BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0172.0011.p (383 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3204 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desul... 281 1e-75 BS_nifZ [E] COG1104 Cysteine sulfinate desulfinase/cysteine desu... 278 1e-74 lin1635 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desu... 271 1e-72 MA3264 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desul... 247 2e-65 SPy0816 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desu... 246 3e-65 >BH3204 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 380 Score = 281 bits (720), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 156/369 (42%), Positives = 223/369 (60%), Gaps = 2/369 (0%) Query: 1 MLYWXXXXXXXXXXEVVQTVEQIMRLHYANPSSLHRAGMEAERLITRAREVCAAALAVQP 60 M+Y EVV + + + NPSSLHR G EAE+L+ + +E A L + Sbjct: 1 MIYLDNSATTRPFMEVVDSYTKASLTFFGNPSSLHRMGTEAEQLLLKTKEQIAGILGISS 60 Query: 61 HEILFTSGATESNNLAIKGAALQYQGRGRHIVTTQLEHPSVYESCRQLQELGWEVSFVAP 120 EI++TSG TE NNLAIKG A Q +GRG H++TT +EH S E+ R L+ G++V+++ P Sbjct: 61 SEIIYTSGGTEGNNLAIKGTAYQLKGRGTHLITTAVEHASTIEAFRSLEAEGFKVTYLKP 120 Query: 121 TGTGIVXXXXXXXXXXXXTVLVSVMHVNNEIGTVQPLREIGQGIKAVNRRTLFHVDGVQG 180 TG V T+LVS++HVNNE G + P+ IG+ +K + FH+D VQG Sbjct: 121 GPTGQVTAEQVKAALTNQTILVSIIHVNNETGIIMPVEGIGELLKGYP-KVRFHIDHVQG 179 Query: 181 YGKLETRLKEWQAELYSLSPHKIRGPRGTGILVVREGTALFPLLTGGSHENGNRAGTENV 240 K+ RL +L + S HK G +GTG + ++G L PL GG E R+GTENV Sbjct: 180 ATKVPLRLAHGLIDLVTFSSHKFHGLKGTGFIYKKDGLRLTPLFHGGGQEGALRSGTENV 239 Query: 241 AGIVASAKAVRMSGERREELVRRLIPLRNRLLHFIAAMPELVLNS-REDGAPHIVHFSYP 299 AG VA+AKA+RM+ E EE +L+ LRN+LL ++ + +V+N+ +E APHIV+FS P Sbjct: 240 AGAVATAKALRMAMELYEERHDKLLHLRNQLLEGLSEIEGVVINTPKELAAPHIVNFSIP 299 Query: 300 GMKGEVLARKLEELGMTVSTRSACSSRLAEPSRILLSMGRDVPAALGGIRISLGDIHTEA 359 MK EV+ + L + + VST+SACS++ +EPS +LL+MG A IR+SL + TE Sbjct: 300 KMKPEVIIQALSKRDIHVSTKSACSTKRSEPSHVLLAMGVGKERANSAIRLSLSYLTTED 359 Query: 360 DVALLEQAL 368 +V+ Q L Sbjct: 360 EVSKALQEL 368 >BS_nifZ [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 381 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 152/364 (41%), Positives = 227/364 (62%), Gaps = 4/364 (1%) Query: 1 MLYWXXXXXXXXXXEVVQTVEQIMRLHYANPSSLHRAGMEAERLITRAREVCAAALAVQP 60 M+Y EV+ EQ ++ NPSSLHR G E E+L+ A+ +L ++ Sbjct: 1 MIYLDNSSTTKPYDEVLNVYEQTSSRYFGNPSSLHRYGAETEQLLQAAKNQIKRSLGLKK 60 Query: 61 HEILFTSGATESNNLAIKGAALQYQGRGRHIVTTQLEHPSVYESCRQLQEL-GWEVSFVA 119 ++I+FTSGATE+NN A+KGAAL G+HI+ T +EHPSV ES QL+EL G+++++++ Sbjct: 61 YDIVFTSGATEANNAALKGAALSKIKTGKHIIATSIEHPSVTESLEQLKELFGFDITYLS 120 Query: 120 PTGTGIVXXXXXXXXXXXXTVLVSVMHVNNEIGTVQPLREIGQGIKAVNRRTLFHVDGVQ 179 G V TVLVS+MHVNNE+G+VQP+ G+ +K + LFHVD VQ Sbjct: 121 VNEDGFVSIEDLKAAIRPDTVLVSMMHVNNEVGSVQPIEAAGEVLKE-HSNILFHVDYVQ 179 Query: 180 GYGKLETRLKEWQAELYSLSPHKIRGPRGTGILVVREGTALFPLLTGGSHENGNRAGTEN 239 G K+ +++ +L S+S HK G +GTG L+V+EGT L PL+TGGS + G RAGTE+ Sbjct: 180 GIYKVPLAIEKAGIDLCSISGHKFHGLKGTGALIVKEGTRLIPLITGGSQQKGIRAGTEH 239 Query: 240 VAGIVASAKAVRMSGERREELVRRLIPLRNRLLHFIAAMPELVLNSRE-DGAPHIVHFSY 298 AG V+ AKA+ ++ + + + ++ ++ ++ + +V+N+ + + APHI++FS Sbjct: 240 TAGAVSLAKAINLASADFDTRLDTMTAVKELFMNRLSEIEGVVINTPQMNSAPHIINFSV 299 Query: 299 PGMKGEVLARKLEELGMTVSTRSACSSRLAEPSRILLSMGRDVPAALGGIRISLGDIHTE 358 PG+K EVL LEE + VST SACS++ +PS++LL MG+ A IRISL T Sbjct: 300 PGIKAEVLLHMLEEQDIFVSTTSACSAKEHKPSKVLLEMGKGEQIAGSSIRISLNYSQT- 358 Query: 359 ADVA 362 +DVA Sbjct: 359 SDVA 362 >lin1635 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 148 Score = 271 bits (694), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 0/378 (0%), Positives = 0/378 (0%), Gaps = 3/378 (0%) Query: 1 MLYWXXXXXXXXXXEVVQTVEQIMRLHYANPSSLHRAGMEAERLITRAREVCAAALAVQP 60 M + ++ + L + + + + A V Sbjct: 1 MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLN 60 Query: 61 HEILFTSGATESNNLAIKGAALQYQGRGRHIVTTQLEHPSVYESCRQLQELGWEVSFVAP 120 + L Q H++ + + Sbjct: 61 QFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGI 120 Query: 121 TGTGIVXXXXXXXXXXXXTVLVSVMHVNNEIGTVQPLREIGQGIKAVNRRTLFHVDGVQG 180 Sbjct: 121 - 179 Query: 181 YGKLETRLKEWQAELYSLSPHKIRGPRGTGILVVREGTALFPLLTGGSHENGNRAGTENV 240 Sbjct: 180 239 Query: 241 AGIVASAKAVRMSGERREELVRRLIPLRNRLLHFIAAMPELVLNSRED-GAPHIVHFSYP 299 Sbjct: 240 - 298 Query: 300 GMKGEVLARKLEELGMTVSTRSACSSRLAEPSRILLSMGRDVPAALGGIRISLGDIHTEA 359 Sbjct: 299 358 Query: 360 DVALLEQALLTAVLSLKK 377 Sbjct: 359 376 >MA3264 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 448 Score = 247 bits (631), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 145/380 (38%), Positives = 215/380 (56%), Gaps = 6/380 (1%) Query: 1 MLYWXXXXXXXXXXEVVQTVEQIMRLHYANPSSLHRAGMEAERLITRAREVCAAAL-AVQ 59 ++Y EV++ + ++ H+ NPSSL+ G E + + +R+ A AL A Q Sbjct: 57 LIYMDHAATTFTKPEVIEAMLPFLKEHFGNPSSLYSIGREGKEAVETSRKKLAKALGAAQ 116 Query: 60 PHEILFTSGATESNNLAIKGAALQYQGRGRHIVTTQLEHPSVYESCRQLQELGWEVSFVA 119 P EI FTSG TES+N AIKG A Q +G+HI+TT +EH +V C L+ G+EV+++ Sbjct: 117 PEEIYFTSGGTESDNWAIKGTAFSRQKKGKHIITTPIEHHAVLYPCEYLETQGFEVTYLP 176 Query: 120 PTGTGIVXXXXXXXXXXXXTVLVSVMHVNNEIGTVQPLREIGQGIKAVNRRTLFHVDGVQ 179 G+V TVL+SVM+ NNEIGT++P+ EIG+ A FH D VQ Sbjct: 177 VDEYGLVNPAEVEASIKKDTVLISVMYANNEIGTIEPILEIGK--IAREHGIPFHTDAVQ 234 Query: 180 GYGK--LETRLKEWQAELYSLSPHKIRGPRGTGILVVREGTALFPLLTGGSHENGNRAGT 237 GK L+ + + ++ SLS HK GP+G G L +REGT + + GG+ E G RAGT Sbjct: 235 VIGKVPLDLQREHKDVDMLSLSSHKFYGPKGIGALYIREGTEIDNYMHGGAQERGKRAGT 294 Query: 238 ENVAGIVASAKAVRMSGERREELVRRLIPLRNRLLHFIAAMPELVLNSR-EDGAPHIVHF 296 ENV GIV KA+ ++ E +L +R RL+ I +P LN E P ++F Sbjct: 295 ENVVGIVGMGKAIELATANIEAHNEKLSKMRARLMAGILEIPYCRLNGHPEKRLPGNLNF 354 Query: 297 SYPGMKGEVLARKLEELGMTVSTRSACSSRLAEPSRILLSMGRDVPAALGGIRISLGDIH 356 S+ ++GE L L+++G+ ST SACSS +EPS +L ++G A G +R++LGD + Sbjct: 355 SFEYIEGESLLLMLDQMGICCSTGSACSSGSSEPSHVLRAIGLAPKTAQGTLRLTLGDAN 414 Query: 357 TEADVALLEQALLTAVLSLK 376 +E D+ + + L V L+ Sbjct: 415 SEEDIDYVLEVLPEVVGKLR 434 >SPy0816 [E] COG1104 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes Length = 373 Score = 246 bits (629), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 142/373 (38%), Positives = 216/373 (57%), Gaps = 12/373 (3%) Query: 1 MLYWXXXXXXXXXXEVVQTVEQIMRLHYANPSSLHRAGMEAERLITRAREVCAAALAVQP 60 M+Y+ E ++T +++ Y NPSSLH+ G A R++ +R+ A L V+ Sbjct: 1 MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRILEASRKQIAGLLGVKS 60 Query: 61 HEILFTSGATESNNLAIKGAALQYQGRGRHIVTTQLEHPSVYESCRQLQELGWEVSFVAP 120 EI FTSG TES N AIKG A + G+HI+ + +EHP+V ES + L G+EVS+ Sbjct: 61 EEIFFTSGGTESANWAIKGIAFEKNAFGKHIIISAIEHPAVSESVKWLLTQGFEVSYAPV 120 Query: 121 TGTGIVXXXXXXXXXXXXTVLVSVMHVNNEIGTVQPLREIGQGIKAVNRRTL-FHVDGVQ 179 T G+V T+L+S+M VNNE+G +QP+R I + N+ T+ FHVD VQ Sbjct: 121 TTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAISNLL--ANQPTITFHVDAVQ 178 Query: 180 GYGKLETRLKEW---QAELYSLSPHKIRGPRGTGILVVREGTALFPLLTGGSHENGNRAG 236 GK+ L ++ + +L S S HK RG G L + G L PLL+GG E R+ Sbjct: 179 AIGKIP--LCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGKRLNPLLSGGGQEQELRST 236 Query: 237 TENVAGIVASAKAVRMSGERREELVRRLIPLRNRLLHFIAAMPELVLNSREDG-APHIVH 295 TENVAGI + AKA+R+ E++ ++ +L +R+ + ++A P++ + S ++G AP+I+ Sbjct: 237 TENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKSLSAYPDVTVFSAQEGFAPNILT 296 Query: 296 FSYPGMKGEVLARKLEELGMTVSTRSACSSRLAEPSRILLSMGRDVPAALGGIRISLGDI 355 F G++GEV+ E+ + +ST SACSS+ EP+ L++MG V A +RISL D Sbjct: 297 FGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGEPAGSLVAMGIPVKTAQTAVRISLDD- 355 Query: 356 HTEADVALLEQAL 368 + D+ +EQ L Sbjct: 356 --DNDMGQVEQFL 366 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,049,167 Number of extensions: 1082887 Number of successful extensions: 4975 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 4794 Number of HSP's successfully gapped: 79 Length of query: 383 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 275 Effective length of database: 25,922,359 Effective search space: 7128648725 Effective search space used: 7128648725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)