BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0024.p (289 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yeaB [P] COG0053 Predicted Co/Zn/Cd cation transporters 270 2e-72 BS_ydbO [P] COG0053 Predicted Co/Zn/Cd cation transporters 223 4e-58 lin2333 [P] COG0053 Predicted Co/Zn/Cd cation transporters 219 3e-57 CAC0087 [P] COG0053 Predicted Co/Zn/Cd cation transporters 210 2e-54 L106965 [P] COG0053 Predicted Co/Zn/Cd cation transporters 200 3e-51 >BS_yeaB [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 192 Score = 270 bits (691), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 0/283 (0%), Positives = 0/283 (0%) Query: 5 YDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRI 64 V LT L G Sbjct: 4 RSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQPGLLLTQRSIHLRKHAGQVA 63 Query: 65 SRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXX 124 D A A I + V G + TP + Sbjct: 64 FPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLPPVDSVTGYQVTPVVGIIPPDL 123 Query: 125 XXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAA 184 + Q + + + +G+ Sbjct: 124 PYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDSHRVWLSWYEQYFVWGMTAGIIRE 183 Query: 185 AVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXX 244 Sbjct: 184 243 Query: 245 XXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 Sbjct: 244 286 >BS_ydbO [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 309 Score = 223 bits (567), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 15/267 (5%), Positives = 40/267 (14%) Query: 6 DDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGLRIS 65 + + ++ G A + A + + Sbjct: 5 ERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIVGMLPEGNWITRAPTLRRKAI 64 Query: 66 RKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXXXXX 125 D + A L + + S Sbjct: 65 LLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRMKYSSIFNYPTLSWADIGVIGWL 124 Query: 126 XXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDSAAA 185 + R + A++ K+ A + + + A Sbjct: 125 VDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQRQGFEACMALAQGSEAQKQMLQDAI 184 Query: 186 VAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXXXXX 245 L +S + + R + Sbjct: 185 NRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTNDELRQRFVDNTVPQVEMLGMTVPDPD 244 Query: 246 XXXXXXXXXXXXXMQGHEISDSIEERM 272 + + + Sbjct: 245 LHFDTESGHYRFGEIDWQEFNEVINGR 271 >lin2333 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 289 Score = 219 bits (559), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 115/285 (40%), Positives = 167/285 (58%) Query: 3 DIYDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGL 62 D Y+D++K E+ K+ +G+ S ALLADG NN TDIVAS+A+LIGL Sbjct: 2 DGYNDLKKAEKAAFLSIFAYLFLSVLKIAAGHFGNSDALLADGLNNTTDIVASVALLIGL 61 Query: 63 RISRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXX 122 RISR PPD+DH+YGH R ET+++L+AS IM +VG+QVI ++ + E P + + Sbjct: 62 RISRIPPDADHSYGHRRTETISSLIASIIMFLVGVQVIWSSIVHIIEKEFTAPSMLTAIV 121 Query: 123 XXXXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDS 182 Y+YN RLA+++++ A+ AAA DN SDA VS GA +GI+GA G+PW+DS Sbjct: 122 ALFSGVFMYAIYLYNHRLAKKLDSQAVRAAAYDNRSDAFVSFGAFIGIIGAVLGVPWLDS 181 Query: 183 AAAVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXX 242 A VG+LI+ TA IF D+ + LTDGFD +L + IA P V+ + D+KAR Sbjct: 182 VTAFLVGVLIVYTAVKIFYDAAHTLTDGFDVSKLETIHDLIASVPDVKKVIDIKARMNGN 241 Query: 243 XXXXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 ++ HEI++ +E+++ + VH+EP Sbjct: 242 RIWIDATIAVDPDLNVVKSHEITEIVEQKIRNEYEGAFTLVHIEP 286 >CAC0087 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 292 Score = 210 bits (535), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 110/285 (38%), Positives = 163/285 (57%) Query: 3 DIYDDIRKGERGXXXXXXXXXXXXXFKLISGYVFGSSALLADGFNNLTDIVASLAVLIGL 62 D Y +++ ERG FK GY F S AL ADG N+ TDI+A++A+++GL Sbjct: 3 DNYKNLKIAERGVRISLIIYVVLSIFKFFMGYFFKSEALKADGINSSTDIIAAIAIIVGL 62 Query: 63 RISRKPPDSDHTYGHFRAETVAALLASFIMAMVGIQVIVEAVRSLFEGAKETPQLWSXXX 122 +I+RKP D +HTYGH RAET+++LLAS IM VG+ V+ V++ E P + + Sbjct: 63 KIARKPADKEHTYGHLRAETISSLLASLIMIAVGVDVVYNGVKNTIFLRNEKPDIKAAIV 122 Query: 123 XXXXXXXXXXXYIYNKRLARQINNGALLAAAKDNFSDAMVSIGAAVGIVGAQFGLPWIDS 182 Y YN+ +A +I++ AL+ ++KDN SD+MV G+A GI +QFG PW+DS Sbjct: 123 SLICASVVYTLYRYNRSVADKIHSSALMVSSKDNLSDSMVGFGSAFGIFASQFGFPWMDS 182 Query: 183 AAAVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLLDLRSTIARTPGVEGIKDLKARXXXX 242 AV VG++I KT + IFR++ LTD FD ++L ++ I GV IKD++AR Sbjct: 183 ITAVLVGIIICKTGFGIFREAVNNLTDAFDPEKLENITKIIKDIDGVSDIKDIRARVHGN 242 Query: 243 XXXXXXXXXXXXXXXXMQGHEISDSIEERMSTLHNIMNVQVHVEP 287 + HEI++++E+ + NI N +HVEP Sbjct: 243 SILLDAIILVDPGLSVRESHEITEAVEKILKEEFNIRNAIIHVEP 287 >L106965 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 425 Score = 200 bits (508), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 100/250 (40%), Positives = 151/250 (60%) Query: 38 SSALLADGFNNLTDIVASLAVLIGLRISRKPPDSDHTYGHFRAETVAALLASFIMAMVGI 97 SSAL A+GFNN+TDI+ ++A+LIGLR++R P DSDH YGH++ E++A+L++SFIM VG Sbjct: 39 SSALQANGFNNVTDIMGNIAILIGLRLARIPADSDHVYGHWKIESIASLISSFIMFFVGF 98 Query: 98 QVIVEAVRSLFEGAKETPQLWSXXXXXXXXXXXXXXYIYNKRLARQINNGALLAAAKDNF 157 +V+ E V +L G +E Y+YN+ LA++ N+ AL AA+KDN Sbjct: 99 EVLRETVMTLLSGKEEAIDPLGALVGLFSAFVMLGVYLYNRALAKKTNSQALSAASKDNL 158 Query: 158 SDAMVSIGAAVGIVGAQFGLPWIDSAAAVAVGLLILKTAWDIFRDSTYRLTDGFDQDRLL 217 SDA+ SIG V I+ + G W+D+A AV + ILKTA+DIF +S + L+DGFD++ + Sbjct: 159 SDAVTSIGTVVAILASSVGWEWLDTAMAVVICGFILKTAYDIFHESVFSLSDGFDENLVE 218 Query: 218 DLRSTIARTPGVEGIKDLKARXXXXXXXXXXXXXXXXXXXXMQGHEISDSIEERMSTLHN 277 + R I P V+G+K ++ R + HE ++ IE+ + + Sbjct: 219 EYREAICLVPKVKGVKLVRGRSYGSNIFLDVVVEMSPDLSVFESHEATEFIEKMLIDNYG 278 Query: 278 IMNVQVHVEP 287 + +V VHVEP Sbjct: 279 VYDVDVHVEP 288 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,844,956 Number of extensions: 570859 Number of successful extensions: 1500 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 1457 Number of HSP's successfully gapped: 34 Length of query: 289 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 184 Effective length of database: 26,268,361 Effective search space: 4833378424 Effective search space used: 4833378424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)