BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0015.p (289 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1848 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 345 6e-95 MA3542_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting p... 132 5e-31 MA1989_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting p... 129 6e-30 all1716_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting ... 124 2e-28 CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting pr... 122 6e-28 >all1848 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 276 Score = 345 bits (884), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 158/256 (61%), Positives = 201/256 (78%) Query: 32 GVHRFYGYDFRNYALPSLKRRIWHHVHAENVPTISALQEKVLHDRACFERFVYSLSIPVT 91 GV+++YG+DFRNYA+ SLKRRI+ + E V ISALQE++LHDR C ERF+ SL++ VT Sbjct: 19 GVYQYYGHDFRNYAVSSLKRRIYSFMRLEGVDNISALQEQLLHDRTCLERFLLSLTVNVT 78 Query: 92 EMFRDPGLFLTFRQKVIPLLRTYPYIRIWHAGCSTGEEVYSMAILLHEEGLYDKARIYAT 151 MFRDP +L FR +V+P+LRTYP+IRIWHAGCS+GEEVYSMAILL EE LY + RIYAT Sbjct: 79 SMFRDPSFYLAFRNQVVPILRTYPFIRIWHAGCSSGEEVYSMAILLQEENLYHRCRIYAT 138 Query: 152 DMNARSLQQAKEGVYEISKMRQYTKNYMEAGGTRAFSEYYTAKYNSVILQPYLRKNIIFA 211 D N + LQ AK G++ + M++YT+ Y++AGG ++FSEYYTA Y+ I + LR+NIIFA Sbjct: 139 DTNEKVLQNAKSGIFPLKMMQEYTQLYLKAGGKQSFSEYYTAAYDHAIFRASLRENIIFA 198 Query: 212 EHNLATDTSFNEFNVIFCRNVMIYFNDELRDHVHGLFHESLSRFGVLVLGSKESIHFTRY 271 +HNLATD+SFNEFNVIFCRNV+IYFN L+ VH LFH SL FG+L LG +ESI FT Y Sbjct: 199 QHNLATDSSFNEFNVIFCRNVLIYFNQILQKRVHTLFHNSLCSFGILGLGKQESIRFTPY 258 Query: 272 SDSYEPLDRVEKIYRK 287 YE + + EK+YR+ Sbjct: 259 EQYYEEIAKGEKLYRR 274 >MA3542_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 531 Score = 132 bits (333), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 85/264 (32%), Positives = 142/264 (53%), Gaps = 25/264 (9%) Query: 38 GYDFRNYALPSLKRRIWHHVHAENVPTISA----LQEKVLHDRACFERFVYSLSIPVTEM 93 G+DF +Y ++ RR+ + N+ I L++ L A F F+ I VT Sbjct: 16 GHDFSHYKRGTISRRVERRMVVHNIGNIEEYVNYLEQNSLEVEALFRDFL----INVTSF 71 Query: 94 FRDPGLFLTFRQKVIPLL----RTYPYIRIWHAGCSTGEEVYSMAILLHEE-GLYD---K 145 FR+P F ++K+IP L IRIW GCSTGEE YS+ IL+ E L K Sbjct: 72 FRNPDAFDVLKEKIIPNLFIDKLVDESIRIWVPGCSTGEEAYSIGILIQEHMDLLKQPFK 131 Query: 146 ARIYATDMNARSLQQAKEGVYEISKMRQYTKNYMEAGGTRAFSEYYTAKYNSVILQPYLR 205 +I+ATD++A +++QA+ G Y S +K + R F++ Y +Q +R Sbjct: 132 VQIFATDIDAHAIEQARIGAYPASISVDVSKERL----GRFFTQGPDGSYR---VQKVIR 184 Query: 206 KNIIFAEHNLATDTSFNEFNVIFCRNVMIYFNDELRDHVHGLFHESLSRFGVLVLGSKES 265 +IF++H+L D F++ +++ CRNV+IY + EL+ + +FH +L+ L LG+ E+ Sbjct: 185 DMVIFSDHDLIKDPPFSKLDLVSCRNVLIYMDGELQKRLIPIFHYALNPGKFLFLGTSEN 244 Query: 266 IHFTRYSDSYEPLDRVEKIYRKNK 289 ++ +++ ++ LDR K+Y+ K Sbjct: 245 VY--GFTNLFDTLDRKAKLYKSRK 266 >MA1989_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 263 Score = 129 bits (324), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 84/260 (32%), Positives = 141/260 (54%), Gaps = 25/260 (9%) Query: 39 YDFRNYALPSLKRRIWHHVHAENVPTISA----LQEKVLHDRACFERFVYSLSIPVTEMF 94 +DF +Y ++ RR+ + N+ I L++K A F F+ I VT F Sbjct: 17 HDFSHYKKGTIVRRVERRMAVHNIKKIDEYVDYLEQKPAEVEALFRDFL----ISVTNFF 72 Query: 95 RDPGLFLTFRQKVIPLLRTYPY----IRIWHAGCSTGEEVYSMAILLHE--EGLYD--KA 146 R+P F F++K+IP L Y IR+W GCSTGEE YS+ ILL E E L Sbjct: 73 RNPKAFDAFQKKIIPNLFVGKYAGESIRVWVPGCSTGEEAYSIGILLQEHMEALKQTFNV 132 Query: 147 RIYATDMNARSLQQAKEGVYEISKMRQYTKNYMEAGGTRAFSEYYTAKYNSVILQPYLRK 206 +I+ATD++++++++A+ G Y S ++ +E T+ Y + N +R Sbjct: 133 QIFATDIDSQAIEEARIGTYPPSISVDVSEERLERFFTQGPDGNYRIRKN-------IRD 185 Query: 207 NIIFAEHNLATDTSFNEFNVIFCRNVMIYFNDELRDHVHGLFHESLSRFGVLVLGSKESI 266 +IF+EH+L D F++ +++ CRNV+IY + EL+ + LF+ +L+ L LGS E++ Sbjct: 186 MVIFSEHDLVKDPPFSKLDLLSCRNVLIYMDGELQKRLIPLFYYALNPGKFLFLGSSETV 245 Query: 267 HFTRYSDSYEPLDRVEKIYR 286 + + + ++ LDR K+Y+ Sbjct: 246 N--GFMNLFDTLDRKAKLYQ 263 >all1716_2 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 301 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 132/259 (50%), Gaps = 20/259 (7%) Query: 38 GYDFRNYALPSLKRRIWHHVHAENVPTISALQEKVLHDRACFERFVYSLSIPVTEMFRDP 97 G DF Y +LKRRI + + + + + +++A L I VT FRDP Sbjct: 16 GVDFTYYKQTTLKRRIQRRMMLYKLEKLEDYAQYLQNNQAEVLALYQDLLIKVTGFFRDP 75 Query: 98 GLFLTFRQKVIPLL---RTYPY-IRIWHAGCSTGEEVYSMAILLHEEGLYDKA-----RI 148 F + V P++ RT IRIW AGCSTGEE YSMAI L E L DK I Sbjct: 76 EAFEALKTNVFPIITKNRTPDSPIRIWVAGCSTGEEAYSMAICLLE-FLADKVANTPINI 134 Query: 149 YATDMNARSLQQAKEGVYEISKMRQYTKNYMEAGGTRAFSEYYTAKYNSVILQPYLRKNI 208 +ATD+N ++++A+ GVY+ S++ + ++ + Y +K +R+ Sbjct: 135 FATDINEVAIEKARIGVYKPSEVADVSPERLQRFFVQVEGGYQISKP--------VRELC 186 Query: 209 IFAEHNLATDTSFNEFNVIFCRNVMIYFNDELRDHVHGLFHESLSRFGVLVLGSKESIHF 268 IFA NL +D F+ ++I CRNV+IY ++ + +FH L G L+LG+ E++ Sbjct: 187 IFARQNLISDPPFSRLDLITCRNVLIYLGATVQKKLLPIFHYGLKSTGFLMLGTSETVG- 245 Query: 269 TRYSDSYEPLDRVEKIYRK 287 + D + +DR KIY + Sbjct: 246 -EFFDLFNLVDRKNKIYSR 263 >CAC2221 [NT] COG1352 Methylase of chemotaxis methyl-accepting proteins Length = 255 Score = 122 bits (307), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 72/236 (30%), Positives = 127/236 (53%), Gaps = 9/236 (3%) Query: 33 VHRFYGYDFRNYALPSLKRRIWHHVHAENVPTISALQEKVLHDRACFERFVYSLSIPVTE 92 V + +G D Y L RRI + +I + D +RF+ ++I VTE Sbjct: 10 VLKDFGLDLFAYKENQLHRRILSLISRVGASSIDEYVSMLKKDPEQRQRFLDFITINVTE 69 Query: 93 MFRDPGLFLTFRQKVIPLL-RTYPYIRIWHAGCSTGEEVYSMAILLHEEGLYDKARIYAT 151 FR+P +F +++ +L + ++IW A CS G E YS+AI+L + K I AT Sbjct: 70 FFRNPEIFREAEKEIKNMLYSSKEPLKIWSAACSIGAEPYSIAIILDKLDSLRKNNIIAT 129 Query: 152 DMNARSLQQAKEGVYEISKMRQYTKNYMEAGGTRAFSEYYTAKYNSVILQPYLRKNIIFA 211 D+++ L +AK G +YT+N ++ + +Y+ + I+ ++K I F Sbjct: 130 DIDSTILNKAKLG--------EYTENEIKNVEKQDLDKYFRIVGDKYIIVDKIKKMITFK 181 Query: 212 EHNLATDTSFNEFNVIFCRNVMIYFNDELRDHVHGLFHESLSRFGVLVLGSKESIH 267 +H+L D+ N F+++ CRNV+IYFN ++++ ++ F +SL + G+L +G+ ESI+ Sbjct: 182 KHDLILDSYDNNFDLVICRNVVIYFNQDVKEKIYKKFSQSLKKGGLLFVGATESIY 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,057,368 Number of extensions: 801496 Number of successful extensions: 2822 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 2765 Number of HSP's successfully gapped: 26 Length of query: 289 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 184 Effective length of database: 26,268,361 Effective search space: 4833378424 Effective search space used: 4833378424 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)