BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0027.p (350 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3116 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 285 1e-76 RSp1389 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 184 2e-46 XF2024 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 173 4e-43 mlr4314 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 171 1e-42 AGc4888 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 163 4e-40 >BH3116 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 285 bits (728), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 0/316 (0%), Positives = 0/316 (0%), Gaps = 17/316 (5%) Query: 26 RYNNIDNVSTDKTLKQLRQWREERRMKKKDYSYKVPNLPPK-LDYLSENRLDTTVTWIGH 84 ++ + + + + ++ +Y + P L + +T I Sbjct: 4 WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDS 63 Query: 85 STFLLQYEGVNIITDPVWARRLGFEKRITPPGIPLSGIPPVDLILISHSHYDHLHLASIR 144 S ++ + + + + + + L + L + + Sbjct: 64 SPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQ 123 Query: 145 KLYKAGTTLVVPVGLKRKMVRKGFVNCLEMQWWEEIKVGAVKLTFVPTQHWTRRTPFDTN 204 + A + ++R+ E G + ++ + T Sbjct: 124 GV-LAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTT 182 Query: 205 TSHWGGFVLEPAEPRKHPQGDGGSRKLPPNLYFAGDSGFFPGFKEIGRRFKLHVALMPIG 264 H + + + F M Sbjct: 183 YYHQMPSHQAIIDWVT---------------ATGLRPWLQDLTESEQQLFLKRYHQMLEE 227 Query: 265 AYEPEWFMSSQHVTPEEAIQAFLDVGAELMIPMHFGTFRLADDTAREALDRMEQARLAQG 324 Sbjct: 228 287 Query: 325 IAGERIRTLAYGETLI 340 Sbjct: 288 303 >RSp1389 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 184 bits (466), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 0/335 (0%), Positives = 0/335 (0%), Gaps = 36/335 (10%) Query: 24 RVRYNNIDNVSTDKTLKQLRQWREERRMK-KKDYSYKVPNLPPKLDYLSENRLDTTVTWI 82 + + P S + + + Sbjct: 19 PAVELLARVPLENVEYVADL---GCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSAL 75 Query: 83 GHSTFLLQYEGVNIITDPVWARR-----LGFEKRITPPGIPLSGIPPVDLILISHSHYDH 137 F+ + +S + P ++ + Sbjct: 76 PDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWL 135 Query: 138 LHLASIRKLYK---AGTTLVVPVGLKRKMVRK--------GFVNCLEMQWWEEIKVGAVK 186 + + + + Sbjct: 136 EPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIID 195 Query: 187 LTFVPTQHWTRRTPFDTNTSHWGGFVLEPAEPRKHPQGDGGSRKLPPNLYFAGDSGFFPG 246 + ++ + + + + Sbjct: 196 WVTATGLRPWLQDLTESEQQLFLKRYH------QMLEE---------QYPLQENGQILLA 240 Query: 247 FKEIGRRF-KLHVALMPIGAYEPEWFMSSQHVTPEEAIQAFLDVGAELMIPMHFGTFRLA 305 F + Sbjct: 241 FPRLFIVAR 300 Query: 306 DDTAREALDRMEQARLAQGIAGERIRTLAYGETLI 340 Sbjct: 301 335 >XF2024 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 173 bits (439), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 0/335 (0%), Positives = 0/335 (0%), Gaps = 51/335 (15%) Query: 25 VRYNNIDNVSTDKTLKQLRQWREERRMKKKDYSYKVPNLPPKLDYLSENRLDT------- 77 + + ++ + N L Sbjct: 18 RPAVELLARVPLENVEYVADLGCGPG-----------NSTALLQ-QRWPAARITGIDSSP 65 Query: 78 -TVTWIGHSTFLLQYEGVNIITDPVWARRLG-----FEKRITPPGIPLSGIPPVDLILIS 131 + + Q+ +I + + + + Sbjct: 66 AMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGV 125 Query: 132 HSHYDHLHLASIRKLYKA-GTTLVVPVG---LKRKMVRKGFVNCLEMQWWEEIKVG--AV 185 + + + V + E E+ + Sbjct: 126 LAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSE--AGCEVDIWRTTY 183 Query: 186 KLTFVPTQHWTRRTPFDTNTSHWGGFVLEPAEPRKHPQGDGGSRKLPPNLYFAGDSGFFP 245 Q Q L Sbjct: 184 YHQMPSHQAIIDWVTATGLRPWLQDLTESE------QQL---------FLKRYHQMLEEQ 228 Query: 246 G--FKEIGRRFKL-HVALMPIGAYEPEWFMSSQHVTPEEAIQAFLDVGAELMIPMHFGTF 302 + Sbjct: 229 YPLQENGQILLAFP 288 Query: 303 RLADDTAREALDRMEQARLAQGIAGERIRTLAYGE 337 Sbjct: 289 323 >mlr4314 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 171 bits (434), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 0/282 (0%), Positives = 0/282 (0%), Gaps = 36/282 (12%) Query: 76 DTTVTWIGHSTFLLQYEGVNIITDPVWARR-----LGFEKRITPPGIPLSGIPPVDLILI 130 W L + ++ P + +L+ Sbjct: 83 ADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLM 142 Query: 131 SHSHYDHLHLASIRKLYKAGTTLVV-PVGLKRKMVRKGF--VNCLEMQWWEEIKVGA-VK 186 ++ + R+ V I Sbjct: 143 REVAWEQNYPDRGREPLAGVHAYYDILSE-AGCEVDIWRTTYYHQMPSHQAIIDWVTATG 201 Query: 187 LTFVPTQHWTRRTPFDTNTSHWGGFVLEPAEPRKHPQGDGGSRKLPPNLYFAGDSGFFPG 246 L H P Q N Sbjct: 202 LRPWLQDLTESEQQLFLKRYHQMLEEQYP------LQE---------NGQILLAFPRLFI 246 Query: 247 F-------KEIGRRFKLHVALMPIGAYEPEWFMSSQHVTPEEAIQAFLDVGAELMIPMHF 299 Sbjct: 247 V---- 302 Query: 300 GTFRLADDTAREALDRMEQARLAQGIAGERIRTLAYGETLIV 341 Sbjct: 303 344 >AGc4888 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Length = 252 Score = 163 bits (412), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 0/278 (0%), Positives = 0/278 (0%), Gaps = 29/278 (10%) Query: 76 DTTVTWIGHSTFLLQYEGVNIITDPVWARR---LGFE--KRITPPGIPLSGIPPVDLILI 130 +Q + V R + P + + Sbjct: 115 HLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGC 174 Query: 131 SHSHYDHLHLASIRKLYKAGTTL-VVPVGLKRKMVRKGFVNCLEMQ---WWEEIKVGAVK 186 + + + + + + ++ Sbjct: 175 EVDIWRTTYYHQMPSHQAIIDWVTATGLR-PWLQDLTE-SEQQLFLKRYHQMLEEQYPLQ 232 Query: 187 LTFVPTQHWTRRTPFDTNTSHWGGFVLEPAEPRKHPQGDGGSRKLPPNLYFAGDSGFFPG 246 Sbjct: 233 --------------- 277 Query: 247 FKEIGRRFK---LHVALMPIGAYEPEWFMSSQHVTPEEAIQAFLDVGAELMIPMHFGTFR 303 Sbjct: 278 337 Query: 304 LADDTAREALDRMEQARLAQGIAGERIRTLAYGETLIV 341 Sbjct: 338 375 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,940,302 Number of extensions: 1222511 Number of successful extensions: 2609 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 2552 Number of HSP's successfully gapped: 24 Length of query: 350 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 243 Effective length of database: 26,037,693 Effective search space: 6327159399 Effective search space used: 6327159399 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)