BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0175.0001.p (785 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1026 [R] COG3973 Superfamily I DNA and RNA helicases 306 9e-83 CAC0603 [R] COG3973 Superfamily I DNA and RNA helicases 199 1e-50 MA2358 [L] COG0210 Superfamily I DNA and RNA helicases 58 6e-08 HP0911 [L] COG0210 Superfamily I DNA and RNA helicases 55 6e-07 Cgl0831 [L] COG0210 Superfamily I DNA and RNA helicases 54 1e-06 >CAC1026 [R] COG3973 Superfamily I DNA and RNA helicases Length = 763 Score = 306 bits (784), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 207/727 (28%), Positives = 354/727 (48%), Gaps = 56/727 (7%) Query: 79 ERHHLQSSKALKKYRKLVVSPYFGRIDFKETADAEAERIYLGIGSLMED-NGSFLIYDWR 137 E+ + + K L+KY + V+PYF RIDF+E E E Y+G L + NG I DWR Sbjct: 59 EKLYNITHKNLEKYNEAKVTPYFARIDFRERKRTEVESYYIGKFGLGDSRNGEEKIIDWR 118 Query: 138 APISSLYYDGAPGPAAYETPGGLVSGTMELKRQFVIDNGTIEVMFDTGVT---------- 187 API+ LYY G G AY+ G V G + LKR+F+I + ++ FD + Sbjct: 119 APIADLYYSGTAGKVAYKIYSGYVDGELYLKRKFLIQDKKLKDAFDDAINEIILKAQGGS 178 Query: 188 ---IGDELLQQVLSHSADDRMKSIVATIQKEQNAIIRNDRSRMLVVQGAAGSGKTSAALQ 244 + DE L+ L + ++K +VATIQKEQN IIR++++ LVVQG+AGSGKT+ AL Sbjct: 179 ENNLVDEFLRINLEKNVSSKLKDVVATIQKEQNDIIRSEKNSALVVQGSAGSGKTTVALH 238 Query: 245 RVAYLLYKYREVLQADQMLLFSPNPLFNSYVSTVLPELGEENMQQTTFQMYLEHRLGQEF 304 R+AYL+YKY + L +L+ +PN +F Y+S VLP LG + Q TF+ LG + Sbjct: 239 RLAYLIYKYNKSLSGKDVLVVAPNRIFLDYISDVLPSLGANTVVQETFEDIALKVLGIK- 297 Query: 305 ELEDVFSQTESLLNAPEGPEAATRRAGI---TYKSSVAFLDVIRRYVNLLEHEGMLFKPL 361 V+++ + L E ++ I +K S+ F ++I +Y+ +E + + + + Sbjct: 298 --SKVYTKDKKLAYIFEDKNEEDKKLVIKTSNFKGSLLFKEMIDKYLKTIEEKDSVVEDI 355 Query: 362 VFQGRAVVSKEEMQQKFYSFDPGIKLANRIDLMTGWLLKKIAAYSVEEREAQWVEDQIEL 421 G + K+E+++ F + + R D + + KI + I + Sbjct: 356 KIAGYVLFDKKEIRRLFCEDMSHLPIDKRKDEIKRYFNLKIK------------DKIINI 403 Query: 422 LDSSDYQRAYNMLRRKGGGRNDSFDDFDMEKTVLARY---------IVSQRLKPLRGWTK 472 D + YN+ R K D + K ++A Y I+ + + + Sbjct: 404 CDKISFTYEYNIARVKKNME----DGVERRKKLIALYDERDNKRNDIIKNAPVVMDNYFE 459 Query: 473 RGRFVDVKGLYSLLFADRELMERLGNGRELPEAWDAICRQTVASLSRNELAYEDATPFLY 532 + D + F +++ L G+ W I + + + + +D LY Sbjct: 460 AWKHTDTAVILDRFFNSKDVYNELAKGKISQSLWKHIKSEFNQNREKKVIDSDDLAAMLY 519 Query: 533 LKELSQGFRTNTQIRHVIVDEVQDYSPFQLEFMRRLFPRAKMTVLGDLNQAI-YAQGEVL 591 +K +G +H+I+DE QDYS FQ ++ L T++GDL Q I Y +G + Sbjct: 520 IKFNIEG-NDKFNFKHIIIDEAQDYSIFQFSALKNLCSSNSFTIVGDLGQGIYYYKG--I 576 Query: 592 GDLGGLVSVYGRENTEVISLTRSYRSTYEIVEFTRSMIPGG----EKIVPFNRRGESPLL 647 D + +N ++L++SYRST EI+EF ++ E P R G+ P + Sbjct: 577 NDWASINKNVFEDNFNYVALSQSYRSTVEIIEFANEVLKKQDIKLEPAKPVLRHGDKPEV 636 Query: 648 TVVDNEEGLLGSIEQDVLNLHSMGYHYVAVICKTAEESARVHAKLQ--NRLPVRLVTKDT 705 + + ++ V L + +A+I KT +E +++ L+ ++ +++ ++ Sbjct: 637 IEFATNKEFVERADEIVERLEKLDKKNIAIIGKTYDECKKINDALRKYSKNKWKVIREND 696 Query: 706 PNFQKGTLVLPAYLAKGVEFDAVIIYDGSEAKY-GRESERKLFYTACTRAMHLLHIYSLG 764 +F+ +V+P+Y+ KG+EFD IIY+ SE Y E ++K Y + TRA+H +I+ G Sbjct: 697 KSFETTKIVIPSYITKGLEFDCSIIYNCSEDNYKDTELDKKTLYVSLTRALHYEYIFFKG 756 Query: 765 QPNHFLP 771 + + + Sbjct: 757 KKSSLIK 763 >CAC0603 [R] COG3973 Superfamily I DNA and RNA helicases Length = 721 Score = 199 bits (507), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 101/207 (48%), Positives = 140/207 (67%), Gaps = 3/207 (1%) Query: 87 KALKKYRKLVVSPYFGRIDFKETADAEAERIYLGIGSLMEDNG-SFLIYDWRAPISSLYY 145 K LK+ L+ SPYFG+IDF+E E E+IY+G + G L+ DWRAPI+SL+Y Sbjct: 95 KRLKELNILLSSPYFGKIDFEEEGLGE-EKIYIGRFGVTPSGGYEPLVVDWRAPIASLFY 153 Query: 146 DGAPGPAAYETPGGLVSGTMELKRQFVIDNGTIEVMFDTGVTIGDELLQQVLSHSADDRM 205 G Y+ P G V+ ++ KRQ++I G +E FD+ V + DE+LQ VLS + D++ Sbjct: 154 TPELGQTRYKAPAGDVTVNVKGKRQYIIKKGKLEGFFDSEVDVKDEILQMVLSKNTGDKL 213 Query: 206 KSIVATIQKEQNAIIRNDRSRMLVVQGAAGSGKTSAALQRVAYLLYKYREVLQADQMLLF 265 + IV TIQ EQ+ IIR +R+++ VV G AGSGKT+ AL RVA+LLY YR +LQ D++L+ Sbjct: 214 RDIVMTIQSEQDNIIRQERNKISVVNGVAGSGKTTIALHRVAFLLYNYRTILQ-DKILIL 272 Query: 266 SPNPLFNSYVSTVLPELGEENMQQTTF 292 PNP+F Y+ VLP LGEEN++Q TF Sbjct: 273 GPNPVFMEYIKNVLPSLGEENVRQETF 299 Score = 118 bits (295), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 62/239 (25%), Positives = 126/239 (52%), Gaps = 5/239 (2%) Query: 525 EDATPFLYLKELSQGFRTNTQIRHVIVDEVQDYSPFQLEFMRRLFPRAKMTVLGDLNQAI 584 +D P +Y+K G + +++HV++DE QDYS Q +++L MT++GD NQ + Sbjct: 486 DDVAPLMYIKIRLDGVKLEREVKHVVIDEAQDYSKLQFIVIKKLTNCLGMTIVGDSNQRL 545 Query: 585 YAQGEVLGDLGGLVSVYGRENTEVISLTRSYRSTYEIVEFTRSMIPGGEKIVPFNRRGES 644 + + + + +++ + E+ L +SYRST EI+E+ + I+P R GE Sbjct: 546 IPKFDEKIAMERMDTIFEDMDFEMFKLDKSYRSTKEIMEYANRFL-NDTSIIPMVRSGEK 604 Query: 645 PLLTVVDNEEGLLGSIEQDVLNLHSMGYHYVAVICKTAEESARVHAKLQNRLPVRLVTKD 704 + +E+ + + + ++ G + VIC+ ++ L+++ + ++ K+ Sbjct: 605 VQSLSISSEDEFVDRVTYAIDDMKKEGIETIGVICENMHDTKYFGELLKSKKYINIIEKE 664 Query: 705 TPNFQKGTLVLPAYLAKGVEFDAVIIYDGSEAKYGRESERKLFYTACTRAMHLLHIYSL 763 + G V+P+Y AKG+EFD VI+ ++ +E++ ++ Y TRA+H L+ + + Sbjct: 665 NQIYTGGETVMPSYFAKGLEFDGVILV----SRENKENKDRVLYVMATRALHKLYDFHI 719 >MA2358 [L] COG0210 Superfamily I DNA and RNA helicases Length = 612 Score = 58.2 bits (139), Expect = 6e-08, Method: Compositional matrix adjust. Identities = 38/115 (33%), Positives = 57/115 (49%), Gaps = 5/115 (4%) Query: 543 NTQIRHVIVDEVQDYSPFQLEFMRRLFP-RAKMTVLGDLNQAIYA-QGEVLGDLGGLVSV 600 N H+IVDE QD + Q E ++ L +TV+ D NQ+IYA +G ++ Sbjct: 259 NDTYTHIIVDEFQDTNYVQFELIKHLTAGEQNITVVADANQSIYAFRGAYSNNIEEFKKQ 318 Query: 601 YGRENTEVISLTRSYRSTYEIVEFTRSMIPGGEKIVPFNRRGESPLLTVVDNEEG 655 +G +V+SL S+RST +I+ +I P +R+ E LL D EG Sbjct: 319 FGISEKDVLSLDTSFRSTNKILRVAHRLISHN---YPEDRKSECLLLKNCDANEG 370 >HP0911 [L] COG0210 Superfamily I DNA and RNA helicases Length = 675 Score = 54.7 bits (130), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 31/94 (32%), Positives = 51/94 (54%), Gaps = 4/94 (4%) Query: 547 RHVIVDEVQDYSPFQLEFMRRLFPRAKMTVLGDLNQAIYA-QGEVLGDLGGLVSVYGREN 605 + V+ DE QD +P Q + + P + V GD +Q+IYA G + + Y +N Sbjct: 206 KEVLCDEFQDTNPLQESILDAINPPSLFCV-GDYDQSIYAFNGADISIISNFTQKY--KN 262 Query: 606 TEVISLTRSYRSTYEIVEFTRSMIPGGEKIVPFN 639 +V +LT++YRS+ EI++ +I E+I P N Sbjct: 263 AQVFTLTKNYRSSKEILDLANQVIQHNERIYPKN 296 >Cgl0831 [L] COG0210 Superfamily I DNA and RNA helicases Length = 763 Score = 53.5 bits (127), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 45/154 (29%), Positives = 74/154 (48%), Gaps = 15/154 (9%) Query: 545 QIRHVIVDEVQDYSPFQLEFMRRLFPR-----AKMTVLGDLNQAIYA-QGEVLGDLGGLV 598 + RHV++DE QD + Q E + L + +++ V+GD +Q+IYA +G + ++ Sbjct: 225 RFRHVLIDEYQDTNHAQYELISTLVGKPDQDPSELCVVGDSDQSIYAFRGATIRNIEEFE 284 Query: 599 SVYGRENTEVISLTRSYRSTYEIVEFTRSMIPGGEKIVPFN-----RRGESPLLTVVDNE 653 + N I L ++YRST I+ ++I E P N GE + V DNE Sbjct: 285 RDFS--NARTILLEQNYRSTQTILSAANAVISQNENRRPKNLWTALGEGEQIIGYVADNE 342 Query: 654 EGLLGSIEQDVLNL--HSMGYHYVAVICKTAEES 685 I ++ NL H M Y +A++ +T S Sbjct: 343 HDEARFIASEIDNLVDHGMSYSDIAIMYRTNNSS 376 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 57,546,489 Number of extensions: 2439753 Number of successful extensions: 9939 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 11643 Number of HSP's successfully gapped: 6 Length of query: 785 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 671 Effective length of database: 25,230,355 Effective search space: 16929568205 Effective search space used: 16929568205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)