BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0173.0003.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_dnaC [L] COG0305 Replicative DNA helicase 607 e-173 CAC3715 [L] COG0305 Replicative DNA helicase 459 e-129 NMB0885 [L] COG0305 Replicative DNA helicase 395 e-109 YPO0320 [L] COG0305 Replicative DNA helicase 389 e-108 ECs5034 [L] COG0305 Replicative DNA helicase 385 e-107 >BS_dnaC [L] COG0305 Replicative DNA helicase Length = 454 Score = 607 bits (1564), Expect = e-173, Method: Compositional matrix adjust. Identities = 291/442 (65%), Positives = 360/442 (81%), Gaps = 1/442 (0%) Query: 4 DLFFDRIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEES 63 DL DR+PPQN+EAEQAV+GA+ LQ AL A E + +DFY HQ I+ AM+ LG+ Sbjct: 3 DLLNDRLPPQNIEAEQAVLGAIFLQPSALTLASEVLIPDDFYRMSHQKIYNAMLVLGDRG 62 Query: 64 QPIDLVTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTA 123 +P+DLVT+TS L + LE++GG+SYL +A++VPTAAN++YYA+I+EEK++LRRLIRTA Sbjct: 63 EPVDLVTVTSELANTDLLEEVGGISYLTDIANSVPTAANIEYYAKIVEEKSILRRLIRTA 122 Query: 124 TQIVSEGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGG 183 T I +GYT ++V ++LS+AE+ I+E++ R++ S F I+DVL+Q +D +E L+ KG Sbjct: 123 TTIAQDGYTREDEVEDLLSEAEKTIMEVAQRKNTSAFQNIKDVLVQTYDNIEQLYNRKGD 182 Query: 184 TSGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEM 243 +GIPTGF +LD MT GFQRNDLIIVAARPSVGKTAFALNIAQNVA + E+VAIFSLEM Sbjct: 183 ITGIPTGFTELDRMTAGFQRNDLIIVAARPSVGKTAFALNIAQNVATKTDESVAIFSLEM 242 Query: 244 SAPQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDI 303 A QLV RM+CAE N++A +RTG+ +++DW KLTM + SLS S IYIDDTPG+ V++I Sbjct: 243 GAEQLVMRMLCAEGNINAQNLRTGNL-TEEDWGKLTMAMGSLSNSGIYIDDTPGIRVSEI 301 Query: 304 RAKCRRLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALS 363 RAKCRRLK+E GLGMI+IDYLQLIQG G++ +NRQQEVSEISR LK IAREL VPVIALS Sbjct: 302 RAKCRRLKQESGLGMILIDYLQLIQGSGRSKDNRQQEVSEISRELKSIARELQVPVIALS 361 Query: 364 QLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRN 423 QLSRGVEQRQDKRPMMSD+RESGSIEQDADIVAFLYRDDYY+++TE KN AKQRN Sbjct: 362 QLSRGVEQRQDKRPMMSDIRESGSIEQDADIVAFLYRDDYYDKETENKNIIEIIIAKQRN 421 Query: 424 GPVGTVELVFLKNFNKFVNYER 445 GPVGTV L F+K +NKFVN ER Sbjct: 422 GPVGTVSLAFVKEYNKFVNLER 443 >CAC3715 [L] COG0305 Replicative DNA helicase Length = 442 Score = 459 bits (1181), Expect = e-129, Method: Compositional matrix adjust. Identities = 220/441 (49%), Positives = 321/441 (72%), Gaps = 4/441 (0%) Query: 9 RIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEESQPIDL 68 R PQNLEAEQ+V+G+++ + A E + EDFY + HQ+IF AM++L ++ P+DL Sbjct: 6 RAMPQNLEAEQSVLGSMISDKNCIAQAAESLRGEDFYRQNHQIIFNAMLELFQKDIPVDL 65 Query: 69 VTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTATQIVS 128 +TL L+ +LE GG++Y+ ++ ++ + AN+ Y +I+++KA+LR+LI+++T I+ Sbjct: 66 ITLLEHLKSTDKLEASGGITYVTQIRDSIASTANIQSYIKIVKDKAVLRKLIKSSTDIIE 125 Query: 129 EGYTGGEDVANMLSDAERRILEISNRRSGSGFIAIRDVLMQVFDRVELLHQNKGGTSGIP 188 E Y+ +DV +L AE+RI +I+ + S F A+ VL + F+++E L+ NKG +GIP Sbjct: 126 ESYSKQDDVEKVLDSAEKRIFDIAENKVTSDFEAMNVVLERGFEQIEKLYNNKGEITGIP 185 Query: 189 TGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEMSAPQL 248 +GF +LD T+GFQ+ D++++AARPS+GKT FALNIA+ A+RA ++V IFSLEMS QL Sbjct: 186 SGFPELDDKTSGFQKGDMVLIAARPSMGKTTFALNIAEYAALRAGKSVVIFSLEMSKEQL 245 Query: 249 VQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDIRAKCR 308 +++C+EAN+D +RTG+ + D+DW + L++++I+IDDT GV+V ++R+KCR Sbjct: 246 AYKLLCSEANVDMLKLRTGNLE-DEDWDNIARASGPLADAKIFIDDTAGVSVMEMRSKCR 304 Query: 309 RLKKEKGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIALSQLSRG 368 RLK E G+ +IVIDYLQL+ G GE+RQQEVSEISR++K +A+E+ PVIALSQLSR Sbjct: 305 RLKIEHGIDLIVIDYLQLMSGN---GESRQQEVSEISRSIKALAKEIGCPVIALSQLSRA 361 Query: 369 VEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQRNGPVGT 428 EQR D RPM+SDLRESGSIEQDAD+V FLYRD+YYN++TE+KN AKQRNGP GT Sbjct: 362 PEQRNDHRPMLSDLRESGSIEQDADLVMFLYRDEYYNKETEQKNVAECIIAKQRNGPTGT 421 Query: 429 VELVFLKNFNKFVNYERAHNE 449 V L +L ++KF + H E Sbjct: 422 VNLAWLGQYSKFGRLDVIHQE 442 >NMB0885 [L] COG0305 Replicative DNA helicase Length = 468 Score = 395 bits (1014), Expect = e-109, Method: Compositional matrix adjust. Identities = 204/440 (46%), Positives = 299/440 (67%), Gaps = 9/440 (2%) Query: 10 IPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEESQPIDLV 69 +PP ++EAEQ+V+G ++L++ A + V+ EDFY H++IF ++ +L ES+P D++ Sbjct: 19 LPPHSMEAEQSVLGGLMLENPAWDRIADVVSGEDFYRHEHRLIFRSIAKLINESRPADVI 78 Query: 70 TLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTATQIVSE 129 T+ LQ +LE GG YL LA P+AAN+ YA+I+ E++++R+L T+I Sbjct: 79 TVQEDLQRNEELEAAGGFEYLITLAQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARS 138 Query: 130 GYTG-GEDVANMLSDAERRILEI--SNRRSGSGFIAIRDVLMQVFDRVELLHQ--NKGGT 184 Y G D +L +AE ++ +I S +S GF+ + D+L +V R+++L+ N Sbjct: 139 AYNPQGRDAGQLLDEAENKVFQIAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEV 198 Query: 185 SGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEMS 244 +G+PTGF+DLD T+G Q DLIIVA RPS+GKTAF++NIA++VAV + VA+FS+EM Sbjct: 199 TGVPTGFIDLDKKTSGLQPGDLIIVAGRPSMGKTAFSINIAEHVAVEGRLPVAVFSMEMG 258 Query: 245 APQLVQRMICAEANLDANIMRTGDFKSDDDWSKLTMGIQSLSESEIYIDDTPGVTVTDIR 304 QLV RM+ + LD ++++TG + D+ W +L + LS++ +YID+TPG+T ++R Sbjct: 259 GAQLVMRMLGSVGRLDQSVLKTGRLE-DEHWGRLNEAVVKLSDAPVYIDETPGLTALELR 317 Query: 305 AKCRRLKKE--KGLGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIAL 362 A+ RRL ++ LG+IVIDYLQL+ G G++ +NR E+ EISR+LK +A+EL VP+IAL Sbjct: 318 ARARRLARQFNNKLGLIVIDYLQLMAGSGRS-DNRASELGEISRSLKALAKELQVPIIAL 376 Query: 363 SQLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQR 422 SQLSR VE R DKRPMMSDLRESG+IEQDAD++ F+YRD+YYNQD+ K K R Sbjct: 377 SQLSRTVESRTDKRPMMSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHR 436 Query: 423 NGPVGTVELVFLKNFNKFVN 442 NGPVG + L + F KF N Sbjct: 437 NGPVGKIFLTWTGQFTKFDN 456 >YPO0320 [L] COG0305 Replicative DNA helicase Length = 468 Score = 389 bits (998), Expect = e-108, Method: Compositional matrix adjust. Identities = 198/442 (44%), Positives = 303/442 (68%), Gaps = 10/442 (2%) Query: 9 RIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEESQPIDL 68 ++PP +LEAEQ+V+G ++L +E ERV ++DF+ +PH+ IF M +L E S+PIDL Sbjct: 22 KLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPIDL 81 Query: 69 VTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTATQIVS 128 +TL+ L+ KG L+ +GG +YLA+L+ P+AAN+ YA I+ E+A++R +I A +I Sbjct: 82 ITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIAD 141 Query: 129 EGY-TGGEDVANMLSDAERRILEISNRRSGS--GFIAIRDVLMQVFDRVELLHQN-KGGT 184 GY G ++L AE ++ +I+ R+ G ++ +L R+E L+Q G Sbjct: 142 AGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRPHDGV 201 Query: 185 SGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEMS 244 +G+ TGF DLD T G Q++DLIIVAARPS+GKT FA+N+ +N A+ + V IFSLEM Sbjct: 202 TGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNLCENAAMMQDKPVLIFSLEMP 261 Query: 245 APQLVQRMICAEANLDANIMRTGDFKSDDDWSKL--TMGIQSLSESEIYIDDTPGVTVTD 302 Q++ RM+ + +++D +RTG D+DW+++ TMGI + + +YIDD+ G+T T+ Sbjct: 262 GDQIMMRMLASLSHVDQTRIRTGQL-DDEDWARISSTMGIL-MEKRNMYIDDSSGLTPTE 319 Query: 303 IRAKCRRLKKEKG-LGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIA 361 +R++ RR+ +E G L +I+IDYLQL++ +NR E++EISR+LK +A+EL VPV+A Sbjct: 320 VRSRARRIFREHGGLSLIMIDYLQLMRV-PSLSDNRTLEIAEISRSLKALAKELQVPVVA 378 Query: 362 LSQLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQ 421 LSQL+R +EQR DKRP+ SDLRESGSIEQDAD++ F+YRD+ Y++++++K KQ Sbjct: 379 LSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGKQ 438 Query: 422 RNGPVGTVELVFLKNFNKFVNY 443 RNGP+G+V L F +++F NY Sbjct: 439 RNGPIGSVRLKFNGQWSRFDNY 460 >ECs5034 [L] COG0305 Replicative DNA helicase Length = 471 Score = 385 bits (988), Expect = e-107, Method: Compositional matrix adjust. Identities = 200/442 (45%), Positives = 299/442 (67%), Gaps = 10/442 (2%) Query: 9 RIPPQNLEAEQAVIGAVLLQDEALITAMERVNTEDFYDKPHQMIFEAMVQLGEESQPIDL 68 ++PP ++EAEQ+V+G ++L +E ERV +DFY +PH+ IF M +L E PIDL Sbjct: 25 KVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDL 84 Query: 69 VTLTSRLQDKGQLEDIGGVSYLAKLAHAVPTAANVDYYAQIIEEKAMLRRLIRTATQIVS 128 +TL L+ +GQL+ +GG +YLA+L+ P+AAN+ YA I+ E+A++R +I A +I Sbjct: 85 ITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISSYADIVRERAVVREMISVANEIAE 144 Query: 129 EGY-TGGEDVANMLSDAERRILEISNRRSGS--GFIAIRDVLMQVFDRVE-LLHQNKGGT 184 G+ G ++L AE R+ +I+ R+ G I DVL R+E L Q G Sbjct: 145 AGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGV 204 Query: 185 SGIPTGFVDLDHMTNGFQRNDLIIVAARPSVGKTAFALNIAQNVAVRAKETVAIFSLEMS 244 +G+ TG+ DL+ T G Q +DLIIVAARPS+GKT FA+N+ +N A+ + V IFSLEM Sbjct: 205 TGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMP 264 Query: 245 APQLVQRMICAEANLDANIMRTGDFKSDDDWSKL--TMGIQSLSESEIYIDDTPGVTVTD 302 + Q++ R + + + +D +RTG D+DW+++ TMGI L + IYIDD+ G+T T+ Sbjct: 265 SEQIMMRSLASLSRVDQTKIRTGQL-DDEDWARISGTMGIL-LEKRNIYIDDSSGLTPTE 322 Query: 303 IRAKCRRLKKEKG-LGMIVIDYLQLIQGRGKAGENRQQEVSEISRTLKQIARELDVPVIA 361 +R++ RR+ +E G +G+I+IDYLQL++ + +NR E++EISR+LK +A+EL+VPV+A Sbjct: 323 VRSRARRIAREHGGIGLIMIDYLQLMRVPALS-DNRTLEIAEISRSLKALAKELNVPVVA 381 Query: 362 LSQLSRGVEQRQDKRPMMSDLRESGSIEQDADIVAFLYRDDYYNQDTEKKNXXXXXXAKQ 421 LSQL+R +EQR DKRP+ SDLRESGSIEQDAD++ F+YRD+ Y+++++ K KQ Sbjct: 382 LSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQ 441 Query: 422 RNGPVGTVELVFLKNFNKFVNY 443 RNGP+GTV L F +++F NY Sbjct: 442 RNGPIGTVRLTFNGQWSRFDNY 463 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,071,619 Number of extensions: 1362802 Number of successful extensions: 2737 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2679 Number of HSP's successfully gapped: 18 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)