BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0001.p (261 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0298 [R] COG0596 Predicted hydrolases or acyltransferases (al... 106 4e-23 PA0231 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 105 8e-23 lin1782 [R] COG0596 Predicted hydrolases or acyltransferases (al... 104 2e-22 SSO0102 [R] COG0596 Predicted hydrolases or acyltransferases (al... 103 3e-22 DR0791 [R] COG0596 Predicted hydrolases or acyltransferases (alp... 99 7e-21 >RSc0298 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 279 Score = 106 bits (264), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 72/247 (29%), Positives = 116/247 (46%), Gaps = 9/247 (3%) Query: 15 DQGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDAPLGAYSIDQMADD 74 D G I+L H W+ + L+ +RV+ D RGHG + AP G Y++ QMADD Sbjct: 24 DGADGPWIMLAHALGVDHQMWDSIAHRLASRHRVLRYDARGHGGTTAPHGTYTLFQMADD 83 Query: 75 VLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGY-PDSGEARENRLK 133 LLDAL IP+ + +G S+GG + RH RL L + + P + A + Sbjct: 84 AAGLLDALSIPQVHFVGLSMGGMVAQILGVRHPQRLLSLTLCDTVCHTPVAAHAMWD--D 141 Query: 134 SVSMIQNEGIFAFVD-GLVPGLFAP--GAAPQLLERTKEIGYRTPPQGAVGAAMAMRERP 190 + ++ G+ V+ L L P A P++ R + + TPP G VGA +A++ Sbjct: 142 RIGQVEAHGMAGIVEPTLQRWLTTPFREAHPEVAARIRALLLATPPHGYVGACLAIKAL- 200 Query: 191 DRRDVISATALPVLLVAGAEDSKVTPE--QTFTSNKPNVTQATISGVGHMSMYEAPERLA 248 D RD + A P L+V G +D+ P+ + + PN + H++ E E Sbjct: 201 DTRDALPRIACPALVVVGEDDAGAPPDVARAIAGSLPNARLKVMPHAAHLAPIEQEEAFL 260 Query: 249 EIIKDYV 255 + +++ Sbjct: 261 TDLDEFL 267 >PA0231 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 263 Score = 105 bits (261), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 80/264 (30%), Positives = 121/264 (45%), Gaps = 7/264 (2%) Query: 1 MEIVRCEGSNICYSDQG-TGDVIVLLHGFCGSS-SYWEQVIPLLSGNYRVIAPDLRGHGV 58 M VR ++ YS +G G ++LL G+ W+ IP L+ ++RV+ D RGHG Sbjct: 1 MPTVRLADGDLNYSLEGPAGAPVLLLSNSLGTDLGMWDTQIPALTAHFRVLRYDTRGHGA 60 Query: 59 SDAPLGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHS 118 S G Y+I Q+ DVL+LLDALE+P + G S+GG I RL L ++ Sbjct: 61 SLVTPGPYAIGQLGADVLALLDALELPRVHFCGLSMGGLIGQWLGIHAGERLGRLVLCNT 120 Query: 119 TGYPDSGEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPGAA---PQLLERTKEIGYRTP 175 S E R+ +V + + D V F G A P +ER + T Sbjct: 121 AAKIASDEVWNTRIDTVLKGGEQAMRDLRDASVARWFTAGFAEREPAQVERIVAMLAATS 180 Query: 176 PQGAVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQT-FTSNKPNVTQATISG 234 PQG A+R+ D R+ + P L+VAG+ D+ TP+ F + Q Sbjct: 181 PQGYAANCAAVRD-ADFREQLGLVQAPTLIVAGSHDAVTTPDNARFMQARIADAQLVEFA 239 Query: 235 VGHMSMYEAPERLAEIIKDYVHAA 258 H+S EA + + + D++ +A Sbjct: 240 AAHLSNVEAGDAFSRRLVDFLLSA 263 >lin1782 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 301 Score = 104 bits (259), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 19/244 (7%), Positives = 42/244 (17%), Gaps = 20/244 (8%) Query: 17 GTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDAP--LGAYSIDQMADD 74 L + +R++ G + G + ++ A + Sbjct: 16 RPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAIN 75 Query: 75 VLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGYPDSGEARENRLKS 134 L A L + + G + + + + Sbjct: 76 RLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADL 135 Query: 135 VSMIQN-------EGIFAFVDGLVPGLFAPGAA-PQLLERTKEIGYRTPPQGAVGAAMAM 186 + +Q I GL A P+ + Sbjct: 136 LPHLQFLASQMNFLDIVPISAET--GLNVDTIAAIVRKH--LPEATHHFPEDYITDRSQR 191 Query: 187 R-----ERPDRRDVISATALPVLLVAGAEDSKVTP-EQTFTSNKPNVTQATISGVGHMSM 240 R + A + V + V Sbjct: 192 FMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKG 251 Query: 241 YEAP 244 + Sbjct: 252 AKIK 255 >SSO0102 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 301 Score = 103 bits (257), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 11/243 (4%), Positives = 33/243 (13%), Gaps = 22/243 (9%) Query: 23 VLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSDAPLGAYSIDQMADDVLSLLDAL 82 + + + + S + Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY 60 Query: 83 EIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGYPDSGEARENRLKSVSMIQNEG 142 + ++ A + + L+ + + V EG Sbjct: 61 VDTPGLHMEEKRAINRLMNKAASSS--IGDVELVIFVVEGTRWTPDD---EMVLNKLREG 115 Query: 143 IFA----------FVDGLVPGLFAPGAAPQLLERTKEIGYRTPPQGAVGAAMAMRERPDR 192 + A Q+ Sbjct: 116 KAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTI-------AAI 168 Query: 193 RDVISATALPVLLVAGAEDSKVTPEQTFTSNKPNVTQATISGVGHMSMYEAPERLAEIIK 252 A D + + + +++ E Sbjct: 169 VRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGG 228 Query: 253 DYV 255 + Sbjct: 229 YDI 231 >DR0791 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 305 Score = 99.0 bits (245), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 75/260 (28%), Positives = 123/260 (47%), Gaps = 10/260 (3%) Query: 4 VRCEGSNICYSDQGTGDVIVLLHGFCGSSSYWEQVIPLLSGNYRVIAPDLRGHGVSD-AP 62 V G+ + Y G+G ++L+HG+ + ++ ++SG YRVI DL G G S AP Sbjct: 46 VTVNGAQVYYKATGSGQPLLLIHGYPLNGELFKNN-RMMSG-YRVITVDLPGFGKSKLAP 103 Query: 63 LGAYSIDQMADDVLSLLDALEIPECYLLGHSLGGYITLSFAQRHASRLKGFGLIHSTGYP 122 SI+ A ++ +DA+++ + + G S+GG L + R KG LI +T P Sbjct: 104 GQTVSIENYAQTMVGFMDAMKLDKAVVGGMSMGGMTLLQMYKIAPERFKGLILIDTTAEP 163 Query: 123 DS-GEARENRLKSVSMIQNEGIFAFVDGLVPGLFAPGAAPQLLERTKEIG---YRTPPQG 178 EA R + Q +G+ + VD L+P + + ++ + +G + +G Sbjct: 164 AGIAEAAMWR-GTAQQAQQKGVASLVDLLMPRMLTGESRMKMPNQVAHLGNLIKQASVEG 222 Query: 179 AVGAAMAMRERPDRRDVISATALPVLLVAGAEDSKVTPEQTFTSNKP--NVTQATISGVG 236 AVGA A+ RPD V+ +P L+VAG ED+ PE NK I G G Sbjct: 223 AVGAGNALASRPDANPVLPTIKVPTLIVAGLEDNVTAPELQMKMNKAIRGSKLVLIPGAG 282 Query: 237 HMSMYEAPERLAEIIKDYVH 256 H S +E + + +++ Sbjct: 283 HASTFEKAGAMNAAVANWLR 302 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,712,184 Number of extensions: 867945 Number of successful extensions: 1973 Number of sequences better than 1.0e-05: 90 Number of HSP's gapped: 1957 Number of HSP's successfully gapped: 90 Length of query: 261 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 157 Effective length of database: 26,383,695 Effective search space: 4142240115 Effective search space used: 4142240115 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)