BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0007.p (449 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvfK [G] COG2182 Maltose-binding periplasmic proteins/domains 207 2e-53 YPO0856 [G] COG2182 Maltose-binding periplasmic proteins/domains 197 4e-50 BH2019 [G] COG2182 Maltose-binding periplasmic proteins/domains 192 6e-49 SPy1306 [G] COG2182 Maltose-binding periplasmic proteins/domains 182 1e-45 BH2926 [G] COG2182 Maltose-binding periplasmic proteins/domains 181 3e-45 >BS_yvfK [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 421 Score = 207 bits (528), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 136/382 (35%), Positives = 200/382 (52%), Gaps = 12/382 (3%) Query: 77 KLMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPH 136 +L+VWE KE+ +K A FEK++ V V V E Q L DGPA DVLT+P Sbjct: 39 ELVVWEDKEKSNGIKDAVAAFEKEHDVKVKVVEKPYAKQIEDLRMDGPAGTGPDVLTMPG 98 Query: 137 DQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVP 196 DQI AV GLL V +++ ++ + ++Q+ D LYG PK+VET LFYNKDL+ Sbjct: 99 DQIGTAVTEGLLKELHV-KKDVQSLYTDASIQSQMVDQKLYGLPKAVETTVLFYNKDLIT 157 Query: 197 EA--PKTWNDIIAFANTYNKPKENKFAIMWEFVGYYTYPFIGSFGGYIFG---DSNSNPK 251 E PKT + ++ + F +++ + YY + +GGYIFG D + NP Sbjct: 158 EKELPKTLEEWYDYSKKTADGSKFGFLALFDQI-YYAESVMSGYGGYIFGKAKDGSYNPS 216 Query: 252 DIGLNNEGTIKGFTFLQSLKK--ILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWSTGSF 308 DIG+NNEG +KG +Q K + P G+ +V LF EGK A I GPW+ +F Sbjct: 217 DIGINNEGAVKGAALIQKFYKDGLFPAGIIGEQGINVLESLFTEGKAAAIISGPWNVEAF 276 Query: 309 KDH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYK 367 + +N+G+ LPK NG+ M SF GVKSY V++++ A+ A F +++ N Y+ Sbjct: 277 SNAGINYGITKLPKLENGKNMSSFIGVKSYNVSAFSKNEELAQELAVFLANEKNSKTRYE 336 Query: 368 MTGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAFVSLWNDP 427 T +PA I EQ + S P P I E+N +W P +A ++ Sbjct: 337 ETKEVPAVKSLANDPAIMKSEAARAVTEQSRFSEPTPNIPEMNEIWTPADSALQTVATGK 396 Query: 428 GLDVKQTMDQTVKTIQSDLESK 449 D KQ +DQ +T + +++K Sbjct: 397 A-DPKQALDQAAETAKGQIKAK 417 >YPO0856 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 420 Score = 197 bits (500), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 135/383 (35%), Positives = 201/383 (52%), Gaps = 18/383 (4%) Query: 77 KLMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPH 136 +L VWE ++ +K ADFEK++ V VNV E+ Q +L DGPA + DVL +P+ Sbjct: 39 QLTVWEDIKKSAGIKEAIADFEKQHQVKVNVLEMPYAQQIEKLRLDGPAGIGPDVLVIPN 98 Query: 137 DQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVP 196 DQ+ AV GLL P V + + + ++ A + D LYG PK+VET + YNKD++P Sbjct: 99 DQLGGAVVQGLLTPLSV-DPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLP 157 Query: 197 EAPKTWNDIIAFANTYNKPKENKFAIMWEFVG-YYTYPFIGSFGGYIFG-DSNSNPK--D 252 T ++ AF+ + ENK+ ++ +F YY++ I GGYIFG D+N + K D Sbjct: 158 TPLATLDEYAAFSK--KQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKAND 215 Query: 253 IGLNNEGTIKGFTFLQSL--KKILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWSTGSFK 309 IGLN G ++ T+L++ + P+ GD + LF E K A I+GPW+ ++ Sbjct: 216 IGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGLNAIDSLFTEKKAAAVINGPWAFQPYE 275 Query: 310 DH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYKM 368 +NFGV PLP PNG++M SF GVK Y V++++ A+ F F + Y++ Sbjct: 276 AAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYVKTRYQV 335 Query: 369 TGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAF---VSLWN 425 T IPA + IKND Q + MP I E+ VW P +A V+ Sbjct: 336 TKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQ 395 Query: 426 DPGLDVKQTMDQTVKTIQSDLES 448 +P K +D VK I +E+ Sbjct: 396 EP----KVALDNAVKQINMQIEA 414 >BH2019 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 424 Score = 192 bits (489), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 143/444 (32%), Positives = 214/444 (48%), Gaps = 37/444 (8%) Query: 14 KRSKMNAVSKIVVLCMLLVMTATACSSNSPNTAPSPKGGNNQTAETSAPENTADPEMEPE 73 KR + + + L + LV AC P +GG + TAE A Sbjct: 4 KRQRFIGIVSGIALSLALV----AC-------GPQDEGGES-TAEAPASN---------- 41 Query: 74 QGAKLMVWEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLT 133 +L++WE +E+ E ++ A FE ++GV V V E Q L DGPA DV T Sbjct: 42 ---ELLIWEDQEKSEGIEEAVAQFEAEHGVTVTVVEKPYAQQLEDLRLDGPAGTGPDVFT 98 Query: 134 LPHDQISQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKD 193 +P DQI AV GL V E+ ++ E A+ + DG +YG PK+VET L+YNK+ Sbjct: 99 MPGDQIGTAVTEGLE-KELVMGEDIQSLYTEAAMLSQIVDGKVYGLPKAVETTMLYYNKE 157 Query: 194 LVPE--APKTWNDIIAFANTYNKPKENKFAIMWEFVGYYTYPFIGSFGGYIFG---DSNS 248 L+ E P+T ++ + + + +++ + YY +G +GGY+FG N Sbjct: 158 LISEDDLPETLDEWFDLSKEFREDDMYGLLALFDQI-YYAQSVLGGYGGYVFGLDDQGNY 216 Query: 249 NPKDIGLNNEGTIKGFTFLQSL--KKILPLNA-GDITFDVKTQLFQEGKLAMNIDGPWST 305 +P DIGL+ EG I+G +Q + + P G+ +V LF EGK A I GPW+ Sbjct: 217 DPTDIGLDTEGAIEGAEQIQRFYGEGLFPSGIIGEQGINVLDSLFTEGKAAAVISGPWNL 276 Query: 306 GSF-KDHVNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLK 364 + + +N+GVV LP+ NG M SF GVKSY V+SY+ A F F ++++N Sbjct: 277 EPYARAGINYGVVKLPELDNGLNMSSFMGVKSYNVSSYSKNAELAEEFVKFIANEENSRT 336 Query: 365 NYKMTGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEPLKAAFVSLW 424 Y+ T +PA E + EQ Q S P I E+N VW P AA ++ Sbjct: 337 RYERTKEVPAVQVLAEDPVVTESDAAKAVAEQSQFSELTPNIPEMNEVWTPTDAALQTIA 396 Query: 425 NDPGLDVKQTMDQTVKTIQSDLES 448 + + Q V+TI+ +E+ Sbjct: 397 TGNA-SPRDALTQAVETIRGQIEA 419 >SPy1306 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 419 Score = 182 bits (462), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 127/409 (31%), Positives = 198/409 (48%), Gaps = 47/409 (11%) Query: 21 VSKIVVLCMLLVMTATACSSNSPNTAPSPKGGNNQTAETSAPENTADPEMEPEQGAKLMV 80 S + L + V+ TAC S GG ET G ++V Sbjct: 8 TSVLGTLSLASVLPLTACVS----------GGGKGVKET--------------DGKTIVV 43 Query: 81 WEAKEQLEYMKGVAADFEKKYGVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPHDQI- 139 + ++Y+K + +FEK++ V V V++ D +L+TDGP + DV P D++ Sbjct: 44 SVDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRVG 103 Query: 140 ---SQAVKAGLLLPNDVFEEETKASMVETAVQASSYDGVLYGYPKSVETYALFYNKDLVP 196 ++ A + L N ++T +V + DG YG P +ET +YNKDLVP Sbjct: 104 GLGTEGQIAEVTLGNSKEFDDTVKKLV-------TIDGKTYGAPDVIETLVTYYNKDLVP 156 Query: 197 EAPKTWNDI------IAFANTYNKPKENKFAIMW-EFVGYYTYPFIGSFGGYIFGDSNSN 249 +APK++ ++ FA K F W +F YY Y I +GGYIFGD + Sbjct: 157 QAPKSFTELEVLQKDSKFAFASEPGKSVGFLAKWTDF--YYGYGLIAGYGGYIFGDKGTK 214 Query: 250 PKDIGLNNEGTIKGFTFLQSLKKILPLNAGDI--TFDVKTQLFQEGKLAMNIDGPWSTGS 307 P D+GL N+GT++G + + P D D T+ F K + IDGPW+ S Sbjct: 215 PSDLGLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASS 274 Query: 308 FKDH-VNFGVVPLPKGPNGEEMISFSGVKSYYVNSYTAYPVAARLFAHFASSKDNQLKNY 366 FKD VNFGV+ +P NG++ F+G K++ +++Y+ A+ F + ++ +NQ + Y Sbjct: 275 FKDAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY 334 Query: 367 KMTGAIPASIEAGESQEIKNDPITLGFFEQFQHSVPMPAISEINAVWEP 415 T IPA++ A + + +T QF+ + PMP I E+ VWEP Sbjct: 335 DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEP 383 >BH2926 [G] COG2182 Maltose-binding periplasmic proteins/domains Length = 429 Score = 181 bits (458), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 130/411 (31%), Positives = 206/411 (50%), Gaps = 23/411 (5%) Query: 45 TAPSPKGGNNQTAETSAPENTADPE-MEPEQGAKLMVWEAKE--QLEYMKGVAADFEKKY 101 P GN +E+ A E PE+ L +W E QL + + +E++ Sbjct: 24 CGPDRDEGNGTASESGASAGEDGGEGTAPEKPDTLTMWVNDEDAQLSAYEQIVEKYEEET 83 Query: 102 GVPVNVEELAGGDQGGRLATDGPAKLAADVLTLPHDQISQAVKAGLLLPNDVFEEETKAS 161 G+ V + + DQ L+ D A D+ P+D++ GL ++ E+ + Sbjct: 84 GISVEITPFSMLDQLDALSLDAAAGGGPDLFFQPNDRMGDIYLQGLAAELELTPEQLEG- 142 Query: 162 MVETAVQASSYDGVLYGYPKSVETYALFYNKDLVPEAPKTWNDIIAFANTYNKPKENKFA 221 E A+ A +Y+G G P +ETYALFYN +LVPEAP+T+ ++ A +++ Sbjct: 143 YPEGAIDALNYEGAQLGIPAVIETYALFYNTELVPEAPETFEEMEQIAAELTNASADEYG 202 Query: 222 IMWEFVG-YYTYPFIGSFGGYIFGDSNSNPKD---IGLNNEGTIKGFTFLQSL--KKILP 275 + E YY YPF+ + GGY+F D IGL NEG I+G + +QS + LP Sbjct: 203 FLMEATNFYYLYPFLEAHGGYVFEQDAEGVYDSASIGLANEGAIEGASLIQSWFEEGYLP 262 Query: 276 LNAGDITFDVKTQLFQEGKLAMNIDGPWSTGSFKDHV--NFGVVPLPKGPNGEEMISFSG 333 + I D+ + F++G++ I GPWS F + + N V PLPK NG+ SFSG Sbjct: 263 VG---INGDIMSGYFRDGQVGAVITGPWSIPEFSEALGDNLAVAPLPK-INGKNPASFSG 318 Query: 334 VKSYYVNSYTAYPVAARLFAHFASSKDNQLKNYKMTGAIPASIEAGESQEIKNDPITLGF 393 VK + VN Y+A A A F ++ +N L +++ G +PA + E++ PI Sbjct: 319 VKGWLVNEYSANQYWATDLALFITNPENSLTYFEVAGELPARTDVEIDDELRQ-PI---- 373 Query: 394 FEQFQHSVPMPAISEINAVWEPLKAA--FVSLWNDPGLDVKQTMDQTVKTI 442 EQ +++VPMP I +++VWEP++ A F+S DP +++ + Q I Sbjct: 374 LEQSEYAVPMPNIPAMSSVWEPMEDAFEFISQGEDPAEVLEEAVQQIADKI 424 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,812,839 Number of extensions: 1462317 Number of successful extensions: 2985 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 2935 Number of HSP's successfully gapped: 32 Length of query: 449 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 339 Effective length of database: 25,691,691 Effective search space: 8709483249 Effective search space used: 8709483249 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)