BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0007.p (280 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1631_2 [IR] COG3240 Phospholipase/lecithinase/hemolysin 119 4e-27 DR0758 [S] COG1801 Uncharacterized conserved protein 110 4e-24 TVN1119 [S] COG1801 Uncharacterized conserved protein 97 2e-20 Ta0990 [S] COG1801 Uncharacterized conserved protein 96 4e-20 Ta0973 [S] COG1801 Uncharacterized conserved protein 89 8e-18 >STM1631_2 [IR] COG3240 Phospholipase/lecithinase/hemolysin Length = 267 Score = 119 bits (299), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 12/273 (4%), Positives = 47/273 (17%), Gaps = 23/273 (8%) Query: 1 MIKIGLTGFGDHEELYG-----KIKPAERLPVYSA--HFPIVEIDSSFYAVQPVRNYAKW 53 + ++ G + G Y + + Sbjct: 1 ITRLVFFGDS-LSDSLGRMFEKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLN 59 Query: 54 AEQTPEGFQFIVKAYQGMTGHLRSKKNYFDTPEEMYQAFHASIEPVRSAGKLAMVLFQFP 113 + + + + +I + + + + Sbjct: 60 FAEGGSTSASYSCF-NCIGDFVSNTDR--QVASYTPSHQDLAIFLLGANDYMTLHKDNVI 116 Query: 114 PWFDCTKDNVEFLREAKERIQDVPSAVEFRNESWYSPEMRGKTLHFLEQEGWIHTVADEP 173 + D++E + + + + + + + + Sbjct: 117 MVVEQQIDDIEKIISGGVNN-VLVMGIPDLSLTP----YGKHSDEKRKLKDESIAHNALL 171 Query: 174 QAGSGSIPIVPVSTGTDATYVRLHGRNISGWNQSSHPDWRKLRYLYRYSTEELTEWRDRL 233 + + Y +++ Y Sbjct: 172 KT-NVEELKEKYPQ-HKICYYETADAF-KVIMEAASNI----GYDTENPYTHHGYVHVPG 224 Query: 234 LELEKTCRELYVVFNNNSGGDATDNAKELQSLL 266 + + VFN+ + L Sbjct: 225 AKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLE 257 >DR0758 [S] COG1801 Uncharacterized conserved protein Length = 275 Score = 110 bits (274), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 91/277 (32%), Positives = 128/277 (46%), Gaps = 27/277 (9%) Query: 2 IKIGLTGFGDHE-----ELYGKIKPAERLPVYSAHFPIVEIDSSFYAVQPVRNYAKWAEQ 56 + IG G+ D + +Y +K L Y+ HF VE++SSFYA+ ++ + + Sbjct: 3 VYIGCGGYTDEDWTAPGLIYEGVKKDAFLETYAHHFDAVELNSSFYAIPGLKAFEGMVRK 62 Query: 57 TPEGFQFIVKAYQGMTGHLRSKKNY-FDTPEEMYQAFHASIEPVRSAGKLAMVLFQFPPW 115 + +F VK + T H+R+ + FD M Q S EP+R AG + L QFP Sbjct: 63 SGGWVRFAVKLNKVFT-HVRAPTDTDFD---RMLQ----SPEPLREAGMMGPYLAQFPYS 114 Query: 116 FDCTKDNVEFLREAKERIQDVPSAVEFRNESWYSPEMRGKTLHFLEQEGWIHTVADEPQA 175 F T N ++L ER AVE R+ SW PE+R F G I D P Sbjct: 115 FHRTAANRKYLALLTERFAGHELAVELRHASWDKPEVREGMGEF----GIIWVSPDYPPV 170 Query: 176 GSGSIPIVPVSTGTDATYVRLHGRNISGW--NQSSHPDWRKLRYLYRYSTEELTEWRDRL 233 G P V V+ D Y+RLHGRN W QS+ R+ Y Y+ E+ EW +++ Sbjct: 171 GGMPEPQVHVT--GDVGYLRLHGRNRETWWEGQSAGE-----RHDYLYNRAEMDEWAEKI 223 Query: 234 LELEKTCRELYVVFNNNSGGDATDNAKELQSLLGIDG 270 + ELYV F N +GG A N L+ L G Sbjct: 224 AAVAGDLSELYVFFQNTTGGHALKNIPMLREALNARG 260 >TVN1119 [S] COG1801 Uncharacterized conserved protein Length = 257 Score = 97.4 bits (241), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 86/279 (30%), Positives = 132/279 (47%), Gaps = 33/279 (11%) Query: 1 MIKIGLTGFGDHEELYGKIKPA---ERLPVYSAHFPIVEIDSSFYAVQPVRNYAKWAEQT 57 MI +G +G+ +EE G P +L YS F VE++S+FY +W E Sbjct: 1 MIFVGCSGWS-YEEWNGVFYPKSKINKLKFYSTMFNTVEVNSTFYREINNDIINRWIETV 59 Query: 58 P-EGFQFIVKAYQGMTGHLRSKKNYFDTPEEMYQAF-HASIEPVRSAGKLAMVLFQFPPW 115 + F F +K Q +T +K+ + +Y+ F ++ P+R+A L VL Q PP Sbjct: 60 RGKNFIFSIKVPQNITHKAILEKD--SNSKSLYETFIDKTVRPIRNARMLGAVLVQLPPS 117 Query: 116 FDCTKDNVEFLREAKERI---QDVPSAVEFRNESWYSPEMRGKTLHFLEQEGWIHTVADE 172 F N E + I + +AVE RN+ YS E K L E+ G D Sbjct: 118 F-----NSNLQTELLDTICCENEYRTAVEVRNKDLYSNEELRKEL---EKRGIAIVDVDS 169 Query: 173 PQAGSGSIPIVPVSTGTDATYVRLHGRNISGWNQSSHPDWRKLRYLYRYSTEELTEWRDR 232 + P+ +++G +YVRLHGR GW +S +P Y YRYS +E+++ + Sbjct: 170 TEH-----PLSNLNSGLKWSYVRLHGRAQLGW-KSQNP------YDYRYSEQEISDLSSK 217 Query: 233 LLELEKTCRELYVVFNNNSGGDATDNAKELQSLLGIDGG 271 + + K E++V FNN+ G A NA ++ S LGI Sbjct: 218 I--MAKNYDEIFVYFNNHPRGSAPINALKMASCLGISNN 254 >Ta0990 [S] COG1801 Uncharacterized conserved protein Length = 240 Score = 96.3 bits (238), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 88/273 (32%), Positives = 134/273 (49%), Gaps = 46/273 (16%) Query: 2 IKIGLTGFGDHEELYGKIKPA-----ERLPVYSAHFPIVEIDSSFYAVQPVRNYAKWAEQ 56 I+IG +G+ + GK P+ E Y+ F VEI+S+FY+ W + Sbjct: 3 IRIGCSGWY-YPHWAGKFYPSDLNKSEWFRYYAQKFDTVEINSTFYSFPNAARVRSWVKG 61 Query: 57 TPEGFQFIVKAYQGMTGHLRSKKNYFDTPEEMYQAFHASIEPVRSAGK-LAMVLFQFPPW 115 +PE F F VK + +T H++ K D EE+ + E + S GK L VLFQFPP Sbjct: 62 SPENFVFSVKVNRTIT-HVKRLK---DCDEEIGLFY----EIIGSLGKMLGCVLFQFPPS 113 Query: 116 FDCTKDNVEFLREAKERIQDVPSAVEFRNESWYSPEMRGKTLHFLEQEGWIHTVADEPQA 175 F + DN+ + +A + Q + VEFRN SW+S + R LE+ +H VA + Sbjct: 114 FKYSHDNLIMIEKALD--QRFCNVVEFRNISWFSEDARCD----LEKSD-LHVVAVSSER 166 Query: 176 GSGSIPIVPVSTGTDA-TYVRLHGRNISGWNQSSHPDWRKLRYLYRYSTEELTEWRDRLL 234 +P+ D+ YVRLHG N++G Y Y+ E LT+ R++ Sbjct: 167 -------IPLWIKEDSVVYVRLHG-NVNG-------------YATDYNEEYLTDLAQRII 205 Query: 235 ELEKTCRELYVVFNNNSGGDATDNAKELQSLLG 267 +++ + LYV FNN+ G A NA L+S++ Sbjct: 206 DVKP--QSLYVYFNNDYSGYAPKNAIMLKSIIN 236 >Ta0973 [S] COG1801 Uncharacterized conserved protein Length = 257 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 79/272 (29%), Positives = 122/272 (44%), Gaps = 25/272 (9%) Query: 1 MIKIGLTG--FGDHEELYGKIKPAERLPVYSAHFPIVEIDSSFYAVQPVRNYAKWAE-QT 57 MI +G +G + + E+++ A+RL YS F VE+DS+FY + P +W + + Sbjct: 1 MIHLGCSGWSYDEWEKVFYPEHTADRLSFYSMIFNTVEVDSTFYRIPPKNVARQWGKAMS 60 Query: 58 PEGFQFIVKAYQGMTGHLRSKKNYFDTPEEMYQAFHASI-EPVRSAGKLAMVLFQFPPWF 116 + F F VK +T D +++ F ++ + ++G L +LFQ PP F Sbjct: 61 GKHFLFAVKVPGDITHDAIFTD--MDRSCSIFRTFEQNVLNELSTSGVLGPILFQMPPRF 118 Query: 117 DCTKDNVEFLREAKERIQDVPSAVEFRNESWYSPEMRGKTLHFLEQEGWIHTVADEPQAG 176 DNVE L E + +AVE RN + Y K +E G D + Sbjct: 119 SV--DNVERLISFMECVHVRNAAVEVRNMTLYENR---KFREAIEGLGVAMVDVDSTERR 173 Query: 177 SGSIPIVPVSTGTDATYVRLHGRNISGWNQSSHPDWRKLRYLYRYSTEELTEWRDRLLEL 236 I +G YVRLHGRN GW + D YRY+ EL E ++ + Sbjct: 174 LNRI-----ESGLKWAYVRLHGRNPGGWKAENPFD-------YRYTDAELREIASKIKSV 221 Query: 237 EKTCRELYVVFNNNSGGDATDNAKELQSLLGI 268 +++V FNN+ G A NA L ++G+ Sbjct: 222 NYD--DIFVYFNNHPHGSAPVNAMALSRMVGV 251 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,857,570 Number of extensions: 920831 Number of successful extensions: 3196 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3175 Number of HSP's successfully gapped: 17 Length of query: 280 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 175 Effective length of database: 26,268,361 Effective search space: 4596963175 Effective search space used: 4596963175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)