45/502 (8%) Query: 31 PSSL-SFMQNLIDKNAACLCIELGDYFSSIPQEMIDLANRHDFPLIIFPRTVRFVDITLD 89 P++L ++++ ++ A L ++ + +IP +++ ANR FP+I P + + Sbjct: 65 PNALIELVRHMAEQGCAGLGLKTKRFIDTIPDDVLAEANRLGFPIIELPLRYSLGAMLNE 124 Query: 90 LHSLIINRHHRMLQELESISREFHRLTLTSQGTIKVLQLLCKSTRTQIVYMQPQGKPLFF 149 I+ L ++ REF + L+ +G ++++ L ++ + + Sbjct: 125 SLGFILKERTNELNYALTLHREFTNMILSGKGFSQIIEKLAAILEAPVILLNER-----L 179 Query: 150 PALSPEDQAPLLSFFEAF--------GEEMEGVQPDAAPYIRDYG-HKTIALKPVGALDQ 200 ++ + FFE + E+ + Q P ++T L P+ + +Q Sbjct: 180 DIMAATHKMDKDGFFEVYWFIHEINHAEDTQTYQQCTLPQTNTTNPYRTFFLYPIHSANQ 239 Query: 201 TWAYILMVCNHKP--QEFDCLLLDSASLSIAQELLRTRYMEERKLFSENLWVDELIGGR- 257 +++++ N P + L L+ A+ I+ EL++ +E+ +N + EL+ Sbjct: 240 QKGFLVVLGNLSPDAKGLQILALEQAANVISFELMKMLAIEQHARQLKNDFFCELVESLI 299 Query: 258 TQDDNRLKGLVGPDFNVVNELPYRVCLVEIENSRDVKWNSSENDWESITFHLSL------ 311 T+++ L G + + L Y VCL S+ + +S + + + + L Sbjct: 300 TREEEILNR--GKAYQLDKSLQY-VCLA----SKFDEMSSFQTEVQPLYMEKELRQQREQ 352 Query: 312 ---ILRSIFEKYSLRPLITLKNNRLTVIALDIRSKLPGKLRLQQALDALQHIHSD-AKLK 367 +L S +K+ ++ K + ++ I + +DA++ I + ++ Sbjct: 353 MFELLNSYLKKHFDHSILFTKGDLFVIL---IGFSYYNDAVEKTFIDAIRDIQQELNQML 409 Query: 368 DLQLVIGVSKSHRGLKHAYAAYQEAVQALSLYSCYQKS----ILFYEELGVFQLLLSLND 423 D GVS + A+QEAV AL S Y+++ I Y + +L S+ Sbjct: 410 DFSFSFGVSNYTERVTDIATAFQEAVDALR--SGYRENKKRFIKTYRIKEMTELFKSIPQ 467 Query: 424 GKTLENFIRSYLGPLIDHDSAKGSELLLTLRVYLDHDGSKQIAARNLFIVRQSLYYRLDK 483 K L+ F +S L L ++ + +LL TL +L+H A+++FI R ++ YR+ K Sbjct: 468 QK-LKEFYQSSLKDLAFPETKEKQDLLETLDSFLNHHCQISETAKSMFIHRNTVIYRIKK 526 Query: 484 ITELLGEDFMLPENRISIQVAL 505 E+LG D + +++AL Sbjct: 527 CEEILGIDLKGGHETLQLRMAL 548 >CAC1426 [TQ] COG2508 Regulator of polyketide synthase expression Length = 397 Score = 63.2 bits (152), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 38/136 (27%), Positives = 73/136 (53%), Gaps = 2/136 (1%) Query: 353 ALDALQHIHSDAKLKDLQLVIGVSKSH-RGLKHAYAAYQEAVQALSLYSCYQKSILFYEE 411 A++ ++ I S AK + + +G S R K + + ++ L L + +++ Y+E Sbjct: 233 AMNIIEEIISKAKNTSVNIGVGGEFSELRDFKISVSEAHRCLKVLKLNND-NNTVISYDE 291 Query: 412 LGVFQLLLSLNDGKTLENFIRSYLGPLIDHDSAKGSELLLTLRVYLDHDGSKQIAARNLF 471 LG+++L +++ + ++ F L LID+DS +EL+ TL +Y+ + A LF Sbjct: 292 LGIYKLFFQMDNLEEMKKFYLDVLKKLIDYDSKNTTELMNTLEMYIKESTNINRTAEKLF 351 Query: 472 IVRQSLYYRLDKITEL 487 I R +L YR+ +I E+ Sbjct: 352 IHRNTLKYRIRRIEEI 367 Score = 61.6 bits (148), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 25/87 (28%), Positives = 53/87 (60%) Query: 1 VHVLESAGFESLIHGEEMILTTGMGASAGLPSSLSFMQNLIDKNAACLCIELGDYFSSIP 60 V V+E + + G E+IL +G+ + S ++F++++ ++ + + I +G Y IP Sbjct: 33 VQVVEYPEYSKWLKGGELILISGIAIKNNINSLVNFIRDINSRSLSGIVISIGPYIKEIP 92 Query: 61 QEMIDLANRHDFPLIIFPRTVRFVDIT 87 +E+I++ N+ DFP+ P V+F+D++ Sbjct: 93 KEVIEIGNQLDFPIFEIPFEVKFIDVS 119 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,770,815 Number of extensions: 1495555 Number of successful extensions: 5365 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 5361 Number of HSP's successfully gapped: 10 Length of query: 526 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 415 Effective length of database: 25,576,357 Effective search space: 10614188155 Effective search space used: 10614188155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)