BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0005.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ywcG [C] COG0778 Nitroreductase 196 4e-50 BH1048 [C] COG0778 Nitroreductase 181 8e-46 lin0935 [C] COG0778 Nitroreductase 171 9e-43 SA0367 [C] COG0778 Nitroreductase 166 3e-41 BS_ycnD [C] COG0778 Nitroreductase 159 3e-39 >BS_ywcG [C] COG0778 Nitroreductase Length = 346 Score = 196 bits (497), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 13/245 (5%), Positives = 30/245 (12%), Gaps = 2/245 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 + EQ + Q+ + +A P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 + + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 L V + + G + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 185 LHHNGY--QADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFL 242 A + + Y + G L Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 243 LQKGF 247 K Sbjct: 242 TGKYL 246 >BH1048 [C] COG0778 Nitroreductase Length = 346 Score = 181 bits (459), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 12/243 (4%), Positives = 37/243 (15%), Gaps = 4/243 (1%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 + + S ++ A A + + + V + + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 L+ + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRN--NDKGIRPDQALDRKNIREALHDSLKR 120 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 + + N +L + + R + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 185 LHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQ 244 G +D ++ + + + L L Sbjct: 181 ETAFGVMRYLH--LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 245 KGF 247 Sbjct: 239 GTL 241 >lin0935 [C] COG0778 Nitroreductase Length = 346 Score = 171 bits (433), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 8/247 (3%), Positives = 29/247 (11%), Gaps = 3/247 (1%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 + EQ + Q+ + +A P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSR-NNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYP-AGVNGIRPRLPLQA 183 + + N +L + G + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 184 VLHHNGYQADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLL 243 + + + + ++ Sbjct: 181 ETAFGVMR-YLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 244 QKGFMQK 250 Sbjct: 240 TLTGKYL 246 >SA0367 [C] COG0778 Nitroreductase Length = 346 Score = 166 bits (420), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 12/245 (4%), Positives = 32/245 (13%), Gaps = 5/245 (2%) Query: 10 LLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLSKLSGN 69 + S ++ A A + + + V + + + L+ Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE- 66 Query: 70 QAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXXXXXXE 129 + + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 130 SLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPA-GVNG-IRPRLPLQAVLHH 187 L V + + + + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 188 NGYQADASVE--QVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLLQK 245 Y A V + + LA Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 246 GFMQK 250 + Sbjct: 247 NGAKP 251 >BS_ycnD [C] COG0778 Nitroreductase Length = 346 Score = 159 bits (402), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 9/244 (3%), Positives = 32/244 (13%), Gaps = 1/244 (0%) Query: 5 NETLELLNRHTSVRQFQDKPVSDEQLAAIIGAGQMASTSSNVQAYTVIAVTEPDLKAQLS 64 + EQ + Q+ + +A P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 65 KLSGNQAYIEQCPVFLVWCADLYRLREISGPHLQGAASYVGSTENLIVXXXXXXXXXXXX 124 + + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 125 XXXXESLGLGIVYIGGVRNNIAELSELLGLPELVYPVFGMCLGYPAGVNGIRPRLPLQAV 184 L V + + G + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 185 LHHNGY-QADASVEQVKVYDEVTRDYMRERTGGQKDTPWSEMMAGRLAQPSRLQMKEFLL 243 + + ++ + + + + L Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 244 QKGF 247 + Sbjct: 242 TGKY 245 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,239,664 Number of extensions: 681827 Number of successful extensions: 1208 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 1154 Number of HSP's successfully gapped: 34 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)