BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0174.0001.p (491 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0715 [V] COG1680 Beta-lactamase class C and other penicillin b... 188 2e-47 SA2230 [V] COG1680 Beta-lactamase class C and other penicillin b... 154 4e-37 MA1345 [V] COG1680 Beta-lactamase class C and other penicillin b... 139 2e-32 Rv1730c [V] COG1680 Beta-lactamase class C and other penicillin ... 119 2e-26 MT1771 [V] COG1680 Beta-lactamase class C and other penicillin b... 117 4e-26 >BH0715 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 188 bits (478), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 24/444 (5%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQPVTPQTPFILGSSSKSFTA 93 + I + + Q + + A Sbjct: 34 YVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQA 93 Query: 94 LAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHGRTAFTNT 153 L ++ + + L + + + L + + Sbjct: 94 LDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPD 153 Query: 154 ASTIDELIHGLKN---TPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + V II V+ ++QD S Sbjct: 154 RGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESE 213 Query: 211 LDMKNSYTSTPEAKKHGLATGYKSIFGFMAPFEQPDNPSMLASAYLISSAEDMSHYLVAQ 270 Sbjct: 214 - 272 Query: 271 LNGGKFHDISVAATESVAQMHQPAIKDPATGGEYGMGWEIV-----HGVVQHAGDVESFH 325 Sbjct: 273 --- 329 Query: 326 ADMKL--DGDIGVVVLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSI-TGTYILI 382 Sbjct: 330 - 388 Query: 383 DVISAAVMIMLGVSVFNLIKRKKSFRHTPLRIILFAFCLVLFNAVLPIAVLYGFSHTFAP 442 Sbjct: 389 ------- 441 Query: 443 WSVVFSFLPGLGHLA-FVLSILSL 465 Sbjct: 442 465 >SA2230 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 154 bits (388), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 0/475 (0%), Positives = 0/475 (0%), Gaps = 54/475 (11%) Query: 33 RIDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPDGQ-PVTPQTPFILGSSSKSF 91 ++ + Q L + Sbjct: 42 PGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANA 101 Query: 92 TALAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGI-STFHGRTAF 150 + + E ++ + + ++ D T ++ + A Sbjct: 102 SLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAG 161 Query: 151 TNTASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSP 210 + I + T + + ++ + Q + Sbjct: 162 VHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRY 221 Query: 211 LDMKN-SYTSTPEAKKHGLATGYKSIFGFMAPFEQPD-NPSMLASAYLISSAEDMSHYLV 268 M Y + A Sbjct: 222 HQMLEEQYPLQENGQILL-AFPRL- 279 Query: 269 AQLN-GGKFHDISVAATESVAQMHQ---PAIKDPATGGEYGMGWEIVHG--VVQHAGDVE 322 Sbjct: 280 -------- 331 Query: 323 SFHADMKLD--GDIGVVVLMNAHDYAVRAFKLGMVADGILAIMHGREPVDDAGSITGTY- 379 Sbjct: 332 ----- 386 Query: 380 ----ILIDVISAAVMIMLGVSVFN---------LIKRKKSFRHTPLRIILFAFC----LV 422 Sbjct: 387 -- 444 Query: 423 LFNAVLPIAVLYGFSHTFAPWSVVFSFLPGLGHLAFVLSILSLVIGAAKLIVLMH 477 Sbjct: 445 ------- 492 >MA1345 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 139 bits (349), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 0/370 (0%), Positives = 0/370 (0%), Gaps = 24/370 (6%) Query: 1 MIILLMVAGSVLPLLQPGTAHAEAAPHAVD-TARIDTFVESMLEKLDFPGMAVGIVKGDQ 59 + + A+ AR +E V + Sbjct: 39 GCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIF 98 Query: 60 IIYTQGYGHSGPDG-QPVT-PQTPFILGSSSKSFTALAIMQLVEQGKIDLNAPVQRYLPE 117 + + + + P + + L ++ + R Sbjct: 99 ANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREP 158 Query: 118 FELEDKDASKTILVRHLLNHSSGIS--TFHGRTAFTNTASTIDELIHGLKN--TPLTEPV 173 + + + T + + Sbjct: 159 LAGV-HAYYDILSEAGCEVDIWRTTYYHQMPSH-QAIIDWVTATGLRPWLQDLTESEQQL 216 Query: 174 GSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSPLDMKNSYTSTPEAKK--HGLATG 231 Sbjct: 217 276 Query: 232 Y-KSIFGFMA-PFEQPDNPSMLASAYLISSAEDMSHYLVAQLNGGKFHDISVAATESVAQ 289 Sbjct: 277 --- 333 Query: 290 MHQPAIKD-PATGG-EYGMGWEI---VHGVVQHAGDVESFHADMKL--DGDIGVVVLMNA 342 Sbjct: 334 - 392 Query: 343 HDYAVRAFKL 352 Sbjct: 393 402 >Rv1730c [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 119 bits (297), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 0/351 (0%), Positives = 0/351 (0%), Gaps = 44/351 (12%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPD-GQPVTPQTPFILGSSSKSFT 92 +M+ + V+ D Q P + Sbjct: 60 GIDSSPAMIAEARSALPDCQFVEADIR-NWQPVQALDLIFANASLQWLPDHYELFPHLVS 118 Query: 93 ALAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHGRTAFTN 152 L ++ D L +++ H+ + Sbjct: 119 LLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGC-EVD 177 Query: 153 TASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSPLD 212 T + + + + + E + Sbjct: 178 IWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQI 237 Query: 213 MKNSYTSTPE------AKKHGLATGYKSIFGFMAPFEQPDNPSMLASAYLISSAEDMSHY 266 + Sbjct: 238 LLAFPRLFIV---- 293 Query: 267 LVAQLNGGKF-HDISVAATESVAQMHQPAIKDPATGGEY-----GMG--WEIVHG----- 313 Sbjct: 294 ---- 349 Query: 314 VVQHAGDVESFHAD--MKLDGDIGVVVLMN------------AHDYAVRAF 350 Sbjct: 350 400 >MT1771 [V] COG1680 Beta-lactamase class C and other penicillin binding proteins Length = 252 Score = 117 bits (293), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 0/351 (0%), Positives = 0/351 (0%), Gaps = 44/351 (12%) Query: 34 IDTFVESMLEKLDFPGMAVGIVKGDQIIYTQGYGHSGPD-GQPVTPQTPFILGSSSKSFT 92 + A ++ + + + H + Sbjct: 82 EADIRNWQPVQALDLIFANASLQWLPD-HYELFPHLVSLLNPQGVLAVQMPDNWLEPTHV 140 Query: 93 ALAIMQLVEQGKIDLNAPVQRYLPEFELEDKDASKTILVRHLLNHSSGISTFHGRTAFTN 152 + + + P+ +++ + + + R H T Sbjct: 141 LMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDW-VTA 199 Query: 153 TASTIDELIHGLKNTPLTEPVGSQYQYSNANYDLLGGIIQAVSGESYAEYIQDHVYSPLD 212 Sbjct: 200 259 Query: 213 MKNSYTSTPE------AKKHGLATGYKSIFGFMAPFEQPDNPSMLASAYLISSAEDMSHY 266 Sbjct: 260 ---- 315 Query: 267 LVAQLNGGKF-HDISVAATESVAQMHQPAIKDPATGGEY-----GMG--WEIVHG----- 313 Sbjct: 316 ---- 371 Query: 314 VVQHAGDVESFHAD--MKLDGDIGVVVLMN------------AHDYAVRAF 350 Sbjct: 372 422 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,726,206 Number of extensions: 1513492 Number of successful extensions: 5869 Number of sequences better than 1.0e-05: 61 Number of HSP's gapped: 5786 Number of HSP's successfully gapped: 61 Length of query: 491 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 381 Effective length of database: 25,691,691 Effective search space: 9788534271 Effective search space used: 9788534271 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)