BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0008.p (326 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ycxC [GER] COG0697 Permeases of the drug/metabolite transport... 223 3e-58 CAC1538 [GER] COG0697 Permeases of the drug/metabolite transport... 204 2e-52 TM1017 [GER] COG0697 Permeases of the drug/metabolite transporte... 129 6e-30 AF0266 [GER] COG0697 Permeases of the drug/metabolite transporte... 66 6e-11 BS_ybfH [GER] COG0697 Permeases of the drug/metabolite transport... 54 3e-07 >BS_ycxC [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 312 Score = 223 bits (568), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 134/300 (44%), Positives = 195/300 (65%), Gaps = 1/300 (0%) Query: 18 AYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKP 77 AYI A+L + IIG SFLF K+AL A P D L +RF +F +VP+ FGWVK+S K Sbjct: 9 AYIAAILYSFIIGLSFLFVKIALQTAEPFDILAHRFTIAFAAATVPILFGWVKLSILVKD 68 Query: 78 IGRALLLAAMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKL 137 + L LA +YP FF+ Q FGL +++S+E GI+ + P+ TM+ A+ LKE T QK Sbjct: 69 VIDILPLALLYPALFFSFQAFGLVYSSSSEAGIIQAAIPIFTMVFAAYVLKERPTWTQKG 128 Query: 138 CIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYL 197 LSV GV+FIFVMKG ++ +++ G +L+ L+ L+ A Y+ AR +++ F EL+Y+ Sbjct: 129 FTVLSVAGVMFIFVMKGVDVESASLKGSLLILLSALSSAMYNTAARKMTQRFKLTELTYI 188 Query: 198 LMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSK 257 + IGF F ++L H A+GT+G + P + F+L+ VYLGV++SLVT+ S Y LS+ Sbjct: 189 MSAIGFVVFNAIALVRHGAAGTVGTYFLPFREPGFVLAIVYLGVLSSLVTSFLSNYTLSR 248 Query: 258 IEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNRLGRKKTEARPG 317 IEA MS F+++STIV++ AG + L E + YH G+ I+ GV+G+N KKT+ RPG Sbjct: 249 IEAFKMSAFNHVSTIVTMIAGFVILNESLAWYHLAGAVCIMIGVVGSNINLEKKTK-RPG 307 >CAC1538 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 299 Score = 204 bits (519), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 121/297 (40%), Positives = 180/297 (60%) Query: 10 MQQQGLKLAYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWV 69 M + L YI A +++ I G SFLF+K+AL AGPL + RF +F++M++ V F + Sbjct: 1 MNGKQKILPYIAATVSSTIFGLSFLFSKMALKVAGPLSLVALRFLLAFIIMTILVLFRVI 60 Query: 70 KVSYRGKPIGRALLLAAMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKE 129 KV YR KPIG+ +LL P+ +F + +G+++ +S+ G++ + P+V I+ S FLKE Sbjct: 61 KVDYRNKPIGQLVLLGLAEPVIYFIFETYGVKNISSSMAGLMLALIPIVVTIMGSYFLKE 120 Query: 130 TTTLLQKLCIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHF 189 +L + I LSV GV I VM S ++ GI+ L YS+++R S HF Sbjct: 121 VPSLKRTSFIVLSVSGVALIGVMGSSGGSGNSPIGILFLLGAVTCAGAYSIISRKTSVHF 180 Query: 190 SPAELSYLLMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTL 249 P E++Y +M F I++ A G + + SPLKS TF++S +YLG+++S+V Sbjct: 181 KPVEITYFMMFFAAFCFNIMAAIQLLAEGKINEYFSPLKSSTFVISILYLGILSSIVAYF 240 Query: 250 TSTYILSKIEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVIGTNR 306 + + LSKI+AS SVFSNLSTIVSI AG + L E +YH +GS LI+ GV GTN+ Sbjct: 241 LTNFTLSKIQASRSSVFSNLSTIVSIIAGVIILKESFHLYHLVGSVLILTGVWGTNK 297 >TM1017 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 293 Score = 129 bits (324), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 90/277 (32%), Positives = 145/277 (52%), Gaps = 13/277 (4%) Query: 26 AVIIGFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKPIGRALLLA 85 + I G SFLFTK AL + PL L++RF +F+ + + G VK+ KP + L Sbjct: 13 STIFGLSFLFTKNALDYVTPLTFLSFRFIVAFLSYLLLLITGAVKLG--KKPYWKLWKLV 70 Query: 86 AMYPLGFFTLQVFGLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKLCIFLSVFG 145 P+ +F + +GLQ S+E G++ + P+V ++A LKE LL L + + G Sbjct: 71 LFQPVLYFLFETYGLQRVNSSEAGMIIALIPIVVNLLAPFILKEKGDLLHYLLVGMGFLG 130 Query: 146 VVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYLLMGIGFAT 205 V I G +I N+ G V + L L+ A YSV +R S+ F+P E+++ +M G Sbjct: 131 VSLIV---GFNITPGNIVGKVFMLLAVLSGAMYSVFSRKFSKEFTPTEITFFMMMTGAVF 187 Query: 206 FLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSKIEASLMSV 265 F +LSL+ G P+ + ++ A+YLGV++S V Y + K+ ++ Sbjct: 188 FTLLSLST--------GDFRPVFNVDVVIGALYLGVLSSTVAFFLLNYAIRKLSPIFTTL 239 Query: 266 FSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGVI 302 FSN +T+VS+ AG +F E + + G LII+ +I Sbjct: 240 FSNFTTVVSVIAGVVFRNETVGIQQIAGMGLIISSLI 276 >AF0266 [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 276 Score = 66.2 bits (160), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 75/281 (26%), Positives = 134/281 (47%), Gaps = 35/281 (12%) Query: 30 GFSFLFTKVALGHAGPLDTLTYRFAASFVVMSVPVAFGWVKVSYRGKPIGRALLLAAMYP 89 G +F K ++ P+ + RFA + + +V + G ++ ++ P ++ Sbjct: 15 GLAFTAIKYSVRFLSPIAIASLRFAIANTLFAVIIILG-KRIKWKDLP--------KVFA 65 Query: 90 LGFFTLQVF------GLQHATSAEGGILYSFTPVVTMIIASVFLKETTTLLQKLCIFLSV 143 LG F + V+ G + +S ++ S P+ +I++++FL+E T + + I ++ Sbjct: 66 LGIFGVSVYHVFLNLGEVYISSGVASVVISLAPIFVLILSAIFLRERITYSKVVGIIIAF 125 Query: 144 FGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARSLSRHFSPAELSYLLMGIG- 202 GVV I S +N+ GI L+ ++ +A A Y+ +SL ++P L+ M +G Sbjct: 126 LGVVVI-----SEPSYANIYGIALVMVSTVAAAIYTTFGKSLLSKYNPITLTSNAMVLGS 180 Query: 203 --FATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGVIASLVTTLTSTYILSKIEA 260 FL S+ +LGG L+ I S V+LG+ ++ L Y L K EA Sbjct: 181 IPLYPFLPDSIR------SLGGDLN------LIGSIVFLGIFSTFFGYLGWYYFLEKEEA 228 Query: 261 SLMSVFSNLSTIVSIAAGALFLGEEITVYHWIGSFLIIAGV 301 S SVF +VS+ AG + L E +T+ GS L++ G+ Sbjct: 229 SRASVFLLAIPVVSLLAGNILLAEPLTLRTVAGSGLVLLGI 269 >BS_ybfH [GER] COG0697 Permeases of the drug/metabolite transporter (DMT) superfamily Length = 105 Score = 54.3 bits (129), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 0/291 (0%), Positives = 0/291 (0%), Gaps = 25/291 (8%) Query: 10 MQQQGLKLAYIFAVLNAVIIGFSFLFTKVALGHAGPLDTLTYRFAASFV--VMSVPVAFG 67 M + + I F + VA + + + Sbjct: 1 MDKVIFYSNIRNLIFKKCSIQFLIINDTVAHKEQVAMREMNQLVNTEDSAWPIIQNW--- 57 Query: 68 WVKVSYRGKPIGRALLLAAMYPLG---FFTLQVFGLQHATSAEGGILYSFTPVVTMIIAS 124 L + Sbjct: 58 --LKDATNHT--ELLPVNKDLAETALY 113 Query: 125 VFLKETTTLLQKLCIFLSVFGVVFIFVMKGSSIQLSNMTGIVLLFLTCLAFAGYSVLARS 184 Sbjct: 114 173 Query: 185 LSRH-FSPAELSY--LLMGIGFATFLILSLTGHAASGTLGGFLSPLKSGTFILSAVYLGV 241 Sbjct: 174 ------- 226 Query: 242 IASLVTTLTSTYILSKIEASLMSVFSNLSTIVSIAAGALFLGEEITVYHWI 292 Sbjct: 227 --- 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.141 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,155,448 Number of extensions: 819261 Number of successful extensions: 3273 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 3276 Number of HSP's successfully gapped: 42 Length of query: 326 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 219 Effective length of database: 26,037,693 Effective search space: 5702254767 Effective search space used: 5702254767 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)