BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0175.0016.p (402 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEI0181 [GEPR] COG0477 Permeases of the major facilitator super... 237 2e-62 CC0403 [GEPR] COG0477 Permeases of the major facilitator superfa... 234 2e-61 all0371 [GEPR] COG0477 Permeases of the major facilitator superf... 198 1e-50 XF0993 [GEPR] COG0477 Permeases of the major facilitator superfa... 195 1e-49 Cgl0123 [GEPR] COG0477 Permeases of the major facilitator superf... 64 3e-10 >BMEI0181 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 412 Score = 237 bits (605), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 152/393 (38%), Positives = 216/393 (54%), Gaps = 2/393 (0%) Query: 3 HNRSFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXXX 62 + +F + W AR S A Q++SV++GWQ+Y LT AF LG+VGL QF Sbjct: 16 RHSAFKKYWGARFLSAFAVQIVSVSVGWQIYDLTRDAFNLGMVGLVQFLPALLLVLVTGA 75 Query: 63 XXXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASAA 122 L ++E ++ G R F GP+SA+ Sbjct: 76 AADRFGRRLIMG-LSLILEGIVTAALLAFTLAGLFEPLTVFAALLIFGVARAFLGPSSAS 134 Query: 123 LVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIASLGALLVSTVLIF 182 LV ++V E Q A IVGP GG+L AAY + L+ ++LIF Sbjct: 135 LVVNLVPTEDFANAVSWNSSAWQVATIVGPVAGGLLYGISPVAAYSIATLFLIAGSLLIF 194 Query: 183 FVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAEDIL 242 + ++ ++ T L+G R+++ KI+LG ISLDLFAVLLGG ALLPI+A DIL Sbjct: 195 SIPKPRQHTLIEERSISTILAGFRYIWKEKIVLGAISLDLFAVLLGGTVALLPIYARDIL 254 Query: 243 KTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHSFWL 302 G GLGLLRSAP +GA++ +L L + + G+ +F + +F ++FG+S WL Sbjct: 255 DLGPWGLGLLRSAPGIGAVLTALWLAGHPIRDHAGRAMFLFVGLFGFFNVIFGLSTLTWL 314 Query: 303 SLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTMAGL 362 S+ AL L GA+D++SV IR TL+Q+ TP M+GRVNAVN +F+G SN+LGEF +G MA Sbjct: 315 SILALALAGAADMISVYIRETLMQLWTPDHMRGRVNAVNMVFVGASNELGEFRAGVMAAA 374 Query: 363 VGAVPATVIGGLGTLVISFIWMYWLFPVLRTQK 395 +GAVPA VIGG+ + ++ +FP LR + Sbjct: 375 IGAVPAVVIGGIRAIAVAAG-WAAMFPQLRKAR 406 >CC0403 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 429 Score = 234 bits (597), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 159/402 (39%), Positives = 221/402 (54%), Gaps = 15/402 (3%) Query: 1 VLHNRSFLRLWIARIFSTSAFQMLSVAIGWQMYALT-------DSAFQLGLVGLAQFXXX 53 +L R FL W AR ST Q+ SVA+GWQ+YA+ +SAF + ++GLAQF Sbjct: 27 LLRERDFLLFWAARFVSTLGVQIQSVALGWQVYAIARMTKSVGESAFLVSMIGLAQFLPL 86 Query: 54 ---XXXXXXXXXXXXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXX 110 L + GS Q W Sbjct: 87 FLLTLVAGETADRRTRKLIVAATLALDAISAGVLLVLALMGSHQLWPIFAISVLFGA--- 143 Query: 111 XCRTFEGPASAALVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLVVWGTAAAYIAS 170 R F PAS+A+ P +V R LP+ Q I GP+LGG+L++ A A+ AS Sbjct: 144 -SRAFLSPASSAMGPMLVPRPLLPRAIAWNSLSWQAGSIGGPALGGLLLIHSPALAFGAS 202 Query: 171 LGALLVSTVLIFFVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGA 230 G LV+ +L ++ + ++ GL +V+ KI+ G+ISLDLFAV+LGGA Sbjct: 203 FGLYLVAALLALSIRKPTKPEPQPGSRVELIKEGLSYVWNNKIVFGSISLDLFAVILGGA 262 Query: 231 TALLPIFAEDILKTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALA 290 TALLP+FA+D+L G G GLLR+APA+GA +V L L + R GK +FA + VF LA Sbjct: 263 TALLPVFAKDVLHIGPGGFGLLRAAPAIGACLVGLYLAANPIRRHAGKIMFAGVAVFGLA 322 Query: 291 TILFGVSHSFWLSLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQ 350 T++FG+S WLS+ AL ++G +D++SV +R TLVQ+ TP M+GRV AV+ +FI SN+ Sbjct: 323 TVVFGLSKLVWLSVAALAVLGGADMLSVYVRQTLVQIVTPDAMRGRVAAVSGVFISASNE 382 Query: 351 LGEFESGTMAGLVGAVPATVIGGLGTLVISFIWMYWLFPVLR 392 LGE ESG A L+G + A V GG+G + ++ IW + LFP LR Sbjct: 383 LGEVESGAAAWLLGPIGAAVFGGVGAMAVTAIWAF-LFPELR 423 >all0371 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 422 Score = 198 bits (504), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 132/380 (34%), Positives = 200/380 (52%), Gaps = 4/380 (1%) Query: 2 LHNRSFLRLWIARIFSTSAFQMLSVAIGWQMYALTDSAFQLGLVGLAQFXXXXXXXXXXX 61 L R + + R+ + QM +VAIGW++Y T+SA LG VGLAQ Sbjct: 24 LRFRDYRLFTLGRVLLSVGSQMQTVAIGWELYERTNSAMVLGGVGLAQVLPMIALTLVAG 83 Query: 62 XXXXXXXXXXXXXXLCQLMESXXXXXXXXGSMQGWXXXXXXXXXXXXXXXCRTFEGPASA 121 L+ +G R F PAS Sbjct: 84 DIADRRSRKLTILLSVMLLALCSLGLAVLSYTRG--AVFLIYGCLALIGVGRAFLKPASD 141 Query: 122 ALVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGV-LVVWGTAAA-YIASLGALLVSTV 179 A++ ++ Q A + GP+LGG+ + V G+A YI + A + + Sbjct: 142 AIMWQLIPVNVFTNAATWNSSSFQLASVAGPALGGLGIAVLGSATGVYILAAIAGFLCFL 201 Query: 180 LIFFVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPIFAE 239 + +K + + +++ +G +F++ ++IL I+LD+FAVLLGGA ALLP+FA+ Sbjct: 202 FMAAIKEKTVERVTEPISLQVLAAGAKFIWENQLILAAITLDMFAVLLGGAVALLPVFAK 261 Query: 240 DILKTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALATILFGVSHS 299 DIL+ G + LG L++AP++GA+++++ L R G L S++ F + TI+FG+S Sbjct: 262 DILQVGPVELGYLQAAPSIGALIMAVTLAYLPPLRKAGTALLWSVIGFGVVTIIFGLSRW 321 Query: 300 FWLSLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQLGEFESGTM 359 FWLSL L L GA D +SVVIR TLVQ+ TP +++GRV A+NS+FI SN+LG FESG Sbjct: 322 FWLSLIMLTLSGALDTISVVIRHTLVQIRTPDQLRGRVAAINSVFISASNELGGFESGLT 381 Query: 360 AGLVGAVPATVIGGLGTLVI 379 A L G V + V GG+GT+++ Sbjct: 382 AALFGPVLSVVGGGIGTILV 401 >XF0993 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 108/269 (40%), Positives = 170/269 (63%), Gaps = 2/269 (0%) Query: 113 RTFEGPASAALVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLV-VWGTAAAYIASL 171 R+F P AL ++ RE + Q ++++ P LGG+L+ + G AY A + Sbjct: 131 RSFIVPVYNALFARVLAREDYARGAGLGTVVFQASLVLSPMLGGILIALGGKGLAYAAGV 190 Query: 172 GALLVSTVLIFFVKTVH-FVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGA 230 A +++ + + ++ V K A + + GLRFVF+ +I+LG ++LD+F+VLLGGA Sbjct: 191 VASIIALLALALLRVEEPAVDKPRAPVLGSIAEGLRFVFSNQIMLGAMALDMFSVLLGGA 250 Query: 231 TALLPIFAEDILKTGSLGLGLLRSAPAVGAIVVSLILTRYSVERAIGKTLFASLVVFALA 290 ++LP F +DI G GLG+LR PA+G+++ L L RY ++R G+TL ++ F + Sbjct: 251 VSMLPAFIQDIFHYGPEGLGILRGTPALGSVLAGLWLARYPLQRHAGRTLLLAVAGFGIC 310 Query: 291 TILFGVSHSFWLSLFALFLIGASDVVSVVIRSTLVQVNTPQEMQGRVNAVNSLFIGTSNQ 350 TI FG+S FW+S L + GA D +SVV+R++++Q+ TP EM+GRV+++NS+FIG+SN+ Sbjct: 311 TIAFGLSQHFWVSAGILLVYGACDGISVVLRASILQLVTPDEMRGRVSSINSIFIGSSNE 370 Query: 351 LGEFESGTMAGLVGAVPATVIGGLGTLVI 379 LG F G MA +G V A V+GG TLV+ Sbjct: 371 LGAFYDGVMARFIGLVHAVVLGGFMTLVV 399 >Cgl0123 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 424 Score = 64.3 bits (155), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 68/283 (24%), Positives = 134/283 (47%), Gaps = 19/283 (6%) Query: 118 PASAALVPDIVDREQLPKXXXXXXXXMQTAMIVGPSLGGVLV-VWGTAAAYIASLGALLV 176 P A++ I+ +QL MQT IVGP + G L+ + G Y + +++ Sbjct: 128 PTRTAILRSILPIDQLASATSLNMLLMQTGAIVGPLIAGALIPLIGFGWLYFLDVVSIIP 187 Query: 177 STVLIFFVKTVHFVKKLDAVNMDTFLSGLRFVFARKIILGTISLDLFAVLLGGATALLPI 236 + ++ + ++ K+ + + GL+++ + ++L + LDL A++ G AL P Sbjct: 188 TLWAVWSLPSIKPSGKVMKAGFASVVDGLKYLAGQPVLLMVMVLDLIAMIFGMPRALYPE 247 Query: 237 FAEDILKTGSLG---LGLLRSAPAVGAI---VVSLILTRYSVERAIGKTLFASLVVFALA 290 AE G G L + S+ AVGA+ V+S + R S + G ++ ++ + A Sbjct: 248 IAEVNFGGGDAGATMLAFMYSSMAVGAVLGGVLSGWVARISRQ---GVAVYWCIIAWGAA 304 Query: 291 TILFGVS-------HSFWLSLFALFLI--GASDVVSVVIRSTLVQVNTPQEMQGRVNAVN 341 L GV+ + W +F + ++ G +D+ S +R+ ++Q + + +QGR+ V Sbjct: 305 VALGGVAIVVSPGAVTAWAWMFIIMMVIGGMADMFSSAVRNAILQQSAAEHVQGRIQGVW 364 Query: 342 SLFIGTSNQLGEFESGTMAGLVGAVPATVIGGLGTLVISFIWM 384 + + +L + G A +GA + GG+ +V++ I M Sbjct: 365 IIVVVGGPRLADVLHGWAAEPLGAGWTVLWGGVAVVVLTAICM 407 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,915,689 Number of extensions: 836835 Number of successful extensions: 3340 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 3323 Number of HSP's successfully gapped: 24 Length of query: 402 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 293 Effective length of database: 25,807,025 Effective search space: 7561458325 Effective search space used: 7561458325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)