BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0172.0001.p (455 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0495 [S] COG2606 Uncharacterized conserved protein 327 3e-89 XF0959 [T] COG1875 Predicted ATPase related to phosphate starvat... 147 3e-35 XF0960 [T] COG1875 Predicted ATPase related to phosphate starvat... 108 2e-23 CC2644 [T] COG1702 Phosphate starvation-inducible protein PhoH, ... 97 4e-20 CC0055 [T] COG1702 Phosphate starvation-inducible protein PhoH, ... 96 9e-20 >STM0495 [S] COG2606 Uncharacterized conserved protein Length = 159 Score = 327 bits (838), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 27/444 (6%) Query: 13 MKKIFVLDTNVLLHDPNSIFAFKEHEVVIPAVVLEEIDSKKRNADEIGRNARTVSRLLDG 72 M L + + +E V+ ++ + A Sbjct: 1 MTPAVKLLEKNKIPFNIHTYDHDPNETNFGDEVVRKLGLNADQVYKTLLVAVNGDMKQLA 60 Query: 73 LRELGHLHSGVVLEHGGTLKVELNHRSFVKVQEMFGEVSNDNRILAVALNYLHEENEKAE 132 + + + L + + V + L Sbjct: 61 VAVTPVAGQLDLKKVAKALGAKKVDMADPMVAQRTTGYLVGGISPLGQKKRLPTLI---- 116 Query: 133 PRPVVLVSKDVLVRIKADVLGITPEDYLSDRTGDLNELYAGYQSLMVHPSLIDEYYSHRS 192 + V L I + + Sbjct: 117 -DAPARTFATIYVSGGKRGLDIELAADDLAAI-LGAKFADIARR------- 167 Query: 193 LSVK-QLALSYPLYPHEFVIMKDEIGTGKSALLKVNSDASRLEPLYLGNDAVWGISARNA 251 Sbjct: 168 ----------- 216 Query: 252 QQRMALELLLNDDIPLVXXXXXXXXXXXXXXXXXXXFKVEDEHKYKKLLIARPVVPMGKD 311 Sbjct: 217 276 Query: 312 IGYLPGEKDEKLRPWMQPIYDNLEFLFDTKKSGDIDKILMGLGSIQVEALTYIRGRSIPS 371 Sbjct: 277 -- 334 Query: 372 QFIIIDEAQNLSRHEVKTIVSRAGEGSKVILMGDPEQIDHPYLDAASNGLSYIVEKFKQQ 431 Sbjct: 335 394 Query: 432 GISGHITLEKGERSHLAQLAADLL 455 Sbjct: 395 418 >XF0959 [T] COG1875 Predicted ATPase related to phosphate starvation-inducible protein PhoH Length = 553 Score = 147 bits (372), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 10/167 (5%), Positives = 37/167 (22%), Gaps = 3/167 (1%) Query: 292 DEHKYKKLLIARPVVPMGKDIGYLPGEKDEKLRPWMQPIYDNLEFLFDTKKSGDIDKILM 351 H +K + + + + + R + + ++S ++L Sbjct: 32 SLHHSQKSFVVSNQLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLD 91 Query: 352 G---LGSIQVEALTYIRGRSIPSQFIIIDEAQNLSRHEVKTIVSRAGEGSKVILMGDPEQ 408 + + + + + + T ++ + G Sbjct: 92 SARKTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFA 151 Query: 409 IDHPYLDAASNGLSYIVEKFKQQGISGHITLEKGERSHLAQLAADLL 455 + A ++ +T + A + Sbjct: 152 QPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIA 198 >XF0960 [T] COG1875 Predicted ATPase related to phosphate starvation-inducible protein PhoH Length = 553 Score = 108 bits (269), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 11/176 (6%), Positives = 33/176 (18%), Gaps = 16/176 (9%) Query: 14 KKIFVLDTNVLLHDPNSIFAFKEHEVVIPAVVLEEIDSKKRNADEIGRNARTVSRLLDGL 73 ++ L VL + + ++ G T +L Sbjct: 5 IRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSNQLREQQGELTSTWDLMLQTR 64 Query: 74 ---RELGHLHSGVVLEHGGTLKVELNH---RSFVKVQEM-----FGEVSNDNRILAVALN 122 KVEL ++ + + + ++ Sbjct: 65 INLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAATHYKKFKSMAPLPEMVATSRNID 124 Query: 123 YLHEENEKAEPRPVVLVSKDVLVRIKADVLGITPEDYLSDRT-GDLNELYAGYQSL 177 +N + + + + + + + Sbjct: 125 -EKYKNYYTA---LTELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLY 176 >CC2644 [T] COG1702 Phosphate starvation-inducible protein PhoH, predicted ATPase Length = 553 Score = 97.4 bits (241), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 9/134 (6%), Positives = 23/134 (17%), Gaps = 7/134 (5%) Query: 295 KYKKLLIARPVVPMGKDIGYLPGEKDEKLRPWMQPIYDNLEFLFDTKKSGDIDKILMGLG 354 K + + Y L L D Sbjct: 95 KTLAQAATHYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDY----GNTGAYF 150 Query: 355 SIQVEALTYIRGRSIPSQFIIIDEAQNLSRHEVKTIVSRAGEGSKVILMGDPEQIDHPYL 414 + + + G + + ++ + A VI + + + Sbjct: 151 AQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILL---V 207 Query: 415 DAASNGLSYIVEKF 428 + Sbjct: 208 AWYGIRRMLLTPLA 221 >CC0055 [T] COG1702 Phosphate starvation-inducible protein PhoH, predicted ATPase Length = 553 Score = 96.3 bits (238), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 10/138 (7%), Positives = 31/138 (22%), Gaps = 8/138 (5%) Query: 298 KLLIARPVVPMGKDIGYLPGEKDEKLRPWMQPIYDNLEFLFDTKKSGDIDKILMGLGSIQ 357 A P + + + + + D + + + Sbjct: 144 GNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYR---DIVTDNADDYRFAQWQLAV-IAL 199 Query: 358 VEALTYIRGRSIPSQFIIIDEAQNLSRHEVKTIVSRAGEGSKVILMGDPEQIDHPYLDAA 417 V L + + ++ A+ ++ + A + + + Sbjct: 200 VVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQ--SVSHM 257 Query: 418 SNGLSYIVEKFKQQGISG 435 L+ V + S Sbjct: 258 QRSLTDTVTHV--REGSD 273 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,627,648 Number of extensions: 1331462 Number of successful extensions: 5835 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 5815 Number of HSP's successfully gapped: 17 Length of query: 455 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 345 Effective length of database: 25,691,691 Effective search space: 8863633395 Effective search space used: 8863633395 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)