BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0017.p (313 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl3030 [O] COG0492 Thioredoxin reductase 330 2e-90 RC0618 [O] COG0492 Thioredoxin reductase 320 1e-87 YDR353w [O] COG0492 Thioredoxin reductase 319 3e-87 all0737_1 [O] COG0492 Thioredoxin reductase 318 6e-87 RP445 [O] COG0492 Thioredoxin reductase 318 6e-87 >Cgl3030 [O] COG0492 Thioredoxin reductase Length = 346 Score = 330 bits (846), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 17/306 (5%), Positives = 45/306 (14%), Gaps = 1/306 (0%) Query: 1 MYKSIIIGTGPAGLTAAIYLARANLNPLVIEGPQPGGQLXXXXEVENFPGFPDGIMGPEL 60 ++ +GT G + A A L+ V +G Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 61 MDNMRKQAERFGAKFVTGWVNSVELGERPFKLNVEGMGALTTDTLILSTGATAKYLGIPG 120 E+ + + + YL + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQAL-DRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 121 EQDNIGRGVSTCATCDGFFFRGKEIVVVGGGDSALEEAGFLTRFASKVTLVHRREELRAS 180 + + ++ D+ E V+ +R Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 181 KIMQDRVRDNEKVAWSLNRTPLEVTSGDRGVNGIKVLNNVTGEEEFIEASGVFVAIGHHP 240 + + + + + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 241 NTAFLGGQITTDANGYIVTNPGTSETNIPGVFACGDVQDTRYKQAITAAGSGCMAAMDAE 300 + + G + + A Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 301 KYIESL 306 + Sbjct: 311 TMDQLK 316 >RC0618 [O] COG0492 Thioredoxin reductase Length = 346 Score = 320 bits (821), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 16/303 (5%), Positives = 49/303 (16%), Gaps = 2/303 (0%) Query: 3 KSIIIGTGPAGLTAAIYLARANLNPLVIEGPQPGGQLXXXXEVENFPGFPDGIMGPELMD 62 + N + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 63 NMRKQAERFGAKFVTGWVNSVELGERPFKLNVEGMGALTTDTLILSTGATAKYLGIPGEQ 122 + + ++ + D + + + Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 123 DNIGRGVSTCATCDGFFFRGKEIVVVGGGDSALEEAGFLTRFASKVTLVHRREELRASKI 182 ++ + + F G S L+ L + + + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 183 MQDRVRDNEKVAWSLNRTPLEVTSGD--RGVNGIKVLNNVTGEEEFIEASGVFVAIGHHP 240 + + +K T S G+ ++ G E + F + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 241 NTAFLGGQITTDANGYIVTNPGTSETNIPGVFACGDVQDTRYKQAITAAGSGCMAAMDAE 300 G + + + A + + Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 301 KYI 303 Sbjct: 306 LLG 308 >YDR353w [O] COG0492 Thioredoxin reductase Length = 346 Score = 319 bits (818), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 22/315 (6%), Positives = 49/315 (15%), Gaps = 10/315 (3%) Query: 3 KSIIIGTGPAGLTAAIYLARANLNPLVIEGPQP-----GGQLXXXXEVENFPGFPDGIMG 57 + + L + G L E+ P P+ Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 58 PELMDNMRKQAERFGAKFVTGWVNSVELGERPFKLNVE---GMGALTTDTLILSTGATAK 114 E K + S + + + Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 115 YLGIPGEQDNIGRGVSTCATCDGF--FFRGKEIVVVGGGDSALEEAGFLTRFASKVTLVH 172 YL + + ++ + + AG + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 173 RREELRASKIMQDRVRDNEKVAWSLNRTPLEVTSGDRGVNGIKVLNNVTGEEEFIEASGV 232 D R V F +G Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 233 FVAIGHHPNTAFLGGQITTDANGYIVTNPGTSETNIPGVFACGDVQDTRYKQAITAAGSG 292 ++ T +G + +I Q + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAS 304 Query: 293 CMAAMDAEKYIESLE 307 + +++ Sbjct: 305 TLLGATTMDQLKTNI 319 >all0737_1 [O] COG0492 Thioredoxin reductase Length = 346 Score = 318 bits (816), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 24/307 (7%), Positives = 46/307 (14%), Gaps = 8/307 (2%) Query: 5 IIIGTGPAGLTAAIYLARANLNPLVIEGPQ----PGGQLXXXXEVENFPGFPDGIMGPEL 60 L + P + E G Sbjct: 37 DYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNND 96 Query: 61 MDNMRKQAERFGAKFVTGWVNSVELGERPFKLNVEGMGALTTDTLILSTGATAKYLGIPG 120 QA + L L G + Sbjct: 97 KGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHW-PQRPTNCFGKLGYSWTDSAPAV 155 Query: 121 EQDNIGRGVSTCATCDGF--FFRGKEIVVVGGGDSALEEAGFLTRFASKVTLVHRREELR 178 + ++ E L + L R + Sbjct: 156 SLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSF 215 Query: 179 ASKIMQDRVRDNEKVAWSLNRTPLEVTSGDRGVNGIKVLNNVTGEEEFIEASGVFVAIGH 238 + + + ++ +T G T F SG Sbjct: 216 EVGLAEVSQYEGVELLAYSCLGFGTLT-GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 Query: 239 HPNTAFLGGQITTDANGYIVTNPGTSETNIPGVFACGDVQDTRYKQAITAAGSGCMAAMD 298 A + + A Q +++ S + A Sbjct: 275 AAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI 334 Query: 299 AEKYIES 305 + Sbjct: 335 EAVHQVY 341 >RP445 [O] COG0492 Thioredoxin reductase Length = 346 Score = 318 bits (815), Expect = 6e-87, Method: Compositional matrix adjust. Identities = 16/303 (5%), Positives = 49/303 (16%), Gaps = 2/303 (0%) Query: 3 KSIIIGTGPAGLTAAIYLARANLNPLVIEGPQPGGQLXXXXEVENFPGFPDGIMGPELMD 62 + N + + Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 63 NMRKQAERFGAKFVTGWVNSVELGERPFKLNVEGMGALTTDTLILSTGATAKYLGIPGEQ 122 + + ++ + D + + + Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 123 DNIGRGVSTCATCDGFFFRGKEIVVVGGGDSALEEAGFLTRFASKVTLVHRREELRASKI 182 ++ + + F G S L+ L + + + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 183 MQDRVRDNEKVAWSLNRTPLEVTSGD--RGVNGIKVLNNVTGEEEFIEASGVFVAIGHHP 240 + + +K T S G+ ++ G E + F + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 241 NTAFLGGQITTDANGYIVTNPGTSETNIPGVFACGDVQDTRYKQAITAAGSGCMAAMDAE 300 G + + + A + + Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 301 KYI 303 Sbjct: 306 LLG 308 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,441,631 Number of extensions: 951306 Number of successful extensions: 4736 Number of sequences better than 1.0e-05: 125 Number of HSP's gapped: 4632 Number of HSP's successfully gapped: 125 Length of query: 313 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 207 Effective length of database: 26,153,027 Effective search space: 5413676589 Effective search space used: 5413676589 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)