BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0012.p (127 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_rsbW [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protei... 94 6e-20 BH0528 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein... 88 3e-18 lin0893 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protei... 86 1e-17 BH1537 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein... 64 4e-11 BS_spoIIAB [T] COG2172 Anti-sigma regulatory factor (Ser/Thr pro... 56 1e-08 >BS_rsbW [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein kinase) Length = 160 Score = 93.6 bits (231), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 54/124 (43%), Positives = 80/124 (64%), Gaps = 6/124 (4%) Query: 1 LNLYGIASKMGFTYEDIEDMKVAVSEACNNSVLYAYGQE-DGLVDVIFEVGSSALSITVK 59 L L G+AS+MG+TY++IED+K+AVSEAC N+V +AY ++ +G V + F V L + V Sbjct: 24 LTLSGVASRMGYTYDEIEDLKIAVSEACTNAVQHAYKEDKNGEVSIRFGVFEDRLEVIVA 83 Query: 60 DEGESFDGMDASGERMTLHDKE--LNDVQVGGLGFYLMQALMDDVSVVSEAGKGTVVTLT 117 DEG+SFD D + + + ++ + GGLG YLM+ LMD+V V + + G V +T Sbjct: 84 DEGDSFD-FDQKQQDLGPYTPSHTVDQLSEGGLGLYLMETLMDEVRVQNHS--GVTVAMT 140 Query: 118 KRLN 121 K LN Sbjct: 141 KYLN 144 >BH0528 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein kinase) Length = 162 Score = 88.2 bits (217), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 53/126 (42%), Positives = 80/126 (63%), Gaps = 6/126 (4%) Query: 1 LNLYGIASKMGFTYEDIEDMKVAVSEACNNSVLYAYGQEDGLVDVIFEVGSSALSITVKD 60 L + GIA+++G+TY+DIED+K+AV+EAC N V +AY E G + + F + + I V D Sbjct: 25 LTVSGIANRLGYTYDDIEDIKIAVAEACTNVVDHAY-DEGGTMGLSFHLYEDRMEIIVTD 83 Query: 61 EGESFDGMDASGERMTLHD--KELNDVQVGGLGFYLMQALMDDVSVVSEAGKGTVVTLTK 118 +G+SFD MDA E + D K + D++ GGLG +L+ LMD V + ++ G V+ +TK Sbjct: 84 QGQSFD-MDAFRESLGPIDGSKPIQDLKEGGLGLFLINTLMDKVEMKEDS--GVVLAMTK 140 Query: 119 RLNFSE 124 L E Sbjct: 141 FLQRDE 146 >lin0893 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein kinase) Length = 157 Score = 86.3 bits (212), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 50/124 (40%), Positives = 81/124 (65%), Gaps = 6/124 (4%) Query: 1 LNLYGIASKMGFTYEDIEDMKVAVSEACNNSVLYAY-GQEDGLVDVIFEVGSSALSITVK 59 L+L GIAS+ GF+YE IED+K+AVSEA NSV +A+ G+EDG + V + + L + V Sbjct: 24 LSLSGIASRAGFSYEAIEDLKIAVSEAITNSVKHAFKGEEDGEITVEYHIYEDKLEVRVS 83 Query: 60 DEGESFDGMDASGERMTLHD--KELNDVQVGGLGFYLMQALMDDVSVVSEAGKGTVVTLT 117 D G SFD ++ + + ++ ++ +++GGLG +L++ LMDDV + + +G V +T Sbjct: 84 DNGTSFD-LETRKQEIGPYEVGEDAEMMRIGGLGLFLIETLMDDVKLYYD--EGVSVVMT 140 Query: 118 KRLN 121 K +N Sbjct: 141 KYIN 144 >BH1537 [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein kinase) Length = 146 Score = 64.3 bits (155), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 37/118 (31%), Positives = 66/118 (55%), Gaps = 4/118 (3%) Query: 8 SKMGFTYEDIEDMKVAVSEACNNSVLYAY-GQEDGLVDVIFEVGSSALSITVKDEGESFD 66 +++ T +++ ++K VSEA N++++ Y GQ DG+V + + +T++DEG Sbjct: 28 AQLDPTMDEMTEIKTVVSEAVTNAIIHGYQGQPDGVVYIHASIDQGVFELTIRDEGMGIS 87 Query: 67 GMDASGERMTLHDKELNDVQVGGLGFYLMQALMDDVSVVSEAGKGTVVTLTKRLNFSE 124 ++ + + + EL + G+GF +M+ MD+V VVSE GT V L K L S+ Sbjct: 88 DVEEARQPLYTTKPEL---ERSGMGFTIMENFMDEVKVVSEPMIGTTVFLKKHLTKSK 142 >BS_spoIIAB [T] COG2172 Anti-sigma regulatory factor (Ser/Thr protein kinase) Length = 146 Score = 55.8 bits (133), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 33/118 (27%), Positives = 66/118 (55%), Gaps = 4/118 (3%) Query: 8 SKMGFTYEDIEDMKVAVSEACNNSVLYAYGQE-DGLVDVIFEVGSSALSITVKDEGESFD 66 +++ T +++ ++K VSEA N++++ Y + +G V + + + +T++DEG Sbjct: 28 AQLDPTMDELTEIKTVVSEAVTNAIIHGYEENCEGKVYISVTLEDHVVYMTIRDEGLGIT 87 Query: 67 GMDASGERMTLHDKELNDVQVGGLGFYLMQALMDDVSVVSEAGKGTVVTLTKRLNFSE 124 ++ + + + EL + G+GF +M+ MDDVS+ S GT + LTK L+ S+ Sbjct: 88 DLEEARQPLFTTKPEL---ERSGMGFTIMENFMDDVSIDSSPEMGTTIRLTKHLSKSK 142 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,746,169 Number of extensions: 312216 Number of successful extensions: 1157 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1155 Number of HSP's successfully gapped: 5 Length of query: 127 Length of database: 38,378,431 Length adjustment: 93 Effective length of query: 34 Effective length of database: 27,652,369 Effective search space: 940180546 Effective search space used: 940180546 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 109 (46.6 bits)