BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0171.0007.p (245 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA1999 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family 184 8e-47 PAE2959 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family 170 2e-42 AF0112 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family 153 2e-37 STM0490 [I] COG0657 Esterase/lipase 149 4e-36 PAE3500 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family 146 3e-35 >SA1999 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family Length = 243 Score = 184 bits (468), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 96/240 (40%), Positives = 136/240 (56%), Gaps = 2/240 (0%) Query: 2 DPLQTLVSWIKDSGSIVFFGGAGTSTESGIPDFRSAAGLYQTQHNSPYPPEVMLSRSFFM 61 + L+TL I S I FF GAG S SG+PDFRS GL+ PE +LSR + Sbjct: 3 NDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGLFDEISKDGLSPEYLLSRDYLE 62 Query: 62 SSPDIFFDFYRSKMIHPEAKPNGAHQLLAQLERDGKLKAVITQNIDGLHQLAGSRRVLEL 121 P+ F +F +++ + KPN H +A+LER+ + VITQNIDGLH AGS+ V EL Sbjct: 63 DDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQSLGVITQNIDGLHSDAGSQHVDEL 122 Query: 122 HGSIHRNHCMDCSRYYGLDQVLASEAGVPRCPECGGIIKPDVVLYEEELDHDXXXXXXXX 181 HG+++R +C C + Y V+ + + C CGG I+PD+VLY E LD Sbjct: 123 HGTLNRFYCNACHKSYTKSDVI--DRTLKHCDNCGGAIRPDIVLYGEMLDQPTIIRALNK 180 Query: 182 XXXXXXXXXGGTSLTVQPAASLITYFRGRHTVLLNGDPTPYDHQADLIITDRIGAVMGKI 241 G+SL VQPAA LI++F+G + +++N D TPYD A L+I D + +V+ + Sbjct: 181 IEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIINKDRTPYDSDATLVIHDDMVSVVKSL 240 >PAE2959 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family Length = 254 Score = 170 bits (430), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 102/248 (41%), Positives = 133/248 (53%), Gaps = 10/248 (4%) Query: 2 DPLQTLVSWIKDSGSIVFFGGAGTSTESGIPDFRSAAGLYQTQHNSPYPPEVMLSRSFFM 61 D L + S I S V GAG ST SGIPDFR G+++ PE S+F Sbjct: 8 DELDEVASLIARSSCNVALTGAGVSTASGIPDFRGPQGVWRRVD-----PE-KFEISYFY 61 Query: 62 SSPDIFFDFYRSKMIHP-EAKPNGAHQLLAQLERDGKLKAVITQNIDGLHQLAGSRRVLE 120 ++PD +D + ++ KPN AH LA++ER GKL AVITQN+D LHQ AGS+ V+E Sbjct: 62 NNPDEVWDLFVKYLLPAFNVKPNPAHYALAEMERLGKLCAVITQNVDRLHQAAGSKNVIE 121 Query: 121 LHGSIHRNHCMDCSRYYGLDQVLA-SEAGVPRCPECGGIIKPDVVLYEEELDHDXXXXXX 179 LHG++ C +C Y L + L ++G PRCP+CGG+IKPDVV + E L D Sbjct: 122 LHGALEYAVCTNCGSKYALAEALKWRKSGAPRCPKCGGVIKPDVVFFGEPLPQDALREAF 181 Query: 180 XXXXXXXXXXXGGTSLTVQPAAS--LITYFRGRHTVLLNGDPTPYDHQADLIITDRIGAV 237 GTSL V PA L+ RG V++N D T YD AD II R V Sbjct: 182 MLAEMAEVFMAIGTSLAVYPANQLPLVAKKRGAKLVIINADETYYDFFADYIIRGRAEEV 241 Query: 238 MGKIQELL 245 + K+ + L Sbjct: 242 LPKLLDRL 249 >AF0112 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family Length = 253 Score = 153 bits (387), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 97/247 (39%), Positives = 129/247 (52%), Gaps = 10/247 (4%) Query: 2 DPLQTLVSWIKDSGSIVFFGGAGTSTESGIPDFRSAAGLYQTQHNSPYPPEVMLSRSFFM 61 D ++ + S V F GAG S ESGIP FR GL++ Y PE + S S F Sbjct: 3 DEIRKAAEILAKSKHAVVFTGAGISAESGIPTFRGEDGLWRK-----YDPEEVASISGFK 57 Query: 62 SSPDIFFDFYRSKMIHPEAKPNGAHQLLAQLERDGKLKAVITQNIDGLHQLAGSRRVLEL 121 +P F++F A+PN AH +A+LER G +KAVITQNID LHQ AGSRRVLEL Sbjct: 58 RNPRAFWEFSMEMKDKLFAEPNPAHYAIAELERMGIVKAVITQNIDMLHQRAGSRRVLEL 117 Query: 122 HGSIHRNHCMDCSRYYGLDQVLA--SEAGVPRCPECGG-IIKPDVVLYEEELDHDXXXXX 178 HGS+ + C+DC Y + + ++ +PRC +CG +KP VVL+ E L Sbjct: 118 HGSMDKLDCLDCHETYDWSEFVEDFNKGEIPRCRKCGSYYVKPRVVLFGEPLPQRTLFEA 177 Query: 179 XXXXXXXXXXXXGGTSLTVQPAASL--ITYFRGRHTVLLNGDPTPYDHQADLIITDRIGA 236 G+SL V PAA L I G +++N +PT D D+ I + G Sbjct: 178 IEEAKHCDAFMVVGSSLVVYPAAELPYIAKKAGAKMIIVNAEPTMADPIFDVKIIGKAGE 237 Query: 237 VMGKIQE 243 V+ KI E Sbjct: 238 VLPKIVE 244 >STM0490 [I] COG0657 Esterase/lipase Length = 323 Score = 149 bits (376), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 7/231 (3%), Positives = 34/231 (14%), Gaps = 13/231 (5%) Query: 4 LQTLVSWIKDSGSIVFFGGAGTSTESGIPDFRSAAGLYQTQHNSPYPPEVMLSRSFFMSS 63 + + + + + + + Sbjct: 3 PENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFW------NADAP 56 Query: 64 PDIFFDFYRSKMIHP--EAKPNGAHQLLAQLERDGKLKAVITQNIDGLHQLAGSRRVLEL 121 + + I N+D ++ Sbjct: 57 SMTTRTCAVPT-PYGDVTTRLYSPQPTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTG 115 Query: 122 HGSIHRNHCMDCSRYYGLDQVLAS--EAGVPRCPECGGIIKPDVVLYEEELDHDXXXXXX 179 I ++ + Y + + + + + + Sbjct: 116 CTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIGFAGDSAGAMLALASA 175 Query: 180 XXXXXXXXXXXGGTSLTVQPAAS--LITYFRGRHTVLLNGDPTPYDHQADL 228 ++ + + R +G + Sbjct: 176 LWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEK 226 >PAE3500 [K] COG0846 NAD-dependent protein deacetylases, SIR2 family Length = 249 Score = 146 bits (368), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 88/237 (37%), Positives = 125/237 (52%), Gaps = 12/237 (5%) Query: 11 IKDSGSIVFFGGAGTSTESGIPDFRSAAGLYQTQHNSPYPPEVMLSRSFFMSSPDIFFDF 70 + S V F GAG S ESG+P FR GL++ Y PE + + F P + + + Sbjct: 7 LASSRHCVVFTGAGISAESGVPTFRGPGGLWER-----YKPEELATPEAFARDPALVWRW 61 Query: 71 Y--RSKMIHPEAKPNGAHQLLAQLERDGKLKAVITQNIDGLHQLAGSRRVLELHGSIHRN 128 Y R ++I+ A+P+ H +A+LE G ++ VITQN+DGLHQ AGSR V+ELHGSI R Sbjct: 62 YKWRQEVIY-NARPSPGHYAIAELEAMGVVRGVITQNVDGLHQRAGSRLVVELHGSIWRA 120 Query: 129 HCMDCSRYYGLDQVLASEAGVPRCPECGGIIKPDVVLYEEELDHDXXXXXXXXXXXXXXX 188 C+ C Y LD+ + E P C +CGG+++PDVV + E L + Sbjct: 121 RCVKCGSVYILDKPV--EEVPPLCRKCGGLLRPDVVWFGEPLPQEAWRAAVELASVSDVL 178 Query: 189 XXGGTSLTVQPAASL--ITYFRGRHTVLLNGDPTPYDHQADLIITDRIGAVMGKIQE 243 GTS V PAA + I G V +N +P+ AD+ I R G V+ ++ E Sbjct: 179 LVVGTSGVVYPAAYIPRIAKEAGARVVEINVEPSAITPIADVFIQGRAGEVLPRLVE 235 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,710,951 Number of extensions: 691219 Number of successful extensions: 5799 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 5763 Number of HSP's successfully gapped: 18 Length of query: 245 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 141 Effective length of database: 26,383,695 Effective search space: 3720100995 Effective search space used: 3720100995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)