BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0173.0002.p (148 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0863 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 90 1e-18 >STM0863 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 400 Score = 90.1 bits (222), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 8/148 (5%), Positives = 25/148 (16%) Query: 1 MKVIFIKDXXXXXXXXXXXEVSEGYASNFLLPRGLVRPATEGNVKTLENQAAAEKRRKDQ 60 M + + A + + + D+ Sbjct: 1 MTQYASSLRSLAAGSVLLFLFASPVKAEEQTIAPPGVDARAWILMDYASGKVLAEGNADE 60 Query: 61 EKEEAVQLGKKLDELTLTLKAKAGEGGRLFGAITSKQIGETLTAAHGITIDKRKIELSDP 120 + + A + + A G ++ K Sbjct: 61 KLDPASLTKIMTSYVVGQALKAGKIKLTDMVTVGKDAWATGNPALRGSSVMFLKPGDQVS 120 Query: 121 IRHVGTFQVTVKLHTEVKANLTVQVTEE 148 + + + + A + Sbjct: 121 VADLNKGIIIQSGNDACIALADYVAGSQ 148 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.131 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,792,173 Number of extensions: 338347 Number of successful extensions: 555 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 555 Number of HSP's successfully gapped: 1 Length of query: 148 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 51 Effective length of database: 27,191,033 Effective search space: 1386742683 Effective search space used: 1386742683 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 110 (47.0 bits)