BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0002.p (383 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvfT [T] COG4585 Signal transduction histidine kinase 299 6e-81 BS_yocF [T] COG4585 Signal transduction histidine kinase 202 7e-52 BH0582 [T] COG4585 Signal transduction histidine kinase 182 6e-46 DRA0009 [T] COG4585 Signal transduction histidine kinase 125 1e-28 SA1158 [T] COG4585 Signal transduction histidine kinase 125 2e-28 >BS_yvfT [T] COG4585 Signal transduction histidine kinase Length = 328 Score = 299 bits (765), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 159/337 (47%), Positives = 225/337 (66%), Gaps = 17/337 (5%) Query: 45 MLALFAVTYRQLYWATGRA-FTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGE 103 ML LF YRQL+ + G+A FT WL VQM +I++ S Y +++GFF ANF+G+Y + Sbjct: 1 MLLLFVAAYRQLFCSVGKASFTYWLIVQMAVILMYSVFYNITYIYLGFFPANFVGYYKEK 60 Query: 104 KHYKMAYAVFAAMVLVLLITATW-----GMALEDLLFLYPFMIIMLVSPFGIRSLHRKRM 158 ++ A F A++ +LL + ++L +L + PF++IML+SPFGIRS+ R+ Sbjct: 61 TNFNRA---FCALIFILLFPCLYQFIANSVSLRELFSVLPFLVIMLISPFGIRSMFRRIE 117 Query: 159 LEKELDQANEVIKELVKREERMRIARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAE 218 LE +L QANE IKEL KREER+RIARDLHDTLGHTLSL+TLKSQL+++LAA D RT+ E Sbjct: 118 LEAKLAQANEQIKELSKREERVRIARDLHDTLGHTLSLLTLKSQLIQRLAASDPERTKLE 177 Query: 219 AREIQRISRAALRQVRELVSEMRAVSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLT 278 A+E++ SR+AL+QVRELVS+MR V++ EEL + +LR +I + EG +S +T Sbjct: 178 AKEMETSSRSALKQVRELVSDMRTVTITEELVNIQHILRAGNITFQYEGADDFSVISPVT 237 Query: 279 QNILSLCIKEAVTNIVKHSGADRCRIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGN 338 QNI+S+C++EAVTNI+KHS A C I ++ +++I I D+G G P+E + GN Sbjct: 238 QNIISMCMREAVTNIIKHSKATHCAITISQFADKMRIVIRDDGKG-APKE-----KMFGN 291 Query: 339 GMKGMAERLSLIDXXXXXXXXXXXXTVLAVAIPRVVK 375 G+ GM ERL LI+ TV+A+ IP + K Sbjct: 292 GLWGMEERLMLIE--GGLTVSDHNGTVVALTIPLIKK 326 >BS_yocF [T] COG4585 Signal transduction histidine kinase Length = 370 Score = 202 bits (514), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 132/355 (37%), Positives = 197/355 (55%), Gaps = 19/355 (5%) Query: 1 MARRQFRLFPQRFGFFPYMWLIYLLFPVINL-QEEHGIKLVFGAAMLALFAVTYRQLYWA 59 M + F F + G PY+W I+ + P + + ++ G + LF YR + + Sbjct: 1 MIKNHFT-FQKLNGITPYIWTIFFILPFYFIWKSSSTFVIIVGIILTLLFFSVYRFAFVS 59 Query: 60 TGRAFTLWLAVQMLIIVILSAAYTPYD-LFMGFFTANFIGWYTGEKHYKMAYAVFAAMVL 118 G LW L+I I +A+ T + ++ FF A FIG + + Y V ++ Sbjct: 60 KGWTIYLW---GFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYV---HLI 113 Query: 119 VLLITATWGMALEDLLFL--YPFMIIMLVS----PFGIRSLHRKRMLEKELDQANEVIKE 172 + A + + L+ FL PF++I L+S PF I+S + LE++L+ ANE I E Sbjct: 114 SAAVAANFSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAE 173 Query: 173 LVKREERMRIARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQ 232 LVK EER RIARDLHDTLG LSLI LKS L KL KD + E + +Q+ +R +L + Sbjct: 174 LVKLEERQRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNE 233 Query: 233 VRELVSEMRAVSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTN 292 VR++VS M+ + + +EL + ++L ADI E + E +S L +NILS+C+KEAVTN Sbjct: 234 VRKIVSSMKGIRLKDELINIKQILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTN 293 Query: 293 IVKHSGADRCRIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERL 347 +VKHS A CR+ + EV I++ D+G + E + SKG+G+ GM ERL Sbjct: 294 VVKHSQAKTCRVDIQQLWKEVVITVSDDGTF----KGEENSFSKGHGLLGMRERL 344 >BH0582 [T] COG4585 Signal transduction histidine kinase Length = 374 Score = 182 bits (463), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 106/311 (34%), Positives = 168/311 (54%), Gaps = 14/311 (4%) Query: 44 AMLALFAVTYRQLYWATGRAFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGE 103 ++LA F TY + W I + + Y P+ L GF ++F+G Sbjct: 43 SLLAAFLFTYYMMLWYKDIRLLFAALTACFIFALFAIFYKPWLLLYGFIVSDFLG----R 98 Query: 104 KHYKMAYAVFAAMVLVLLITATWGMALEDLLFL----YPFMIIMLVSPFGIRSLHRKRML 159 H K + + + I+ + + F P +I+ LV PF IR + ++L Sbjct: 99 AHAKRLMWIGVVGLFAMHISTAYALYGHWFTFYSPVQLPILILQLVLPFMIRIREKSKLL 158 Query: 160 EKELDQANEVIKELVKREERMRIARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEA 219 ++ELD AN+ ++ ++ EER RIARDLHDTLG +L++IT+KSQL +L KDA + + E Sbjct: 159 KEELDTANQKLERYIQEEERHRIARDLHDTLGQSLTMITMKSQLATRLVEKDADQAKKEM 218 Query: 220 REIQRISRAALRQVRELVSEMRAVSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQ 279 E+ SR L+QVRELV+ M+ VS+ EE++ E+L +I +EV + A + + + Sbjct: 219 TEVMNTSRRVLKQVRELVTTMKFVSLEEEVSHAKEILSLQNITLEVIQEGATPQLLKVAE 278 Query: 280 NILSLCIKEAVTNIVKHSGADRCRIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNG 339 ++L+L ++EAVTNI+KHS A C I E ++ + DNG+G Q Q GNG Sbjct: 279 SMLALSLREAVTNIIKHSRATHCTIVKEYANNEYKLQVIDNGIGKIDQMQ------TGNG 332 Query: 340 MKGMAERLSLI 350 + + ER+ L+ Sbjct: 333 ITSIRERIHLL 343 >DRA0009 [T] COG4585 Signal transduction histidine kinase Length = 336 Score = 125 bits (314), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 97/285 (34%), Positives = 142/285 (49%), Gaps = 16/285 (5%) Query: 63 AFTLWLAVQMLIIVILSAAYTPYDLFMGFFTANFIGWYTGEKHYKMAYAVFAAMVLVLLI 122 A+ WL+ +L+ + Y P L + + IGW + A+ A + LL+ Sbjct: 28 AYVGWLSA-LLMWALGDWLYGPSALSFLVYGGSLIGW-----QRRQWVALGGAAINALLM 81 Query: 123 TATWGMALEDLLFLYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRI 182 E + F++ +L S + + +R + L Q ++L ER RI Sbjct: 82 VGRLLTLPERSQGDFIFLLFILASAYANHATYRGLRARRRLAQVQLEKEKLAADAERERI 141 Query: 183 ARDLHDTLGHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRA 242 ARDLHD LGHTLS+I LKS+L KLA K R AE RE++RISR AL +VR V R Sbjct: 142 ARDLHDLLGHTLSVIVLKSELAGKLAEKHPQRAAAEIREVERISREALSEVRAAVQGYRG 201 Query: 243 VSVAEELAEVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRC 302 +A ELA L A I + + D E +DL ++ L++ ++EAVTN+V+HSGA Sbjct: 202 SGLAAELARAKVALDAAGIRLVIT-DTLPELPADL-ESTLAMVLREAVTNVVRHSGASEL 259 Query: 303 RIALAVTEGEVQISIEDNGVGLHPQEQEGHGRSKGNGMKGMAERL 347 R+ L Q++++D+G G E G G+ M ERL Sbjct: 260 RLTLRGQANGFQLTLQDDGRGGDAPE--------GTGLNSMRERL 296 >SA1158 [T] COG4585 Signal transduction histidine kinase Length = 363 Score = 125 bits (313), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 89/315 (28%), Positives = 166/315 (52%), Gaps = 17/315 (5%) Query: 21 LIYLLFPV--INLQEEHGIKLVFGAAMLALFAVTYRQLYWATGR----AFTLWLAVQMLI 74 ++YL+FP+ I E +G K ++ +++ +F+++Y L R F + L + I Sbjct: 15 ILYLIFPIAGIFFNEVYGPKWLYIISVI-VFSLSYLILVIVNNRLNTLMFYILLIIHYFI 73 Query: 75 IVILSAAYTPYDLFMGFFTANFIGWYTGEKHYK-MAYAVFAAMVLVLLITATWGMALEDL 133 I + P L + FF + F +T + + K A +F +L ++I L + Sbjct: 74 ICYFVFSVHPM-LSLFFFYSAFAVPFTFKNNVKKTATNLF---ILTMIICTIITYLLYNN 129 Query: 134 LF---LYPFMIIMLVSPFGIRSLHRKRMLEKELDQANEVIKELVKREERMRIARDLHDTL 190 F + +++I L+ + + + R +KE+ + N I L+ +ER RI +DLHDTL Sbjct: 130 YFVAMMVYYVVISLIMLDNFKKM-KNREYQKEIAEKNRHINTLIAEQERHRIGQDLHDTL 188 Query: 191 GHTLSLITLKSQLVEKLAAKDAGRTQAEAREIQRISRAALRQVRELVSEMRAVSVAEELA 250 GH + ++LKS+L KL D + +AE I ++SR +L +VRE++ +++ S EE+ Sbjct: 189 GHVFASLSLKSELAYKLIDTDVEKVKAELLAINKLSRESLNKVREIIDDVKLPSFIEEID 248 Query: 251 EVGEMLRTADIAMEVEGDAALEGVSDLTQNILSLCIKEAVTNIVKHSGADRCRIAL-AVT 309 + ++L+ ADI E + +S Q++L + +EA+ N++KH+ A + L V Sbjct: 249 SIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKVHGKLKTVN 308 Query: 310 EGEVQISIEDNGVGL 324 ++ + IED+G G+ Sbjct: 309 NHKLLLMIEDDGKGI 323 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,411,962 Number of extensions: 906604 Number of successful extensions: 5259 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 5207 Number of HSP's successfully gapped: 56 Length of query: 383 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 275 Effective length of database: 25,922,359 Effective search space: 7128648725 Effective search space used: 7128648725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)