BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0017.p (348 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0008 [T] COG2206 HD-GYP domain 170 3e-42 BH3794 [T] COG2206 HD-GYP domain 122 8e-28 CAC0277 [T] COG2206 HD-GYP domain 112 1e-24 CAC2620 [T] COG2206 HD-GYP domain 109 6e-24 VC2497 [T] COG2206 HD-GYP domain 94 2e-19 >BH0008 [T] COG2206 HD-GYP domain Length = 360 Score = 170 bits (431), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 110/359 (30%), Positives = 192/359 (53%), Gaps = 11/359 (3%) Query: 1 VRIHIKKLKIGDVLKTDIFNRQGLHVLAKKTKIRLEDIALLNKHSIKFVDVEGSV----- 55 +++ + +L G ++ I + +KT + EDI L+K I ++VE + Sbjct: 1 MKVKLNQLVPGCIVLKPIMAETTTPLYKEKTVLTAEDIQTLSKFLIDEIEVENVLANGEK 60 Query: 56 ----EDRIAREVFNGTERPLRVKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLE 111 ++ + E E R K Y +++ Y+ F E ++ + L+PL E Sbjct: 61 FIPSDNGLGAEAAALMETGDRFKDRYMQAVANYKKVFQEWAAGTPVEMECVRHILLPLFE 120 Query: 112 QLDEQKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIG 171 ++ E+KDVL L + +++ +Y+H++ VGLLS ++ LGY K E +I AG L D G Sbjct: 121 EIVEKKDVLLQLHHYSTKEDYLYHHAVSVGLLSAFLGAKLGYEKGEWLKIALAGALADCG 180 Query: 172 KSRVRLSIRNRVGHLNDEEEKELQKHTSAGYQIIRNSLKDDTAALVA-LQHHEREDGTGY 230 +++ S+ + G +E+ E++ H YQ+++ + D + L A LQHHEREDGTGY Sbjct: 181 MAKLPTSLLTKNGLYTLKEKHEMRDHPKYSYQMLKKRPEIDGSLLSAVLQHHEREDGTGY 240 Query: 231 PLGVHKEKIHPYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQAL 290 PLGV +KIH YAQI+AVA+ + + + K++ VL+++ + FGK N + V L Sbjct: 241 PLGVRGQKIHFYAQILAVADVYHAMISERKYRKKKSPYMVLEDLAKERFGKFNPRIVHVL 300 Query: 291 IHNLLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKET-FIDLSKERGLDIREIIV 348 + L + V+L+N +GEI+ +PM RP+V+++ I L K + I ++++ Sbjct: 301 VSQLTQLSVRTSVRLTNNRLGEIVFIDPMAPTRPMVQLQTGEMIYLMKNYTVHIEDVLL 359 >BH3794 [T] COG2206 HD-GYP domain Length = 231 Score = 122 bits (306), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 78/223 (34%), Positives = 120/223 (53%), Gaps = 2/223 (0%) Query: 126 FQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRVRLSIRNRVGH 185 + +D+ Y H++ V +LS I WL + E+ LHDIGK + +I N+ G Sbjct: 4 IKAKDDYTYRHTVGVSILSRLIGTWLKLDEVILKELTLGAVLHDIGKVEIPDAILNKPGA 63 Query: 186 LNDEEEKELQKHTSAGYQIIRNS-LKDDTAALVALQHHEREDGTGYPLGVHKEKIHPYAQ 244 L +E + +Q HT GY+II+ S + DT AL+AL+HHEREDGTGYP + +IH ++ Sbjct: 64 LTKDEYRIIQDHTVKGYRIIQESGIYSDTVALMALEHHEREDGTGYPYQKTRNEIHLLSK 123 Query: 245 IVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQALIHNLLPNMIGKKVQ 304 I+AVA+ F + T + VL +M + FGKL + + + IG K + Sbjct: 124 ILAVADVFHAMTSDRIYRKGMALYDVLLQMRQDRFGKLEPRITDFFVRRFMEQCIGSKAR 183 Query: 305 LSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIREII 347 L++G EI+ + P+VK +IDL + L I+E+I Sbjct: 184 LNDGSRVEIVFIPYENPICPIVKQGTNYIDL-RMSPLYIQELI 225 >CAC0277 [T] COG2206 HD-GYP domain Length = 372 Score = 112 bits (279), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 95/335 (28%), Positives = 163/335 (48%), Gaps = 13/335 (3%) Query: 4 HIKKLKIGDVLKTDIFNRQGLHVLAKK---TKIRLEDIALLNKHSIKFVD--VEGSVEDR 58 I+ +K G +L +++ +G +L + T+ ++ I +N ++I D E +ED Sbjct: 5 QIENVKEGSILGKTLYDGEGRVLLTRGSVLTQAIIDKIKSINIYTIYIEDEYSEAIIEDV 64 Query: 59 IAREVFNGTERPLR---VKPNYDKSLREYQAFFLEAIISGEIDRSKIEWTLMPLLEQLDE 115 I E+ + +R + N + Y L E D I + ++E++ Sbjct: 65 IKPEIRRTAIKMVRNTFYRLNKYNNKENYTGRNLYK--ESEQDFKSIYYIATEIIEEILS 122 Query: 116 QKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRV 175 QK+V+ L + D+ Y HS+ V ++S I L + K E F++C LHD+GK + Sbjct: 123 QKNVMIGLVDIKSFDDYTYQHSVNVAVISLIIGIKLKFHKYELFDLCIGALLHDVGKMFI 182 Query: 176 RLSIRNRVGHLNDEEEKELQKHTSAGYQIIRNSLKDDTAA-LVALQHHEREDGTGYPLGV 234 I N+ G L +EE K +++HT GY ++ + A +++LQHHER G GYP G Sbjct: 183 PSEILNKKGELTEEEFKIMKEHTVRGYDYLKGLYEISVPARIISLQHHERAGGQGYPEGR 242 Query: 235 HKEKIHPYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQALIHNL 294 E+I ++IVAVA+ + + T + + L+ + K + K VQA ++ Sbjct: 243 SGERISILSRIVAVADVYDAMTSDRVYRRAIAPNEALEYIMANSGSKFDYKVVQAFSKSV 302 Query: 295 LPNMIGKKVQLSNGHIGEIILNNPMDF-FRPLVKV 328 +P G V+LSN IG ++ DF RP +K+ Sbjct: 303 IPYPEGSIVRLSNNEIG-VVTKIHEDFPLRPEIKI 336 >CAC2620 [T] COG2206 HD-GYP domain Length = 345 Score = 109 bits (273), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 70/225 (31%), Positives = 118/225 (52%), Gaps = 7/225 (3%) Query: 126 FQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLHDIGKSRVRLSIRNRVGH 185 F IY HS+ V +S+ + +WLG + E + A LHD GK+++ SI + Sbjct: 126 FYGSKNNIYRHSVNVAAISFILGKWLGMNEEEINLLTYASLLHDFGKTQLDNSILKKESS 185 Query: 186 LNDEEEKELQKHTSAGYQIIRNSLKDDTAA--LVALQHHEREDGTGYPLGVHKEKIHPYA 243 L EE + H Y +I+ + D A+ L L HHE+ DG+GYPLG+ KIH +A Sbjct: 186 LTPEEYAVYKTHPVIAYHLIK-EIPDINASVGLGVLMHHEQIDGSGYPLGIKDNKIHKFA 244 Query: 244 QIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPVQALIHNLLPNMIGKKV 303 +I+A+A+ F V+++ F L+ + +L GKL+ I++ + +G+ V Sbjct: 245 KIIAIADIFDK---VNSDNTNGPFEG-LKAIKDLSLGKLDCSYSNMFINHAVNYYMGESV 300 Query: 304 QLSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIREIIV 348 LS+ +II + + +PL+ + F+DL E+ L ++ +IV Sbjct: 301 TLSDNRSCKIIKLDTENLTKPLLLADDGFLDLKSEKDLYVKSLIV 345 >VC2497 [T] COG2206 HD-GYP domain Length = 417 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 71/238 (29%), Positives = 114/238 (47%), Gaps = 1/238 (0%) Query: 109 LLEQLDEQKDVLTLLFSFQKEDETIYNHSLQVGLLSYYIAEWLGYPKSERFEICRAGYLH 168 ++++L +V L + + E E IY HSL V +++ I + G P E+ A H Sbjct: 144 IVDKLLSDDNVTLHLMNSKSEFEDIYFHSLNVSVIAMMIGKARGLPAERIKELAFAALFH 203 Query: 169 DIGKSRVRLSIRNRVGHLNDEEEKELQKHTSAGYQIIRN-SLKDDTAALVALQHHEREDG 227 DIGK RV +I + L + EE L+ HT G ++ N ++A V QHHE DG Sbjct: 204 DIGKVRVPTAIVRKKTPLTEPEENYLRLHTKYGIELADNIETFPESAKRVIEQHHELRDG 263 Query: 228 TGYPLGVHKEKIHPYAQIVAVANEFISKTFVSAEQPKRNFSTVLQEMYELGFGKLNEKPV 287 +GYPLG+ + I AQIVAVAN F + + ++ L +++ N + + Sbjct: 264 SGYPLGLKGDDIDELAQIVAVANAFDNLCHPNIPSEQKIPYVALSHLFKNCKHIYNAENL 323 Query: 288 QALIHNLLPNMIGKKVQLSNGHIGEIILNNPMDFFRPLVKVKETFIDLSKERGLDIRE 345 L+ + G VQLSN +G +I N P V + + + S+ +D+ + Sbjct: 324 GILVKFMGVFPPGTVVQLSNDMVGLVISVNASSLLYPNVLIYDPSVPRSQAPIIDLAD 381 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,111,607 Number of extensions: 1062903 Number of successful extensions: 3613 Number of sequences better than 1.0e-05: 37 Number of HSP's gapped: 3578 Number of HSP's successfully gapped: 37 Length of query: 348 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 241 Effective length of database: 26,037,693 Effective search space: 6275084013 Effective search space used: 6275084013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)