BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0021.p (1122 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1193 [G] COG3250 Beta-galactosidase/beta-glucuronidase 125 5e-28 TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase 110 1e-23 STM1062 [R] COG0488 ATPase components of ABC transporters with d... 110 1e-23 lacZ [G] COG3250 Beta-galactosidase/beta-glucuronidase 108 6e-23 ZlacZ [G] COG3250 Beta-galactosidase/beta-glucuronidase 108 6e-23 >TM1193 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1087 Score = 125 bits (313), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 130/486 (26%), Positives = 212/486 (43%), Gaps = 75/486 (15%) Query: 1 LNGEWDFMPLYGQPLNRTLPERLVYEA------RKVQVPSSWR-HGYPQPSGKKFGEVAE 53 LNG W F+ P +PE E +++VPS+W GY GK Sbjct: 45 LNGNWRFL-FAKNPFE--VPEDFFSEKFDDSNWDEIEVPSNWEMKGY----GKPIYTNVV 97 Query: 54 HGFAPLDVYGYPREWDAAEAGVLHRSFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAV 113 + F P + P+ D GV R ++PE + I L F+G+ ++++ ++I Sbjct: 98 YPFEPNPPF-VPK--DDNPTGVYRRWIEIPEDWFKKEIFLHFEGVRSFFYLWVNGKKIGF 154 Query: 114 WEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARGIWQ 173 +D P ++ ++PG+ V D + L D WF GI++ Sbjct: 155 SKDSCTPAEFRLTDVLRPGKNLITVEVLKWSDGSYLEDQDM-------WWFA----GIYR 203 Query: 174 DVFLESYPLVTLEDITIRTLFRQGLLEVDALIGTESGSAYAEPLRVFLKVNDRKPGALPV 233 DV+L + P + D+ +RT D +G ++FL V R G Sbjct: 204 DVYLYALPKFHIRDVFVRT---------DLDENYRNG-------KIFLDVEMRNLGE--- 244 Query: 234 LEAEAGAEAVPLQA-GEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYELELELR 292 E E E + G+ + L + E + F D + WS ETP+LY L+L+L Sbjct: 245 -EEEKDLEVTLITPDGDEKTLVKETVKPEDRVLSFAFDVKDPKKWSAETPHLYVLKLKL- 302 Query: 293 AGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWHF----QGAAQQTEGYIRNWY 348 GE D + FGFR+ + + NG P+ ++G + H +G A E I++ Sbjct: 303 --GE--DEKKVNFGFRKIEIKDGTLLFNGKPLYIKGVNRHEFDPDRGHAVTVERMIQD-I 357 Query: 349 RMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYGSSKSMDANHPDFIANC--- 405 ++ +Q +N++R P + + + D G+ ++DE I S +D + +AN Sbjct: 358 KLMKQHNINTVRTSHYPNQTKWYDLCDYFGLYVIDEANI--ESHGIDWDPEVTLANRWEW 415 Query: 406 -RAH---VQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRPIM 461 +AH ++R+V+RDKNHPS+I WS+ NE DG L ++ D+TR I Sbjct: 416 EKAHFDRIKRMVERDKNHPSIIFWSLGNE-----AGDGVNFEKAALW--IKKRDNTRLIH 468 Query: 462 VEGDNR 467 EG R Sbjct: 469 YEGTTR 474 >TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 563 Score = 110 bits (276), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 100/400 (25%), Positives = 174/400 (43%), Gaps = 57/400 (14%) Query: 79 SFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAVWEDGYLPLRLDISSRVKPGEEQHLH 138 +F VP+ + + I L F + V+L+ E++ YLP +D++ +VK GE + Sbjct: 55 TFYVPKELSQKHIRLYFAAVNTDCEVFLNGEKVGENHIEYLPFEVDVTGKVKSGENELRV 114 Query: 139 VV-----CGSFDQTVLPSGDRKITGLAGSWFGRMARGIWQDVFLESYPLVTLEDITIRTL 193 VV G F V SG + F S+P + + Sbjct: 115 VVENRLKVGGFPSKVPDSGTHTVG------------------FFGSFPPANFDFFPYGGI 156 Query: 194 FRQGLLEVDALIGTESGSAYAEPLRVFLKVNDRKP----GALPV---LEAEAGAEAVPLQ 246 R L+E + +A L +++ ++ +P G + V + EA + + ++ Sbjct: 157 IRPVLIEF---------TDHARILDIWVDTSESEPEKKLGKVKVKIEVSEEAVGQEMTIK 207 Query: 247 AGEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYELELELRAGGETLDRRTGTFG 306 GE + ++ + + F+L +AR WS E P LY L++EL TLD G Sbjct: 208 LGEEEKKIRTSNR--FVEGEFILE--NARFWSLEDPYLYPLKVELEKDEYTLD-----IG 258 Query: 307 FREFWCEGPQFMLNGTPVNLRGDSWHFQ----GAAQQTEGYIRNWYRMCRQAGVNSIRLH 362 R + + LNG PV L+G H + G I++ + + + NS R Sbjct: 259 IRTISWDEKRLYLNGKPVFLKGFGKHEEFPVLGQGTFYPLMIKD-FNLLKWINANSFRTS 317 Query: 363 AEPYPSDYLRIADEEGMLIVDETAIYGSSKSMDANHPDFIANCRAHVQRLVQRDKNHPSV 422 PY ++L +AD G+L++DE G ++ +P+ +++R++ R KNHPSV Sbjct: 318 HYPYSEEWLDLADRLGILVIDEAPHVGITRY--HYNPETQKIAEDNIRRMIDRHKNHPSV 375 Query: 423 ILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRPIMV 462 I+WSV NE + L + ++D TRP+++ Sbjct: 376 IMWSVANEPE--SNHPDAEGFFKALYETANEMDRTRPVVM 413 >STM1062 [R] COG0488 ATPase components of ABC transporters with duplicated ATPase domains Length = 635 Score = 110 bits (276), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 26/400 (6%), Positives = 72/400 (18%), Gaps = 57/400 (14%) Query: 79 SFQVPETMLGQRIVLRFDGIMQKAAVYLDRERIAVWEDGYLPLRLDISSRVKPGEEQHLH 138 IV R + + +A + + Sbjct: 55 LDDGRIIYEQDLIVARLQQDPPRNIAGSVYDFVAEGIEEQAEYLKRYHEISRLVMTDPSE 114 Query: 139 VV-----CGSFDQTVLPSGDRKITGLAGSWFGRMARGIWQDVFLESYPLVTLEDITIRTL 193 + E + L+ + Sbjct: 115 KNLNEMARVQEQLDHHNLWQLENR------------------INEVLAQLGLDPNAALSS 156 Query: 194 FRQGLLEVDALIGTESGSAYAEPLRVFLKVNDRKP----GALPV---LEAEAGAEAVPLQ 246 G L +A L +V + LE + Sbjct: 157 LSGGWLRK---------AALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTII 207 Query: 247 AGEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYELELELRAGGETLDRRTGTFG 306 + + + + E E + Sbjct: 208 FISHDRSFIRNMA--TRIVDLDRG--KLVTYPGNYDQYLLEKEEALRVEELQN-----AE 258 Query: 307 FREFWCEGPQFMLNGTPVNLRGDSWHFQ----GAAQQTEGYIRNWYRMCRQAGVNSIRLH 362 F + ++ G + + +++E Q + Sbjct: 259 FDRKLAQEEVWIRQGIKARRTRNEGRVRALKAMRRERSERREVM-GTAKMQVEEATRSGK 317 Query: 363 AEPYPSDYLRIADEEGMLIVDETAIYGSSKSMDANHPDFIANCRAHVQRLVQRDKNHPSV 422 + + + ++ + K A ++ ++ + + Sbjct: 318 IVFEMENVDYQVEGKQLVKDFSAQVQRGDKI--ALIGPNGCGKTTLLKLMLGQLQADSGR 375 Query: 423 ILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRPIMV 462 I + E+ + P + + + +MV Sbjct: 376 IHVGTKLEVA--YFDQHRAELDPEKTVMDNLAEGKQEVMV 413 >lacZ [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1024 Score = 108 bits (269), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 127/485 (26%), Positives = 204/485 (42%), Gaps = 71/485 (14%) Query: 1 LNGEWDFMPLYGQPLNRTLPERLVY----EARKVQVPSSWR-HGYPQP--SGKKFGEVAE 53 LNGEW F P +PE + EA V VPS+W+ HGY P + + Sbjct: 55 LNGEWRFAWF---PAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVN 111 Query: 54 HGFAPLDVYGYPREWDAAEAGVLHRSFQVPETML--GQRIVLRFDGIMQKAAVYLDRERI 111 F P + G +F V E+ L GQ ++ FDG+ ++ + + Sbjct: 112 PPFVPTE----------NPTGCYSLTFNVDESWLQEGQTRII-FDGVNSAFHLWCNGRWV 160 Query: 112 AVWEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARGI 171 +D LP D+S+ ++ GE + +V D + L D RM+ GI Sbjct: 161 GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQD----------MWRMS-GI 209 Query: 172 WQDVFLESYPLVTLEDITIRTLFR----QGLLEVDALIGTESGSAYAEPLRVFLKVNDRK 227 ++DV L P + D + T F + +LE + + E LR +L+V Sbjct: 210 FRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGE--------LRDYLRVT--- 258 Query: 228 PGALPVLEAEAGAEAVPLQAGEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYEL 287 +L E + + P + AD + L+ + +LWS E PNLY Sbjct: 259 -VSLWQGETQVASGTAPFGGEIIDERGGYAD-----RVTLRLNVENPKLWSAEIPNLYRA 312 Query: 288 ELELR-AGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWH----FQGAAQQTEG 342 +EL A G ++ GFRE E +LNG P+ +RG + H G + Sbjct: 313 VVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQT 372 Query: 343 YIRNWYRMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYGSSK---SMDANHP 399 +++ M +Q N++R P + + D G+ +VDE I + + P Sbjct: 373 MVQDILLM-KQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDP 431 Query: 400 DFIANCRAHVQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRP 459 ++ V R+VQRD+NHPSVI+WS+ NE G+ + L + ++ +D +RP Sbjct: 432 RWLPAMSERVTRMVQRDRNHPSVIIWSLGNE-------SGHGANHDALYRWIKSVDPSRP 484 Query: 460 IMVEG 464 + EG Sbjct: 485 VQYEG 489 >ZlacZ [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1024 Score = 108 bits (269), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 125/485 (25%), Positives = 204/485 (42%), Gaps = 71/485 (14%) Query: 1 LNGEWDFMPLYGQPLNRTLPERLVY----EARKVQVPSSWR-HGYPQP--SGKKFGEVAE 53 LNGEW F+ P +PE + +A V VPS+W+ HGY P + + Sbjct: 55 LNGEWQFVWF---PAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYTNVTYPITVN 111 Query: 54 HGFAPLDVYGYPREWDAAEAGVLHRSFQVPETML--GQRIVLRFDGIMQKAAVYLDRERI 111 F P + G +F V E+ L GQ ++ FDG+ ++ + + Sbjct: 112 PPFVPTE----------NPTGCYSLTFNVDESWLQEGQTRII-FDGVNSAFHLWCNGRWV 160 Query: 112 AVWEDGYLPLRLDISSRVKPGEEQHLHVVCGSFDQTVLPSGDRKITGLAGSWFGRMARGI 171 +D L D+S+ ++ GE + +V D + L D RM+ GI Sbjct: 161 GYGQDSRLLSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQD----------MWRMS-GI 209 Query: 172 WQDVFLESYPLVTLEDITIRTLFR----QGLLEVDALIGTESGSAYAEPLRVFLKVNDRK 227 ++DV L P + D + TLF + +LE + + E LR L+V Sbjct: 210 FRDVSLLHKPTTQISDFHVATLFNDDFSRAVLEAEVQMYGE--------LRDELRVT--- 258 Query: 228 PGALPVLEAEAGAEAVPLQAGEGRQLCENADNREWQKARFVLSWPDARLWSPETPNLYEL 287 +L E + + P + AD + L+ + +LWS E PN+Y Sbjct: 259 -VSLWQGETQVASGTAPFGGEIIDERGGYAD-----RVTLGLNVENPKLWSAEIPNIYRA 312 Query: 288 ELELR-AGGETLDRRTGTFGFREFWCEGPQFMLNGTPVNLRGDSWH----FQGAAQQTEG 342 +EL A G ++ GFRE E +LNG P+ +RG + H G + Sbjct: 313 VVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQT 372 Query: 343 YIRNWYRMCRQAGVNSIRLHAEPYPSDYLRIADEEGMLIVDETAIYGSSK---SMDANHP 399 +++ M +Q N++R P + + D G+ +VDE I + + P Sbjct: 373 MVQDILLM-KQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDP 431 Query: 400 DFIANCRAHVQRLVQRDKNHPSVILWSVQNEMRWVDGRDGYKQHIPGLMKLMRDLDSTRP 459 ++ V R+VQRD+NHPSVI+WS+ NE G+ + L + ++ +D +RP Sbjct: 432 RWLPAMSERVTRMVQRDRNHPSVIIWSLGNE-------SGHGANHDALYRWIKSVDPSRP 484 Query: 460 IMVEG 464 + EG Sbjct: 485 VQYEG 489 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 86,632,108 Number of extensions: 3943147 Number of successful extensions: 8610 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 8570 Number of HSP's successfully gapped: 20 Length of query: 1122 Length of database: 38,378,431 Length adjustment: 117 Effective length of query: 1005 Effective length of database: 24,884,353 Effective search space: 25008774765 Effective search space used: 25008774765 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 121 (51.2 bits)