BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0167.0012.p (386 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase 318 8e-87 BS_xylR [KG] COG1940 Transcriptional regulator/sugar kinase 227 2e-59 BH0700 [KG] COG1940 Transcriptional regulator/sugar kinase 208 1e-53 BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase 204 1e-52 lin0217 [KG] COG1940 Transcriptional regulator/sugar kinase 145 9e-35 >BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 386 Score = 318 bits (815), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 159/381 (41%), Positives = 231/381 (60%), Gaps = 2/381 (0%) Query: 1 VKVTGDQALVKKINKSIILHTIRKHSPVSRARVSEMTGLNKATVSNLVSELCGQELVTEA 60 + VTGDQ +K++NK I+L TIRK +P+SRA+VS TGLNK TVS+LV+EL LV E Sbjct: 1 MSVTGDQRFIKQMNKQIVLQTIRKEAPLSRAQVSLTTGLNKGTVSSLVAELIADNLVYEV 60 Query: 61 GPGESSGGRKPLMLHFNSMAGSVIGIELQVKQLKAVLCDLGGSTLQELDCPLEVHDLPYV 120 GPGESSGGR+P+ML F + A IGI + V L VL DL G+ +++++ L V + +V Sbjct: 61 GPGESSGGRRPVMLMFQNKAAYAIGINIAVNYLSGVLTDLVGNVIEKVEWSLHVKEFDHV 120 Query: 121 LEKMKAIISELIASAPSSPYXXXXXXXXXXXXXDEHGMVLFAPNLGWEMVDLRAILEQSF 180 ++M +I +L+ +AP S Y +E G VLFAPNL W + L+ LE+ F Sbjct: 121 NQQMVQLIEQLMKAAPPSTYGIIGIGVAVPGIVNEEGDVLFAPNLNWREIPLKKQLEEHF 180 Query: 181 AVPVTIDNEANAGAQGELNFGAARDVRHLLYXXXXXXXXXXXXXXXELYKGARGYAGETG 240 +P+ ++NEANAGA GE FG ++ ++++ LY+G GY+GE G Sbjct: 181 QLPIAVENEANAGALGEKVFGKGKESENMIFFSIGIGIGSGIIFGNSLYRGKGGYSGEVG 240 Query: 241 HMTIEAEGKPCSCGSRGCWELYASERTYDNPGLALPARSTT--ELVRYAHEGQEDTLRHF 298 HM+IE G C+CG++GCWEL+ASE+ G AL T +L A +G++ + F Sbjct: 241 HMSIETNGPLCTCGNKGCWELFASEKAVLQQGEALYEEVLTLDDLFEKARKGEQTAIDLF 300 Query: 299 TTIGEYLGIGVTNLINSFNPELIVIGGALSEAEPWLGEPLRRVVAERTLPYHKQQLEITF 358 IG YLG G+ N+IN+ NPEL+V+G + + PWL L++ VA+R L +H +Q+ + F Sbjct: 301 QKIGVYLGTGIANMINALNPELVVVGNRMVKGRPWLEHSLKQTVADRALSFHAEQVLVEF 360 Query: 359 SRLGSRGTMIGAGFSAVMHFL 379 S L G +G + + FL Sbjct: 361 SDLNENGISLGVSYLLIDSFL 381 >BS_xylR [KG] COG1940 Transcriptional regulator/sugar kinase Length = 350 Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 122/345 (35%), Positives = 185/345 (53%), Gaps = 1/345 (0%) Query: 36 MTGLNKATVSNLVSELCGQELVTEAGPGESSGGRKPLMLHFNSMAGSVIGIELQVKQLKA 95 MTGLNK+TVS+ V+ L + +V E G G+SSGGR+P+ML FN AG +GI++ V + Sbjct: 1 MTGLNKSTVSSQVNTLMKESMVFEIGQGQSSGGRRPVMLVFNKKAGYSVGIDVGVDYING 60 Query: 96 VLCDLGGSTLQELDCPLEVHDLPYVLEKMKAIISELIASAPSSPYXXXXXXXXXXXXXDE 155 +L DL G+ + + LE + + + +I I P SPY D+ Sbjct: 61 ILTDLEGTIVLDQYRHLESNSPEITKDILIDMIHHFITQMPQSPYGFIGIGICVPGLIDK 120 Query: 156 HGMVLFAPNLGWEMVDLRAILEQSFAVPVTIDNEANAGAQGELNFGAARDVRHLLYXXXX 215 ++F PN W +DL++ +++ + V V I+NEANAGA GE FGAA++ +++Y Sbjct: 121 DQKIVFTPNSNWRDIDLKSSIQEKYNVSVFIENEANAGAYGEKLFGAAKNHDNIIYVSIS 180 Query: 216 XXXXXXXXXXXELYKGARGYAGETGHMTIEAEGKPCSCGSRGCWELYASERTYDNPGLAL 275 LY+G G++GE GHMTI+ G CSCG+RGCWELYASE+ Sbjct: 181 TGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLKSLQTK 240 Query: 276 PAR-STTELVRYAHEGQEDTLRHFTTIGEYLGIGVTNLINSFNPELIVIGGALSEAEPWL 334 + S +++ AH TL G YLGIG+TN++N+FNP+ +++ ++ E+ P + Sbjct: 241 EKKLSYQDIINLAHLNDIGTLNALQNFGFYLGIGLTNILNTFNPQAVILRNSIIESHPMV 300 Query: 335 GEPLRRVVAERTLPYHKQQLEITFSRLGSRGTMIGAGFSAVMHFL 379 +R V+ R E+ S LG +G + HFL Sbjct: 301 LNSMRSEVSSRVYSQLGNSYELLPSSLGQNAPALGMSSIVIDHFL 345 >BH0700 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 388 Score = 208 bits (530), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 122/374 (32%), Positives = 186/374 (49%), Gaps = 8/374 (2%) Query: 9 LVKKINKSIILHTIRKHSPVSRARVSEMTGLNKATVSNLVSELCGQELVTEAGPGESSGG 68 +KK+N+ IL I + +SR+ +S++T LNK TVS+ V+ L + L+ E ESSGG Sbjct: 8 FIKKMNRRAILEKIIEKQEISRSDLSKITNLNKVTVSSQVASLLEEHLIIEKQYAESSGG 67 Query: 69 RKPLMLHFNSMAGSVIGIELQVKQLKAVLCDLGGSTLQELDCPLEVHDLPYVLEKMKAII 128 RKP++L N A +GI+L +K L +LC+L G+ +++ L D V+ +M ++I Sbjct: 68 RKPILLTLNENAAFTLGIDLDIKTLTFMLCNLKGAVIKKEQWKLANVDFDDVVNEMISLI 127 Query: 129 SELIASAPSSPYXXXXXXXXXXXXXDEHGMVLFAPNLGWEMVDLRAILEQSFAVPVTIDN 188 + P+S Y D + F P W V+++ +EQ+ +PV ++N Sbjct: 128 QSFLKDIPASKYGVVGIGIGIHGIVDNDETIAFTPGHHWSNVNVKQPIEQAVNIPVVVEN 187 Query: 189 EANAGAQGELNFGAARDVRHLLYXXXXXXXXXXXXXXXELYKGARGYAGETGHMTIEAEG 248 N A E N + + +LL +YKG GYAGE GHM I EG Sbjct: 188 STNLCALAE-NVVSDHEADNLLCISSFSGVGMGLIVHNAIYKGFHGYAGEVGHMIIVPEG 246 Query: 249 KPCSCGSRGCWELYASERTYDNPGLALPARSTTE---LVRYAHEGQEDTLRHFTTIGEYL 305 KPC CG++GCWELYASE+ R + ++ G E+TL+ Y Sbjct: 247 KPCRCGNKGCWELYASEKALYQEISESTKREHVDRDVVLALIEAGDEETLKKLDQFIYYF 306 Query: 306 GIGVTNLINSFNPELIVIGGALSEAEPWLGEPLRRVVAERTLPYHKQQLEITFSRLGSRG 365 +G+ N+INS+NPE IVI L P + ++ + + H + L+I S LG Sbjct: 307 SLGLNNIINSYNPETIVISSGLMPHLPNIEHKIQSHLHSKI--SHYKTLKI--SNLGKEA 362 Query: 366 TMIGAGFSAVMHFL 379 +GA F + FL Sbjct: 363 CALGACFIGIKQFL 376 >BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 407 Score = 204 bits (520), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 128/390 (32%), Positives = 196/390 (50%), Gaps = 25/390 (6%) Query: 3 VTGDQALVKKINKSIILHTIRKHSPVSRARVSEMTGLNKATVSNLVSELCGQELVTEAGP 62 V G L+K +N+S+IL+TIR P+SRA +++ T L TV+N+V+EL ++LV E+ Sbjct: 3 VAGSFQLMKSMNRSLILNTIRLDGPISRADIAKKTKLTPPTVTNIVAELIEEKLVRESKM 62 Query: 63 GESSGGRKPLMLHFNSMAGSVIGIELQVKQLKAVLCDLG----GSTLQELDCPLEVHDLP 118 GES GGRKP++L ++ VIG+++ +++AVL DL G T EL L L Sbjct: 63 GESRGGRKPILLTIDAKNSFVIGLDVGGYRVRAVLTDLNGEVEGQTTVELSSDLTEETL- 121 Query: 119 YVLEKMKAIISELIASAPSSPYXXXXXXXXXXXXXD-EHGMVLFAPNLGWEMVDLRAILE 177 ++++ I L D + GM +FAPN +DL+ E Sbjct: 122 --IDQLIKITVTLFEQCKMQKDKVIGIGIGMHGIVDHQSGMAVFAPNFQLTNIDLKTPFE 179 Query: 178 QSFAVPVTIDNEANAGAQGELNFGAARDVRHLLYXXXXXXXXXXXXXXXELYKGARGYAG 237 +F VPV ++N+A A A GE FG + + H++ L+ G G AG Sbjct: 180 STFDVPVFVENDARALALGETWFGNGQGIDHVICVNVGVGIGAGIVLNKRLFHGVHGIAG 239 Query: 238 ETGHMTIEAEGKPCSCGSRGCWELYASERTYDNPGLALPARSTTELVRYAHEGQED---- 293 E GH T++ GK C+CG+ GC + A + A ++ EG D Sbjct: 240 EIGHTTVDIHGKKCTCGNYGCLQTVAGGNMLKERAIHDIASGRKTMLLEKVEGDMDRISG 299 Query: 294 -------------TLRHFTTIGEYLGIGVTNLINSFNPELIVIGGALSEAEPWLGEPLRR 340 ++ G+YLGIG+TN+IN NP+ I++GG +S+A ++ EPLR+ Sbjct: 300 EIIHQCALAGDPLSIEILEEAGKYLGIGITNIINFINPDRIIVGGGVSKAGDFVLEPLRK 359 Query: 341 VVAERTLPYHKQQLEITFSRLGSRGTMIGA 370 +V ER L ++ E+ + LG GT IGA Sbjct: 360 MVKERALTAKARETEVVTAELGEWGTAIGA 389 >lin0217 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 404 Score = 145 bits (367), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 94/334 (28%), Positives = 156/334 (46%), Gaps = 16/334 (4%) Query: 5 GDQALVKKINKSIILHTIRKHSPVSRARVSEMTGLNKATVSNLVSELCGQELVTEAGPGE 64 G++ L+K IN+ +L+ IR+ ++R +++ +T++ ++ +L + ++ E Sbjct: 7 GNKDLIKDINRYTVLNLIREKGEITRTEIAKKCDFGMSTLTYILDDLQQEGIILEGAETS 66 Query: 65 SSGGRKPLMLHFNSMAGSVIGIELQVKQLKAVLCDLGGSTLQELDCPLEVHDLPYVLEKM 124 S+GGR+ ++ FN G V+ ++++ +QL L DL ++ P P E Sbjct: 67 STGGRRAKLVRFNKDYGFVVSVKVEEEQLLFALTDLNAEIIKNTSIPFSSEKKP---EDA 123 Query: 125 KAIISELIASAPSSPYXXXXX---XXXXXXXXDEHGMVLFAPNLGWEMVDLRAILEQSF- 180 +I+E + + + G V+ + LGWE V L A+L F Sbjct: 124 IDLIAENVKKMCKNRDMKNLLGVGIAISGLVNRKKGTVIRSTMLGWENVALEAMLHVHFP 183 Query: 181 AVPVTIDNEANAGAQGELNFGAARDVRHLLYXXXXXXXXXXXXXXXELYKGARGYAGETG 240 +PV +D N EL G + + ++Y GA+G AGE G Sbjct: 184 DIPVYVDKNINCYTLAELWLGEGKQSNNFATVSVGAGLGLSVVINRQIYYGAQGGAGEFG 243 Query: 241 HMTIEAEGKPCSCGSRGCWELYASERTYDNPGLALP-ARSTTEL--------VRYAHEGQ 291 H TI+ G C CG +GC E+YASE + N G + A T+EL + A G Sbjct: 244 HTTIQPGGYKCHCGQKGCLEMYASEFYFRNRGEEIKEAFPTSELNDFHFDNVAKSARAGD 303 Query: 292 EDTLRHFTTIGEYLGIGVTNLINSFNPELIVIGG 325 E + +GEYLG G+ N+IN+FNPE ++I G Sbjct: 304 EMAIELMGKMGEYLGYGIRNIINTFNPEKVIIVG 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,053,722 Number of extensions: 976566 Number of successful extensions: 2691 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 2582 Number of HSP's successfully gapped: 86 Length of query: 386 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 278 Effective length of database: 25,922,359 Effective search space: 7206415802 Effective search space used: 7206415802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)