BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0175.0006.p (369 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0183 [G] COG2706 3-carboxymuconate cyclase 195 1e-49 RSc0049 [G] COG2706 3-carboxymuconate cyclase 156 5e-38 RSp0820 [S] COG3391 Uncharacterized conserved protein 67 5e-11 RSc0127 [S] COG3391 Uncharacterized conserved protein 65 2e-10 MA1904_1 [S] COG3391 Uncharacterized conserved protein 63 7e-10 >RSp0183 [G] COG2706 3-carboxymuconate cyclase Length = 410 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 124/378 (32%), Positives = 190/378 (50%), Gaps = 47/378 (12%) Query: 2 KNTRLLLFTGSYASAEES----GVQVFEFNGEAGGTLTPLDQVQGITNPTFVNVDAAEGR 57 K L + GS + E + G+ VF + + GG L P+ V+ + NP+F+ ++ A R Sbjct: 54 KPMDLYAYVGSRTTRERNARGDGISVFRVDTQTGG-LAPVQLVKDLVNPSFLALNPAGDR 112 Query: 58 LYAIGEKANGQGGKEGEVVSFAINPQNGKLTELNRISTMPATGSGQTTTCHISRDTRNEF 117 LY + G ++ +FA++ +G+L+ +NR ST H++ D F Sbjct: 113 LYTVH-------GDLADISAFAVDRASGRLSFINRQSTQ------GKNPVHLALDPSGRF 159 Query: 118 IIVCSYHG-----------GLVG----LVSLDQKGLPVRLEDTAVHTGQRNLPGQEKPHP 162 ++V ++ G G +G LV LD P R+E Q++ P Sbjct: 160 VVVTNHIGASLAVLPLAADGALGEVTQLVKLDGPIGPHRIE-------------QKQAKP 206 Query: 163 HSAIFSPDEKFLFVSDLGLDLIRTYKINRDAKMLEALGDTTLHEGAGPRHFVFHPDGKSA 222 H F P +F+ V D GLD ++ + EGAGPRH F PDG+ A Sbjct: 207 HFNRFDPAGRFVIVPDKGLDRTFAFRFAEGRLTPATPSFVSAREGAGPRHVAFRPDGRVA 266 Query: 223 YVINELDSTVTSFLYDSAAGTLQTVETISTLPEGYPAANNSCAEIALSADGLYLYGSNRG 282 YV+NELDSTVT++ YD+ TL ++ + +LP+ + N+ +EI + A G ++Y SNRG Sbjct: 267 YVVNELDSTVTTYGYDAGTATLTPLQVLPSLPDTF-TGNSRASEIKVDAAGRFVYASNRG 325 Query: 283 HDSIVVYAVDPATARLTLVEHVSTHGGHPRHFALTPDGAYLIAANRDGNNLVVFSIDRAS 342 HDSI V+ +D A+ L V + G PR F + P G +L A N D +++V F+ID + Sbjct: 326 HDSIAVFRIDAASGLLRFVAAEPSLGRTPRFFTIAPGGRFLYALNEDSDSIVAFAIDPHT 385 Query: 343 GRLSFTGNTAALSKPVCV 360 GRL TG A PVC+ Sbjct: 386 GRLHPTGTRAQSGSPVCM 403 >RSc0049 [G] COG2706 3-carboxymuconate cyclase Length = 418 Score = 156 bits (395), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 115/359 (32%), Positives = 168/359 (46%), Gaps = 20/359 (5%) Query: 11 GSYASAEESGVQVFEFNGEAGGTLTPLDQVQGITNPTFVNVDAAEGRLYAIGEKANGQGG 70 G+ G+ V G+A QV NP+F+ V + LYAI E +G Sbjct: 67 GTAGGPPARGIYVLAPRGDAWSQA----QVVESANPSFLAVHPNQRCLYAINEIDVYEGR 122 Query: 71 KEGEVVSFAINPQNGKLTELNRISTMPATGSGQTTTCHISRDTRNEFIIVCSYHGGLVGL 130 G ++AI+P++G+LT LNR P + SG T H++ + + V Y GG + Sbjct: 123 ATGTAEAYAIDPRDGRLTLLNR---QPLSLSG-TIPAHVAVSPDGQHLAVALYGGGAYNV 178 Query: 131 VSLDQKGLPVRLEDTAVHTGQ-RNLPGQEKPHPHSAIFSPDEKFLFVSDLGLDLIRTYKI 189 + + G + TG + QE PH H +F P + + +DLG D I + + Sbjct: 179 LPIGADGRLGSVSGLFKDTGSGPDTARQEAPHAHMVLFDPTGRHILGTDLGTDRINVFAL 238 Query: 190 NRDAKMLEALGDTTLHEGAGPRHFVFHPDGKSAYVINELDSTVTSFLYDSAAG-TLQTVE 248 D K L L G GPRH HP GK YVI+ELDSTV YD A G L + Sbjct: 239 E-DGK-LTPRDRAQLKPGGGPRHLALHPSGKLLYVISELDSTVACHGYDPATGRVLDERQ 296 Query: 249 TISTLPEGYPAANNSCAEIALSADGLYLYGSNRG-------HDSIVVYAVDPATARLTLV 301 I+T P Y S A + + G +LY SNR DSI +++DPA+ RL + Sbjct: 297 RIATTPADY-TGQKSGAALVMHPSGRFLYASNRRLKSEHPLADSIAAFSIDPASGRLAAL 355 Query: 302 EHVSTHGGHPRHFALTPDGAYLIAANRDGNNLVVFSIDRASGRLSFTGNTAALSKPVCV 360 ++ S PR + DG +L A ++ G+ ++ ID A+GRL + + PVC+ Sbjct: 356 QYWSEGLRFPRALTMADDGRHLYALSQKGDTILRLRIDDATGRLDQPAVVSNVPTPVCL 414 >RSp0820 [S] COG3391 Uncharacterized conserved protein Length = 446 Score = 66.6 bits (161), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 73/294 (24%), Positives = 121/294 (41%), Gaps = 30/294 (10%) Query: 72 EGEVVSFAINPQNGKLTELNRISTMPATGSGQTTTCHISRDTRNEFIIVCSYHGGLVGLV 131 + V +FA++P G T + R + A G G + + D + V + V + Sbjct: 126 DNTVQAFALDPATGAATGVGRPA---AVGHGPVS---LVADPAGTTLYVVNASDNTVTTL 179 Query: 132 SLDQKGLPVRLEDTAVHTGQRNLPGQEKPHPHSAIFSPDEKFLFVSDLGLDLIRTYKINR 191 ++ V + AV TG P S +P F + ++ G + + +K++ Sbjct: 180 AIHPDTGAVSVSAPAVRTGAS---------PLSIARTPAGPFAYTANAGDNTLSIFKMSA 230 Query: 192 DAKMLEALGDTTLHEGAGPRHFVFHPDGKSAYVINEL----DSTVTSFLYDSAAGTLQTV 247 A+ G + G+ P + G AYV+N +V +F + A G L Sbjct: 231 KAEAPAPRG--VVQAGSNPYTVAVNGTGTFAYVVNAAMVSGAPSVMAFAINGATGAL--- 285 Query: 248 ETISTLPEGYPAANNSCAE-IALSADGLYLYGSNRGHDSIVVYAVDPATARLTLVEHVST 306 +P G PAA IAL G + Y +N D++ VYA++ AT L Sbjct: 286 -----VPAGSPAATGHAPFFIALHPAGRFAYVANFADDTLSVYAINGATGALAPAGSPVA 340 Query: 307 HGGHPRHFALTPDGAYLIAANRDGNNLVVFSIDRASGRLSFTGNTAALSKPVCV 360 GG+P A+ P G + AN + +F+ID +G L+ G A + PV + Sbjct: 341 TGGNPFAIAIHPSGRFAYVANVFSGTISLFAIDARTGALTPIGTVQAGASPVAM 394 >RSc0127 [S] COG3391 Uncharacterized conserved protein Length = 480 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 75/326 (23%), Positives = 131/326 (40%), Gaps = 35/326 (10%) Query: 41 QGITNPTFVNVDAAEGRLYAIGEKANGQGGKEGEVVSFAINPQNGKLTELNRISTMPATG 100 G N T + V + L+A +N + V ++ ++P G T I T + G Sbjct: 134 MGSANVTNIAVTCSTRPLFAYTANSN-----DNTVSAYTLDPTTGAPT---LIGTPISVG 185 Query: 101 SGQTTTCHISRDTRNEFIIVCSYHGGLVGLVSLDQKGLPVRLEDTAVHTGQRNLPGQEKP 160 G + D +F+ V + V +++D + + + T TG + Sbjct: 186 QGP---LSLIADKAGKFVYVVDGNDNSVTTLAIDSETGLLTISGTPATTGMQ-------- 234 Query: 161 HPHSAIFSPDEKFLFVSDLGLDLIRTYKINRDAKMLEALGDTTLHEGAGPRHFVFHPDGK 220 P + +P F + ++ G + + + IN +L ++G T+ G P V + G Sbjct: 235 -PFNIARTPASTFAYTTNFGDNTLSGFSINATTGVLTSIG--TIAAGTNPYTIVINKAGT 291 Query: 221 SAYVINELDSTVTS----FLYDSAAGTLQTVETISTLPEGYPAAN-NSCAEIALSADGLY 275 AYV+N T F + A G L V G P A N+ IAL G + Sbjct: 292 FAYVVNAASGAGTPSAMVFSINGATGALTAV--------GSPVATGNAPFYIALHPAGTF 343 Query: 276 LYGSNRGHDSIVVYAVDPATARLTLVEHVSTHGGHPRHFALTPDGAYLIAANRDGNNLVV 335 Y +N +++ VYA++ T LT V G P A+ P G + N N + + Sbjct: 344 AYVANSQDNTVQVYAINQTTGALTAVGSPVATGQGPIPIAIHPSGLFAYVGNVFDNTVSL 403 Query: 336 FSIDRASGRLSFTGNTAALSKPVCVK 361 ++I+ +G L+ A + P+ + Sbjct: 404 YAINTGTGALTLKSTLATGNDPLAIT 429 Score = 60.8 bits (146), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 47/148 (31%), Positives = 70/148 (47%), Gaps = 10/148 (6%) Query: 169 PDEKFLFVSDLGLDLIRTYKINRDAKMLEALGDTTLHEGAGPRHFVFHPDGKSAYVINEL 228 P F +V++ + ++ Y IN+ L A+G + + G GP HP G AYV N Sbjct: 339 PAGTFAYVANSQDNTVQVYAINQTTGALTAVG-SPVATGQGPIPIAIHPSGLFAYVGNVF 397 Query: 229 DSTVTSFLYDSAAGTLQTVETISTLPEGYPAANNSCAEIALSADGLYLYGSNRGHDSIVV 288 D+TV+ + ++ G L T++T + N IA +G+ HD +V Sbjct: 398 DNTVSLYAINTGTGALTLKSTLATGNDPLAITLNDAGSIAYIVNGV-------DHD-VVA 449 Query: 289 YAVDPATARLTLVEHVSTHGGHPRHFAL 316 YAVD +T LT V T G PR FA+ Sbjct: 450 YAVDSSTGALTSVATAQT-GLLPRAFAI 476 >MA1904_1 [S] COG3391 Uncharacterized conserved protein Length = 361 Score = 63.2 bits (152), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 58/209 (27%), Positives = 90/209 (43%), Gaps = 25/209 (11%) Query: 140 VRLEDTAVHTGQRNLPGQEKPHPHSAIFSPDEKFLFVSDLGLDLIRTYKINRDAKMLEAL 199 V + DTA + +P P SPD K ++V++L + I + + Sbjct: 122 VSVIDTATNAVTATVPAGS--SPQGIAVSPDGKTIYVANLAGNSISVIDTTSNTVV---- 175 Query: 200 GDTTLHEGAGPRHFVFHPDGKSAYVINELDSTVTSFLYDSAAGTLQTVETISTLPEGYPA 259 +T+ G P PDGK YV N D TV+ + D+A + + ++ P Sbjct: 176 --STVKTGRNPTGVAVSPDGKKVYVTNSEDKTVS--IIDTATKVVISTVSVGKDPR---- 227 Query: 260 ANNSCAEIALSADGLYLYGSNRGHDSIVVYAVDPATARLTLVEHVSTHGGHPRHFALTPD 319 EIA++ DG +Y +N DS V +D +T +T V G P A+TPD Sbjct: 228 ------EIAVTPDGAKVYVAN--SDSGTVSVIDVSTNSVTDTVKVE---GAPFGVAVTPD 276 Query: 320 GAYLIAANRDGNNLVVFSIDRASGRLSFT 348 GA + N D V ID A+ +++ T Sbjct: 277 GAKVYVTNYDQYFSTVAVIDVATNKVTAT 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,084,374 Number of extensions: 1337238 Number of successful extensions: 3118 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3070 Number of HSP's successfully gapped: 12 Length of query: 369 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 261 Effective length of database: 25,922,359 Effective search space: 6765735699 Effective search space used: 6765735699 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)