BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0008.p (585 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2927 [G] COG0366 Glycosidases 566 e-161 lin2231 [G] COG0366 Glycosidases 503 e-142 BS_yvdF [G] COG0366 Glycosidases 472 e-133 L128694 [G] COG0366 Glycosidases 462 e-130 SPy1304 [G] COG0366 Glycosidases 390 e-108 >BH2927 [G] COG0366 Glycosidases Length = 578 Score = 566 bits (1459), Expect = e-161, Method: Compositional matrix adjust. Identities = 280/539 (51%), Positives = 367/539 (68%), Gaps = 9/539 (1%) Query: 8 SVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDRTH---SEYAMERLSS 64 ++YHR +YAYD++ +HIRLRT ++ V +V+LI GD Y W + S+ M S Sbjct: 4 TIYHRPKSEDAYAYDKSTMHIRLRTGKNHVRKVNLIYGDPYDWTKNQWVTSKKEMTPTGS 63 Query: 65 DNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFLEHPPEIIYEGLFDFPILSPED 124 D D+W V PP+RRL Y F + T+Y+ E GFL+ P + + F FP ++P D Sbjct: 64 DAQHDFWFVEVTPPYRRLRYGFEIITKTETIYYGESGFLDTPSKDTSD-YFCFPFMNPAD 122 Query: 125 VHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWG-GTPSPTNFFGGDLQGVLEHLDY 183 + PP WVKD V+YQIFPERFANGDPS +PEG L WG P+PTN FGGDLQG+++HLDY Sbjct: 123 LFSPPEWVKDTVWYQIFPERFANGDPSRNPEGTLPWGSAAPTPTNHFGGDLQGIIDHLDY 182 Query: 184 LCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLLDGV 243 L LGIN IY P+F A++NHKYDT DYL++DPHFG K LVD CH +GIR++LD V Sbjct: 183 LQELGINGIYLTPIFEASSNHKYDTIDYLKVDPHFGDKTTFKRLVDECHKRGIRIMLDAV 242 Query: 244 FNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPKFNTANP 303 FNH G FPPF DVL+N + S+Y+DWFH++++P V P YDTFAF P MPKFNT N Sbjct: 243 FNHAGFYFPPFQDVLKNQQHSKYSDWFHLRDFP-VRAEEPPNYDTFAFTPFMPKFNTENE 301 Query: 304 EVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGEIMHDSL 363 EV+EYLL V YWI+E +DGWRLDVANEV H FWR+FR VK + PD Y++GE+ H+SL Sbjct: 302 EVREYLLNVATYWIKEFDIDGWRLDVANEVDHAFWREFRKRVKALKPDLYILGEVWHNSL 361 Query: 364 PWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSFNLLDSH 423 PWL GDQFDAVMNY LTN +++F + + +F + L YP + +V+FNLL SH Sbjct: 362 PWLAGDQFDAVMNYPLTNAIIDFAGKQKIGQQRFEHVITQLTHMYPIHVQQVAFNLLGSH 421 Query: 424 DTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEWEPRKQN 483 DT R LT D+ R+KL FL L + +GTPCIYYGDEIGM G DP RKCM WE +QN Sbjct: 422 DTPRILTQTDNDVARVKLQFLLLFSQRGTPCIYYGDEIGMTGGQDPGCRKCMVWEEEEQN 481 Query: 484 QDLLEYYRWAIRLRKANSAFRSSA-IKFLEHPEHPELLVYERWDEHDRFLVIMNNSELP 541 Q+LL + + I+LRK AF + + F+E+ LL+Y++ E+ + L ++N+++ P Sbjct: 482 QELLSFVKRLIQLRKQEKAFGTEGDLTFVENDS--SLLIYQKTYENQQLLFVINSTDQP 538 >lin2231 [G] COG0366 Glycosidases Length = 591 Score = 503 bits (1295), Expect = e-142, Method: Compositional matrix adjust. Identities = 260/593 (43%), Positives = 354/593 (59%), Gaps = 26/593 (4%) Query: 9 VYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGW--DRTHSE-YAMERLSSD 65 +YH+ +++Y+YD +HIR+RTKR D+S+V LI D Y W ++ SE Y+M +++ Sbjct: 6 IYHQPASSYAYSYDAKTLHIRIRTKRLDISKVTLIAADPYLWKNEKWQSESYSMRKIAET 65 Query: 66 NLFDYWQAAVRPPHRRLAYYFAL-HQDGNTVYFLEKGFLEHPPEIIYEGL---FDFPILS 121 DYW A+ P HRRL Y F L +G T ++ +GF E P E + F FP + Sbjct: 66 EEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFE-PTEANLATMDYYFKFPFIH 124 Query: 122 PEDVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGT-PSPTNFFGGDLQGVLEH 180 D + P WV + ++YQIFPERFANG+P+ PE L WG P+ T+FFGGDL+G+++H Sbjct: 125 AVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDLEGIIQH 184 Query: 181 LDYLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIRVLL 240 LDYL LGIN +Y P+F A TNHKYDT DY +IDPHFG E ++LV H +GIR++L Sbjct: 185 LDYLAELGINGVYLTPVFEAPTNHKYDTIDYKKIDPHFGDKETFRKLVQEAHKRGIRIML 244 Query: 241 DGVFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNG-----GIPT--YDTFAFEP 293 D VFNH G T + DV+E S Y DWFH+ +P N G PT YDTFAF Sbjct: 245 DAVFNHIGDTSLEWQDVVEKEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTFAFTT 304 Query: 294 IMPKFNTANPEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAY 353 MPK NTANPEV++YLL + YWI E +DGWRLDVANEV H FW++F+ V+ D Y Sbjct: 305 HMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEKEDIY 364 Query: 354 LVGEIMHDSLPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQIT 413 ++GEI HDS WLLGD+F AVMNY T ++ F + + Q + YP Q+ Sbjct: 365 ILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQYMRYPNQVN 424 Query: 414 EVSFNLLDSHDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRK 473 EV FN+LDSHDT R LT D ++K A F+ + G+PCIYYG EIGMDG DP RK Sbjct: 425 EVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDGGNDPGCRK 484 Query: 474 CMEWEPRKQNQDLLEYYRWAIRLRKANSAFRSSA-IKFLEHPEHPELLVYERWDEHDRFL 532 CMEW+ KQNQD+L + + I LRK N A +S + +L + + + ++ Sbjct: 485 CMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELNWLGASSETGITAFSKELNGEKLH 544 Query: 533 VIMNNSELPIDAHISALKPES-TWINLELQQPADIGERGQLQLPAYGYVILRE 584 + N ++ D IS + W N + + L +PA G+++++E Sbjct: 545 FLFNQAKEDKDFTISFENTATDIWNNRAVTE--------NLVVPAKGFLVIKE 589 >BS_yvdF [G] COG0366 Glycosidases Length = 589 Score = 472 bits (1215), Expect = e-133, Method: Compositional matrix adjust. Identities = 234/508 (46%), Positives = 309/508 (60%), Gaps = 12/508 (2%) Query: 3 MIHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGWDR---THSEYAM 59 M+ +++H+ +Y+YD +HI++RTK+ D + I GD Y ++ + +E M Sbjct: 1 MMEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPM 60 Query: 60 ERLSSDNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKG---FLEHPPEIIYEGLFD 116 ++++ + DYW A V PP RRL Y F + D ++F G + E E I+ F Sbjct: 61 RKIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHY-YFK 119 Query: 117 FPILSPEDVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWGGT-PSPTNFFGGDLQ 175 FP + D Q P WVK V+YQIFPERFANG P+ L WG P +FFGGDLQ Sbjct: 120 FPFVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQ 179 Query: 176 GVLEHLDYLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKG 235 G+++ LDYL LG+N IY P+F+A +NHKYDT DY IDPHFG EL + LV H +G Sbjct: 180 GIVDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRG 239 Query: 236 IRVLLDGVFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIM 295 +R++LD VFNH G P + DV++NG SRY DWFH+ +P + YD FAF M Sbjct: 240 MRIMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDDN----YDRFAFTADM 295 Query: 296 PKFNTANPEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLV 355 PK NTANPEV++YLL + YWI E +DGWRLDVANEV H FW+ FR V PD Y++ Sbjct: 296 PKLNTANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYIL 355 Query: 356 GEIMHDSLPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEV 415 GEI H + PWL GD+F A MNY T ++ +FA SA + + V L N +Q EV Sbjct: 356 GEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEV 415 Query: 416 SFNLLDSHDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCM 475 FNLLDSHDT R LT C D ++ + F+ G+PCIYYG EIG++GE DP RKCM Sbjct: 416 MFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCM 475 Query: 476 EWEPRKQNQDLLEYYRWAIRLRKANSAF 503 WE KQNQD+L++ + I LRK + Sbjct: 476 VWEKEKQNQDMLQFMKRLIALRKQENTL 503 >L128694 [G] COG0366 Glycosidases Length = 584 Score = 462 bits (1190), Expect = e-130, Method: Compositional matrix adjust. Identities = 227/521 (43%), Positives = 313/521 (60%), Gaps = 21/521 (4%) Query: 8 SVYHRMGQNWSYAYDETVIHIRLRTKRDDVSRVDLICGDKYGW---DRTHSEY------A 58 ++YHR ++Y Y + ++H+RL+T++ DV +V+L GD Y W D ++Y Sbjct: 5 AIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVE 64 Query: 59 MERLSSDNLFDYWQAAVRPPHRRLAYYFALHQ-DGNTVYFLEKGFLEHPPEIIYE--GLF 115 M++ S N+ DY++A + +R+ Y F + DG V + ++G ++ ++ + G F Sbjct: 65 MKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGAF 124 Query: 116 DFPILSPEDVHQPPAWVKDAVFYQIFPERFANGDPSNDPEGVLEWG--GTPSPTNFFGGD 173 P D + P WVK+ V+YQIFPERFANGD SNDPEG W P +F+GGD Sbjct: 125 RLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDFYGGD 184 Query: 174 LQGVLEHLDYLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHA 233 LQGV++HL++L LG+N IYF P+F A +NHKYDT DY+ ID FG EL K LV HA Sbjct: 185 LQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVKEAHA 244 Query: 234 KGIRVLLDGVFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVV-------NGGIPTY 286 +GI+V+LD VFNH G T + DVL+N S++ADWFHV WP N TY Sbjct: 245 RGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATY 304 Query: 287 DTFAFEPIMPKFNTANPEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVK 346 DTFAF P MPK NTANPEV+ YLLKV YWI E +D WRLDVA+EV H FW+ FRT Sbjct: 305 DTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCD 364 Query: 347 QINPDAYLVGEIMHDSLPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLA 406 D Y++GE+ H + PWL+GD+F AVMNY T+ + + + S Q ++ T L Sbjct: 365 AAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNINTQLT 424 Query: 407 NYPQQITEVSFNLLDSHDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGE 466 Y +Q+ ++ FN+LDSHDT R LT+ + +KL F G PCIYYGDE + G Sbjct: 425 LYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGDEYAVTGG 484 Query: 467 YDPHNRKCMEWEPRKQNQDLLEYYRWAIRLRKANSAFRSSA 507 DP RK M WE + Q+ ++ ++++ I LRK S + Sbjct: 485 MDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDS 525 >SPy1304 [G] COG0366 Glycosidases Length = 567 Score = 390 bits (1001), Expect = e-108, Method: Compositional matrix adjust. Identities = 210/542 (38%), Positives = 307/542 (56%), Gaps = 15/542 (2%) Query: 1 VGMIHLESVYHRMGQNWSYAYDETVIHIRLRTKRDDVS-RVDLICGDKYGWDRTHSEYAM 59 GM+H+ + +A + + IRLR ++D V+L+ G KYG+ E M Sbjct: 4 AGMLHIPD------SRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEM 57 Query: 60 ERLSSDNLFDYWQAAVRPPHRRLAYYFALHQDGNTVYFLEKGFL-EHPPEIIYEGLFDFP 118 +D+ Y++ + RLAY F + ++G T YF E G + + F P Sbjct: 58 SVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMP 117 Query: 119 ILSPEDVHQPPAWVKDAVFYQIFPERFANGD-PSNDPEGVLEWGGTPSPTNFFGGDLQGV 177 ++ DVHQ W KDAVFYQIF +RF GD D + W P+P +F GGDL+G+ Sbjct: 118 YINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGWLEKPTPKSFAGGDLKGI 177 Query: 178 LEHLDYLCALGINAIYFNPLFAATTNHKYDTADYLRIDPHFGTNELLKELVDACHAKGIR 237 E LDYL LGI IY P+F + +NHKYD +DY IDP FGT L+EL+D H GI+ Sbjct: 178 TEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIK 237 Query: 238 VLLDGVFNHCGHTFPPFMDVLENGRASRYADWFHVKEWPPVVNGGIPTYDTFAFEPIMPK 297 ++LD VFNH F DVL G+ S++ DWF + P ++ + Y+TFA MPK Sbjct: 238 IILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSMD--LVNYETFAGCNYMPK 295 Query: 298 FNTANPEVKEYLLKVGRYWIEEIGVDGWRLDVANEVSHQFWRDFRTMVKQINPDAYLVGE 357 +NT+N +V++YL+++GRYWI+E +DGWRLDV++EVSH FWR FR VK DA L+GE Sbjct: 296 WNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGE 355 Query: 358 IMHDSLPWLLGDQFDAVMNYQLTNMLLNFFARGQTSAGQFSRSVGTLLANYPQQITEVSF 417 HD+ P+L GDQ+D +MNY T L++FA ++ Q + + +L Q+ ++ Sbjct: 356 NWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNL 415 Query: 418 NLLDSHDTVRFLTLCGGDIRRLKLAFLFLMTYQGTPCIYYGDEIGMDGEYDPHNRKCMEW 477 NLLDSHDT RF T G +L A L + G PC+YYG E+ M+G YDP +R+ W Sbjct: 416 NLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPW 475 Query: 478 EPRKQNQDLLEYYRWAIRLRKANSAFRSSAIKFLEHPEHPELLVYERWDEHDRFLVIMNN 537 + ++++D + I LR+ A + AI +++ E + L+ +R +++NN Sbjct: 476 DDAERDKDFFSKVKAIIALRQ-QPALQEGAIS-IDYDE--DCLILKRSLGEQSISLVLNN 531 Query: 538 SE 539 E Sbjct: 532 GE 533 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.141 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 48,944,441 Number of extensions: 2154133 Number of successful extensions: 4526 Number of sequences better than 1.0e-05: 113 Number of HSP's gapped: 4221 Number of HSP's successfully gapped: 116 Length of query: 585 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 473 Effective length of database: 25,461,023 Effective search space: 12043063879 Effective search space used: 12043063879 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)